SSGCID: Target Page

MygeA.20091.a

2,3-bisphosphoglycerate-independent phosphoglycerate mutase (MG_430)

CENTER ID:	MygeA.20091.a
ORGANISM:	Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MygeA.20091.a.B1.GE42354	Full length(MygeA.20091.a)		1	507

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MygeA.20091.a.B1.PW38481	Full length(MygeA.20091.a)		1	507

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_81212

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243273.25.peg.496
UniProt:	P47669

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHKKVLLAIL DGYGISNAIY GNAVQNANTP MLDELINSYP CVLLDASGEA VGLPMGQIGN SEVGHLNIGA GRVVYTGLSL INQHIKDRSF FANKAFLKTI EHVEKNHSKI HLIGLFSNGG VHSHNEHLLA LIELFSKHAK VVLHLFGDGR DVAPCSLKQD LEKLMIFLKN YPNVVIGTIG GRYYGMDRDQ RWDREMIAYK ALLGVSKNKF NDPIGYIETQ YQNQITDEFI YPAINANLNS DQFALNNNDG VISFNFRPDR ARQMSHLIFN SNYYNYQPEL KRKENLFFVT MMNYEGIVPS EFAFPPQTIK NSLGEVIANN NLKQLRIAET EKYAHVTFFF DGGFEVNLSN ETKTLIPSLK VATYDLAPEM SCKAITDALL EKLNNFDFTV LNFANPDMVG HTGNYQACIK ALEALDVQIK RIVDFCKANQ ITMFLTADHG NAEVMIDNNN NPVTKHTINP VPFVCTDKNV NFNQTGILAN IAPTILEYLN LSKPKEMTAK SLLKNNN

NT Sequence

ATGCATAAAA AAGTTTTATT AGCAATCCTT GATGGTTATG GGATCTCAAA TGCTATTTAT GGTAATGCAG TACAAAATGC AAATACCCCA ATGCTAGATG AATTAATCAA TTCATATCCT TGTGTACTTT TAGATGCATC TGGGGAAGCA GTTGGATTGC CTATGGGTCA AATAGGTAAC TCTGAGGTAG GTCATCTAAA TATTGGGGCA GGTCGAGTTG TTTATACTGG ACTTTCTTTG ATTAATCAAC ATATTAAGGA TCGTAGTTTT TTTGCAAATA AAGCTTTTTT AAAAACCATA GAACATGTAG AAAAAAACCA TTCAAAAATC CATTTAATTG GGTTATTTTC CAATGGAGGA GTGCATAGTC ATAATGAACA TCTATTAGCA CTCATTGAAT TGTTTTCAAA ACATGCAAAG GTAGTATTAC ATTTATTTGG TGATGGTAGA GATGTAGCAC CTTGTAGCTT AAAACAAGAT CTTGAGAAAT TAATGATATT TCTAAAAAAC TATCCTAATG TTGTTATTGG AACTATTGGG GGAAGATACT ATGGAATGGA TCGTGATCAA CGCTGGGATC GTGAAATGAT TGCTTATAAA GCTTTATTAG GAGTTTCAAA AAATAAATTC AATGACCCAA TTGGTTATAT TGAAACGCAA TATCAGAACC AAATTACTGA TGAATTTATT TATCCTGCAA TTAATGCCAA TTTAAATTCT GATCAGTTTG CATTAAACAA TAATGATGGA GTTATTTTCT TTAATTTTAG ACCTGATAGA GCAAGACAAA TGTCCCATTT GATCTTTAAC AGCAATTATT ACAACTATCA ACCTGAATTG AAACGAAAAG AAAATTTATT TTTTGTAACA ATGATGAATT ATGAGGGAAT TGTACCTAGC GAATTTGCTT TTCCACCTCA AACCATTAAA AATAGTCTTG GTGAAGTAAT TGCTAATAAT AATTTGAAGC AATTGAGGAT TGCAGAAACT GAAAAGTATG CTCACGTTAC TTTCTTTTTT GATGGTGGTT TTGAAGTTAA TCTCAGCAAT GAAACAAAGA CATTAATTCC TTCTTTAAAA GTTGCTACAT ATGATTTAGC TCCCGAAATG TCATGTAAAG CTATTACTGA TGCACTACTA GAAAAGCTTA ATAACTTTGA TTTTACTGTT TTAAATTTTG CTAATCCTGA TATGGTAGGT CATACTGGTA ACTATCAAGC TTGCATTAAA GCTCTTGAAG CACTCGATGT TCAAATTAAA CGAATAGTTG ATTTTTGTAA AGCTAATCAA ATAACTATGT TTTTAACTGC AGATCATGGG AATGCAGAAG TGATGATTGA TAATAATAAC AATCCAGTTA CTAAACACAC TATTAATCCT GTACCATTTG TATGTACTGA CAAAAATGTT AACTTTAATC AAACTGGAAT TTTAGCTAAT ATTGCTCCTA CTATCTTGGA ATATCTTAAC CTTAGCAAAC CAAAAGAGAT GACTGCAAAA TCCTTATTAA AAAATAACAA T

Details for MygeA.20091.a.B1.GE42354

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMH KKVLLAILDG YGISNAIYGN AVQNANTPML DELINSYPCV LLDASGEAVG LPMGQIGNSE VGHLNIGAGR VVYTGLSLIN QHIKDRSFFA NKAFLKTIEH VEKNHSKIHL IGLFSNGGVH SHNEHLLALI ELFSKHAKVV LHLFGDGRDV APCSLKQDLE KLMIFLKNYP NVVIGTIGGR YYGMDRDQRW DREMIAYKAL LGVSKNKFND PIGYIETQYQ NQITDEFIYP AINANLNSDQ FALNNNDGVI FFNFRPDRAR QMSHLIFNSN YYNYQPELKR KENLFFVTMM NYEGIVPSEF AFPPQTIKNS LGEVIANNNL KQLRIAETEK YAHVTFFFDG GFEVNLSNET KTLIPSLKVA TYDLAPEMSC KAITDALLEK LNNFDFTVLN FANPDMVGHT GNYQACIKAL EALDVQIKRI VDFCKANQIT MFLTADHGNA EVMIDNNNNP VTKHTINPVP FVCTDKNVNF NQTGILANIA PTILEYLNLS KPKEMTA

Validated NT Sequence

tttgcagtca tctcttttgg tttgctaagg ttaagatatt ccaagatagt aggagcaata ttagctaaaa ttccagtttg attaaagtta acatttttgt cagtacatac aaatggtaca ggattaatag tgtgtttagt aactggattg ttattattat caatcatcac ttctgcattc ccatgatctg cagttaaaaa catagttatt tgattagctt tacaaaaatc aactattcgt ttaatttgaa catcgagtgc ttcaagagct ttaatgcaag cttgatagtt accagtatga cctaccatat caggattagc aaaatttaaa acagtaaaat caaagttatt aagcttttct agtagtgcat cagtaatagc tttacatgac atttcgggag ctaaatcata tgtagcaact tttaaagaag gaattaatgt ctttgtttca ttgctgagat taacttcaaa accaccatca aaaaagaaag taacgtgagc atacttttca gtttctgcaa tcctcaattg cttcaaatta ttattagcaa ttacttcacc aagactattt ttaatggttt gaggtggaaa agcaaattcg ctaggtacaa ttccctcata attcatcatt gttacaaaaa ataaattttc ttttcgtttc aattcaggtt gatagttgta ataattgctg ttaaagatca aatgggacat ttgtcttgct ctatcaggtc taaaattaaa gaaaataact ccatcattat tgtttaatgc aaactgatca gaatttaaat tggcattaat tgcaggataa ataaattcat cagtaatttg gttctgatat tgcgtttcaa tataaccaat tgggtcattg aatttatttt ttgaaactcc taataaagct ttataagcaa tcatttcacg atcccagcgt tgatcacgat ccattccata gtatcttccc ccaatagttc caataacaac attaggatag ttttttagaa atatcattaa tttctcaaga tcttgtttta agctacaagg tgctacatct ctaccatcac caaataaatg taatactacc tttgcatgtt ttgaaaacaa ttcaatgagt gctaatagat gttcattatg actatgcact cctccattgg aaaataaccc aattaaatgg atttttgaat ggtttttttc tacatgttct atggttttta aaaaagcttt atttgcaaaa aaactacgat ccttaatatg ttgattaatc aaagaaagtc cagtataaac aactcgacct gccccaatat ttagatgacc tacctcagag ttacctattt gacccatagg caatccaact gcttccccag atgcatctaa aagtacacaa ggatatgaat tgattaattc atctagcatt ggggtatttg cattttgtac tgcattacca taaatagcat ttgagatccc ataaccatca aggattgcta ataaaacttt tttatgcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMH KKVLLAILDG YGISNAIYGN AVQNANTPML DELINSYPCV LLDASGEAVG LPMGQIGNSE VGHLNIGAGR VVYTGLSLIN QHIKDRSFFA NKAFLKTIEH VEKNHSKIHL IGLFSNGGVH SHNEHLLALI ELFSKHAKVV LHLFGDGRDV APCSLKQDLE KLMIFLKNYP NVVIGTIGGR YYGMDRDQRW DREMIAYKAL LGVSKNKFND PIGYIETQYQ NQITDEFIYP AINANLNSDQ FALNNNDGVI SFNFRPDRAR QMSHLIFNSN YYNYQPELKR KENLFFVTMM NYEGIVPSEF AFPPQTIKNS LGEVIANNNL KQLRIAETEK YAHVTFFFDG GFEVNLSNET KTLIPSLKVA TYDLAPEMSC KAITDALLEK LNNFDFTVLN FANPDMVGHT GNYQACIKAL EALDVQIKRI VDFCKANQIT MFLTADHGNA EVMIDNNNNP VTKHTINPVP FVCTDKNVNF NQTGILANIA PTILEYLNLS KPKEMTAKSL LKNNN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata aaaaagtttt attagcaatc cttgatggtt atgggatctc aaatgctatt tatggtaatg cagtacaaaa tgcaaatacc ccaatgctag atgaattaat caattcatat ccttgtgtac ttttagatgc atctggggaa gcagttggat tgcctatggg tcaaataggt aactctgagg taggtcatct aaatattggg gcaggtcgag ttgtttatac tggactttct ttgattaatc aacatattaa ggatcgtagt ttttttgcaa ataaagcttt tttaaaaacc atagaacatg tagaaaaaaa ccattcaaaa atccatttaa ttgggttatt ttccaatgga ggagtgcata gtcataatga acatctatta gcactcattg aattgttttc aaaacatgca aaggtagtat tacatttatt tggtgatggt agagatgtag caccttgtag cttaaaacaa gatcttgaga aattaatgat atttctaaaa aactatccta atgttgttat tggaactatt gggggaagat actatggaat ggatcgtgat caacgctggg atcgtgaaat gattgcttat aaagctttat taggagtttc aaaaaataaa ttcaatgacc caattggtta tattgaaacg caatatcaga accaaattac tgatgaattt atttatcctg caattaatgc caatttaaat tctgatcagt ttgcattaaa caataatgat ggagttattt tctttaattt tagacctgat agagcaagac aaatgtccca tttgatcttt aacagcaatt attacaacta tcaacctgaa ttgaaacgaa aagaaaattt attttttgta acaatgatga attatgaggg aattgtacct agcgaatttg cttttccacc tcaaaccatt aaaaatagtc ttggtgaagt aattgctaat aataatttga agcaattgag gattgcagaa actgaaaagt atgctcacgt tactttcttt tttgatggtg gttttgaagt taatctcagc aatgaaacaa agacattaat tccttcttta aaagttgcta catatgattt agctcccgaa atgtcatgta aagctattac tgatgcacta ctagaaaagc ttaataactt tgattttact gttttaaatt ttgctaatcc tgatatggta ggtcatactg gtaactatca agcttgcatt aaagctcttg aagcactcga tgttcaaatt aaacgaatag ttgatttttg taaagctaat caaataacta tgtttttaac tgcagatcat gggaatgcag aagtgatgat tgataataat aacaatccag ttactaaaca cactattaat cctgtaccat ttgtatgtac tgacaaaaat gttaacttta atcaaactgg aattttagct aatattgctc ctactatctt ggaatatctt aaccttagca aaccaaaaga gatgactgca aaatccttat taaaaaataa caattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for MygeA.20091.a.B1.PW38481

PURIFICATION DATe:	5/29/2018
CONCENTRATION:	46.5mg/ml
OBSERVED MW:	52kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMH KKVLLAILDG YGISNAIYGN AVQNANTPML DELINSYPCV LLDASGEAVG LPMGQIGNSE VGHLNIGAGR VVYTGLSLIN QHIKDRSFFA NKAFLKTIEH VEKNHSKIHL IGLFSNGGVH SHNEHLLALI ELFSKHAKVV LHLFGDGRDV APCSLKQDLE KLMIFLKNYP NVVIGTIGGR YYGMDRDQRW DREMIAYKAL LGVSKNKFND PIGYIETQYQ NQITDEFIYP AINANLNSDQ FALNNNDGVI SFNFRPDRAR QMSHLIFNSN YYNYQPELKR KENLFFVTMM NYEGIVPSEF AFPPQTIKNS LGEVIANNNL KQLRIAETEK YAHVTFFFDG GFEVNLSNET KTLIPSLKVA TYDLAPEMSC KAITDALLEK LNNFDFTVLN FANPDMVGHT GNYQACIKAL EALDVQIKRI VDFCKANQIT MFLTADHGNA EVMIDNNNNP VTKHTINPVP FVCTDKNVNF NQTGILANIA PTILEYLNLS KPKEMTAKSL LKNNN

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATA AAAAAGTTTT ATTAGCAATC CTTGATGGTT ATGGGATCTC AAATGCTATT TATGGTAATG CAGTACAAAA TGCAAATACC CCAATGCTAG ATGAATTAAT CAATTCATAT CCTTGTGTAC TTTTAGATGC ATCTGGGGAA GCAGTTGGAT TGCCTATGGG TCAAATAGGT AACTCTGAGG TAGGTCATCT AAATATTGGG GCAGGTCGAG TTGTTTATAC TGGACTTTCT TTGATTAATC AACATATTAA GGATCGTAGT TTTTTTGCAA ATAAAGCTTT TTTAAAAACC ATAGAACATG TAGAAAAAAA CCATTCAAAA ATCCATTTAA TTGGGTTATT TTCCAATGGA GGAGTGCATA GTCATAATGA ACATCTATTA GCACTCATTG AATTGTTTTC AAAACATGCA AAGGTAGTAT TACATTTATT TGGTGATGGT AGAGATGTAG CACCTTGTAG CTTAAAACAA GATCTTGAGA AATTAATGAT ATTTCTAAAA AACTATCCTA ATGTTGTTAT TGGAACTATT GGGGGAAGAT ACTATGGAAT GGATCGTGAT CAACGCTGGG ATCGTGAAAT GATTGCTTAT AAAGCTTTAT TAGGAGTTTC AAAAAATAAA TTCAATGACC CAATTGGTTA TATTGAAACG CAATATCAGA ACCAAATTAC TGATGAATTT ATTTATCCTG CAATTAATGC CAATTTAAAT TCTGATCAGT TTGCATTAAA CAATAATGAT GGAGTTATTT TCTTTAATTT TAGACCTGAT AGAGCAAGAC AAATGTCCCA TTTGATCTTT AACAGCAATT ATTACAACTA TCAACCTGAA TTGAAACGAA AAGAAAATTT ATTTTTTGTA ACAATGATGA ATTATGAGGG AATTGTACCT AGCGAATTTG CTTTTCCACC TCAAACCATT AAAAATAGTC TTGGTGAAGT AATTGCTAAT AATAATTTGA AGCAATTGAG GATTGCAGAA ACTGAAAAGT ATGCTCACGT TACTTTCTTT TTTGATGGTG GTTTTGAAGT TAATCTCAGC AATGAAACAA AGACATTAAT TCCTTCTTTA AAAGTTGCTA CATATGATTT AGCTCCCGAA ATGTCATGTA AAGCTATTAC TGATGCACTA CTAGAAAAGC TTAATAACTT TGATTTTACT GTTTTAAATT TTGCTAATCC TGATATGGTA GGTCATACTG GTAACTATCA AGCTTGCATT AAAGCTCTTG AAGCACTCGA TGTTCAAATT AAACGAATAG TTGATTTTTG TAAAGCTAAT CAAATAACTA TGTTTTTAAC TGCAGATCAT GGGAATGCAG AAGTGATGAT TGATAATAAT AACAATCCAG TTACTAAACA CACTATTAAT CCTGTACCAT TTGTATGTAC TGACAAAAAT GTTAACTTTA ATCAAACTGG AATTTTAGCT AATATTGCTC CTACTATCTT GGAATATCTT AACCTTAGCA AACCAAAAGA GATGACTGCA AAATCCTTAT TAAAAAATAA CAATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT