MygeA.20189.a

Lon protease (MG_239)

CENTER ID: MygeA.20189.a
ORGANISM: Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MygeA.20189.a.A11.GE43497 Full length(MygeA.20189.a) 1 795
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_81219
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|243273.25.peg.276
UniProt: P47481
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVTKKSQIL VVRGQVIFPF VPFSLDVGRP RSRKIIKALK TLKTKRLVLV TQKFTGEQNP EFNDIYHVGT LCEIDEIVDV PGVDSKTVDY RIKGRGLQRV LIEKFSDADI NEVSYQLLNS TVKDEANVDR FLQRIFPEKE EIEQLMEGAE KFLELENISK TVNVPKGLKQ LDIITFKLAN LVPNTESIKQ AILEENEIAN RLEKIIQAGI EDLQKIQDYG RSKNKETEFD KLDSKITRKI NEQLSRQQRD FYLREKLRII REEIGISSKK EDEVASIRKK LDENPYPEAI KKRILSELEH YENSSSSSQE STLTKTYIDT LLNLPWWQKS KDNSDVKNLI KTLDKNHTGL DKVKERIVEY LAVQLRTQKN KGPIMCLVGP PGVGKSSLAK SIAEALDKKF VKISLGGVHD ESEIRGHRKT YLGSMPGRIL KGMTRAKVIN PLFLLDEIDK MTSSNQGYPS GALLEVLDPE LNNKFSDNYV EEDYDLSKVM FIATANYIED IPEALLDRME IIELTSYTEQ EKIEIAKNHL IKRCLEDADL NSEELKFTDE AISYIIKFYT REAGVRQLER LIQQVVRKYI VAMQKDGIKQ ETIDVNAVKK YLKKEIFDHT MRDEVSLPGI VNGMAYTPTG GDLLPIEVTH VAGKGELILT GNLKQTMRES ANVALGYVKA NAERFNINPS LFKKIDINIH VPGGGIPKDG PSAGAALVTA IISSLTGKKV DPTVAMTGEI TLRGKVLVIG GVKEKTISAY RGGVTTIFMP EKNERYLDEV PKEIVDKLNI IFVKEYSDIY NKLFS
NT Sequence
ATGCCCGTTA CGAAGAAAAG TCAGATCTTA GTAGTTAGAG GTCAAGTCAT TTTTCCTTTT GTTCCCTTTA GTTTGGATGT TGGCAGGCCC CGTTCGCGTA AGATCATCAA AGCGCTTAAA ACTCTGAAAA CCAAACGTTT GGTTTTAGTA ACCCAAAAGT TTACTGGTGA ACAAAACCCT GAGTTTAATG ACATCTATCA TGTCGGTACA CTCTGTGAGA TTGATGAGAT AGTTGATGTT CCAGGGGTTG ATAGTAAAAC AGTAGACTAC CGTATTAAAG GCAGAGGTTT ACAACGGGTT TTAATTGAAA AATTCTCAGA TGCAGATATT AATGAAGTTA GTTACCAATT ACTTAACTCC ACAGTTAAAG ATGAAGCTAA TGTTGACAGG TTCTTACAGC GAATCTTTCC TGAAAAAGAA GAAATTGAAC AGTTAATGGA AGGAGCTGAG AAGTTTTTAG AACTTGAAAA CATCAGCAAA ACAGTTAATG TTCCTAAGGG TTTAAAGCAA CTTGATATTA TCACCTTTAA ACTGGCTAAT CTTGTCCCTA ACACTGAAAG TATTAAACAA GCTATCTTAG AGGAAAATGA GATAGCAAAC CGATTGGAAA AGATTATCCA AGCAGGGATT GAAGATTTAC AGAAGATCCA AGATTATGGT AGATCTAAAA ACAAGGAAAC TGAGTTTGAT AAACTTGACA GTAAAATTAC CCGCAAAATT AACGAACAAC TCTCAAGACA ACAACGTGAT TTCTATCTTC GTGAAAAGCT AAGAATTATC CGTGAAGAGA TAGGGATTAG TTCCAAAAAA GAGGATGAAG TTGCTAGTAT TAGAAAGAAA CTGGATGAAA ACCCTTACCC TGAAGCCATT AAAAAACGGA TTTTAAGTGA ACTTGAACAC TATGAAAACT CTTCCTCCTC TTCCCAAGAA TCAACCTTAA CCAAAACTTA CATTGATACG CTTTTAAACC TGCCTTGATG ACAAAAGAGC AAAGATAACA GTGATGTTAA AAACTTAATT AAGACGTTAG ATAAAAACCA CACTGGTTTA GATAAGGTTA AAGAAAGGAT TGTTGAGTAT TTAGCAGTAC AACTAAGAAC CCAAAAAAAC AAAGGTCCTA TTATGTGTTT AGTAGGTCCT CCTGGGGTTG GTAAATCAAG TCTAGCTAAG TCTATTGCAG AAGCATTAGA TAAGAAGTTT GTCAAGATCT CATTAGGGGG AGTACATGAT GAATCGGAAA TCAGAGGTCA CCGTAAAACT TACTTAGGTT CTATGCCAGG AAGGATTTTG AAAGGGATGA CCCGTGCTAA GGTAATTAAT CCCCTCTTTT TACTTGATGA AATTGATAAG ATGACCTCCT CCAACCAAGG TTATCCTTCA GGTGCTTTAC TTGAAGTATT AGATCCAGAG TTAAATAATA AGTTTAGTGA TAACTATGTT GAAGAAGATT ATGATCTTTC TAAAGTAATG TTTATCGCTA CTGCAAACTA CATAGAAGAT ATCCCTGAAG CTTTACTTGA TAGGATGGAG ATAATTGAAC TCACTTCCTA TACAGAACAA GAGAAGATTG AGATAGCAAA AAACCACTTA ATTAAGCGTT GCCTTGAGGA TGCTGATCTT AACAGTGAAG AATTGAAGTT CACTGATGAA GCAATCAGCT ACATCATTAA GTTTTACACA AGAGAAGCGG GGGTTAGACA ATTAGAACGA TTAATCCAAC AAGTTGTAAG AAAGTACATA GTAGCAATGC AAAAAGATGG CATCAAACAA GAAACGATTG ATGTAAACGC TGTTAAAAAA TACCTTAAGA AGGAGATCTT TGATCACACT ATGCGTGATG AAGTGTCTCT ACCTGGAATT GTCAACGGGA TGGCATACAC CCCAACTGGA GGGGACTTAC TTCCCATAGA AGTTACCCAT GTTGCTGGTA AAGGAGAGTT GATCTTAACT GGTAATTTAA AGCAAACAAT GCGAGAAAGC GCTAATGTTG CTTTAGGCTA TGTAAAAGCT AATGCAGAGC GTTTTAACAT TAATCCTAGT TTGTTTAAAA AGATTGATAT TAACATCCAT GTTCCAGGTG GGGGAATTCC TAAGGATGGA CCTAGTGCTG GTGCTGCTTT GGTAACTGCA ATCATCTCAT CATTAACTGG TAAGAAAGTA GATCCTACAG TGGCTATGAC AGGAGAGATC ACTTTAAGAG GCAAAGTGTT GGTTATTGGT GGGGTGAAAG AAAAAACTAT CTCAGCTTAC CGCGGTGGGG TTACAACTAT CTTTATGCCT GAGAAAAACG AGCGCTATTT AGATGAAGTA CCCAAAGAGA TAGTAGATAA ACTTAACATT ATCTTTGTTA AGGAATACAG TGATATCTAC AACAAGCTTT TCAGT
Details for MygeA.20189.a.A11.GE43497
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 92kDa
OBSERVED MW: 92kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPVTKKSQI LVVRGQVIFP FVPFSLDVGR PRSRKIIKAL KTLKTKRLVL VTQKFTGEQN PEFNDIYHVG TLCEIDEIVD VPGVDSKTVD YRIKGRGLQR VLIEKFSDAD INEVSYQLLN STVKDEANVD RFLQRIFPEK EEIEQLMEGA EKFLELENIS KTVNVPKGLK QLDIITFKLA NLVPNTESIK QAILEENEIA NRLEKIIQAG IEDLQKIQDY GRSKNKETEF DKLDSKITRK INEQLSRQQR DFYLREKLRI IREEIGISSK KEDEVASIRK KLDENPYPEA IKKRILSELE HYENSSSSSQ ESTLTKTYID TLLNLPWWQK SKDNSDVKNL IKTLDKNHTG LDKVKERIVE YLAVQLRTQK NKGPIMCLVG PPGVGKSSLA KSIAEALDKK FVKISLGGVH DESEIRGHRK TYLGSMPGRI LKGMTRAKVI NPLFLLDEID KMTSSNQGYP SGALLEVLDP ELNNKFSDNY VEEDYDLSKV MFIATANYIE DIPEALLDRM EIIELTSYTE QEKIEIAKNH LIKRCLEDAD LNSEELKFTD EAISYIIKFY TREAGVRQLE RLIQQVVRKY IVAMQKDGIK QETIDVNAVK KYLKKEIFDH TMRDEVSLPG IVNGMAYTPT GGDLLPIEVT HVAGKGELIL TGNLKQTMRE SANVALGYVK ANAERFNINP SLFKKIDINI HVPGGGIPKD GPSAGAALVT AIISSLTGKK VDPTVAMTGE ITLRGKVLVI GGVKEKTISA YRGGVTTIFM PEKNERYLDE VPKEIVDKLN IIFVKEYSDI YNKLFS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccggtgacc aaaaagagcc aaatcctggt cgtccgcggt caggtcattt tccctttcgt cccattcagc ctggacgtcg gtcgtccgcg tagccgtaaa atcatcaaag ctcttaagac tctgaaaacg aaacgtctgg tattggttac gcagaaattc accggtgagc agaatccgga gttcaacgac atttatcacg ttggcaccct gtgtgaaatt gacgaaattg tcgacgttcc tggcgtcgac agcaagaccg tggactaccg catcaagggc cgcggcctcc agcgcgtcct gattgagaag ttcagcgacg cggacattaa tgaagtctcc tatcagctgc tgaacagcac ggttaaggac gaagcaaacg tggaccgttt cctgcagcgc atctttccgg aaaaggaaga gattgagcag ttgatggagg gtgcggagaa attcctggaa ttggaaaata ttagcaagac cgtgaacgtc ccgaagggtc tgaaacagct ggacatcatc accttcaaac tggcgaatct ggtcccgaat accgaaagca ttaagcaagc gatcctggag gaaaacgaga tcgcgaatcg tttggagaaa atcatccaag cgggtatcga ggatctgcaa aagatccagg attacggtcg ctccaagaac aaagaaaccg aatttgataa gctggatagc aaaatcaccc gtaagattaa cgaacagctg agccgccagc agcgcgattt ctatctgcgt gaaaaactgc gtatcatccg tgaagaaatc ggcatctcca gcaagaagga ggatgaggtt gcgagcattc gtaagaaact ggatgagaac ccgtacccgg aagcgatcaa gaaacgcatt ttgtctgaac tggaacatta tgagaatagc agctcctcga gccaagagag caccctgacc aaaacgtata ttgatacgct gctgaatctg ccgtggtggc agaaatccaa ggacaactcc gatgtcaaga atctgatcaa aaccctggat aagaaccaca ccggtctgga caaagttaaa gagcgtatcg ttgagtatct ggctgttcag ttgcgcaccc agaaaaacaa gggtccgatt atgtgtctcg ttggtccgcc gggtgtgggc aaatcttccc tggccaaatc tatcgcagaa gccctggaca agaagtttgt caagatctct ctgggtggcg ttcacgacga gagcgagatc cgtggtcacc gcaaaaccta cctgggcagc atgccgggtc gtattctgaa aggtatgacc cgcgcgaagg tgatcaaccc gctgttcctg ctggacgaga tcgacaagat gacctccagc aaccaaggct acccgagcgg cgccctcctg gaggtgctgg acccggaact gaacaataag ttctccgaca attatgttga ggaggactat gacttgagca aagttatgtt tatcgctacg gcaaattata tcgaggatat tccggaggcg ctgctggacc gtatggaaat cattgaactg actagctaca cggaacagga gaagattgag attgcgaaaa atcatctgat caagcgttgc ctggaagatg ctgacttgaa ctccgaggaa ctgaaattca ccgatgaggc gatctcctat atcattaagt tttacacccg tgaagcgggt gttcgtcagc tggagcgcct gattcaacag gtggtgcgta aatatattgt cgcaatgcag aaagatggca ttaaacagga aaccatcgac gttaatgccg tgaaaaaata cctgaagaag gaaatcttcg atcatacgat gcgtgatgag gtgagcctgc caggtattgt taacggtatg gcctataccc cgacgggcgg tgacctgttg ccgattgagg tgacccatgt cgccggcaaa ggtgagctga ttctgacggg taatctgaaa cagactatgc gcgagagcgc caatgttgcg ctgggttatg tgaaagcaaa tgcggaacgt ttcaacatta acccgagcct gtttaagaaa attgacatca atatccatgt gccgggcggt ggcatcccta aggacggtcc gtccgcgggt gcagcgctgg tcaccgcgat tattagctct ctgactggta agaaagtgga cccgacggtg gcaatgaccg gtgagattac cctgcgtggc aaagtactgg tcattggtgg tgtcaaggaa aagaccattt ccgcgtaccg cggcggtgtc accacgattt tcatgccaga aaagaacgaa agatacctgg atgaggttcc gaaagagatt gtcgacaagc tcaatattat ctttgtgaaa gaatatagcg acatttacaa caagctgttt tcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa