SSGCID: Target Page

MyleA.00118.a

ALPHA-D-GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE RMLA (DTDP-GLUCOSE SYNTHASE) (DTDP-GLUCOSE PYROPHOSPHORYLASE)

CENTER ID:	MyleA.00118.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00118.a.A1.GE34154	full length		1	288

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.4828
RefSeq:	YP_002504248.1
UniProt:	A0A0H3N128

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRGIILAGGS GTRLYPITLG ISKQLLPVYD KPMIYYPLST LMMAGIRDIL VITTAHDAPG FKRLLGDGTQ FGVNISYATQ DHPDGLAQAF VIGANHIGAD TVALVLGDNI FYGPGLGTSL RRFQYVSGGA IFAYCVANPS SYGIVELGID GIALSLEEKP ATPKSQYAVP GLYFYDNDVV EIARGLTKSA RGEYEITEVN QIYLNQGRLT VEVLARGTAW LDTGTFDSLL DASDFVRTLE RRQGLKVSVP EEVSWRMGWI DDEQLALRAH SLAKSGYGCY LSELLERG

NT Sequence

atgcggggga ttatcttggc tggcggctct ggtacacggc tgtacccaat cacgctgggc attagcaagc agttattgcc ggtatacgac aaacctatga tttattatcc gctgtccacg ctgatgatgg ccggcatccg cgacatcttg gtgattacca ccgctcacga cgcacccggg tttaagcgac tcttaggaga cggcacacaa tttggtgtca acatcagcta cgcgacacag gatcatcccg acggtctggc gcaggcattc gtcattggtg ccaatcacat cggcgccgac acagtggcat tggttctggg ggacaacatt ttctatggac ccggactggg gacaagcctg agacgcttcc aatatgtcag cggaggagct attttcgcct attgtgtggc taatccgtcg tcctatggta tcgtagagct gggtattgat ggcatagcgt tatccttaga ggagaaaccc gccacgccaa aatctcagta cgcggttccg ggtttgtatt tctatgacaa cgacgtggtc gagattgcca ggggcttaac taaatcagcg cgaggtgaat atgagatcac ggaggttaat cagatctacc tcaaccaagg ccggctgacg gtcgaagtgt tggctcgcgg gacggcatgg cttgacacgg ggactttcga ctcactgctg gacgctagtg actttgtccg cacgttggaa cgtcggcaag gtttgaaggt cagcgttccc gaggaagtgt cgtggcggat gggttggatc gacgacgagc agctcgcctt gcgcgcacat agcttggcta agtcagggta tggctgttac ctgtcagaac ttttggaacg cggttga

Details for MyleA.00118.a.A1.GE34154

HARVESTED ON:	12/6/2011
SEQUENCED ON:	10/23/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	89

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRGIILAGG SGTRLYPITL GISKQLLPVY DKPMIYYPLS TLMMAGIRDI LVITTAHDAP GFKRLLGDGT QFGVNISYAT QDHPDGLAQA FVIGANHIGA DTVALVLGDN IFYGPGLGTS LRRFQYVSGG AIFAYCVANP SSYGIVELGI DGIALSLEEK PATPKSQYAV PGLYFYDNDV VEIARGLTKS ARGEYEITEV NQIYLNQGRL TVEVLARGTA WLDTGTFDSL LDASDFVRTL ERRXGLKVSV PXEVSWX

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggg ggattatctt ggctggcggc tctggtacac ggctgtaccc aatcacgctg ggcattagca agcagttatt gccggtatac gacaaaccta tgatttatta tccgctgtcc acgctgatga tggccggcat ccgcgacatc ttggtgatta ccaccgctca cgacgcaccc gggtttaagc gactcttagg agacggcaca caatttggtg tcaacatcag ctacgcgaca caggatcatc ccgacggtct ggcgcaggca ttcgtcattg gtgccaatca catcggcgcc gacacagtgg cattggttct gggggacaac attttctatg gacccggact ggggacaagc ctgagacgct tccaatatgt cagcggagga gctattttcg cctattgtgt ggctaatccg tcgtcctatg gtatcgtaga gctgggtatt gatggcatag cgttatcctt agaggagaaa cccgccacgc caaaatctca gtacgcggtt ccgggtttgt atttctatga caacgacgtg gtcgagattg ccaggggctt aactaaatca gcgcgaggtg aatatgagat cacggaggtt aatcagatct acctcaacca aggccggctg acggtcgaag tgttggctcg cgggacggca tggcttgaca cggggacttt cgactcactg ctggacgcta gtgactttgt ccgcacgttg gaacgtcggn aaggtttgaa ggtcagcgtt cccnaggaag tgtcgtggcn na

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgggggatt atcttggctg gcggctctgg tacacggctg tacccaatca cgctgggcat tagcaagcag ttattgccgg tatacgacaa acctatgatt tattatccgc tgtccacgct gatgatggcc ggcatccgcg acatcttggt gattaccacc gctcacgacg cacccgggtt taagcgactc ttaggagacg gcacacaatt tggtgtcaac atcagctacg cgacacagga tcatcccgac ggtctggcgc aggcattcgt cattggtgcc aatcacatcg gcgccgacac agtggcattg gttctggggg acaacatttt ctatggaccc ggactgggga caagcctgag acgcttccaa tatgtcagcg gaggagctat tttcgcctat tgtgtggcta atccgtcgtc ctatggtatc gtagagctgg gtattgatgg catagcgtta tccttagagg agaaacccgc cacgccaaaa tctcagtacg cggttccggg tttgtatttc tatgacaacg acgtggtcga gattgccagg ggcttaacta aatcagcgcg aggtgaatat gagatcacgg aggttaatca gatctacctc aaccaaggcc ggctgacggt cgaagtgttg gctcgcggga cggcatggct tgacacgggg actttcgact cactgctgga cgctagtgac tttgtccgca cgttggaacg tcggcaaggt ttgaaggtca gcgttcccga ggaagtgtcg tggcggatgg gttggatcga cgacgagcag ctcgccttgc gcgcacatag cttggctaag tcagggtatg gctgttacct gtcagaactt ttggaacgcg gttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa