MykaA.00472.a
Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) (InhA)
| CENTER ID: | MykaA.00472.a |
| ORGANISM: | Mycobacterium kansasii |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MykaA.00472.a.B1.GE42833 | full length | 1 | 269 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MykaA.00472.a.B1.PS38576 | full length | 1 | 269 |
Structures
6UDF
DEPOSITED: 9/19/2019
DETERMINATION: XRay
CLONE: MykaA.00472.a.B1.GE42833
PROTEIN: MykaA.00472.a.B1.PS38576
7KXA
DEPOSITED: 12/3/2020
DETERMINATION: XRay
CLONE: MykaA.00472.a.B1.GE42833
PROTEIN: MykaA.00472.a.B1.PS38576
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| UniProt: | C3VPN3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGLLDGKRI LVSGIITDSS IAFHIARVAQ EQGAQLVLTG FDRLRLIQRI TDRLPEKAPL LELDVQNDEH LATLADRVVE VIGEGNKLDG LVHSIGFMPQ SGMGINPFFD APYEDVSKGI HISAYSYASM AKALLPIMNR GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG KYGVRSNLVA AGPIRTLAMA AIVGGALGEE AGAQIQLLEE GWDQRAPIGW NMKDPTPVAK TVCALLSDRL PATTGDIIFA DGGAHTQLL
NT Sequence
atggcaggac tgctggacgg taaacggatt ctggtgtccg gaatcatcac cgactcctcg atcgcgtttc atatcgcccg ggtggcccag gagcagggcg cacagctggt gctgaccggt ttcgaccggt tgcggctgat ccagcggatc accgaccggt taccggagaa ggcgccgctg ctggagctcg acgtgcaaaa cgacgagcac ctggccaccc tcgcagaccg ggtcgtcgag gtgatcggcg aaggcaacaa actcgacggg ctggtccact cgatcggctt catgccgcaa agcgggatgg ggatcaaccc gttcttcgac gcgccctacg aggacgtgtc caagggcatt cacatctccg cctactccta cgcatccatg gcaaaggcgt tgctgcccat catgaatcgt ggtggctcca tcgtcggcat ggacttcgac ccgagccggg cgatgccggc ctataactgg atgacggtcg ccaagagcgc gctggagtcg gtcaaccggt tcgtggcccg cgaggccggc aagtacggtg tgcgctcgaa cctcgttgcc gccgggccga tccggacgct ggcgatggcg gcaatcgtcg gcggtgcgct gggcgaggag gccggcgccc agatccagct actcgaagag ggctgggacc agcgcgcgcc cattggctgg aacatgaagg acccgacgcc cgtcgccaag accgtctgcg cactgctgtc ggataggctg ccggccacca cgggggacat catcttcgcc gacggcggcg cccacactca gttgctctag
Details for MykaA.00472.a.B1.GE42833
| HARVESTED ON: | 1/9/2019 |
| SEQUENCED ON: | 1/15/2019 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA GLLEGKRILV SGIITDSSIA FHIARVAQEQ GAQLVLTGFD RLRLIQRITD RLPEKAPLLE LDVQNDEHLA TLADRVVEVI GEGNKLDGLV HSIGFMPQSG MGINPFFDAP YEDVSKGIHI SAYSYASMAK ALLPIMNRGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGKY GVRSNLVAAG PIRTLAMAAI VGGALGEEAG AQIQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSEWLPA TTGDIIFAXX XXHTQLL
Validated NT Sequence
cacttagagc aactgagtgt gggnnnnnnn nncggcgaag atgatgtccc ccgtggtggc cggcagccac tccgacagca gtgcgcagac ggtcttggcg acgggcgtcg ggtccttcat gttccagcca atgggcgcgc gctggtccca gccctcttcg agtagctgga tctgggcgcc ggcctcctcg cccagcgcac cgccgacgat tgccgccatc gccagcgtcc ggatcggccc ggcggcaacg aggttcgagc gcacaccgta cttgccggcc tcgcgggcca cgaaccggtt gaccgactcc agcgcgctct tggcgaccgt catccagtta taggccggca tcgcccggct cgggtcgaag tccatgccga cgatggagcc accacgattc atgatgggca gcaacgcctt tgccatggat gcgtaggagt aggcggagat gtgaatgccc ttggacacgt cctcgtaggg cgcgtcgaag aacgggttga tccccatccc gctttgcggc atgaagccga tcgagtggac cagcccgtcg agtttgttgc cttcgccgat cacctcgacg acccggtctg cgagggtggc caggtgctcg tcgttttgca cgtcgagctc cagcagcggc gccttctccg gtaaccggtc ggtgatccgc tggatcagcc gcaaccggtc gaaaccggtc agcaccagct gtgcgccctg ctcctgggcc acccgggcga tatgaaacgc gatcgaggag tcggtgatga tcccggacac caggatccgt ttaccctcca gcagtcctgc catatggtgg tggtggtggt gagccatgg
Expected Protein Sequence
MAHHHHHHMA GLLDGKRILV SGIITDSSIA FHIARVAQEQ GAQLVLTGFD RLRLIQRITD RLPEKAPLLE LDVQNDEHLA TLADRVVEVI GEGNKLDGLV HSIGFMPQSG MGINPFFDAP YEDVSKGIHI SAYSYASMAK ALLPIMNRGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGKY GVRSNLVAAG PIRTLAMAAI VGGALGEEAG AQIQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDRLPA TTGDIIFADG GAHTQLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag gactgctgga cggtaaacgg attctggtgt ccggaatcat caccgactcc tcgatcgcgt ttcatatcgc ccgggtggcc caggagcagg gcgcacagct ggtgctgacc ggtttcgacc ggttgcggct gatccagcgg atcaccgacc ggttaccgga gaaggcgccg ctgctggagc tcgacgtgca aaacgacgag cacctggcca ccctcgcaga ccgggtcgtc gaggtgatcg gcgaaggcaa caaactcgac gggctggtcc actcgatcgg cttcatgccg caaagcggga tggggatcaa cccgttcttc gacgcgccct acgaggacgt gtccaagggc attcacatct ccgcctactc ctacgcatcc atggcaaagg cgttgctgcc catcatgaat cgtggtggct ccatcgtcgg catggacttc gacccgagcc gggcgatgcc ggcctataac tggatgacgg tcgccaagag cgcgctggag tcggtcaacc ggttcgtggc ccgcgaggcc ggcaagtacg gtgtgcgctc gaacctcgtt gccgccgggc cgatccggac gctggcgatg gcggcaatcg tcggcggtgc gctgggcgag gaggccggcg cccagatcca gctactcgaa gagggctggg accagcgcgc gcccattggc tggaacatga aggacccgac gcccgtcgcc aagaccgtct gcgcactgct gtcggatagg ctgccggcca ccacggggga catcatcttc gccgacggcg gcgcccacac tcagttgctc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MykaA.00472.a.B1.PS38576
| PURIFICATION DATe: | 5/7/2019 |
| CONCENTRATION: | 27.08mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA GLLEGKRILV SGIITDSSIA FHIARVAQEQ GAQLVLTGFD RLRLIQRITD RLPEKAPLLE LDVQNDEHLA TLADRVVEVI GEGNKLDGLV HSIGFMPQSG MGINPFFDAP YEDVSKGIHI SAYSYASMAK ALLPIMNRGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGKY GVRSNLVAAG PIRTLAMAAI VGGALGEEAG AQIQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSEWLPA TTGDIIFAXX XXHTQLL
Validated NT Sequence
cacttagagc aactgagtgt gggnnnnnnn nncggcgaag atgatgtccc ccgtggtggc cggcagccac tccgacagca gtgcgcagac ggtcttggcg acgggcgtcg ggtccttcat gttccagcca atgggcgcgc gctggtccca gccctcttcg agtagctgga tctgggcgcc ggcctcctcg cccagcgcac cgccgacgat tgccgccatc gccagcgtcc ggatcggccc ggcggcaacg aggttcgagc gcacaccgta cttgccggcc tcgcgggcca cgaaccggtt gaccgactcc agcgcgctct tggcgaccgt catccagtta taggccggca tcgcccggct cgggtcgaag tccatgccga cgatggagcc accacgattc atgatgggca gcaacgcctt tgccatggat gcgtaggagt aggcggagat gtgaatgccc ttggacacgt cctcgtaggg cgcgtcgaag aacgggttga tccccatccc gctttgcggc atgaagccga tcgagtggac cagcccgtcg agtttgttgc cttcgccgat cacctcgacg acccggtctg cgagggtggc caggtgctcg tcgttttgca cgtcgagctc cagcagcggc gccttctccg gtaaccggtc ggtgatccgc tggatcagcc gcaaccggtc gaaaccggtc agcaccagct gtgcgccctg ctcctgggcc acccgggcga tatgaaacgc gatcgaggag tcggtgatga tcccggacac caggatccgt ttaccctcca gcagtcctgc catatggtgg tggtggtggt gagccatgg
Expressed Protein Sequence
MAHHHHHHMA GLLDGKRILV SGIITDSSIA FHIARVAQEQ GAQLVLTGFD RLRLIQRITD RLPEKAPLLE LDVQNDEHLA TLADRVVEVI GEGNKLDGLV HSIGFMPQSG MGINPFFDAP YEDVSKGIHI SAYSYASMAK ALLPIMNRGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGKY GVRSNLVAAG PIRTLAMAAI VGGALGEEAG AQIQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDRLPA TTGDIIFADG GAHTQLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG GACTGCTGGA CGGTAAACGG ATTCTGGTGT CCGGAATCAT CACCGACTCC TCGATCGCGT TTCATATCGC CCGGGTGGCC CAGGAGCAGG GCGCACAGCT GGTGCTGACC GGTTTCGACC GGTTGCGGCT GATCCAGCGG ATCACCGACC GGTTACCGGA GAAGGCGCCG CTGCTGGAGC TCGACGTGCA AAACGACGAG CACCTGGCCA CCCTCGCAGA CCGGGTCGTC GAGGTGATCG GCGAAGGCAA CAAACTCGAC GGGCTGGTCC ACTCGATCGG CTTCATGCCG CAAAGCGGGA TGGGGATCAA CCCGTTCTTC GACGCGCCCT ACGAGGACGT GTCCAAGGGC ATTCACATCT CCGCCTACTC CTACGCATCC ATGGCAAAGG CGTTGCTGCC CATCATGAAT CGTGGTGGCT CCATCGTCGG CATGGACTTC GACCCGAGCC GGGCGATGCC GGCCTATAAC TGGATGACGG TCGCCAAGAG CGCGCTGGAG TCGGTCAACC GGTTCGTGGC CCGCGAGGCC GGCAAGTACG GTGTGCGCTC GAACCTCGTT GCCGCCGGGC CGATCCGGAC GCTGGCGATG GCGGCAATCG TCGGCGGTGC GCTGGGCGAG GAGGCCGGCG CCCAGATCCA GCTACTCGAA GAGGGCTGGG ACCAGCGCGC GCCCATTGGC TGGAACATGA AGGACCCGAC GCCCGTCGCC AAGACCGTCT GCGCACTGCT GTCGGATAGG CTGCCGGCCA CCACGGGGGA CATCATCTTC GCCGACGGCG GCGCCCACAC TCAGTTGCTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT