MygeA.19975.a

MgpA Bifunctional oligoribonuclease and PAP phosphatase NrnA (MG190) (MG_190)

CENTER ID:	MygeA.19975.a
ORGANISM:	Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	C

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MygeA.19975.a.B2.GE42348	DDH domain		18	160

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_81202

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|243273.25.peg.218
UniProt:	P22746

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKGSITEAI NAIKQFDKIV IFHHVRPDGD CLGAQQGLFH LIKANFKNKE VKCVGNNNNL FSFINMTFTN QIDESFLKEA LAIVVDANYK NRIELRELLD KNLFKAVLRI DHHPNEDDLN TSFNFVEESY VACCEQIVEM ATVAKWTIPP VAATLLYIGI YTDSNRFLYS NTSYRTLYLA AILYKAKADI RIVHDHLNHT SLADLKFKKY VYNHFKTQGQ VIYFICTKKI QKRLRMTADQ CARVNLLSNI ADYKIWLFFI EQANNEIRID LRSNGINVRD IAIKYGGGGH NNASGAIITN KKQISDVVSD CVKKIVYN

NT Sequence

ATGAAAAAAG GATCAATAAC TGAAGCAATT AATGCCATTA AACAATTTGA TAAGATTGTT ATCTTTCACC ATGTGCGCCC TGATGGGGAT TGTTTAGGAG CACAACAAGG CTTGTTTCAC CTCATTAAAG CTAACTTTAA AAATAAGGAG GTGAAGTGTG TTGGTAATAA CAACAACCTG TTTAGCTTTA TCAACATGAC ATTTACCAAC CAAATTGATG AGAGCTTTTT AAAAGAAGCA CTTGCCATTG TGGTCGATGC TAATTACAAA AACAGGATTG AATTGAGAGA ACTGTTAGAT AAAAACCTGT TTAAAGCAGT GTTAAGGATT GATCACCATC CCAATGAAGA TGATCTAAAC ACTAGCTTTA ACTTTGTTGA AGAAAGCTAT GTAGCTTGTT GTGAGCAGAT AGTGGAGATG GCCACAGTGG CGAAGTGGAC CATACCACCA GTGGCTGCTA CTTTACTATA TATAGGTATC TATACGGATA GTAATAGATT TCTATATAGT AATACATCAT ATAGAACACT ATACTTAGCA GCAATACTAT ATAAAGCTAA AGCTGATATA AGGATAGTAC ATGATCATTT AAACCATACT AGTTTAGCAG ATCTTAAGTT TAAAAAGTAT GTTTATAACC ACTTTAAAAC CCAAGGACAA GTGATCTATT TTATCTGTAC TAAAAAGATC CAAAAGAGAC TAAGAATGAC TGCAGATCAA TGTGCTAGAG TTAACTTGTT AAGTAACATA GCAGATTACA AGATCTGACT TTTCTTTATT GAACAAGCTA ATAATGAGAT CAGGATAGAA CTGAGGAGTA ATGGGATTAA TGTCAGAGAT ATAGCCATTA AGTATGGTGG GGGAGGACAT AATAATGCAA GTGGAGCGAT CATTACTAAC AAAAAACAAA TTAGTGATGT TGTTAGTGAT TGTGTGAAAA AAATTGTTTA TAAT

Details for MygeA.19975.a.B2.GE42348

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/18/2018
EXPECTED MW:	17kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK IVIFHHVRPD GDCLGAQQGL FHLIKANFKN KEVKCVGNNN NLFSFINMTF TNQIDESFLK EALAIVVDAN YKNRIELREL LDKNLFKAVL RIDHHPNEDD LNTSFNFVEE SYVACCEQIV EMATVAKWTI PPVAATLLYI GI

Validated NT Sequence

atcttactca cttagatacc tatatatagt aaagtagcag ccactggtgg tatggtccac ttcgccactg tggccatctc cactatctgc tcacaacaag ctacatagct ttcttcaaca aagttaaagc tagtgtttag atcatcttca ttgggatggt gatcaatcct taacactgct ttaaacaggt ttttatctaa cagttctctc aattcaatcc tgtttttgta attagcatcg accacaatgg caagtgcttc ttttaaaaag ctctcatcaa tttggttggt aaatgtcatg ttgataaagc taaacaggtt gttgttatta ccaacacact tcacctcctt atttttaaag ttagctttaa tgaggtgaaa caagccttgt tgtgctccta aacaatcccc atcagggcgc acatggtgaa agataacaat cttcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHKI VIFHHVRPDG DCLGAQQGLF HLIKANFKNK EVKCVGNNNN LFSFINMTFT NQIDESFLKE ALAIVVDANY KNRIELRELL DKNLFKAVLR IDHHPNEDDL NTSFNFVEES YVACCEQIVE MATVAKWTIP PVAATLLYIG I

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagattg ttatctttca ccatgtgcgc cctgatgggg attgtttagg agcacaacaa ggcttgtttc acctcattaa agctaacttt aaaaataagg aggtgaagtg tgttggtaat aacaacaacc tgtttagctt tatcaacatg acatttacca accaaattga tgagagcttt ttaaaagaag cacttgccat tgtggtcgat gctaattaca aaaacaggat tgaattgaga gaactgttag ataaaaacct gtttaaagca gtgttaagga ttgatcacca tcccaatgaa gatgatctaa acactagctt taactttgtt gaagaaagct atgtagcttg ttgtgagcag atagtggaga tggccacagt ggcgaagtgg accataccac cagtggctgc tactttacta tatataggta tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat