MygeA.19938.b

Uncharacterized protein MG096 (MG_096)

CENTER ID: MygeA.19938.b
ORGANISM: Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MygeA.19938.b.B3.GE42347 N-term domain DUF240 188 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243273.25.peg.106
UniProt: P47342

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTQKNIFKV KALLLSLFSA AVTITIFALP IFANNGSKTD LGLLSKNSAD FLGSSKRSLA GFDTPFSPDN LQYLEKETDY DQNFKSFTEK FKDEKITNNQ LGIVDIYNLF SGFHKSVKST VDLMNQLQKQ VEAANAIFPV DDAFVKLPKV PTELFKLVDD NVFPKLNPKG LNISDNIAAL FERYNLKSIE LKNFDLAFLR KADVIIKDKV RYNFEMQMQF QTVYVGGGNT VINLDFTLKA QTVNFANLQD LQNTFVKVGN DLSTQLFWIP TVNKLTDNAG NDLTHIAKTV IGESFFQTNV NLAKSVIEYD KVQPLVKQAF EERVLTPFKK EREAAKKAYE EEQRRLEEER KRQLEELRRR EAEEKRKAEE AKRNQEKARR EREAYEKSFN SFKDFKFYWL TKGKDVTKKA DLIDALKTAI ATPAYRNRTF SLLIKGFASG VERYFNANKN DKELKKLAFG EKGIQFPRAD AGVNGLYMSN FLRHELTSKA KFSLNLKDIK VENTVEDTQL YWKDNGIHLK QANPYKFNLN IKIKYNGWYN VHWWNWLPAK ILGIPTDWSG EMNLTFVVNG DLSEIVDKHD YPGTFFQFTD KNELLFTLAV REQIKVDNNH FMGLLKSQNL HNLQLASGAT KPPVVDLASY FHFVLLTEKS
NT Sequence
TTGAAAACAC AAAAAAATAT CTTTAAGGTC AAGGCCTTAC TACTTTCACT TTTTTCTGCT GCTGTTACCA TCACTATTTT TGCTTTACCT ATCTTTGCAA ACAATGGTTC TAAAACTGAT TTAGGTTTGC TTTCTAAAAA TAGTGCTGAT TTTTTAGGTA GTTCAAAAAG AAGTCTTGCT GGCTTTGATA CTCCTTTTAG TCCAGATAAC CTCCAGTATT TAGAAAAAGA AACTGATTAT GATCAGAACT TTAAAAGTTT TACTGAAAAG TTTAAAGATG AAAAAATAAC TAACAACCAA CTTGGTATTG TTGATATCTA TAACTTATTC AGTGGTTTTC ACAAGAGTGT CAAAAGCACA GTTGACTTAA TGAACCAACT GCAAAAGCAA GTTGAAGCTG CTAATGCTAT CTTCCCAGTT GATGATGCTT TTGTTAAATT ACCTAAAGTA CCTACTGAAT TATTTAAGTT AGTTGATGAT AATGTCTTTC CTAAGTTAAA TCCTAAGGGC TTAAATATCT CTGATAATAT TGCTGCACTT TTTGAAAGAT ATAACCTTAA ATCGATTGAA CTTAAGAACT TTGACTTAGC TTTCTTGAGA AAAGCTGATG TAATTATCAA AGACAAGGTT CGATATAACT TTGAGATGCA AATGCAGTTT CAAACTGTTT ATGTTGGTGG AGGGAATACC GTTATTAACT TAGACTTTAC TTTAAAAGCC CAAACCGTTA ACTTTGCTAA CCTCCAAGAT TTACAAAACA CTTTTGTTAA AGTTGGTAAT GATCTTTCCA CCCAACTCTT TTGGATTCCA ACTGTTAATA AATTAACTGA TAATGCAGGT AATGATCTTA CCCATATTGC TAAAACTGTG ATTGGTGAAT CGTTTTTCCA AACCAATGTT AACTTAGCTA AATCAGTTAT TGAATATGAT AAGGTTCAAC CATTGGTTAA ACAAGCTTTT GAAGAGCGAG TTTTAACTCC TTTCAAAAAG GAAAGAGAAG CTGCTAAAAA AGCTTATGAA GAAGAACAAC GTCGCTTGGA AGAGGAACGT AAGCGTCAAC TAGAAGAGCT AAGGAGAAGA GAAGCTGAGG AGAAAAGAAA AGCTGAAGAA GCAAAACGAA ATCAAGAAAA AGCACGCAGA GAAAGAGAAG CTTATGAAAA ATCATTTAAC TCCTTTAAAG ACTTTAAATT TTACTGGTTA ACTAAAGGTA AAGATGTTAC TAAAAAAGCT GATTTAATTG ATGCACTTAA AACCGCTATT GCTACACCAG CATACAGAAA TAGAACATTC TCTTTATTAA TCAAAGGTTT TGCTAGTGGA GTTGAACGTT ATTTCAACGC TAACAAGAAT GATAAAGAGT TGAAAAAACT TGCATTTGGT GAAAAAGGGA TCCAATTCCC TAGAGCAGAT GCTGGGGTAA ATGGTCTTTA CATGAGTAAT TTTTTGAGGC ATGAATTGAC AAGCAAAGCA AAATTTTCAC TTAACCTAAA AGATATAAAA GTTGAAAATA CGGTTGAAGA TACTCAATTG TATTGGAAGG ATAATGGCAT TCATTTAAAG CAAGCCAATC CTTATAAATT CAATCTAAAT ATCAAGATCA AGTACAACGG CTGGTATAAT GTCCACTGGT GAAACTGGCT CCCTGCTAAA ATTTTAGGGA TTCCTACAGA CTGAAGTGGT GAGATGAATT TAACATTTGT TGTCAACGGT GACCTATCAG AAATTGTTGA TAAACATGAT TACCCTGGTA CTTTCTTTCA ATTTACCGAT AAAAATGAAT TGCTATTTAC CTTAGCAGTT AGAGAACAAA TTAAGGTTGA TAATAATCAT TTTATGGGTC TGTTAAAAAG CCAAAACCTT CATAATCTTC AGCTTGCTTC TGGTGCTACA AAACCTCCTG TTGTTGATTT AGCTAGTTAT TTCCACTTTG TATTATTGAC TGAAAAATCT
Details for MygeA.19938.b.B3.GE42347
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS IELKNFDLAF LRKADVIIKD KVRYNFEMQM QFQTVYVGGG NTVINLDFTL KAQTVNFANL QDLQNTFVKV GNDLSTQLFW IPTVNKLTDN AGNDLTHIAK TVIGESFFQT NVNLAKSVIE YDKVQPLVKQ AFEERVLT
Validated NT Sequence
atcttactca cttaagttaa aactcgctct tcaaaagctt gtttaaccaa tggttgaacc ttatcatatt caataactga tttagctaag ttaacattgg tttggaaaaa cgattcacca atcacagttt tagcaatatg ggtaagatca ttacctgcat tatcagttaa tttattaaca gttggaatcc aaaagagttg ggtggaaaga tcattaccaa ctttaacaaa agtgttttgt aaatcttgga ggttagcaaa gttaacggtt tgggctttta aagtaaagtc taagttaata acggtattcc ctccaccaac ataaacagtt tgaaactgca tttgcatctc aaagttatat cgaaccttgt ctttgataat tacatcagct tttctcaaga aagctaagtc aaagttctta agttcaatcg acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHSI ELKNFDLAFL RKADVIIKDK VRYNFEMQMQ FQTVYVGGGN TVINLDFTLK AQTVNFANLQ DLQNTFVKVG NDLSTQLFWI PTVNKLTDNA GNDLTHIAKT VIGESFFQTN VNLAKSVIEY DKVQPLVKQA FEERVLT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgattg aacttaagaa ctttgactta gctttcttga gaaaagctga tgtaattatc aaagacaagg ttcgatataa ctttgagatg caaatgcagt ttcaaactgt ttatgttggt ggagggaata ccgttattaa cttagacttt actttaaaag cccaaaccgt taactttgct aacctccaag atttacaaaa cacttttgtt aaagttggta atgatctttc cacccaactc ttttggattc caactgttaa taaattaact gataatgcag gtaatgatct tacccatatt gctaaaactg tgattggtga atcgtttttc caaaccaatg ttaacttagc taaatcagtt attgaatatg ataaggttca accattggtt aaacaagctt ttgaagagcg agttttaact tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat