MygeA.20067.a

Cytadherence high molecular weight protein 3 (HMW3) (MG317) (MG_317)

CENTER ID:	MygeA.20067.a
ORGANISM:	Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	C

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MygeA.20067.a.B2.GE42352	N-term ordered domain		1	116

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MygeA.20067.a.B2.PW38535	N-term ordered domain		1	116

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_81208

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|243273.25.peg.368
UniProt:	Q57081

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNDKQKAKIN KAYNKLLKKI NKRYPDVSVV YARDHKNKVH ALYQDPESGN IFSLEKRKQL ASNYPLFELT SDNPISFTNN IVSLNAYDDK NNLVTVQYDQ DNNTFYDQNG NVLDVSSYTD EKKVPLINYL SSTQTSQEQP TQQDYPSIDA GLPKIEVDDQ PKAAQHTTLE TESEPDVFEL NDSLNQPQQP TENLGDDQFV EKEVPPTQQL HQDLVHQQPV QVDSGSQNHS FNNSPSLKPP LVNKPAKLVQ PEVKHIPQVE VQPKPQIVEP KIEPKPEVKH VSHVEIQPKP EVKPVVDSVP EVKQPEVKHV PHVEVQPKPV VDLKPQRIEP RIESKPEVIK HIPQVEVQPK AQMVEPRIEP KPETKYIPQV ESTPQVEVHH WKPEVKTEYQ PQQPLPTSGL QIKVVPRSAA LLQSKLDTGF QPRQVERTTD SDITVSVSSH ASLLEKINAL NHQRIMSDIA LKSDNTIKSS NFSRFYPENE YVATKYSDPL YSDTNQSLTS DRFSLDFDYT PKSRVNNYTP LRSTNFQNNA ISNYRFSRTP SSYYPLTRRP WRLTNISSYR SSFHSPTRLS SFRRTSLPFS SSYDGLRRYP SRSYWSKDF

NT Sequence

ATGAACGATA AACAGAAAGC TAAAATAAAC AAAGCCTATA ACAAGCTTTT AAAAAAGATT AACAAAAGAT ATCCTGATGT TAGTGTTGTT TATGCCCGTG ATCATAAAAA TAAGGTTCAT GCTCTATACC AAGATCCTGA ATCAGGTAAT ATCTTCTCTT TAGAAAAAAG AAAGCAGTTA GCTAGTAACT ATCCTTTGTT TGAATTAACT TCAGATAACC CTATTAGCTT TACAAATAAC ATTGTTAGCT TAAATGCTTA TGATGATAAG AACAACTTGG TAACTGTTCA ATATGATCAG GATAACAACA CTTTCTATGA CCAAAATGGT AATGTTTTGG ATGTTTCTAG CTATACTGAT GAGAAAAAGG TCCCTTTAAT TAACTATTTA AGCAGTACTC AAACTTCTCA AGAACAACCA ACCCAACAAG ATTATCCTAG TATTGATGCA GGTCTTCCTA AGATAGAAGT TGATGACCAA CCAAAAGCAG CTCAACACAC TACTCTAGAA ACTGAAAGTG AACCTGATGT CTTTGAATTA AATGACAGTT TAAATCAACC CCAACAACCT ACAGAAAACT TGGGTGATGA CCAGTTTGTT GAAAAAGAAG TGCCACCTAC TCAACAGCTC CATCAGGATT TAGTCCACCA ACAACCCGTT CAAGTTGATA GTGGATCTCA AAACCATAGT TTTAATAATT CCCCTTCTTT AAAACCACCA CTAGTTAATA AACCAGCAAA GTTAGTTCAA CCTGAAGTAA AACATATTCC TCAAGTTGAA GTTCAACCGA AACCTCAAAT AGTTGAACCC AAAATTGAAC CAAAACCAGA AGTGAAACAT GTATCACATG TTGAAATTCA ACCAAAACCA GAAGTGAAAC CTGTTGTTGA TTCAGTACCA GAAGTTAAAC AACCTGAAGT AAAACATGTA CCACATGTTG AAGTTCAACC AAAACCTGTT GTTGATTTAA AACCTCAAAG GATTGAACCA AGAATTGAAT CAAAACCAGA AGTAATAAAA CATATTCCTC AAGTTGAAGT TCAACCGAAA GCTCAAATGG TTGAACCAAG AATTGAACCA AAACCTGAAA CAAAATACAT CCCTCAAGTA GAATCAACTC CTCAAGTTGA AGTTCACCAC TGAAAACCAG AAGTAAAAAC TGAATATCAA CCCCAACAAC CATTGCCAAC TTCAGGTTTA CAAATTAAAG TTGTACCAAG ATCAGCAGCT TTACTTCAAT CAAAATTAGA CACTGGTTTT CAACCTAGAC AAGTTGAACG CACAACTGAT TCAGATATCA CTGTTAGTGT CTCTTCCCAC GCTTCTCTTC TAGAGAAAAT TAATGCTTTA AACCACCAAA GGATAATGAG TGATATTGCT CTAAAGAGTG ATAACACTAT AAAAAGTAGC AATTTCAGCC GTTTTTATCC TGAAAATGAG TATGTTGCAA CTAAATACAG TGATCCACTT TACAGCGATA CCAATCAAAG TTTAACTAGT GATCGTTTTT CTCTTGATTT TGACTACACA CCAAAATCTA GAGTCAACAA TTACACACCA TTAAGATCAA CTAATTTCCA AAATAATGCT ATTTCTAACT ATCGTTTTAG CAGAACACCA AGTAGTTATT ATCCCTTAAC AAGAAGACCA TGAAGATTAA CTAATATCAG TTCATACCGT TCATCATTCC ATTCACCAAC AAGACTATCA AGTTTTAGAA GAACTAGTTT ACCATTTAGT TCTAGCTATG ATGGACTTAG ACGTTATCCA TCAAGATCAT ATTGATCTAA GGACTTT

Details for MygeA.20067.a.B2.GE42352

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	14kDa
OBSERVED MW:	16kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN DKQKAKINKA YNKLLKKINK RYPDVSVVYA RDHKNKVHAL YQDPESGNIF SLEKRKQLAS NYPLFELTSD NPISFTNNIV SLNAYDDKNN LVTVQYDQDN NTFYDQNGNV LDVS

Validated NT Sequence

atcttactca cttaagaaac atccaaaaca ttaccatttt ggtcatagaa agtgttgtta tcctgatcat attgaacagt taccaagttg ttcttatcat cataagcatt taagctaaca atgttatttg taaagctaat agggttatct gaagttaatt caaacaaagg atagttacta gctaactgct ttcttttttc taaagagaag atattacctg attcaggatc ttggtataga gcatgaacct tatttttatg atcacgggca taaacaacac taacatcagg atatcttttg ttaatctttt ttaaaagctt gttataggct ttgtttattt tagctttctg tttatcgttc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMN DKQKAKINKA YNKLLKKINK RYPDVSVVYA RDHKNKVHAL YQDPESGNIF SLEKRKQLAS NYPLFELTSD NPISFTNNIV SLNAYDDKNN LVTVQYDQDN NTFYDQNGNV LDVS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg ataaacagaa agctaaaata aacaaagcct ataacaagct tttaaaaaag attaacaaaa gatatcctga tgttagtgtt gtttatgccc gtgatcataa aaataaggtt catgctctat accaagatcc tgaatcaggt aatatcttct ctttagaaaa aagaaagcag ttagctagta actatccttt gtttgaatta acttcagata accctattag ctttacaaat aacattgtta gcttaaatgc ttatgatgat aagaacaact tggtaactgt tcaatatgat caggataaca acactttcta tgaccaaaat ggtaatgttt tggatgtttc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for MygeA.20067.a.B2.PW38535

PURIFICATION DATe:	10/2/2018
CONCENTRATION:	11.63mg/ml
OBSERVED MW:	18kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMN DKQKAKINKA YNKLLKKINK RYPDVSVVYA RDHKNKVHAL YQDPESGNIF SLEKRKQLAS NYPLFELTSD NPISFTNNIV SLNAYDDKNN LVTVQYDQDN NTFYDQNGNV LDVS

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMN DKQKAKINKA YNKLLKKINK RYPDVSVVYA RDHKNKVHAL YQDPESGNIF SLEKRKQLAS NYPLFELTSD NPISFTNNIV SLNAYDDKNN LVTVQYDQDN NTFYDQNGNV LDVS

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG ATAAACAGAA AGCTAAAATA AACAAAGCCT ATAACAAGCT TTTAAAAAAG ATTAACAAAA GATATCCTGA TGTTAGTGTT GTTTATGCCC GTGATCATAA AAATAAGGTT CATGCTCTAT ACCAAGATCC TGAATCAGGT AATATCTTCT CTTTAGAAAA AAGAAAGCAG TTAGCTAGTA ACTATCCTTT GTTTGAATTA ACTTCAGATA ACCCTATTAG CTTTACAAAT AACATTGTTA GCTTAAATGC TTATGATGAT AAGAACAACT TGGTAACTGT TCAATATGAT CAGGATAACA ACACTTTCTA TGACCAAAAT GGTAATGTTT TGGATGTTTC TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT