SSGCID: Target Page

MyleA.00021.a

PROBABLE D-3-PHOSPHOGLYCERATE DEHYDROGENASE SERA1 (PGDH)

CENTER ID:	MyleA.00021.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00021.a.A1.GE34147	full length		1	528

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00021.a.A1.PW34789	full length		1	528

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127006
PATRIC ID:	fig\|561304.4.peg.3201
RefSeq:	YP_002503793.1
UniProt:	A0A0H3MRC2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLPVVLIAD KLAQSTVAAL GDQVEVRWVD GPDRTKLLAA VPEADALLVR SATTVDAEVL AAAPKLKIVA RAGVGLDNVD VDAATARGVL VVNAPTSNIH SAAEHALALL LAASRQIAEA DASLRAHIWK RSSFSGTEIF GKTVGVVGLG RIGQLVAARI AAFGAHVIAY DPYVAPARAA QLGIELMSFD DLLARADFIS VHLPKTPETA GLIDKEALAK TKPGVIIVNA ARGGLVDEVA LADAVRSGHV RAAGLDVFAT EPCTDSPLFE LSQVVVTPHL GASTAEAQDR AGTDVAESVR LALAGEFVPD AVNVDGGVVN EEVAPWLDLV CKLGVLVAAL SDELPASLSV HVRGELASED VEILRLSALR GLFSTVIEDA VTFVNAPALA AERGVSAEIT TGSESPNHRS VVDVRAVASD GSVVNIAGTL SGPQLVQKIV QVNGRNFDLR AQGMNLVIRY VDQPGALGKI GTLLGAAGVN IQAAQLSEDT EGPGATILLR LDQDVPGDVR SAIVAAVSAN KLEVVNLS

NT Sequence

gtggacctgc ctgttgtgtt aattgccgac aaactcgccc aatcaaccgt ggctgccctg ggagaccaag tcgaggtgcg gtgggtggac ggtccagacc ggacgaagct gttagctgca gtacccgagg ccgacgcgtt gttggtgcgg tcggccacta ctgtcgacgc cgaggtgctg gcagccgctc ctaagctcaa gatcgtcgcc cgtgccgggg tagggctaga caacgttgat gtcgatgccg ccaccgcgcg cggtgtcctg gtagtcaacg ccccaacgtc gaacattcac agcgccgctg agcacgcgtt ggcgctgcta ttggcagctt ctcggcagat cgcggaggcc gacgcctcac tgcgtgcaca catctggaaa cggtcgtcgt tctccggcac cgaaattttc ggcaagaccg tcggcgtggt ggggctgggt cggattgggc agttggttgc cgcacggata gcagcgttcg gggctcacgt tatcgcttac gacccgtatg tggcgccggc acgggccgcg cagcttggta tcgagctgat gtcttttgac gatctcctag cccgggccga ttttatctca gtgcatttgc cgaagacgcc cgagacggcg ggcctgatcg acaaggaggc gctggccaaa accaagcccg gtgtcatcat tgtcaatgcc gcacgcggcg gcttagtgga cgaggtggcg ctagccgatg cggtgcgcag cggacatgtt cgggcggccg gtctagatgt gtttgccacc gaaccgtgca ccgatagccc gctgtttgaa ctatcgcagg tggtggtgac accgcatctg ggggcgtcta ccgccgaagc ccaggatcga gcaggtactg atgtggccga aagcgtgcgg ctggcgctgg cgggggagtt tgtgcctgac gcggtcaacg tggacggggg cgtggtcaac gaagaggtgg ctccctggct ggacttggtg tgcaagcttg gggtgctggt agccgcgtta tccgatgaac tgccggcgtc gttgtcggtg cacgtgcgtg gcgagttggc ttctgaagac gttgaaatat tgcggctttc ggccctacgt gggcttttct cgacggtcat agaggatgct gtgacgttcg tcaacgcacc ggcactggcc gccgaacgag gtgtgtccgc tgaaatcact acgggctcgg agagccccaa ccatcgcagt gtggtcgacg tgcgggcggt cgcctccgac ggctcggtgg tcaacatagc cggtacgttg tctgggccgc aactggtgca gaagatcgtg caggtcaatg gtcgtaactt tgatttgcgt gcgcagggca tgaacttggt gatcaggtat gtcgaccaac ctggcgctct gggcaagatt ggcactttgc tgggcgcggc cggggtgaat atccaagctg ctcagctgtc tgaggacacc gaggggccag gtgcgacgat tctgttgagg ctggatcaag acgtgccggg tgatgtgcgg tcggcgatcg tggcagcggt gagtgccaac aagcttgagg tagtcaatct gtcatga

Details for MyleA.00021.a.A1.GE34147

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/13/2011
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	96

Validated AA Sequence

MVDLPVVLIA DKLAQSTVAA LGDQVEVRWV DGPDRTKLLA AVPEADALLV RSATTVDAEV LAAAPKLKIV ARAGVGLDNV DVDAATARGV LVVNAPTSNI HSAAEHALAL LLAASRQIAE ADASLRAHIW KRSSFSGTEI FGKTVGVVGL GRIGQLVAAR IAAFGAHVIA YDPYVAPARA AQLGIELMSF DDLLARADFI SVHLPKTPET AGLIDKEALA XTKPGVIIVN AARGGLVDEV ALADAVRSGH VRAAGLDVFA TEPCTDSPLF ELSQVVVTPH LGASTAEAQD RAXTDVAXSV RXALAGEFVP DAVNVDGGVV NEEVAPWLDL VCKLGVLVAA LSDELPASLS VHXRGELASE DVEILRLSAL RGLFSTVIED AVTFVNAPAL AAERGVSAEI TTGSESPNHR SVVDVRAVAS DGSVVXXXGX XXGPQXVQKI VQVNGXNXXX XAXXMXLVIR YVDXXGALXK IXTLLGAAGV NIQAXQLXXX TEGPGXXXXL RLDQDVPGDV RSAXVAAVSA NKLEVVNX

Validated NT Sequence

accctggaag ctcagaccca gggtcctggt tcgatggtgg acctgcctgt tgtgttaatt gccgacaaac tcgcccaatc aaccgtggct gccctgggag accaagtcga ggtgcggtgg gtggacggtc cagaccggac gaagctgtta gctgcagtac ccgaggccga cgcgttgttg gtgcggtcgg ccactactgt cgacgccgag gtgctggcag ccgctcctaa gctcaagatc gtcgcccgtg ccggggtagg gctagacaac gttgatgtcg atgccgccac cgcgcgcggt gtcctggtag tcaacgcccc aacgtcgaac attcacagcg ccgctgagca cgcgttggcg ctgctattgg cagcttctcg gcagatcgcg gaggccgacg cctcactgcg tgcacacatc tggaaacggt cgtcgttctc cggcaccgaa attttcggca agaccgtcgg cgtggtgggg ctgggtcgga ttgggcagtt ggttgccgca cggatagcag cgttcggggc tcacgttatc gcttacgacc cgtatgtggc gccggcacgg gccgcgcagc ttggtatcga gctgatgtct tttgacgatc tcctagcccg ggccgatttt atctcagtgc atttgccgaa gacgcccgag acggcgggcc tgatcgacaa ggaggcgctg gccnaaacca agcccggtgt catcattgtc aatgccgcac gcggcggctt agtggacgag gtggcgctng ccgatgcggt gcgcagcgga catgttcggg cggccggtct agatgtgttt gccaccgaac cgtgcaccga tagcccgctg tttgaactat cgcaggtggt ggtgacaccg catctggggg cgtctaccgc ngaagcccag gatcgagcan gtactgatgt ggccganagc gtgcggcngg cgctggcggg ggagtttgtg cctgacgcgg tcaacgtgga cgggggcgtg gtcaacgaag aggtggctcc ctggctggac ttggtgtgca agcttggggt gctggtagcc gcgttatccg atgaactgcc ggcgtcgttg tcggtgcacn tgcgtggcga gttggcttct gaagacgttg aaatattgcg gctttcggcc ctacgtgggc ttttctcgac ggtcatagag gatgctgtga cgttcgtcaa cgcaccggca ctggccgccg aacgaggtgt gtccgctgaa atcactacgg gctcggagag ccccaaccat cgcagtgtgg tcgacgtgcg ggcggtcgcc tccgacggct cggtggtcaa nnnnnncggn nngtngtntg ggccgcaant ggtgcagaag atcgtgcagg tcaatggtng taacttnnat nngngtgcnc annncatgaa nttggtgatc aggtatgtcg accancnngg cgctctggnc aagattgnca ctttgctggg ngcggccggg gtgaatatcc aagctgntca gctgtnnnnn nacaccgagg ggccaggtgn gangantntg ttgaggctgg atcaagacgt gccgggtgat gtgcggtcgg cgatngtggc agcggtgagt gccaacaagc ttgaggtagt caatntgt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVDLPVVLI ADKLAQSTVA ALGDQVEVRW VDGPDRTKLL AAVPEADALL VRSATTVDAE VLAAAPKLKI VARAGVGLDN VDVDAATARG VLVVNAPTSN IHSAAEHALA LLLAASRQIA EADASLRAHI WKRSSFSGTE IFGKTVGVVG LGRIGQLVAA RIAAFGAHVI AYDPYVAPAR AAQLGIELMS FDDLLARADF ISVHLPKTPE TAGLIDKEAL AKTKPGVIIV NAARGGLVDE VALADAVRSG HVRAAGLDVF ATEPCTDSPL FELSQVVVTP HLGASTAEAQ DRAGTDVAES VRLALAGEFV PDAVNVDGGV VNEEVAPWLD LVCKLGVLVA ALSDELPASL SVHVRGELAS EDVEILRLSA LRGLFSTVIE DAVTFVNAPA LAAERGVSAE ITTGSESPNH RSVVDVRAVA SDGSVVNIAG TLSGPQLVQK IVQVNGRNFD LRAQGMNLVI RYVDQPGALG KIGTLLGAAG VNIQAAQLSE DTEGPGATIL LRLDQDVPGD VRSAIVAAVS ANKLEVVNLS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacctg cctgttgtgt taattgccga caaactcgcc caatcaaccg tggctgccct gggagaccaa gtcgaggtgc ggtgggtgga cggtccagac cggacgaagc tgttagctgc agtacccgag gccgacgcgt tgttggtgcg gtcggccact actgtcgacg ccgaggtgct ggcagccgct cctaagctca agatcgtcgc ccgtgccggg gtagggctag acaacgttga tgtcgatgcc gccaccgcgc gcggtgtcct ggtagtcaac gccccaacgt cgaacattca cagcgccgct gagcacgcgt tggcgctgct attggcagct tctcggcaga tcgcggaggc cgacgcctca ctgcgtgcac acatctggaa acggtcgtcg ttctccggca ccgaaatttt cggcaagacc gtcggcgtgg tggggctggg tcggattggg cagttggttg ccgcacggat agcagcgttc ggggctcacg ttatcgctta cgacccgtat gtggcgccgg cacgggccgc gcagcttggt atcgagctga tgtcttttga cgatctccta gcccgggccg attttatctc agtgcatttg ccgaagacgc ccgagacggc gggcctgatc gacaaggagg cgctggccaa aaccaagccc ggtgtcatca ttgtcaatgc cgcacgcggc ggcttagtgg acgaggtggc gctagccgat gcggtgcgca gcggacatgt tcgggcggcc ggtctagatg tgtttgccac cgaaccgtgc accgatagcc cgctgtttga actatcgcag gtggtggtga caccgcatct gggggcgtct accgccgaag cccaggatcg agcaggtact gatgtggccg aaagcgtgcg gctggcgctg gcgggggagt ttgtgcctga cgcggtcaac gtggacgggg gcgtggtcaa cgaagaggtg gctccctggc tggacttggt gtgcaagctt ggggtgctgg tagccgcgtt atccgatgaa ctgccggcgt cgttgtcggt gcacgtgcgt ggcgagttgg cttctgaaga cgttgaaata ttgcggcttt cggccctacg tgggcttttc tcgacggtca tagaggatgc tgtgacgttc gtcaacgcac cggcactggc cgccgaacga ggtgtgtccg ctgaaatcac tacgggctcg gagagcccca accatcgcag tgtggtcgac gtgcgggcgg tcgcctccga cggctcggtg gtcaacatag ccggtacgtt gtctgggccg caactggtgc agaagatcgt gcaggtcaat ggtcgtaact ttgatttgcg tgcgcagggc atgaacttgg tgatcaggta tgtcgaccaa cctggcgctc tgggcaagat tggcactttg ctgggcgcgg ccggggtgaa tatccaagct gctcagctgt ctgaggacac cgaggggcca ggtgcgacga ttctgttgag gctggatcaa gacgtgccgg gtgatgtgcg gtcggcgatc gtggcagcgg tgagtgccaa caagcttgag gtagtcaatc tgtcataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for MyleA.00021.a.A1.PW34789

PURIFICATION DATe:	2/17/2012
CONCENTRATION:	29.44mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	93
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGACCTG CCTGTTGTGT TAATTGCCGA CAAACTCGCC CAATCAACCG TGGCTGCCCT GGGAGACCAA GTCGAGGTGC GGTGGGTGGA CGGTCCAGAC CGGACGAAGC TGTTAGCTGC AGTACCCGAG GCCGACGCGT TGTTGGTGCG GTCGGCCACT ACTGTCGACG CCGAGGTGCT GGCAGCCGCT CCTAAGCTCA AGATCGTCGC CCGTGCCGGG GTAGGGCTAG ACAACGTTGA TGTCGATGCC GCCACCGCGC GCGGTGTCCT GGTAGTCAAC GCCCCAACGT CGAACATTCA CAGCGCCGCT GAGCACGCGT TGGCGCTGCT ATTGGCAGCT TCTCGGCAGA TCGCGGAGGC CGACGCCTCA CTGCGTGCAC ACATCTGGAA ACGGTCGTCG TTCTCCGGCA CCGAAATTTT CGGCAAGACC GTCGGCGTGG TGGGGCTGGG TCGGATTGGG CAGTTGGTTG CCGCACGGAT AGCAGCGTTC GGGGCTCACG TTATCGCTTA CGACCCGTAT GTGGCGCCGG CACGGGCCGC GCAGCTTGGT ATCGAGCTGA TGTCTTTTGA CGATCTCCTA GCCCGGGCCG ATTTTATCTC AGTGCATTTG CCGAAGACGC CCGAGACGGC GGGCCTGATC GACAAGGAGG CGCTGGCCAA AACCAAGCCC GGTGTCATCA TTGTCAATGC CGCACGCGGC GGCTTAGTGG ACGAGGTGGC GCTAGCCGAT GCGGTGCGCA GCGGACATGT TCGGGCGGCC GGTCTAGATG TGTTTGCCAC CGAACCGTGC ACCGATAGCC CGCTGTTTGA ACTATCGCAG GTGGTGGTGA CACCGCATCT GGGGGCGTCT ACCGCCGAAG CCCAGGATCG AGCAGGTACT GATGTGGCCG AAAGCGTGCG GCTGGCGCTG GCGGGGGAGT TTGTGCCTGA CGCGGTCAAC GTGGACGGGG GCGTGGTCAA CGAAGAGGTG GCTCCCTGGC TGGACTTGGT GTGCAAGCTT GGGGTGCTGG TAGCCGCGTT ATCCGATGAA CTGCCGGCGT CGTTGTCGGT GCACGTGCGT GGCGAGTTGG CTTCTGAAGA CGTTGAAATA TTGCGGCTTT CGGCCCTACG TGGGCTTTTC TCGACGGTCA TAGAGGATGC TGTGACGTTC GTCAACGCAC CGGCACTGGC CGCCGAACGA GGTGTGTCCG CTGAAATCAC TACGGGCTCG GAGAGCCCCA ACCATCGCAG TGTGGTCGAC GTGCGGGCGG TCGCCTCCGA CGGCTCGGTG GTCAACATAG CCGGTACGTT GTCTGGGCCG CAACTGGTGC AGAAGATCGT GCAGGTCAAT GGTCGTAACT TTGATTTGCG TGCGCAGGGC ATGAACTTGG TGATCAGGTA TGTCGACCAA CCTGGCGCTC TGGGCAAGAT TGGCACTTTG CTGGGCGCGG CCGGGGTGAA TATCCAAGCT GCTCAGCTGT CTGAGGACAC CGAGGGGCCA GGTGCGACGA TTCTGTTGAG GCTGGATCAA GACGTGCCGG GTGATGTGCG GTCGGCGATC GTGGCAGCGG TGAGTGCCAA CAAGCTTGAG GTAGTCAATC TGTCATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA