MyleA.00046.a

PROBABLE ALPHA, ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] OTSA (TREHALOSE-6-PHOSPHATE SYNTHASE) (UDP-GLUCOSE-GLUCOSEPHOSP

CENTER ID: MyleA.00046.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00046.a.A1.GE34149 full length 1 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4323
RefSeq: YP_002504097.1
UniProt: A0A0H3MS14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSRGNHGSK TSSDKHLGDS DFVVVANRLP VDQVRLPDGT AIWKRSPGGL VTALEPLLRQ RRGAWVGWPG VINDNVDLDL TIKSIVQDGL TLYPVRLNTH DVAEYYEGFS NATLWPLYHD VIVKPIYHCE WWERYVDVNR RFAETTSRTA AYGGTVWVQD YQLQLVPKML RIMRPDLTIG FFLHIPFPPV ELFMQIPWRT EIIEGLLGAD LVGFHLTSGA QNFLFLSRHL LGANTSRGLV GVRSRFGEVQ LKSHTVQVGA FPISIDSKEI DQATRDRNVR RRAREIRAEL GNPRKILLGV DRLDYTKGID VRLRAFAELL AEGRAKRDDT VLVQLATPSR ERVESYKILR NDIERQVGHI NGEYGEVGHP VVHYLHRPIP RDELIAFYVA SDVMLVTPLR DGMNLVAKEY VACRNDLGGA LVLSEFTGAA AELRQAYLVN PHDLEGVKDT IEAALNQLAE EARRRMRSLR RQVLAHDVDR WARSFLDALA EAPARDAT
NT Sequence
atgacttcgc ggggtaacca tggctcgaag acctcaagtg acaagcacct cggggattcc gactttgtag tggtcgccaa tcgcctgcca gttgatcagg tgcgacttcc cgatggcact gcgatctgga agcgcagtcc aggcgggttg gtcacagcgc tagagccgct gctgcgtcag cggcgcgggg cctgggtcgg ctggccgggc gtaataaacg acaacgtgga cctcgacttg accatcaagt cgatcgtgca ggatggcctt actctttatc cggtacggct gaacacacat gatgtcgccg agtactacga ggggttctcg aacgccacgc tgtggccgct ataccacgac gtcatcgtaa agcccattta ccactgcgaa tggtgggaac gctacgtcga tgtcaaccgg cgcttcgccg aaacaacgtc acgcacagcc gcctacggtg ggaccgtatg ggtgcaggac taccaactgc aattggtacc gaagatgctg cgcataatgc ggcccgactt gaccatcggt ttcttcttgc acatcccgtt cccgccggtg gagctgttca tgcagatacc atggcgcacc gagatcatcg agggtctgct cggggccgac ctagtgggat tccatctgac cagcggtgcg cagaatttcc tgttcctgtc ccgacatctg ctcggcgcta acacgtcgcg cggattggtt ggtgtgcgat cacggttcgg tgaagtgcaa ttgaagtccc acacagtcca agtgggcgcg tttcccatct cgatcgactc caaagaaatc gaccaagcca cccgcgaccg caacgtccgg cgccgcgccc gcgaaatccg ggctgagcta ggtaaccccc gcaaaatcct gctcggcgtt gaccgactcg actacaccaa gggcatcgat gttcgattga gggccttcgc tgaactgctc gccgagggtc gggcgaagcg cgacgacacc gttctagtcc agctagcgac accgagccgc gaacgagtag aaagctataa gattttacgc aacgacatcg aacgccaagt tggccatatc aacggcgaat acggcgaggt cggtcatccc gtggtgcact acttgcaccg cccaattccc cgcgacgagc tcatcgcgtt ctacgtggca agtgatgtca tgttggtcac cccgctgcgg gacgggatga acctagtggc caaggaatac gtcgcgtgcc gcaacgatct cggcggtgcg ctcgtcctca gcgaattcac cggagcagcc gcggaactcc ggcaggccta tctagtcaac ccacacgacc tagaaggggt taaggacacg atcgaagcgg cgctcaacca gctggccgaa gaagcacggc gccggatgcg gtcgctgcga cgccaagtgc tcgcccatga cgtcgatcgc tgggcgcgat catttctcga cgcgctcgcc gaagcgccgg cgcgcgacgc gacataa
Details for MyleA.00046.a.A1.GE34149
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 58kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 51
Validated AA Sequence
MAHHHXHHMG TLEAQTQGPG SMTSRGNHGS KTSSDKHLGD SDFVVVANRL PVDQVRLPDG TAIWKRSPGG LVTALEPLLR QRRGAWVGWP GVINDNVDLD LTIKSIVQDG LTLYPVRLNT HDVAEYYEGF SNATLWPLYH DVIVKPIYHC EWWERYVDVN RRFAETTSRT AAYGGTVWVQ DYTAIGTEDA AHNAAXLDHR FLLAHXVPAG GAVHADTMXX RDHRGSARGR PSGIPSDQXC XXFPVPVPTS AXRXHXARIX WCAITXR
Validated NT Sequence
ttnnttactt taagaaggag atataccatg gctcatcacc atcnccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgacttcgc ggggtaacca tggctcgaag acctcaagtg acaagcacct cggggattcc gactttgtag tggtcgccaa tcgcctgcca gttgatcagg tgcgacttcc cgatggcact gcgatctgga agcgcagtcc aggcgggttg gtcacagcgc tagagccgct gctgcgtcag cggcgcgggg cctgggtcgg ctggccgggc gtaataaacg acaacgtgga cctcgacttg accatcaagt cgatcgtgca ggatggcctt actctttatc cggtacggct gaacacacat gatgtcgccg agtactacga ggggttctcg aacgccacgc tgtggccgct ataccacgac gtcatcgtaa agcccattta ccactgcgaa tggtgggaac gctacgtcga tgtcaaccgg cgcttcgccg aaacaacgtc acgcacagcc gcctacggtg ggaccgtatg ggtgcaggac tacactgcaa ttggtaccga agatgctgcg cataatgcgg ccngacttga ccatcggttt cttcttgcac atcncgttcc cgccggtgga gctgttcatg cagataccat gncgnaccga gatcatcgag ggtctgctcg gggccgacct agtgggattc catctgacca gngntgcnca naatttcctg ttcctgtccc nacatctgct cngcgctnac acntcgcgcg nattgnntgg tgtgcgatca cngntcggtg aagtgcaatt ganntcccnc acantccang tgggnncgtt tcccatctcg atcgantnna aagaanncga ccaagccnnn ngnnaccgcn acgtnnggcg ccgcnnccnn nnaaatcngg nntnngctag gtaannnccn gcaaatnntg ntcgcntnac nantcgacnn nnccnaggnn nnnnatnnnn nantnagnnn tnnnntnann tgntcnnnnn nggnnnggnc nnnnnnnnnn acnanncnnt nntannnnng ctnncnnnnn nnnnnnnnnn nannnannnt nnnnnannnc nnnannncnt nnannnccna nnnnnnnnnn ncngnnnann gnnnnnnnnn nntncnnngn nnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSRGNHGS KTSSDKHLGD SDFVVVANRL PVDQVRLPDG TAIWKRSPGG LVTALEPLLR QRRGAWVGWP GVINDNVDLD LTIKSIVQDG LTLYPVRLNT HDVAEYYEGF SNATLWPLYH DVIVKPIYHC EWWERYVDVN RRFAETTSRT AAYGGTVWVQ DYQLQLVPKM LRIMRPDLTI GFFLHIPFPP VELFMQIPWR TEIIEGLLGA DLVGFHLTSG AQNFLFLSRH LLGANTSRGL VGVRSRFGEV QLKSHTVQVG AFPISIDSKE IDQATRDRNV RRRAREIRAE LGNPRKILLG VDRLDYTKGI DVRLRAFAEL LAEGRAKRDD TVLVQLATPS RERVESYKIL RNDIERQVGH INGEYGEVGH PVVHYLHRPI PRDELIAFYV ASDVMLVTPL RDGMNLVAKE YVACRNDLGG ALVLSEFTGA AAELRQAYLV NPHDLEGVKD TIEAALNQLA EEARRRMRSL RRQVLAHDVD RWARSFLDAL AEAPARDAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacttcgcgg ggtaaccatg gctcgaagac ctcaagtgac aagcacctcg gggattccga ctttgtagtg gtcgccaatc gcctgccagt tgatcaggtg cgacttcccg atggcactgc gatctggaag cgcagtccag gcgggttggt cacagcgcta gagccgctgc tgcgtcagcg gcgcggggcc tgggtcggct ggccgggcgt aataaacgac aacgtggacc tcgacttgac catcaagtcg atcgtgcagg atggccttac tctttatccg gtacggctga acacacatga tgtcgccgag tactacgagg ggttctcgaa cgccacgctg tggccgctat accacgacgt catcgtaaag cccatttacc actgcgaatg gtgggaacgc tacgtcgatg tcaaccggcg cttcgccgaa acaacgtcac gcacagccgc ctacggtggg accgtatggg tgcaggacta ccaactgcaa ttggtaccga agatgctgcg cataatgcgg cccgacttga ccatcggttt cttcttgcac atcccgttcc cgccggtgga gctgttcatg cagataccat ggcgcaccga gatcatcgag ggtctgctcg gggccgacct agtgggattc catctgacca gcggtgcgca gaatttcctg ttcctgtccc gacatctgct cggcgctaac acgtcgcgcg gattggttgg tgtgcgatca cggttcggtg aagtgcaatt gaagtcccac acagtccaag tgggcgcgtt tcccatctcg atcgactcca aagaaatcga ccaagccacc cgcgaccgca acgtccggcg ccgcgcccgc gaaatccggg ctgagctagg taacccccgc aaaatcctgc tcggcgttga ccgactcgac tacaccaagg gcatcgatgt tcgattgagg gccttcgctg aactgctcgc cgagggtcgg gcgaagcgcg acgacaccgt tctagtccag ctagcgacac cgagccgcga acgagtagaa agctataaga ttttacgcaa cgacatcgaa cgccaagttg gccatatcaa cggcgaatac ggcgaggtcg gtcatcccgt ggtgcactac ttgcaccgcc caattccccg cgacgagctc atcgcgttct acgtggcaag tgatgtcatg ttggtcaccc cgctgcggga cgggatgaac ctagtggcca aggaatacgt cgcgtgccgc aacgatctcg gcggtgcgct cgtcctcagc gaattcaccg gagcagccgc ggaactccgg caggcctatc tagtcaaccc acacgaccta gaaggggtta aggacacgat cgaagcggcg ctcaaccagc tggccgaaga agcacggcgc cggatgcggt cgctgcgacg ccaagtgctc gcccatgacg tcgatcgctg ggcgcgatca tttctcgacg cgctcgccga agcgccggcg cgcgacgcga cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa