MygeA.20193.a

Chaperone protein DnaK (MG_305)

CENTER ID: MygeA.20193.a
ORGANISM: Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MygeA.20193.a.B1.GE42356 Full length(MygeA.20193.a) 1 595
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MygeA.20193.a.B1.PW38483 Full length(MygeA.20193.a) 1 595
Molecular models
METHOD RESULTS
comparative modelling Robetta_81220
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|243273.25.peg.356
UniProt: P47547
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSADNGLIIG IDLGTTNSCV SVMEGGRPVV LENPEGKRTT PSIVSYKNNE IIVGDAAKRQ MVTNPNTIVS IKRLMGTSNK VKVQNADGTT KELSPEQVSA QILSYLKDFA EKKIGKKISR AVITVPAYFN DAERNATKTA GKIAGLNVER IINEPTAAAL AYGIDKASRE MKVLVYDLGG GTFDVSLLDI AEGTFEVLAT AGDNRLGGDD WDNKIIEYIS AYIAKEHQGL NLSKDKMAMQ RLKEAAERAK IELSAQLETI ISLPFLTVTQ KGPVNVELKL TRAKFEELTK PLLERTRNPI SDVIKEAKIK PEEINEILLV GGSTRMPAVQ KLVESMVPGK KPNRSINPDE VVAIGAAIQG GVLRGDVKDV LLLDVTPLTL SIETLGGVAT PLIKRNTTIP VSKSQIFSTA QDNQESVDVV VCQGERPMSR DNKSLGRFNL GGIQPAPKGK PQIEITFSLD ANGILNVKAK DLTTQKENSI TISDNGNLSE EEIQKMIRDA EANKERDNII RERIELRNEG EGIVNTIKEI LASPDAKNFP KEEKEKLEKL TGNIDAAIKA NDYAKLKVEI ENFKKWREEM AKKYNPTGEQ GPQAK
NT Sequence
ATGAGTGCAG ACAATGGTTT AATTATTGGC ATTGACCTTG GAACTACCAA TTCTTGTGTT TCTGTAATGG AAGGTGGGAG ACCTGTTGTA TTAGAAAATC CTGAAGGTAA AAGAACAACA CCTTCCATTG TTTCCTATAA AAACAATGAA ATTATAGTAG GTGATGCTGC TAAAAGACAG ATGGTTACAA ACCCAAATAC CATTGTCTCC ATCAAGAGGT TGATGGGTAC CTCAAATAAA GTAAAAGTCC AAAATGCTGA TGGTACAACT AAGGAATTAA GTCCTGAACA AGTTTCAGCG CAAATCCTTA GTTATCTTAA GGACTTTGCT GAAAAAAAGA TTGGTAAAAA GATTTCAAGA GCAGTTATTA CTGTTCCTGC ATACTTTAAT GATGCGGAAA GAAACGCTAC TAAAACCGCA GGTAAGATTG CTGGTTTAAA TGTTGAAAGG ATCATTAACG AACCAACTGC TGCTGCTTTA GCTTATGGGA TTGATAAAGC ATCAAGAGAG ATGAAAGTCT TGGTTTATGA CTTGGGTGGT GGAACTTTTG ATGTATCTTT ACTTGACATT GCAGAAGGTA CTTTTGAAGT ACTTGCAACT GCTGGGGACA ACCGTTTGGG AGGTGATGAT TGGGATAACA AGATCATTGA ATATATCTCA GCCTACATTG CCAAAGAACA CCAGGGTTTA AACTTATCAA AAGATAAGAT GGCAATGCAA CGGCTTAAAG AAGCAGCTGA ACGTGCTAAG ATTGAACTTT CCGCTCAACT TGAAACGATT ATTTCTCTAC CATTTTTAAC TGTTACCCAA AAAGGTCCTG TTAACGTTGA GTTAAAACTA ACCCGTGCTA AGTTTGAGGA GTTAACAAAA CCACTACTTG AAAGAACAAG AAACCCTATT TCAGATGTTA TCAAGGAAGC TAAGATTAAA CCTGAAGAGA TTAATGAAAT TCTTTTAGTT GGTGGTTCTA CAAGGATGCC TGCAGTTCAA AAGCTAGTTG AATCAATGGT ACCAGGTAAA AAACCAAACC GTTCTATTAA TCCTGATGAA GTTGTTGCTA TTGGCGCTGC TATTCAAGGT GGGGTTTTAC GTGGTGATGT TAAGGATGTT TTACTTTTAG ATGTAACTCC ATTAACCCTT TCTATTGAAA CTTTAGGTGG TGTGGCTACT CCTTTAATTA AGAGAAATAC AACTATCCCA GTAAGTAAAA GTCAAATCTT TTCAACTGCT CAAGATAACC AAGAATCAGT TGATGTGGTT GTATGTCAAG GGGAAAGACC AATGTCTAGA GATAATAAGT CATTAGGAAG ATTTAACTTA GGTGGTATTC AACCAGCACC TAAAGGTAAA CCCCAAATTG AGATTACCTT TAGTTTGGAT GCCAATGGGA TCTTAAATGT TAAAGCTAAG GATTTAACCA CGCAAAAGGA AAACAGTATT ACCATTAGTG ACAACGGTAA TCTTTCTGAG GAGGAGATCC AAAAGATGAT CCGTGATGCT GAAGCTAACA AGGAACGGGA TAACATCATC CGTGAACGTA TTGAATTACG TAATGAAGGG GAAGGTATTG TTAATACCAT CAAAGAGATA TTAGCAAGTC CTGATGCTAA GAATTTCCCT AAAGAAGAAA AAGAGAAGTT AGAAAAGCTA ACAGGTAACA TTGATGCTGC TATTAAAGCT AATGACTATG CCAAACTCAA AGTGGAAATT GAAAACTTTA AGAAGTGAAG AGAAGAGATG GCAAAAAAAT ATAACCCAAC TGGTGAACAA GGTCCACAAG CAAAA
Details for MygeA.20193.a.B1.GE42356
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 91
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMS ADNGLIIGID LGTTNSCVSV MEGGRPVVLE NPEGKRTTPS IVSYKNNEII VGDAAKRQMV TNPNTIVSIK RLMGTSNKVK VQNADGTTKE LSPEQVSAQI LSYLKDFAEK KIGKKISRAV ITVPAYFNDA ERNATKTAGK IAGLNVERII NEPTAAALAY GIDKASREMK VLVYDLGGGT FDVSLLDIAE GTFEVLATAG DNRLGGDDWD NKIIEYISAY IAKEHQGLNL SXXXXAMQRL KEAAXXAXXX XXAQLXXXXS LPXXTXXXXX XVNXXXXXXX XKFEELTKPL XXXXXNPISX XXKEAKIKPE EINEXXXVGX XXRMXAVQKL VESMXXGKKP NRSINPDEVV AIGAAIQGGV LRGDVKDVLL LDVTPLTLSI ETLGGVATPL IKRNTTIPVS KSQIFSTAQD NQESVDVVVC QGERPMSRDN KSLGRFNLGG IQPAPKGKPQ IEITFSLDAN GILNVKAKDL TTQKENSITI SDNGNLSEEE IQKMIRDAEA NKERDNIIRE RIELRNEGEG IVNTIKEILA SPDAKNFPKE EKEKLEKLTG NIDAAIKAND YAKLKVEIEN FKK
Validated NT Sequence
agttgggtta tatttttttg ccatctcttc tcttcacttc ttaaagtttt caatttccac tttgagtttg gcatagtcat tagctttaat agcagcatca atgttacctg ttagcttttc taacttctct ttttcttctt tagggaaatt cttagcatca ggacttgcta atatctcttt gatggtatta acaatacctt ccccttcatt acgtaattca atacgttcac ggatgatgtt atcccgttcc ttgttagctt cagcatcacg gatcatcttt tggatctcct cctcagaaag attaccgttg tcactaatgg taatactgtt ttccttttgc gtggttaaat ccttagcttt aacatttaag atcccattgg catccaaact aaaggtaatc tcaatttggg gtttaccttt aggtgctggt tgaataccac ctaagttaaa tcttcctaat gacttattat ctctagacat tggtctttcc ccttgacata caaccacatc aactgattct tggttatctt gagcagttga aaagatttga cttttactta ctgggatagt tgtatttctc ttaattaaag gagtagccac accacctaaa gtttcaatag aaagggttaa tggagttaca tctaaaagta aaacatcctt aacatcacca cgtaaaaccc caccttgaat agcagcgcca atagcaacaa cttcatcagg attaatagaa cggtttggtt ttttacctnn nnccattgat tcaactagct tttgaactgc anncatcctt ntnnnnncac caactnnnnn natttcatta atctcttcag gtttaatctt ngcttccttg nnnnnntctg aaatagggtt nnnnnnnnnn nnnnntagtg gttttgttaa ctcctcaaac ttnnnnnngn nnnnnnnnnn nnnnncgtta acannnnnnn nnnnggnnnn ngtnnnnaat ggtagagaan nnnncntntc aagttgagcn nnnnnnncnn nnnnngcacn nnnagctgct tctttaagcc gttgcattgc canctnnncn tttgataagt ttaaaccctg gtgttctttg gcaatgtagg ctgagatata ttcaatgatc ttgttatccc aatcatcacc tcccaaacgg ttgtccccag cagttgcaag tacttcaaaa gtaccttctg caatgtcaag taaagataca tcaaaagttc caccacccaa gtcataaacc aagactttca tctctcttga tgctttatca atcccataag ctaaagcagc agcagttggt tcgttaatga tcctttcaac atttaaacca gcaatcttac ctgcggtttt agtagcgttt ctttccgcat cattaaagta tgcaggaaca gtaataactg ctcttgaaat ctttttacca atcttttttt cagcaaagtc cttaagataa ctaaggattt gcgctgaaac ttgttcagga cttaattcct tagttgtacc atcagcattt tggactttta ctttatttga ggtacccatc aacctcttga tggagacaat ggtatttggg tttgtaacca tctgtctttt agcagcatca cctactataa tttcattgtt tttataggaa acaatggaag gtgttgttct tttaccttca ggattttcta atacaacagg tctcccacct tccattacag aaacacaaga attggtagtt ccaaggtcaa tgccaataat taaaccattg tctgcactca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS ADNGLIIGID LGTTNSCVSV MEGGRPVVLE NPEGKRTTPS IVSYKNNEII VGDAAKRQMV TNPNTIVSIK RLMGTSNKVK VQNADGTTKE LSPEQVSAQI LSYLKDFAEK KIGKKISRAV ITVPAYFNDA ERNATKTAGK IAGLNVERII NEPTAAALAY GIDKASREMK VLVYDLGGGT FDVSLLDIAE GTFEVLATAG DNRLGGDDWD NKIIEYISAY IAKEHQGLNL SKDKMAMQRL KEAAERAKIE LSAQLETIIS LPFLTVTQKG PVNVELKLTR AKFEELTKPL LERTRNPISD VIKEAKIKPE EINEILLVGG STRMPAVQKL VESMVPGKKP NRSINPDEVV AIGAAIQGGV LRGDVKDVLL LDVTPLTLSI ETLGGVATPL IKRNTTIPVS KSQIFSTAQD NQESVDVVVC QGERPMSRDN KSLGRFNLGG IQPAPKGKPQ IEITFSLDAN GILNVKAKDL TTQKENSITI SDNGNLSEEE IQKMIRDAEA NKERDNIIRE RIELRNEGEG IVNTIKEILA SPDAKNFPKE EKEKLEKLTG NIDAAIKAND YAKLKVEIEN FKKWREEMAK KYNPTGEQGP QAK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg cagacaatgg tttaattatt ggcattgacc ttggaactac caattcttgt gtttctgtaa tggaaggtgg gagacctgtt gtattagaaa atcctgaagg taaaagaaca acaccttcca ttgtttccta taaaaacaat gaaattatag taggtgatgc tgctaaaaga cagatggtta caaacccaaa taccattgtc tccatcaaga ggttgatggg tacctcaaat aaagtaaaag tccaaaatgc tgatggtaca actaaggaat taagtcctga acaagtttca gcgcaaatcc ttagttatct taaggacttt gctgaaaaaa agattggtaa aaagatttca agagcagtta ttactgttcc tgcatacttt aatgatgcgg aaagaaacgc tactaaaacc gcaggtaaga ttgctggttt aaatgttgaa aggatcatta acgaaccaac tgctgctgct ttagcttatg ggattgataa agcatcaaga gagatgaaag tcttggttta tgacttgggt ggtggaactt ttgatgtatc tttacttgac attgcagaag gtacttttga agtacttgca actgctgggg acaaccgttt gggaggtgat gattgggata acaagatcat tgaatatatc tcagcctaca ttgccaaaga acaccagggt ttaaacttat caaaagataa gatggcaatg caacggctta aagaagcagc tgaacgtgct aagattgaac tttccgctca acttgaaacg attatttctc taccattttt aactgttacc caaaaaggtc ctgttaacgt tgagttaaaa ctaacccgtg ctaagtttga ggagttaaca aaaccactac ttgaaagaac aagaaaccct atttcagatg ttatcaagga agctaagatt aaacctgaag agattaatga aattctttta gttggtggtt ctacaaggat gcctgcagtt caaaagctag ttgaatcaat ggtaccaggt aaaaaaccaa accgttctat taatcctgat gaagttgttg ctattggcgc tgctattcaa ggtggggttt tacgtggtga tgttaaggat gttttacttt tagatgtaac tccattaacc ctttctattg aaactttagg tggtgtggct actcctttaa ttaagagaaa tacaactatc ccagtaagta aaagtcaaat cttttcaact gctcaagata accaagaatc agttgatgtg gttgtatgtc aaggggaaag accaatgtct agagataata agtcattagg aagatttaac ttaggtggta ttcaaccagc acctaaaggt aaaccccaaa ttgagattac ctttagtttg gatgccaatg ggatcttaaa tgttaaagct aaggatttaa ccacgcaaaa ggaaaacagt attaccatta gtgacaacgg taatctttct gaggaggaga tccaaaagat gatccgtgat gctgaagcta acaaggaacg ggataacatc atccgtgaac gtattgaatt acgtaatgaa ggggaaggta ttgttaatac catcaaagag atattagcaa gtcctgatgc taagaatttc cctaaagaag aaaaagagaa gttagaaaag ctaacaggta acattgatgc tgctattaaa gctaatgact atgccaaact caaagtggaa attgaaaact ttaagaagtg aagagaagag atggcaaaaa aatataaccc aactggtgaa caaggtccac aagcaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MygeA.20193.a.B1.PW38483
PURIFICATION DATe: 5/29/2018
CONCENTRATION: 10.84mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 29
VIAL VOLUME: 200µl
PERCENT IDENTITY: 91
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ADNGLIIGID LGTTNSCVSV MEGGRPVVLE NPEGKRTTPS IVSYKNNEII VGDAAKRQMV TNPNTIVSIK RLMGTSNKVK VQNADGTTKE LSPEQVSAQI LSYLKDFAEK KIGKKISRAV ITVPAYFNDA ERNATKTAGK IAGLNVERII NEPTAAALAY GIDKASREMK VLVYDLGGGT FDVSLLDIAE GTFEVLATAG DNRLGGDDWD NKIIEYISAY IAKEHQGLNL SXXXXAMQRL KEAAXXAXXX XXAQLXXXXS LPXXTXXXXX XVNXXXXXXX XKFEELTKPL XXXXXNPISX XXKEAKIKPE EINEXXXVGX XXRMXAVQKL VESMXXGKKP NRSINPDEVV AIGAAIQGGV LRGDVKDVLL LDVTPLTLSI ETLGGVATPL IKRNTTIPVS KSQIFSTAQD NQESVDVVVC QGERPMSRDN KSLGRFNLGG IQPAPKGKPQ IEITFSLDAN GILNVKAKDL TTQKENSITI SDNGNLSEEE IQKMIRDAEA NKERDNIIRE RIELRNEGEG IVNTIKEILA SPDAKNFPKE EKEKLEKLTG NIDAAIKAND YAKLKVEIEN FKK
Validated NT Sequence
agttgggtta tatttttttg ccatctcttc tcttcacttc ttaaagtttt caatttccac tttgagtttg gcatagtcat tagctttaat agcagcatca atgttacctg ttagcttttc taacttctct ttttcttctt tagggaaatt cttagcatca ggacttgcta atatctcttt gatggtatta acaatacctt ccccttcatt acgtaattca atacgttcac ggatgatgtt atcccgttcc ttgttagctt cagcatcacg gatcatcttt tggatctcct cctcagaaag attaccgttg tcactaatgg taatactgtt ttccttttgc gtggttaaat ccttagcttt aacatttaag atcccattgg catccaaact aaaggtaatc tcaatttggg gtttaccttt aggtgctggt tgaataccac ctaagttaaa tcttcctaat gacttattat ctctagacat tggtctttcc ccttgacata caaccacatc aactgattct tggttatctt gagcagttga aaagatttga cttttactta ctgggatagt tgtatttctc ttaattaaag gagtagccac accacctaaa gtttcaatag aaagggttaa tggagttaca tctaaaagta aaacatcctt aacatcacca cgtaaaaccc caccttgaat agcagcgcca atagcaacaa cttcatcagg attaatagaa cggtttggtt ttttacctnn nnccattgat tcaactagct tttgaactgc anncatcctt ntnnnnncac caactnnnnn natttcatta atctcttcag gtttaatctt ngcttccttg nnnnnntctg aaatagggtt nnnnnnnnnn nnnnntagtg gttttgttaa ctcctcaaac ttnnnnnngn nnnnnnnnnn nnnnncgtta acannnnnnn nnnnggnnnn ngtnnnnaat ggtagagaan nnnncntntc aagttgagcn nnnnnnncnn nnnnngcacn nnnagctgct tctttaagcc gttgcattgc canctnnncn tttgataagt ttaaaccctg gtgttctttg gcaatgtagg ctgagatata ttcaatgatc ttgttatccc aatcatcacc tcccaaacgg ttgtccccag cagttgcaag tacttcaaaa gtaccttctg caatgtcaag taaagataca tcaaaagttc caccacccaa gtcataaacc aagactttca tctctcttga tgctttatca atcccataag ctaaagcagc agcagttggt tcgttaatga tcctttcaac atttaaacca gcaatcttac ctgcggtttt agtagcgttt ctttccgcat cattaaagta tgcaggaaca gtaataactg ctcttgaaat ctttttacca atcttttttt cagcaaagtc cttaagataa ctaaggattt gcgctgaaac ttgttcagga cttaattcct tagttgtacc atcagcattt tggactttta ctttatttga ggtacccatc aacctcttga tggagacaat ggtatttggg tttgtaacca tctgtctttt agcagcatca cctactataa tttcattgtt tttataggaa acaatggaag gtgttgttct tttaccttca ggattttcta atacaacagg tctcccacct tccattacag aaacacaaga attggtagtt ccaaggtcaa tgccaataat taaaccattg tctgcactca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMS ADNGLIIGID LGTTNSCVSV MEGGRPVVLE NPEGKRTTPS IVSYKNNEII VGDAAKRQMV TNPNTIVSIK RLMGTSNKVK VQNADGTTKE LSPEQVSAQI LSYLKDFAEK KIGKKISRAV ITVPAYFNDA ERNATKTAGK IAGLNVERII NEPTAAALAY GIDKASREMK VLVYDLGGGT FDVSLLDIAE GTFEVLATAG DNRLGGDDWD NKIIEYISAY IAKEHQGLNL SKDKMAMQRL KEAAERAKIE LSAQLETIIS LPFLTVTQKG PVNVELKLTR AKFEELTKPL LERTRNPISD VIKEAKIKPE EINEILLVGG STRMPAVQKL VESMVPGKKP NRSINPDEVV AIGAAIQGGV LRGDVKDVLL LDVTPLTLSI ETLGGVATPL IKRNTTIPVS KSQIFSTAQD NQESVDVVVC QGERPMSRDN KSLGRFNLGG IQPAPKGKPQ IEITFSLDAN GILNVKAKDL TTQKENSITI SDNGNLSEEE IQKMIRDAEA NKERDNIIRE RIELRNEGEG IVNTIKEILA SPDAKNFPKE EKEKLEKLTG NIDAAIKAND YAKLKVEIEN FKKWREEMAK KYNPTGEQGP QAK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTG CAGACAATGG TTTAATTATT GGCATTGACC TTGGAACTAC CAATTCTTGT GTTTCTGTAA TGGAAGGTGG GAGACCTGTT GTATTAGAAA ATCCTGAAGG TAAAAGAACA ACACCTTCCA TTGTTTCCTA TAAAAACAAT GAAATTATAG TAGGTGATGC TGCTAAAAGA CAGATGGTTA CAAACCCAAA TACCATTGTC TCCATCAAGA GGTTGATGGG TACCTCAAAT AAAGTAAAAG TCCAAAATGC TGATGGTACA ACTAAGGAAT TAAGTCCTGA ACAAGTTTCA GCGCAAATCC TTAGTTATCT TAAGGACTTT GCTGAAAAAA AGATTGGTAA AAAGATTTCA AGAGCAGTTA TTACTGTTCC TGCATACTTT AATGATGCGG AAAGAAACGC TACTAAAACC GCAGGTAAGA TTGCTGGTTT AAATGTTGAA AGGATCATTA ACGAACCAAC TGCTGCTGCT TTAGCTTATG GGATTGATAA AGCATCAAGA GAGATGAAAG TCTTGGTTTA TGACTTGGGT GGTGGAACTT TTGATGTATC TTTACTTGAC ATTGCAGAAG GTACTTTTGA AGTACTTGCA ACTGCTGGGG ACAACCGTTT GGGAGGTGAT GATTGGGATA ACAAGATCAT TGAATATATC TCAGCCTACA TTGCCAAAGA ACACCAGGGT TTAAACTTAT CAAAAGATAA GATGGCAATG CAACGGCTTA AAGAAGCAGC TGAACGTGCT AAGATTGAAC TTTCCGCTCA ACTTGAAACG ATTATTTCTC TACCATTTTT AACTGTTACC CAAAAAGGTC CTGTTAACGT TGAGTTAAAA CTAACCCGTG CTAAGTTTGA GGAGTTAACA AAACCACTAC TTGAAAGAAC AAGAAACCCT ATTTCAGATG TTATCAAGGA AGCTAAGATT AAACCTGAAG AGATTAATGA AATTCTTTTA GTTGGTGGTT CTACAAGGAT GCCTGCAGTT CAAAAGCTAG TTGAATCAAT GGTACCAGGT AAAAAACCAA ACCGTTCTAT TAATCCTGAT GAAGTTGTTG CTATTGGCGC TGCTATTCAA GGTGGGGTTT TACGTGGTGA TGTTAAGGAT GTTTTACTTT TAGATGTAAC TCCATTAACC CTTTCTATTG AAACTTTAGG TGGTGTGGCT ACTCCTTTAA TTAAGAGAAA TACAACTATC CCAGTAAGTA AAAGTCAAAT CTTTTCAACT GCTCAAGATA ACCAAGAATC AGTTGATGTG GTTGTATGTC AAGGGGAAAG ACCAATGTCT AGAGATAATA AGTCATTAGG AAGATTTAAC TTAGGTGGTA TTCAACCAGC ACCTAAAGGT AAACCCCAAA TTGAGATTAC CTTTAGTTTG GATGCCAATG GGATCTTAAA TGTTAAAGCT AAGGATTTAA CCACGCAAAA GGAAAACAGT ATTACCATTA GTGACAACGG TAATCTTTCT GAGGAGGAGA TCCAAAAGAT GATCCGTGAT GCTGAAGCTA ACAAGGAACG GGATAACATC ATCCGTGAAC GTATTGAATT ACGTAATGAA GGGGAAGGTA TTGTTAATAC CATCAAAGAG ATATTAGCAA GTCCTGATGC TAAGAATTTC CCTAAAGAAG AAAAAGAGAA GTTAGAAAAG CTAACAGGTA ACATTGATGC TGCTATTAAA GCTAATGACT ATGCCAAACT CAAAGTGGAA ATTGAAAACT TTAAGAAGTG AAGAGAAGAG ATGGCAAAAA AATATAACCC AACTGGTGAA CAAGGTCCAC AAGCAAAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT