SSGCID: Target Page

MyleA.00039.a

PROBABLE METHIONINE AMINOPEPTIDASE MAPA (MAP) (PEPTIDASE M) (MetAP)

CENTER ID:	MyleA.00039.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00039.a.A1.GE34148	full length		1	285

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2986
RefSeq:	YP_002503720.1
UniProt:	A0A0H3MVG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPARTALSPG FLSPTLPVPA WIPRPEYVGK PTAQEGSESW VQTPEVVEKM RVAGRIAAGA LTEAGKAVAP GVTTDELDRI VHEYMVNHGA YPSTLGYKGY PKSCCTSLNE VICHGIPDST VIADGDIVNI DVTAYIDGVH GDTNATFLSG DVSESHRLLV ERTREATMRA INAVKPGRAL SIIGRVIESY ANRFGYNVVR HFTGHGIGTT FHNGLVVLHY DQPFVTTIMQ PGMTFTIEPM INLGTLDYEV WDDGWTVVTK DRKWTAQFEH TLLVTDTGVE ILTVQ

NT Sequence

atgcctgccc gcaccgcgct ttcgcccggc tttttgtccc cgaccctgcc ggtgcccgca tggattccgc gccccgaata cgtcggcaag ccgaccgcac aggagggcag cgagtcttgg gtgcagacgc cggaggtggt cgagaagatg cgtgtcgccg gccggatagc ggctggtgcg ttgactgagg ccggcaaggc agtcgcaccc ggagtgacga ccgacgagct ggatcggatt gtccatgaat acatggtcaa ccacggtgcc tacccgtcaa cgctcggcta caagggctac ccgaagtcgt gctgtacgtc gctcaacgag gtcatctgtc acggcattcc agactcgacg gtgatcgctg acggtgacat cgtcaacatt gatgtcactg cctatatcga tggcgtgcac ggcgacacta atgcgacttt tctgtccggc gatgtctccg aatcccatcg gctactcgtt gagcgaacgc gtgaggcgac gatgcgagcg atcaacgccg tcaaacctgg gcgtgcgttg tccatcattg gtcgggtcat cgagtcctat gcaaaccggt tcgggtacaa cgttgttcgt cacttcactg gtcatggcat cggtaccacg tttcacaatg ggctggttgt tttgcattac gaccagccct ttgtcacgac aatcatgcag ccggggatga cattcaccat cgagccaatg atcaacctgg gaacgctaga ttatgaagtc tgggacgacg gctggacagt ggttaccaag gaccgcaaat ggacagcaca gtttgagcac acgttgttgg tgaccgacac cggcgtcgag atactcaccg tgcagtga

Details for MyleA.00039.a.A1.GE34148

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	33kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	113

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPARTALSP GFLSPTLPVP AWIPRPEYVG KPTAQEGSES WVQTPEVVEK MRVAGRIAAG ALTEAGKAVA PGVTTDELDR IVHEYMVNHG AYPSTLGYKG YPKSCCTSLN EVICHGIPDS TVIADGDIVN IDVTAYIDGV HGDTNATFLS GDVSESHRLL VERTREATMR AINAVKPGRA LSIIGRVIES YANRFGYNVV RHFTGHGIGT TFHNXLVVLH YDQPFVTTIM QPGMTFTIEP MINLGTLDYE VWDDGWTVVT KDRKWTAQFE HTLLVTDXGV XILTXQYXQH EQVLQPAXRX XXCXQXXXXX XXXXXXXXXX XXXXXXX

Validated NT Sequence

attttntttn nctttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgcct gcccgcaccg cgctttcgcc cggctttttg tccccgaccc tgccggtgcc cgcatggatt ccgcgccccg aatacgtcgg caagccgacc gcacaggagg gcagcgagtc ttgggtgcag acgccggagg tggtcgagaa gatgcgtgtc gccggccgga tagcggctgg tgcgttgact gaggccggca aggcagtcgc acccggagtg acgaccgacg agctggatcg gattgtccat gaatacatgg tcaaccacgg tgcctacccg tcaacgctcg gctacaaggg ctacccgaag tcgtgctgta cgtcgctcaa cgaggtcatc tgtcacggca ttccagactc gacggtgatc gctgacggtg acatcgtcaa cattgatgtc actgcctata tcgatggcgt gcacggcgac actaatgcga cttttctgtc cggcgatgtc tccgaatccc atcggctact cgttgagcga acgcgtgagg cgacgatgcg agcgatcaac gccgtcaaac ctgggcgtgc gttgtccatc attggtcggg tcatcgagtc ctatgcaaac cggttcgggt acaacgttgt tcgtcacttc actggtcatg gcatcggtac cacgtttcac aatgngctgg ttgttttgca ttacgaccag ccctttgtca cgacaatcat gcagccgggg atgacattca ccatcgagcc aatgatcaac ctgggaacgc tagattatga agtctgggac gacggctgga cagtggttac caaggaccgc aaatggacag cacagtttga gcacacgttg ttggtgaccg acancggcgt cnagatactc accntgcagt ataancagca cgaacaagtn ctgcagccag ctnctcgang nnccngctgc tancaannnn aannnnctna ntnntnctnc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nanc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPARTALSP GFLSPTLPVP AWIPRPEYVG KPTAQEGSES WVQTPEVVEK MRVAGRIAAG ALTEAGKAVA PGVTTDELDR IVHEYMVNHG AYPSTLGYKG YPKSCCTSLN EVICHGIPDS TVIADGDIVN IDVTAYIDGV HGDTNATFLS GDVSESHRLL VERTREATMR AINAVKPGRA LSIIGRVIES YANRFGYNVV RHFTGHGIGT TFHNGLVVLH YDQPFVTTIM QPGMTFTIEP MINLGTLDYE VWDDGWTVVT KDRKWTAQFE HTLLVTDTGV EILTVQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgcccgc accgcgcttt cgcccggctt tttgtccccg accctgccgg tgcccgcatg gattccgcgc cccgaatacg tcggcaagcc gaccgcacag gagggcagcg agtcttgggt gcagacgccg gaggtggtcg agaagatgcg tgtcgccggc cggatagcgg ctggtgcgtt gactgaggcc ggcaaggcag tcgcacccgg agtgacgacc gacgagctgg atcggattgt ccatgaatac atggtcaacc acggtgccta cccgtcaacg ctcggctaca agggctaccc gaagtcgtgc tgtacgtcgc tcaacgaggt catctgtcac ggcattccag actcgacggt gatcgctgac ggtgacatcg tcaacattga tgtcactgcc tatatcgatg gcgtgcacgg cgacactaat gcgacttttc tgtccggcga tgtctccgaa tcccatcggc tactcgttga gcgaacgcgt gaggcgacga tgcgagcgat caacgccgtc aaacctgggc gtgcgttgtc catcattggt cgggtcatcg agtcctatgc aaaccggttc gggtacaacg ttgttcgtca cttcactggt catggcatcg gtaccacgtt tcacaatggg ctggttgttt tgcattacga ccagcccttt gtcacgacaa tcatgcagcc ggggatgaca ttcaccatcg agccaatgat caacctggga acgctagatt atgaagtctg ggacgacggc tggacagtgg ttaccaagga ccgcaaatgg acagcacagt ttgagcacac gttgttggtg accgacaccg gcgtcgagat actcaccgtg cagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa