MygeA.19228.b

Uncharacterized RNA pseudouridine synthase MG209 (MG_209)

CENTER ID:	MygeA.19228.b
ORGANISM:	Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MygeA.19228.b.B1.GE42345	Full length(MygeA.19228.b)		1	308

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MygeA.19228.b.B1.PW38487	Full length(MygeA.19228.b)		1	308

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_81193

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|243273.25.peg.241
UniProt:	P47451

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQCFVVTTT KRLDSLLASL LNLSRVKVVK LIMNGQIKVN EKLTFKNSLI VAKDDVIKVE IHDETTSDFI TSVEPYNLKL EVLFEDKDLM VINKPSGLLT HPTTFNEKAS LLAACIFHNN KNPVYLVHRL DRDTSGAIVV CKNQASLLNL QNQLQNRTLK RYYVALVHFP FNALTGSINA PLARVNNNKV MFKIAQTAKA KQAITKFKVI NQNEKAALIS LELLTGRTHQ IRVHLKFIQH PVYNDPLYGI KSEKKDSYGQ FLHANRICFI HPTLNKPMDF HAPLEPKFST KLKSLNLSLT DPLHVLFK

NT Sequence

ATGAAACAGT GTTTTGTTGT TACAACTACC AAACGCTTAG ATAGTCTTTT AGCTAGCTTA CTGAACCTTT CAAGAGTAAA GGTAGTGAAG CTGATCATGA ATGGACAGAT TAAAGTTAAT GAAAAACTAA CTTTTAAAAA CAGTTTAATA GTTGCAAAAG ATGATGTAAT TAAAGTTGAG ATTCATGATG AGACAACTAG TGATTTCATT ACTAGTGTTG AACCTTATAA CTTAAAGCTT GAGGTTCTTT TTGAAGACAA GGATTTGATG GTTATTAACA AACCATCAGG TTTGTTAACC CATCCCACCA CTTTCAATGA AAAAGCCAGC TTGTTAGCTG CTTGTATCTT TCACAACAAC AAAAACCCTG TTTACTTAGT GCACAGATTG GACCGTGATA CTAGTGGGGC AATTGTTGTC TGTAAAAACC AAGCAAGCTT ATTAAATTTG CAAAATCAAC TGCAAAATCG CACCTTAAAA CGTTATTATG TAGCACTAGT CCACTTCCCT TTTAATGCCT TAACTGGTTC AATTAATGCA CCTTTAGCAA GGGTTAATAA CAACAAGGTA ATGTTTAAAA TAGCCCAAAC TGCTAAAGCA AAGCAAGCAA TAACTAAGTT TAAAGTGATT AATCAGAATG AAAAAGCAGC ACTAATTAGC TTGGAATTGT TAACAGGTAG AACCCACCAA ATTAGAGTGC ATCTGAAATT TATCCAACAT CCAGTTTATA ATGATCCACT GTATGGAATT AAAAGTGAAA AGAAAGATAG CTATGGTCAG TTTCTCCATG CAAACAGGAT CTGTTTTATC CATCCCACTT TAAACAAACC TATGGACTTT CACGCCCCAC TTGAACCTAA GTTTTCAACG AAACTTAAGA GTTTAAACTT ATCTTTAACC GATCCACTCC ATGTTCTTTT TAAG

Details for MygeA.19228.b.B1.GE42345

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK QCFVVTTTKR LDSLLASLLN LSRVKVVKLI MNGQIKVNEK LTFKNSLIVA KDDVIKVEIH DETTSDFITS VEPYNLKLEV LFEDKDLMVI NKPSGLLTHP TTFNEKASLL AACIFHNNKN PVYLVHRLDR DTSGAIVVCK NQASLLNLQN QLQNRTLKRY YVALVHFPFN ALTGSINAPL ARVNNNKVMF KIAQTAKAKQ AITKFKVINQ NEKAALISLE LLTGRTHQIR VHLKFIQHPV YNDPLYGIKS EKKDSYGQFL HANRICFIHP TLNKPMDFHA PLEPKFSTKL KSLNLSLTXX XHVLFK

Validated NT Sequence

atcttactca cttacttaaa aagaacatgg nnnnnnnngg ttaaagataa gtttaaactc ttaagtttcg ttgaaaactt aggttcaagt ggggcgtgaa agtccatagg tttgtttaaa gtgggatgga taaaacagat cctgtttgca tggagaaact gaccatagct atctttcttt tcacttttaa ttccatacag tggatcatta taaactggat gttggataaa tttcagatgc actctaattt ggtgggttct acctgttaac aattccaagc taattagtgc tgctttttca ttctgattaa tcactttaaa cttagttatt gcttgctttg ctttagcagt ttgggctatt ttaaacatta ccttgttgtt attaaccctt gctaaaggtg cattaattga accagttaag gcattaaaag ggaagtggac tagtgctaca taataacgtt ttaaggtgcg attttgcagt tgattttgca aatttaataa gcttgcttgg tttttacaga caacaattgc cccactagta tcacggtcca atctgtgcac taagtaaaca gggtttttgt tgttgtgaaa gatacaagca gctaacaagc tggctttttc attgaaagtg gtgggatggg ttaacaaacc tgatggtttg ttaataacca tcaaatcctt gtcttcaaaa agaacctcaa gctttaagtt ataaggttca acactagtaa tgaaatcact agttgtctca tcatgaatct caactttaat tacatcatct tttgcaacta ttaaactgtt tttaaaagtt agtttttcat taactttaat ctgtccattc atgatcagct tcactacctt tactcttgaa aggttcagta agctagctaa aagactatct aagcgtttgg tagttgtaac aacaaaacac tgtttcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac agtgttttgt tgttacaact accaaacgct tagatagtct tttagctagc ttactgaacc tttcaagagt aaaggtagtg aagctgatca tgaatggaca gattaaagtt aatgaaaaac taacttttaa aaacagttta atagttgcaa aagatgatgt aattaaagtt gagattcatg atgagacaac tagtgatttc attactagtg ttgaacctta taacttaaag cttgaggttc tttttgaaga caaggatttg atggttatta acaaaccatc aggtttgtta acccatccca ccactttcaa tgaaaaagcc agcttgttag ctgcttgtat ctttcacaac aacaaaaacc ctgtttactt agtgcacaga ttggaccgtg atactagtgg ggcaattgtt gtctgtaaaa accaagcaag cttattaaat ttgcaaaatc aactgcaaaa tcgcacctta aaacgttatt atgtagcact agtccacttc ccttttaatg ccttaactgg ttcaattaat gcacctttag caagggttaa taacaacaag gtaatgttta aaatagccca aactgctaaa gcaaagcaag caataactaa gtttaaagtg attaatcaga atgaaaaagc agcactaatt agcttggaat tgttaacagg tagaacccac caaattagag tgcatctgaa atttatccaa catccagttt ataatgatcc actgtatgga attaaaagtg aaaagaaaga tagctatggt cagtttctcc atgcaaacag gatctgtttt atccatccca ctttaaacaa acctatggac tttcacgccc cacttgaacc taagttttca acgaaactta agagtttaaa cttatcttta accgatccac tccatgttct ttttaagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for MygeA.19228.b.B1.PW38487

PURIFICATION DATe:	6/4/2018
CONCENTRATION:	11.33mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC AGTGTTTTGT TGTTACAACT ACCAAACGCT TAGATAGTCT TTTAGCTAGC TTACTGAACC TTTCAAGAGT AAAGGTAGTG AAGCTGATCA TGAATGGACA GATTAAAGTT AATGAAAAAC TAACTTTTAA AAACAGTTTA ATAGTTGCAA AAGATGATGT AATTAAAGTT GAGATTCATG ATGAGACAAC TAGTGATTTC ATTACTAGTG TTGAACCTTA TAACTTAAAG CTTGAGGTTC TTTTTGAAGA CAAGGATTTG ATGGTTATTA ACAAACCATC AGGTTTGTTA ACCCATCCCA CCACTTTCAA TGAAAAAGCC AGCTTGTTAG CTGCTTGTAT CTTTCACAAC AACAAAAACC CTGTTTACTT AGTGCACAGA TTGGACCGTG ATACTAGTGG GGCAATTGTT GTCTGTAAAA ACCAAGCAAG CTTATTAAAT TTGCAAAATC AACTGCAAAA TCGCACCTTA AAACGTTATT ATGTAGCACT AGTCCACTTC CCTTTTAATG CCTTAACTGG TTCAATTAAT GCACCTTTAG CAAGGGTTAA TAACAACAAG GTAATGTTTA AAATAGCCCA AACTGCTAAA GCAAAGCAAG CAATAACTAA GTTTAAAGTG ATTAATCAGA ATGAAAAAGC AGCACTAATT AGCTTGGAAT TGTTAACAGG TAGAACCCAC CAAATTAGAG TGCATCTGAA ATTTATCCAA CATCCAGTTT ATAATGATCC ACTGTATGGA ATTAAAAGTG AAAAGAAAGA TAGCTATGGT CAGTTTCTCC ATGCAAACAG GATCTGTTTT ATCCATCCCA CTTTAAACAA ACCTATGGAC TTTCACGCCC CACTTGAACC TAAGTTTTCA ACGAAACTTA AGAGTTTAAA CTTATCTTTA ACCGATCCAC TCCATGTTCT TTTTAAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT