MyleA.00141.a

MALONYL CoA-ACYL CARRIER PROTEIN TRANSACYLASE FABD (Malonyl CoA:AcpM acyltransferase) (MCT)

CENTER ID: MyleA.00141.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00141.a.A1.GE34171 full length 1 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3131
RefSeq: YP_002503764.1
UniProt: A0A0H3MR64

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIALLAPGQG SQTEGMLSPW LGLPGAADQI ATWSKASGLD LVRLGTTAST EEITDTAVAQ PLIVATTLLA HQELAKHALF ENALDNLIVA GHSVGEIAAY SIAGVIAADD AVALAATRGA EMAKACAIEP TGMSAVLGGD EADVLNRLEE LDLVPANRNA AGQIVAAGRL TALEKLAEDP PARARVRALD VAGAFHTEFM TAALDGFAEA AASITIAEPI AMLLSNRDGK PVASAATGME TLISQLTHPA RWDLCNVTLR EHNVTAIVEL PPAGALTGIA KRELREVTTC AVKSPTDLDK LANL
NT Sequence
gtgattgcgt tacttgcacc cggacagggc tcgcagaccg agggaatgct atcgccgtgg ctgggactcc ccggtgccgc agaccagatc gcgacctggt cgaaggcaag tggcttagat ctggtacgtc taggcaccac cgcctcgacc gaggagataa ccgataccgc tgtcgctcag ccgttgattg ttgcgaccac gctgctagct catcaggagc tggccaagca tgccttgttt gagaatgccc tcgacaacct aatcgtggcc ggacactccg tcggcgagat cgcggcgtat tcgatcgccg gtgtgatcgc cgccgatgat gcagtggcgc tggccgcaac ccgcggcgcc gagatggcca aggcatgcgc catcgagccg accggcatgt ccgctgtgct cggcggtgac gaagccgacg tgctgaatcg cctcgaggaa cttgacctgg ttcctgcgaa ccgcaacgcc gccggtcaga ttgtcgccgc cggtcgactg accgcgctag aaaaactcgc cgaagacccg ccagccaggg cccgggtgcg agcactggac gtggccggag cgttccacac cgagttcatg accgcagctc ttgatggctt cgcagaggcg gcggccagca tcacgattgc cgaacccatc gcgatgctgc tctccaaccg tgacggaaaa ccggtcgcgt cggcagcgac cggtatggaa accctgattt ctcagctgac tcacccggcg cgctgggatc tgtgcaacgt aacgctgcgt gagcacaacg tcacggcgat cgtggaactc ccgcccgcag gcgctctcac cggaatcgcc aaacgcgagc ttcgggaagt aacaacttgc gccgtcaagt cgcccacaga cctggacaag ttggcgaacc tctaa
Details for MyleA.00141.a.A1.GE34171
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/13/2011
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVIALLAPG QGSQTEGMLS PWLGLPGAAD QIATWSKASG LDLVRLGTTA STEEITDTAV AQPLIVATTL LAHQELAKHA LFENALDNLI VAGHSVGEIA AYSIAGVIAA DDAVALAATR GAEMAKACAI EPTGMSAVLG GDEADVLNRL EELDLVPANR NAAGQIVAAG RLTALEKLAE DPPARARVRA LDVAGAFHTE FMTAALDGFA EAAASITIAE PIAMLLSNRD GKPVASAATG METLISQLTH PARWDLCNVT LREHNVTAIV ELPPAGALTG IAKRELREVT TCAVKSPTDL DXLANL
Validated NT Sequence
ttgttnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgattg cgttacttgc acccggacag ggctcgcaga ccgagggaat gctatcgccg tggctgggac tccccggtgc cgcagaccag atcgcgacct ggtcgaaggc aagtggctta gatctggtac gtctaggcac caccgcctcg accgaggaga taaccgatac cgctgtcgct cagccgttga ttgttgcgac cacgctgcta gctcatcagg agctggccaa gcatgccttg tttgagaatg ccctcgacaa cctaatcgtg gccggacact ccgtcggcga gatcgcggcg tattcgatcg ccggtgtgat cgccgccgat gatgcagtgg cgctggccgc aacccgcggc gccgagatgg ccaaggcatg cgccatcgag ccgaccggca tgtccgctgt gctcggcggt gacgaagccg acgtgctgaa tcgcctcgag gaacttgacc tggttcctgc gaaccgcaac gccgccggtc agattgtcgc cgccggtcga ctgaccgcgc tagaaaaact cgccgaagac ccgccagcca gggcccgggt gcgagcactg gacgtggccg gagcgttcca caccgagttc atgaccgcag ctcttgatgg cttcgcagag gcggcggcca gcatcacgat tgccgaaccc atcgcgatgc tgctctccaa ccgtgacgga aaaccggtcg cgtcggcagc gaccggtatg gaaaccctga tttctcagct gactcacccg gcgcgctggg atctgtgcaa cgtaacgctg cgtgagcaca acgtcacggc gatcgtggaa ctcccgcccg caggcgctct caccggaatc gccaaacgcg agcttcggga agtaacaact tgcgccgtca agtcgcccac agacctggac nagttggcga acctctaata aacagcacga annntnctgc anccnagctt cnnnnnngnn ccngctncta ncnaanccgn anggnagctg nnnngctnct nnnnnnnann nnnnnnnnnn nnnnntnnnn nnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIALLAPG QGSQTEGMLS PWLGLPGAAD QIATWSKASG LDLVRLGTTA STEEITDTAV AQPLIVATTL LAHQELAKHA LFENALDNLI VAGHSVGEIA AYSIAGVIAA DDAVALAATR GAEMAKACAI EPTGMSAVLG GDEADVLNRL EELDLVPANR NAAGQIVAAG RLTALEKLAE DPPARARVRA LDVAGAFHTE FMTAALDGFA EAAASITIAE PIAMLLSNRD GKPVASAATG METLISQLTH PARWDLCNVT LREHNVTAIV ELPPAGALTG IAKRELREVT TCAVKSPTDL DKLANL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgattgcg ttacttgcac ccggacaggg ctcgcagacc gagggaatgc tatcgccgtg gctgggactc cccggtgccg cagaccagat cgcgacctgg tcgaaggcaa gtggcttaga tctggtacgt ctaggcacca ccgcctcgac cgaggagata accgataccg ctgtcgctca gccgttgatt gttgcgacca cgctgctagc tcatcaggag ctggccaagc atgccttgtt tgagaatgcc ctcgacaacc taatcgtggc cggacactcc gtcggcgaga tcgcggcgta ttcgatcgcc ggtgtgatcg ccgccgatga tgcagtggcg ctggccgcaa cccgcggcgc cgagatggcc aaggcatgcg ccatcgagcc gaccggcatg tccgctgtgc tcggcggtga cgaagccgac gtgctgaatc gcctcgagga acttgacctg gttcctgcga accgcaacgc cgccggtcag attgtcgccg ccggtcgact gaccgcgcta gaaaaactcg ccgaagaccc gccagccagg gcccgggtgc gagcactgga cgtggccgga gcgttccaca ccgagttcat gaccgcagct cttgatggct tcgcagaggc ggcggccagc atcacgattg ccgaacccat cgcgatgctg ctctccaacc gtgacggaaa accggtcgcg tcggcagcga ccggtatgga aaccctgatt tctcagctga ctcacccggc gcgctgggat ctgtgcaacg taacgctgcg tgagcacaac gtcacggcga tcgtggaact cccgcccgca ggcgctctca ccggaatcgc caaacgcgag cttcgggaag taacaacttg cgccgtcaag tcgcccacag acctggacaa gttggcgaac ctctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa