MyleA.00113.a

3-OXOACYL-[ACYL-CARRIER PROTEIN] SYNTHASE 2 KASB (BETA-KETOACYL-ACP SYNTHASE) (KAS I)

CENTER ID: MyleA.00113.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00113.a.A1.GE34153 full length 1 425
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3134
RefSeq: YP_002503767.1
UniProt: A0A0H3MQX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIARLMTTSP ELVTGKAFPN VVVTGIAMTT ALATDAETTW KLLLDNQSGI RMLDDPFIEE FNLPVRIGGH LLEEFDHQLT RVELRRMGYL QRMSTVLSRR LWENAGSPEV DTNRLMVSIG TGLGSAEELV FSYDDMRARG MKAVSPLAVQ KYMPNGAAAA VGLEHHAKAG VMTPVSACAS GSEAIAHAWQ QIVLGEADSA ICGGVETKIE AVPIAGFSQM RIVMSTKNDN PAGACRPFDR DRDGFVFGEA GALMLIETED SAKARSANIL ARIMGASITS DGFHMVAPDP NGERAGHAIA RAVHLAGLSP SDIDHVNAHA TGTQVGDLAE AKAINKALCN NRPAVYAPKS ALGHSVGAVG AVESILTVLA LRDQVIPPTL NLVNLDPDID LDVVAGKPRP GDYRYAVNNS FGFGGHNVAI AFGCY
NT Sequence
atgatcgcta gactcatgac cacatcgccg gagctggtta cagggaaagc ctttcccaat gtggtcgtca ccggcattgc tatgacgact gcgctggcaa cagacgcgga gaccacgtgg aagttgttgc tcgacaacca aagtggaatt cgtatgctcg acgacccgtt catcgaggag ttcaacctac cggttcgcat cggtggacat ttgctggagg aattcgacca ccagctgacc cgcgtcgagc tgcgcaggat gggttaccta cagagaatgt ccaccgtgct aagccggcga ctgtgggaga acgcgggctc acccgaagtg gacaccaatc ggttaatggt gtccatcggc accgggttgg gctcggccga ggagctggtc ttcagctacg acgacatgcg tgcgcgcgga atgaaggcag tctcgccact ggccgtgcag aagtacatgc ccaacggtgc ggcagcggcc gtcgggctgg agcaccacgc caaggctggg gtgatgacgc ctgtgtcggc gtgtgcgtcc ggttccgagg ccatcgccca tgcgtggcag cagatcgtgc tcggcgaagc cgactcggcg atctgtggtg gcgtggaaac aaagatcgag gccgtgccaa tcgccgggtt ctcccagatg cgcatcgtaa tgtccaccaa gaacgacaac cccgccggtg catgtcgccc gttcgatagg gaccgcgacg gcttcgtgtt cggtgaggcc ggcgcgctca tgctaatcga gaccgaggac agtgcgaagg cgcgcagcgc caacatccta gcccggatca tgggggccag catcacctcg gacggcttcc acatggtggc accggacccc aacggggaac gtgccgggca tgctatcgca cgtgcagtgc acctagcggg cctcagtccg agcgacatcg accacgtcaa cgcccatgcc accggcaccc aggtcggtga cctggccgaa gccaaagcca tcaacaaagc cctctgcaac aatcgaccgg cggtgtacgc acccaaatct gccctcggcc attcagtggg cgcagtcggc gcggtggaat caatcttgac tgtgctggcg ttgcgggacc aggtaatccc accgacactg aacctggtaa acctcgatcc agatattgac ttggacgtgg tagcgggtaa accacggccg ggtgattatc ggtatgcggt caacaactcg ttcggattcg gcggtcacaa cgtagcgatc gccttcggct gctactaa
Details for MyleA.00113.a.A1.GE34153
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIARLMTTS PELVTGKAFP NVVVTGIAMT TALATDAETT WKLLLDNQSG IRMLDDPFIE EFNLPVRIGG HLLEEFDHQL TRVELRRMGY LQRMSTVLSR RLWENAGSPE VDTNRLMVSI GTGLGSAEEL VFSYDDMRAR GMKAVSPLAV QKYMPNGAAA AVGLEHHAKA GVMTPVSACA SGSEAIAHAW QQIVLGEADS AICGGVETKI EAVPIAGFSQ MRIVMSTKND NPAGACRPFD RDRDGFVFGE AGALMLIETE DSAKARSANI LARIMGASIX SXGFHMVAXT XXGXCRGYRX CSXLXGXVXX XXRXXXHXXX XXXXXXXXAX XXDXXTXPXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttntttactt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgatcgcta gactcatgac cacatcgccg gagctggtta cagggaaagc ctttcccaat gtggtcgtca ccggcattgc tatgacgact gcgctggcaa cagacgcgga gaccacgtgg aagttgttgc tcgacaacca aagtggaatt cgtatgctcg acgacccgtt catcgaggag ttcaacctac cggttcgcat cggtggacat ttgctggagg aattcgacca ccagctgacc cgcgtcgagc tgcgcaggat gggttaccta cagagaatgt ccaccgtgct aagccggcga ctgtgggaga acgcgggctc acccgaagtg gacaccaatc ggttaatggt gtccatcggc accgggttgg gctcggccga ggagctggtc ttcagctacg acgacatgcg tgcgcgcgga atgaaggcag tctcgccact ggccgtgcag aagtacatgc ccaacggtgc ggcagcggcc gtcgggctgg agcaccacgc caaggctggg gtgatgacgc ctgtgtcggc gtgtgcgtcc ggttccgagg ccatcgccca tgcgtggcag cagatcgtgc tcggcgaagc cgactcggcg atctgtggtg gcgtggaaac aaagatcgag gccgtgccaa tcgccgggtt ctcccagatg cgcatcgtaa tgtccaccaa gaacgacaac cccgccggtg catgtcgccc gttcgatagg gaccgcgacg gcttcgtgtt cggtgaggcc ggcgcgctca tgctaatcga gaccgaggac agtgcgaagg cgcgcagcgc caacatccta gcccggatca tgggggccag catcnnctcn ganggcttcc acatggtggc acnnacccna nggggnangt gccggggcta tcgcangtgc agtgnnctan cgggnncagt nnannnnnnt cnacgtnann ncntgcacng nancnnnnng nnacnnnnan nannntnann agcnnngnan anngacgnnn nnacnnnncc nnnnnncnnn nnnntnnnnn nnnnnnngnn nnantnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnn nnnant
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIARLMTTS PELVTGKAFP NVVVTGIAMT TALATDAETT WKLLLDNQSG IRMLDDPFIE EFNLPVRIGG HLLEEFDHQL TRVELRRMGY LQRMSTVLSR RLWENAGSPE VDTNRLMVSI GTGLGSAEEL VFSYDDMRAR GMKAVSPLAV QKYMPNGAAA AVGLEHHAKA GVMTPVSACA SGSEAIAHAW QQIVLGEADS AICGGVETKI EAVPIAGFSQ MRIVMSTKND NPAGACRPFD RDRDGFVFGE AGALMLIETE DSAKARSANI LARIMGASIT SDGFHMVAPD PNGERAGHAI ARAVHLAGLS PSDIDHVNAH ATGTQVGDLA EAKAINKALC NNRPAVYAPK SALGHSVGAV GAVESILTVL ALRDQVIPPT LNLVNLDPDI DLDVVAGKPR PGDYRYAVNN SFGFGGHNVA IAFGCY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgctaga ctcatgacca catcgccgga gctggttaca gggaaagcct ttcccaatgt ggtcgtcacc ggcattgcta tgacgactgc gctggcaaca gacgcggaga ccacgtggaa gttgttgctc gacaaccaaa gtggaattcg tatgctcgac gacccgttca tcgaggagtt caacctaccg gttcgcatcg gtggacattt gctggaggaa ttcgaccacc agctgacccg cgtcgagctg cgcaggatgg gttacctaca gagaatgtcc accgtgctaa gccggcgact gtgggagaac gcgggctcac ccgaagtgga caccaatcgg ttaatggtgt ccatcggcac cgggttgggc tcggccgagg agctggtctt cagctacgac gacatgcgtg cgcgcggaat gaaggcagtc tcgccactgg ccgtgcagaa gtacatgccc aacggtgcgg cagcggccgt cgggctggag caccacgcca aggctggggt gatgacgcct gtgtcggcgt gtgcgtccgg ttccgaggcc atcgcccatg cgtggcagca gatcgtgctc ggcgaagccg actcggcgat ctgtggtggc gtggaaacaa agatcgaggc cgtgccaatc gccgggttct cccagatgcg catcgtaatg tccaccaaga acgacaaccc cgccggtgca tgtcgcccgt tcgataggga ccgcgacggc ttcgtgttcg gtgaggccgg cgcgctcatg ctaatcgaga ccgaggacag tgcgaaggcg cgcagcgcca acatcctagc ccggatcatg ggggccagca tcacctcgga cggcttccac atggtggcac cggaccccaa cggggaacgt gccgggcatg ctatcgcacg tgcagtgcac ctagcgggcc tcagtccgag cgacatcgac cacgtcaacg cccatgccac cggcacccag gtcggtgacc tggccgaagc caaagccatc aacaaagccc tctgcaacaa tcgaccggcg gtgtacgcac ccaaatctgc cctcggccat tcagtgggcg cagtcggcgc ggtggaatca atcttgactg tgctggcgtt gcgggaccag gtaatcccac cgacactgaa cctggtaaac ctcgatccag atattgactt ggacgtggta gcgggtaaac cacggccggg tgattatcgg tatgcggtca acaactcgtt cggattcggc ggtcacaacg tagcgatcgc cttcggctgc tactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa