MyleA.00097.a

PROBABLE PANTOATE--BETA-ALANINE LIGASE PANC (PANTOTHENATE SYNTHETASE) (PANTOATE ACTIVATING ENZYME)

CENTER ID: MyleA.00097.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00097.a.A1.GE34152 full length 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.359
RefSeq: YP_002502921.1
UniProt: A0A0H3MPC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNSRQPAFN PGELNVYSTP RDVTNVSSAL RHTGRRVMLV PTMGALHNGH LALVRAAKRV PGSVVVVSIF VNPLQFGAAE DLDTYPCTFD DDLALLRAED VEIVFTPTAA AMYPHGLRTT VRPGALAFEL EGGPRPNHFD GVLTVVLKLL QIVRPDRVFF GEKDYQQLVL IRQMVADLNV DVVVVGVPTV REVDGLAMSS RNCYLDPVQR DLAAALSAAL TAGAHAATGG AQAALDAARA VLDATPGLVV DYVELRDAGL GPMRDNTFGR LLVAARLGAT RLLDNISIEI GNFSGIASPD ECRVEHAQTP WMK
NT Sequence
atgacgaaca gtcggcagcc ggcattcaac ccgggcgagc tcaatgtgta ctcgacgccc cgtgacgtca ccaatgtcag cagcgcgttg cgccacaccg gccgccgggt gatgctggtg ccgacgatgg gtgcgctgca caacgggcac ctggccctgg tgcgtgcggc taaacgtgtg cctggttcgg tggttgtggt gtcgatcttc gttaacccgc tgcaattcgg cgccgcggaa gacctcgaca cttacccatg taccttcgac gatgatttgg ctctactgcg cgcagaagat gtcgagatcg tgttcacgcc gactgccgcg gcgatgtatc cgcacggatt gcgtaccacc gtacgccccg gggcgttagc gttcgagctc gaaggtggtc cgcggccaaa ccatttcgac ggtgtgctaa ccgtcgtgct caaactactc cagatcgtgc ggcctgaccg agttttcttc ggggagaaag attaccagca gctggtcctg atccggcaga tggtagccga tctgaacgtc gacgtcgtcg tggtcggcgt cccgaccgtg cgcgaagtcg atgggctggc gatgtcgtca cgcaactgct acctggatcc tgtgcagcgc gatctggccg cagcgctgtc ggcggcgttg acggccgggg cgcacgcagc gacagggggg gcacaggctg cgctggacgc agcccgcgcg gtgctggacg ctacgcccgg ccttgtggtc gattacgtgg agctacgcga tgctgggctc ggcccgatgc gcgacaacac gttcggccgg ttgctagtgg cagccaggct tggtgccact agactactcg acaatatttc gattgagatc ggaaatttct ccggcatcgc tagtccggac gaatgtcggg ttgaacacgc ccaaacacct tggatgaagt ga
Details for MyleA.00097.a.A1.GE34152
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSRQPAF NPGELNVYST PRDVTNVSSA LRHTGRRVML VPTMGALHNG HLALVRAAKR VPGSVVVVSI FVNPLQFGAA EDLDTYPCTF DDDLALLRAE DVEIVFTPTA AAMYPHGLRT TVRPGALAFE LEGGPRPNHF DGVLTVVLKL LQIVRPDRVF FGEKDYQQLV LIRQMVADLN VDVVVVGVPT VREVDGLAMS SRNCYLDPVQ RDLAAALSAA LTAGAHAATG GAQAALDAAR AVLDATPGLV VDYVELRDAG LGPMRDNTFG RLLVAARLGA TRLLDNISIE IGNFSGIASP DECRVEHAQT PWM
Validated NT Sequence
ttcatccaag gtgtttgggc gtgttcaacc cgacattcgt ccggactagc gatgccggag aaatttccga tctcaatcga aatattgtcg agtagtctag tggcaccaag cctggctgcc actagcaacc ggccgaacgt gttgtcgcgc atcgggccga gcccagcatc gcgtagctcc acgtaatcga ccacaaggcc gggcgtagcg tccagcaccg cgcgggctgc gtccagcgca gcctgtgccc cccctgtcgc tgcgtgcgcc ccggccgtca acgccgccga cagcgctgcg gccagatcgc gctgcacagg atccaggtag cagttgcgtg acgacatcgc cagcccatcg acttcgcgca cggtcgggac gccgaccacg acgacgtcga cgttcagatc ggctaccatc tgccggatca ggaccagctg ctggtaatct ttctccccga agaaaactcg gtcaggccgc acgatctgga gtagtttgag cacgacggtt agcacaccgt cgaaatggtt tggccgcgga ccaccttcga gctcgaacgc taacgccccg gggcgtacgg tggtacgcaa tccgtgcgga tacatcgccg cggcagtcgg cgtgaacacg atctcgacat cttctgcgcg cagtagagcc aaatcatcgt cgaaggtaca tgggtaagtg tcgaggtctt ccgcggcgcc gaattgcagc gggttaacga agatcgacac cacaaccacc gaaccaggca cacgtttagc cgcacgcacc agggccaggt gcccgttgtg cagcgcaccc atcgtcggca ccagcatcac ccggcggccg gtgtggcgca acgcgctgct gacattggtg acgtcacggg gcgtcgagta cacattgagc tcgcccgggt tgaatgccgg ctgccgactg ttcgtcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSRQPAF NPGELNVYST PRDVTNVSSA LRHTGRRVML VPTMGALHNG HLALVRAAKR VPGSVVVVSI FVNPLQFGAA EDLDTYPCTF DDDLALLRAE DVEIVFTPTA AAMYPHGLRT TVRPGALAFE LEGGPRPNHF DGVLTVVLKL LQIVRPDRVF FGEKDYQQLV LIRQMVADLN VDVVVVGVPT VREVDGLAMS SRNCYLDPVQ RDLAAALSAA LTAGAHAATG GAQAALDAAR AVLDATPGLV VDYVELRDAG LGPMRDNTFG RLLVAARLGA TRLLDNISIE IGNFSGIASP DECRVEHAQT PWMK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaacagt cggcagccgg cattcaaccc gggcgagctc aatgtgtact cgacgccccg tgacgtcacc aatgtcagca gcgcgttgcg ccacaccggc cgccgggtga tgctggtgcc gacgatgggt gcgctgcaca acgggcacct ggccctggtg cgtgcggcta aacgtgtgcc tggttcggtg gttgtggtgt cgatcttcgt taacccgctg caattcggcg ccgcggaaga cctcgacact tacccatgta ccttcgacga tgatttggct ctactgcgcg cagaagatgt cgagatcgtg ttcacgccga ctgccgcggc gatgtatccg cacggattgc gtaccaccgt acgccccggg gcgttagcgt tcgagctcga aggtggtccg cggccaaacc atttcgacgg tgtgctaacc gtcgtgctca aactactcca gatcgtgcgg cctgaccgag ttttcttcgg ggagaaagat taccagcagc tggtcctgat ccggcagatg gtagccgatc tgaacgtcga cgtcgtcgtg gtcggcgtcc cgaccgtgcg cgaagtcgat gggctggcga tgtcgtcacg caactgctac ctggatcctg tgcagcgcga tctggccgca gcgctgtcgg cggcgttgac ggccggggcg cacgcagcga cagggggggc acaggctgcg ctggacgcag cccgcgcggt gctggacgct acgcccggcc ttgtggtcga ttacgtggag ctacgcgatg ctgggctcgg cccgatgcgc gacaacacgt tcggccggtt gctagtggca gccaggcttg gtgccactag actactcgac aatatttcga ttgagatcgg aaatttctcc ggcatcgcta gtccggacga atgtcgggtt gaacacgccc aaacaccttg gatgaagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa