MygeA.20174.a

Uncharacterized protein MG269 (MPN387) (MG_269)

CENTER ID: MygeA.20174.a
ORGANISM: Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 C

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MygeA.20174.a.B2.GE42355 N-term domain 18 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|243273.25.peg.310
UniProt: Q49407
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNFDYYRDF DDFQRRETIN FFLAKFPFAS KAQLDVFLEQ AKTAYVKLRT SNPKNVDWNQ MLVLLLKKFG PEARSEKERL KRILSLQEQL KVRLEGEINR QVQQNSELFS QLKQSESEII QMQQLVEAKE HQIEALNKQL HAIKEANKKL IEEHESINVE ELLKEYEVQC NEAIYKRDQH IQTVFEDKLA LKDGEISETQ SLLKTAEKEK QALKKAYKLV VNSLQKHQKL TTEIEIDFTK LDEIIATIFD ETKNPKTGFT NFIKQFEKTK AKLTKKIAEI TKLDHSTPTN YQQETPASQQ QLDQENEPIK PSKKSNSSSL PRGTTQPKSN SINRVSKLID
NT Sequence
ATGAATAATT TTGATTACTA TAGAGACTTT GATGACTTTC AAAGAAGAGA AACTATTAAC TTCTTTTTAG CCAAGTTTCC CTTTGCTTCC AAAGCCCAGC TTGATGTTTT TTTAGAACAA GCAAAAACTG CTTATGTCAA ACTACGAACA AGCAACCCAA AAAATGTTGA TTGGAACCAG ATGCTTGTTT TGTTATTAAA AAAGTTTGGT CCTGAAGCGA GAAGTGAAAA AGAACGTTTA AAAAGGATCT TAAGTTTGCA AGAACAACTT AAGGTACGCT TGGAAGGAGA GATTAACCGT CAAGTTCAAC AGAACAGCGA ACTTTTCTCA CAACTCAAAC AAAGTGAAAG TGAAATTATC CAAATGCAGC AGCTGGTAGA AGCAAAAGAA CACCAAATTG AAGCATTAAA TAAACAGTTA CATGCAATAA AAGAAGCCAA TAAGAAACTA ATTGAAGAAC ATGAAAGTAT TAATGTAGAG GAATTGTTAA AGGAATATGA AGTGCAGTGT AATGAAGCAA TTTATAAACG TGATCAACAC ATCCAAACCG TATTTGAAGA CAAGTTAGCA CTTAAAGATG GGGAAATTAG TGAAACCCAA TCACTTTTAA AAACCGCTGA AAAAGAAAAG CAAGCATTAA AAAAAGCATA TAAGTTAGTT GTAAATTCGC TTCAAAAACA CCAAAAGCTC ACCACCGAAA TAGAAATTGA TTTCACCAAG CTTGATGAGA TTATTGCAAC CATTTTTGAT GAAACTAAGA ATCCAAAGAC TGGCTTTACT AACTTCATTA AGCAGTTTGA AAAAACCAAA GCAAAACTAA CAAAAAAGAT AGCTGAAATT ACTAAACTTG ATCATTCAAC GCCAACAAAT TATCAACAAG AAACTCCAGC GTCACAACAG CAATTAGATC AAGAGAATGA ACCGATCAAA CCATCTAAAA AATCAAATTC AAGTTCATTG CCTAGAGGCA CTACACAACC CAAATCCAAT TCTATTAACA GGGTTAGTAA ATTAATTGAT
Details for MygeA.20174.a.B2.GE42355
HARVESTED ON: data unavailable
SEQUENCED ON: 4/18/2018
EXPECTED MW: 32kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT INFFLAKFPF ASKAQLDVFL EQAKTAYVKL RTSNPKNVDW NQMLVLLLKK FGPEARSEKE RLKRILSLQE QLKVRLEGEI NRQVQQNSEL FSQLKQSESE IIQMQQLVEA KEHQIEALNK QLHAIKEANK KLIEEHESIN VEELLKEYEV QCNEAIYKRD QHIQTVFEDK LALKDGEISE TQSLLKTAEK EKQALKKAYK LVVNSLQKHQ KLTTEIEIDF TKLDEIIATI FDETKNPKTG FTNFIKQFEK TKAKLTXXXA XITKLD
Validated NT Sequence
atcttactca cttaatcaag tttagtaatt tnngcnnnnn nntttgttag ttttgctttg gttttttcaa actgcttaat gaagttagta aagccagtct ttggattctt agtttcatca aaaatggttg caataatctc atcaagcttg gtgaaatcaa tttctatttc ggtggtgagc ttttggtgtt tttgaagcga atttacaact aacttatatg ctttttttaa tgcttgcttt tctttttcag cggtttttaa aagtgattgg gtttcactaa tttccccatc tttaagtgct aacttgtctt caaatacggt ttggatgtgt tgatcacgtt tataaattgc ttcattacac tgcacttcat attcctttaa caattcctct acattaatac tttcatgttc ttcaattagt ttcttattgg cttcttttat tgcatgtaac tgtttattta atgcttcaat ttggtgttct tttgcttcta ccagctgctg catttggata atttcacttt cactttgttt gagttgtgag aaaagttcgc tgttctgttg aacttgacgg ttaatctctc cttccaagcg taccttaagt tgttcttgca aacttaagat cctttttaaa cgttcttttt cacttctcgc ttcaggacca aactttttta ataacaaaac aagcatctgg ttccaatcaa cattttttgg gttgcttgtt cgtagtttga cataagcagt ttttgcttgt tctaaaaaaa catcaagctg ggctttggaa gcaaagggaa acttggctaa aaagaagtta atagtcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHTI NFFLAKFPFA SKAQLDVFLE QAKTAYVKLR TSNPKNVDWN QMLVLLLKKF GPEARSEKER LKRILSLQEQ LKVRLEGEIN RQVQQNSELF SQLKQSESEI IQMQQLVEAK EHQIEALNKQ LHAIKEANKK LIEEHESINV EELLKEYEVQ CNEAIYKRDQ HIQTVFEDKL ALKDGEISET QSLLKTAEKE KQALKKAYKL VVNSLQKHQK LTTEIEIDFT KLDEIIATIF DETKNPKTGF TNFIKQFEKT KAKLTKKIAE ITKLD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catactatta acttcttttt agccaagttt ccctttgctt ccaaagccca gcttgatgtt tttttagaac aagcaaaaac tgcttatgtc aaactacgaa caagcaaccc aaaaaatgtt gattggaacc agatgcttgt tttgttatta aaaaagtttg gtcctgaagc gagaagtgaa aaagaacgtt taaaaaggat cttaagtttg caagaacaac ttaaggtacg cttggaagga gagattaacc gtcaagttca acagaacagc gaacttttct cacaactcaa acaaagtgaa agtgaaatta tccaaatgca gcagctggta gaagcaaaag aacaccaaat tgaagcatta aataaacagt tacatgcaat aaaagaagcc aataagaaac taattgaaga acatgaaagt attaatgtag aggaattgtt aaaggaatat gaagtgcagt gtaatgaagc aatttataaa cgtgatcaac acatccaaac cgtatttgaa gacaagttag cacttaaaga tggggaaatt agtgaaaccc aatcactttt aaaaaccgct gaaaaagaaa agcaagcatt aaaaaaagca tataagttag ttgtaaattc gcttcaaaaa caccaaaagc tcaccaccga aatagaaatt gatttcacca agcttgatga gattattgca accatttttg atgaaactaa gaatccaaag actggcttta ctaacttcat taagcagttt gaaaaaacca aagcaaaact aacaaaaaag atagctgaaa ttactaaact tgattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat