MygeA.00914.a
Glyceraldehyde-3-phosphate dehydrogenase (MG_301)
| CENTER ID: | MygeA.00914.a |
| ORGANISM: | Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MygeA.00914.a.A11.GE43341 | Full length(MygeA.00914.a) | 1 | 337 | ||
| MygeA.00914.a.B1.GE42342 | Full length(MygeA.00914.a) | 1 | 337 | ||
| MygeA.00914.a.B2.GE43067 | XtalPred domain | 6 | 337 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MygeA.00914.a.A11.PW38712 | Full length(MygeA.00914.a) | 1 | 337 |
Structures
7JWK
DEPOSITED: 8/25/2020
DETERMINATION: XRay
CLONE: MygeA.00914.a.B2.GE43067
PROTEIN: MygeA.00914.a.B2.PW38693
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_81180 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|243273.25.peg.352 |
| UniProt: | P47543 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAKNRTIKV AINGFGRIGR LVFRSLLSKA NVEVVAINDL TQPEVLAHLL KYDSAHGELK RKITVKQNIL QIDRKKVYVF SEKDPQNLPW DEHDIDVVIE STGRFVSEEG ASLHLKAGAK RVIISAPAKE KTIRTVVYNV NHKTISSDDK IISAASCTTN CLAPLVHVLE KNFGIVYGTM LTVHAYTADQ RLQDAPHNDL RRARAAAVNI VPTTTGAAKA IGLVVPEANG KLNGMSLRVP VLTGSIVELS VVLEKSPSVE QVNQAMKRFA SASFKYCEDP IVSSDVVSSE YGSIFDSKLT NIVEVDGMKL YKVYAWYDNE SSYVHQLVRV VSYCAKL
NT Sequence
ATGGCAGCAA AGAATAGAAC CATTAAGGTT GCAATCAATG GTTTTGGAAG AATTGGAAGA CTTGTTTTTC GTTCTCTTCT CAGTAAGGCA AATGTTGAAG TTGTAGCAAT TAATGATTTG ACCCAACCTG AAGTTTTAGC GCACCTGTTG AAATATGATT CAGCTCATGG TGAATTGAAA AGAAAGATTA CTGTTAAACA AAACATCTTG CAAATTGATA GAAAAAAGGT TTATGTTTTT AGTGAAAAAG ATCCCCAAAA TTTACCTTGG GATGAACATG ATATTGATGT AGTAATTGAA TCAACTGGTA GGTTTGTAAG TGAAGAGGGT GCTTCTCTCC ATTTAAAAGC AGGTGCTAAA AGAGTAATTA TTTCCGCACC CGCTAAAGAA AAAACTATCA GGACAGTTGT TTACAATGTT AATCACAAAA CCATTAGTAG CGATGATAAG ATCATCTCAG CAGCTAGCTG TACTACTAAC TGTTTAGCAC CATTAGTTCA TGTACTTGAA AAGAACTTTG GGATTGTTTA TGGAACGATG CTAACAGTTC ATGCATATAC TGCAGATCAA CGCTTACAAG ATGCTCCTCA TAATGACTTA CGTCGTGCTC GTGCTGCAGC TGTTAACATT GTGCCAACAA CAACAGGAGC AGCTAAAGCA ATTGGGCTTG TTGTTCCAGA AGCAAATGGC AAACTTAATG GGATGTCACT CCGTGTTCCA GTGTTAACTG GTTCTATTGT AGAGTTAAGT GTTGTACTTG AAAAAAGTCC ATCTGTTGAA CAAGTAAATC AAGCCATGAA GCGATTTGCT TCCGCTTCTT TTAAATATTG TGAAGATCCT ATTGTATCTA GCGATGTGGT AAGTTCTGAA TATGGTTCAA TTTTCGATTC TAAACTAACC AATATTGTTG AAGTTGATGG CATGAAACTT TATAAGGTGT ATGCATGGTA TGATAATGAA TCTTCCTATG TACACCAACT AGTGAGAGTA GTTAGCTATT GTGCTAAGCT C
Details for MygeA.00914.a.A11.GE43341
| HARVESTED ON: | 7/31/2019 |
| SEQUENCED ON: | 8/7/2019 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKNRTIK VAINGFGRIG RLVFRSLLSK ANVEVVAIND LTQPEVLAHL LKYDSAHGEL KRKITVKQNI LQIDRKKVYV FSEKDPQNLP WDEHDIDVVI ESTGRFVSEE GASLHLKAGA KRVIISAPAK EKTIRTVVYN VNHKTISSDD KIISAASCTT NCLAPLVHVL EKNFGIVYGT MLTVHAYTAD QRLQDAPHND LRRARAAAVN IVPTTTGAAK AIGLVVPEAN GKLNGMSLRV PVLTGSIVEL SVVLEKSPSV EQVNQAMKRF ASASFKYAED PIVSSDVVSS EYGSIFDSKL TNIVEVDGMK LYKVYAWYDN ESSYVHQLVR VVSYC
Validated NT Sequence
tgcacaatag ctaactactc tcactagttg gtgtacatag gaagattcat tatcatacca tgcatacacc ttataaagtt tcatgccatc aacttcaaca atattggtta gtttagaatc gaaaattgaa ccatattcag aacttaccac atcgctagat acaataggat cttcagcata tttaaaagaa gcggaagcaa atcgcttcat ggcttgattt acttgttcaa cagatggact tttttcaagt acaacactta actctacaat agaaccagtt aacactggaa cacggagtga catcccatta agtttgccat ttgcttctgg aacaacaagc ccaattgctt tagctgctcc tgttgttgtt ggcacaatgt taacagctgc agcacgagca cgacgtaagt cattatgagg agcatcttgt aagcgttgat ctgcagtata tgcatgaact gttagcatcg ttccataaac aatcccaaag ttcttttcaa gtacatgaac taatggtgct aaacagttag tagtacagct agctgctgag atgatcttat catcgctact aatggttttg tgattaacat tgtaaacaac tgtcctgata gttttttctt tagcgggtgc ggaaataatt actcttttag cacctgcttt taaatggaga gaagcaccct cttcacttac aaacctacca gttgattcaa ttactacatc aatatcatgt tcatcccaag gtaaattttg gggatctttt tcactaaaaa cataaacctt ttttctatca atttgcaaga tgttttgttt aacagtaatc tttcttttca attcaccatg agctgaatca tatttcaaca ggtgcgctaa aacttcaggt tgggtcaaat cattaattgc tacaacttca acatttgcct tactgagaag agaacgaaaa acaagtcttc caattcttcc aaaaccattg attgcaacct taatggttct attctttgct gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKNRTIK VAINGFGRIG RLVFRSLLSK ANVEVVAIND LTQPEVLAHL LKYDSAHGEL KRKITVKQNI LQIDRKKVYV FSEKDPQNLP WDEHDIDVVI ESTGRFVSEE GASLHLKAGA KRVIISAPAK EKTIRTVVYN VNHKTISSDD KIISAASCTT NCLAPLVHVL EKNFGIVYGT MLTVHAYTAD QRLQDAPHND LRRARAAAVN IVPTTTGAAK AIGLVVPEAN GKLNGMSLRV PVLTGSIVEL SVVLEKSPSV EQVNQAMKRF ASASFKYAED PIVSSDVVSS EYGSIFDSKL TNIVEVDGMK LYKVYAWYDN ESSYVHQLVR VVSYCAKL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagcaaag aatagaacca ttaaggttgc aatcaatg
gttttggaag aattggaaga cttgtttttc gttctcttct cagtaaggca aatgttgaag ttgtagcaat
taatgatttg acccaacctg aagttttagc gcacctgttg aaatatgatt cagctcatgg tgaattgaaa
agaaagatta ctgttaaaca aaacatcttg caaattgata gaaaaaaggt ttatgttttt agtgaaaaag
atccccaaaa tttaccttgg gatgaacatg atattgatgt agtaattgaa tcaactggta ggtttgtaag
tgaagagggt gcttctctcc atttaaaagc aggtgctaaa agagtaatta tttccgcacc cgctaaagaa
aaaactatca ggacagttgt ttacaatgtt aatcacaaaa ccattagtag cgatgataag atcatctcag
cagctagctg tactactaac tgtttagcac cattagttca tgtacttgaa aagaactttg ggattgttta
tggaacgatg ctaacagttc atgcatatac tgcagatcaa cgcttacaag atgctcctca taatgactta
cgtcgtgctc gtgctgcagc tgttaacatt gtgccaacaa caacaggagc agctaaagca attgggcttg
ttgttccaga agcaaatggc aaacttaatg ggatgtcact ccgtgttcca gtgttaactg gttctattgt
agagttaagt gttgtacttg aaaaaagtcc atctgttgaa caagtaaatc aagccatgaa gcgatttgct
tccgcttctt ttaaatatgc tgaagatcct
attgtatcta gcgatgtggt aagttctgaa tatggttcaa ttttcgattc taaactaacc aatattgttg
aagttgatgg catgaaactt tataaggtgt atgcatggta tgataatgaa tcttcctatg tacaccaact
agtgagagta gttagctatt gtgctaagct ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MygeA.00914.a.B1.GE42342
| HARVESTED ON: | 4/13/2018 |
| SEQUENCED ON: | 4/18/2018 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MAAKNRTIKV AINGFGRIGR LVFRSLLSKA NVEVVAINDL TQPEVLAHLL KYDSAHGELK RKITVKQNIL QIDRKKVYVF SEKDPQNLPW DEHDIDVVIE STGRFVSEEG ASLHLKAGAK RVIISAPAKE KTIRTVVYNV NHKTISSDDK IISAASCTTN CLAPLVHVLE KNFGIVYGTM LTVHAYTADQ RLQDAPHNDL RRARAAAVNI VPTTTGAAKA IGLVVPEANG KLNGMSLRVP VLTGSIVELS VVLEKSPSVE QVNQAMKRFA SASFKYCEDP IVSSDVVSSE YGSIFDSKLT NIVEVDGMKL YKVYAWYDNE SSYVHQLX
Validated NT Sequence
tntnnctagt tggtgtacat aggaagattc attatcatac catgcataca ccttataaag tttcatgcca tcaacttcaa caatattggt tagtttagaa tcgaaaattg aaccatattc agaacttacc acatcgctag atacaatagg atcttcacaa tatttaaaag aagcggaagc aaatcgcttc atggcttgat ttacttgttc aacagatgga cttttttcaa gtacaacact taactctaca atagaaccag ttaacactgg aacacggagt gacatcccat taagtttgcc atttgcttct ggaacaacaa gcccaattgc tttagctgct cctgttgttg ttggcacaat gttaacagct gcagcacgag cacgacgtaa gtcattatga ggagcatctt gtaagcgttg atctgcagta tatgcatgaa ctgttagcat cgttccataa acaatcccaa agttcttttc aagtacatga actaatggtg ctaaacagtt agtagtacag ctagctgctg agatgatctt atcatcgcta ctaatggttt tgtgattaac attgtaaaca actgtcctga tagttttttc tttagcgggt gcggaaataa ttactctttt agcacctgct tttaaatgga gagaagcacc ctcttcactt acaaacctac cagttgattc aattactaca tcaatatcat gttcatccca aggtaaattt tggggatctt tttcactaaa aacataaacc ttttttctat caatttgcaa gatgttttgt ttaacagtaa tctttctttt caattcacca tgagctgaat catatttcaa caggtgcgct aaaacttcag gttgggtcaa atcattaatt gctacaactt caacatttgc cttactgaga agagaacgaa aaacaagtct tccaattctt ccaaaaccat tgattgcaac cttaatggtt ctattctttg ctgccatatg gtggtggt
Expected Protein Sequence
MAHHHHHHMA AKNRTIKVAI NGFGRIGRLV FRSLLSKANV EVVAINDLTQ PEVLAHLLKY DSAHGELKRK ITVKQNILQI DRKKVYVFSE KDPQNLPWDE HDIDVVIEST GRFVSEEGAS LHLKAGAKRV IISAPAKEKT IRTVVYNVNH KTISSDDKII SAASCTTNCL APLVHVLEKN FGIVYGTMLT VHAYTADQRL QDAPHNDLRR ARAAAVNIVP TTTGAAKAIG LVVPEANGKL NGMSLRVPVL TGSIVELSVV LEKSPSVEQV NQAMKRFASA SFKYCEDPIV SSDVVSSEYG SIFDSKLTNI VEVDGMKLYK VYAWYDNESS YVHQLVRVVS YCAKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag caaagaatag aaccattaag gttgcaatca atggttttgg aagaattgga agacttgttt ttcgttctct tctcagtaag gcaaatgttg aagttgtagc aattaatgat ttgacccaac ctgaagtttt agcgcacctg ttgaaatatg attcagctca tggtgaattg aaaagaaaga ttactgttaa acaaaacatc ttgcaaattg atagaaaaaa ggtttatgtt tttagtgaaa aagatcccca aaatttacct tgggatgaac atgatattga tgtagtaatt gaatcaactg gtaggtttgt aagtgaagag ggtgcttctc tccatttaaa agcaggtgct aaaagagtaa ttatttccgc acccgctaaa gaaaaaacta tcaggacagt tgtttacaat gttaatcaca aaaccattag tagcgatgat aagatcatct cagcagctag ctgtactact aactgtttag caccattagt tcatgtactt gaaaagaact ttgggattgt ttatggaacg atgctaacag ttcatgcata tactgcagat caacgcttac aagatgctcc tcataatgac ttacgtcgtg ctcgtgctgc agctgttaac attgtgccaa caacaacagg agcagctaaa gcaattgggc ttgttgttcc agaagcaaat ggcaaactta atgggatgtc actccgtgtt ccagtgttaa ctggttctat tgtagagtta agtgttgtac ttgaaaaaag tccatctgtt gaacaagtaa atcaagccat gaagcgattt gcttccgctt cttttaaata ttgtgaagat cctattgtat ctagcgatgt ggtaagttct gaatatggtt caattttcga ttctaaacta accaatattg ttgaagttga tggcatgaaa ctttataagg tgtatgcatg gtatgataat gaatcttcct atgtacacca actagtgaga gtagttagct attgtgctaa gctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MygeA.00914.a.B2.GE43067
| HARVESTED ON: | 4/19/2019 |
| SEQUENCED ON: | 4/23/2019 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMT TIKVAINGFG RIGRLVFRSL LSKANVEVVX INDLTQPEVL AHLLKYDSAH GELKRKITVK QNILQIDRKK VYVFSEKDPQ NLPWDEHDID VVIESTGRFV SEEGASLHLK AGAKRVIISA PAKEKTIRTV VYNVNHKTIS SDDKIISAAS CTTNCLAPLV HVLEKNFGIV YGTMLTVHAY TADQRLQDAP HNDLRRARAA AVNIVPTTTG AAKAIGLVVP EANGKLNGMS LRVPVLTGSI VELSVVLEKS PSVEQVNQAM KRFASASFKY CEDPIVSSDV VSSEYGSIFD SKLTNIVEVD GMKLYKVYAW YDNESSYVHQ L
Validated NT Sequence
accatggctc accaccacca ccaccatatg acaaccatta aggttgcaat caatggtttt ggaagaattg gaagacttgt ttttcgttct cttctcagta aggcaaatgt tgaagttgtn nnaattaatg atttgaccca acctgaagtt ttagcgcacc tgttgaaata tgattcagct catggtgaat tgaaaagaaa gattactgtt aaacaaaaca tcttgcaaat tgatagaaaa aaggtttatg tttttagtga aaaagatccc caaaatttac cttgggatga acatgatatt gatgtagtaa ttgaatcaac tggtaggttt gtaagtgaag agggtgcttc tctccattta aaagcaggtg ctaaaagagt aattatttcc gcacccgcta aagaaaaaac tatcaggaca gttgtttaca atgttaatca caaaaccatt agtagcgatg ataagatcat ctcagcagct agctgtacta ctaactgttt agcaccatta gttcatgtac ttgaaaagaa ctttgggatt gtttatggaa cgatgctaac agttcatgca tatactgcag atcaacgctt acaagatgct cctcataatg acttacgtcg tgctcgtgct gcagctgtta acattgtgcc aacaacaaca ggagcagcta aagcaattgg gcttgttgtt ccagaagcaa atggcaaact taatgggatg tcactccgtg ttccagtgtt aactggttct attgtagagt taagtgttgt acttgaaaaa agtccatctg ttgaacaagt aaatcaagcc atgaagcgat ttgcttccgc ttcttttaaa tattgtgaag atcctattgt atctagcgat gtggtaagtt ctgaatatgg ttcaattttc gattctaaac taaccaatat tgttgaagtt gatggcatga aactttataa ggtgtatgca tggtatgata atgaatcttc ctatgtacac caactag
Expected Protein Sequence
MAHHHHHHRT IKVAINGFGR IGRLVFRSLL SKANVEVVAI NDLTQPEVLA HLLKYDSAHG ELKRKITVKQ NILQIDRKKV YVFSEKDPQN LPWDEHDIDV VIESTGRFVS EEGASLHLKA GAKRVIISAP AKEKTIRTVV YNVNHKTISS DDKIISAASC TTNCLAPLVH VLEKNFGIVY GTMLTVHAYT ADQRLQDAPH NDLRRARAAA VNIVPTTTGA AKAIGLVVPE ANGKLNGMSL RVPVLTGSIV ELSVVLEKSP SVEQVNQAMK RFASASFKYC EDPIVSSDVV SSEYGSIFDS KLTNIVEVDG MKLYKVYAWY DNESSYVHQL VRVVSYCAKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagaacca ttaaggttgc aatcaatggt tttggaagaa ttggaagact tgtttttcgt tctcttctca gtaaggcaaa tgttgaagtt gtagcaatta atgatttgac ccaacctgaa gttttagcgc acctgttgaa atatgattca gctcatggtg aattgaaaag aaagattact gttaaacaaa acatcttgca aattgataga aaaaaggttt atgtttttag tgaaaaagat ccccaaaatt taccttggga tgaacatgat attgatgtag taattgaatc aactggtagg tttgtaagtg aagagggtgc ttctctccat ttaaaagcag gtgctaaaag agtaattatt tccgcacccg ctaaagaaaa aactatcagg acagttgttt acaatgttaa tcacaaaacc attagtagcg atgataagat catctcagca gctagctgta ctactaactg tttagcacca ttagttcatg tacttgaaaa gaactttggg attgtttatg gaacgatgct aacagttcat gcatatactg cagatcaacg cttacaagat gctcctcata atgacttacg tcgtgctcgt gctgcagctg ttaacattgt gccaacaaca acaggagcag ctaaagcaat tgggcttgtt gttccagaag caaatggcaa acttaatggg atgtcactcc gtgttccagt gttaactggt tctattgtag agttaagtgt tgtacttgaa aaaagtccat ctgttgaaca agtaaatcaa gccatgaagc gatttgcttc cgcttctttt aaatattgtg aagatcctat tgtatctagc gatgtggtaa gttctgaata tggttcaatt ttcgattcta aactaaccaa tattgttgaa gttgatggca tgaaacttta taaggtgtat gcatggtatg ataatgaatc ttcctatgta caccaactag tgagagtagt tagctattgt gctaagctct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for MygeA.00914.a.A11.PW38712
| PURIFICATION DATe: | 9/30/2019 |
| CONCENTRATION: | 14.6mg/ml |
| OBSERVED MW: | 41kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKNRTIK VAINGFGRIG RLVFRSLLSK ANVEVVAIND LTQPEVLAHL LKYDSAHGEL KRKITVKQNI LQIDRKKVYV FSEKDPQNLP WDEHDIDVVI ESTGRFVSEE GASLHLKAGA KRVIISAPAK EKTIRTVVYN VNHKTISSDD KIISAASCTT NCLAPLVHVL EKNFGIVYGT MLTVHAYTAD QRLQDAPHND LRRARAAAVN IVPTTTGAAK AIGLVVPEAN GKLNGMSLRV PVLTGSIVEL SVVLEKSPSV EQVNQAMKRF ASASFKYAED PIVSSDVVSS EYGSIFDSKL TNIVEVDGMK LYKVYAWYDN ESSYVHQLVR VVSYC
Validated NT Sequence
tgcacaatag ctaactactc tcactagttg gtgtacatag gaagattcat tatcatacca tgcatacacc ttataaagtt tcatgccatc aacttcaaca atattggtta gtttagaatc gaaaattgaa ccatattcag aacttaccac atcgctagat acaataggat cttcagcata tttaaaagaa gcggaagcaa atcgcttcat ggcttgattt acttgttcaa cagatggact tttttcaagt acaacactta actctacaat agaaccagtt aacactggaa cacggagtga catcccatta agtttgccat ttgcttctgg aacaacaagc ccaattgctt tagctgctcc tgttgttgtt ggcacaatgt taacagctgc agcacgagca cgacgtaagt cattatgagg agcatcttgt aagcgttgat ctgcagtata tgcatgaact gttagcatcg ttccataaac aatcccaaag ttcttttcaa gtacatgaac taatggtgct aaacagttag tagtacagct agctgctgag atgatcttat catcgctact aatggttttg tgattaacat tgtaaacaac tgtcctgata gttttttctt tagcgggtgc ggaaataatt actcttttag cacctgcttt taaatggaga gaagcaccct cttcacttac aaacctacca gttgattcaa ttactacatc aatatcatgt tcatcccaag gtaaattttg gggatctttt tcactaaaaa cataaacctt ttttctatca atttgcaaga tgttttgttt aacagtaatc tttcttttca attcaccatg agctgaatca tatttcaaca ggtgcgctaa aacttcaggt tgggtcaaat cattaattgc tacaacttca acatttgcct tactgagaag agaacgaaaa acaagtcttc caattcttcc aaaaccattg attgcaacct taatggttct attctttgct gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAKNRTIK VAINGFGRIG RLVFRSLLSK ANVEVVAIND LTQPEVLAHL LKYDSAHGEL KRKITVKQNI LQIDRKKVYV FSEKDPQNLP WDEHDIDVVI ESTGRFVSEE GASLHLKAGA KRVIISAPAK EKTIRTVVYN VNHKTISSDD KIISAASCTT NCLAPLVHVL EKNFGIVYGT MLTVHAYTAD QRLQDAPHND LRRARAAAVN IVPTTTGAAK AIGLVVPEAN GKLNGMSLRV PVLTGSIVEL SVVLEKSPSV EQVNQAMKRF ASASFKYAED PIVSSDVVSS EYGSIFDSKL TNIVEVDGMK LYKVYAWYDN ESSYVHQLVR VVSYCAKL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGCAAAG AATAGAACCA TTAAGGTTGC AATCAATG
GTTTTGGAAG AATTGGAAGA CTTGTTTTTC GTTCTCTTCT CAGTAAGGCA AATGTTGAAG TTGTAGCAAT
TAATGATTTG ACCCAACCTG AAGTTTTAGC GCACCTGTTG AAATATGATT CAGCTCATGG TGAATTGAAA
AGAAAGATTA CTGTTAAACA AAACATCTTG CAAATTGATA GAAAAAAGGT TTATGTTTTT AGTGAAAAAG
ATCCCCAAAA TTTACCTTGG GATGAACATG ATATTGATGT AGTAATTGAA TCAACTGGTA GGTTTGTAAG
TGAAGAGGGT GCTTCTCTCC ATTTAAAAGC AGGTGCTAAA AGAGTAATTA TTTCCGCACC CGCTAAAGAA
AAAACTATCA GGACAGTTGT TTACAATGTT AATCACAAAA CCATTAGTAG CGATGATAAG ATCATCTCAG
CAGCTAGCTG TACTACTAAC TGTTTAGCAC CATTAGTTCA TGTACTTGAA AAGAACTTTG GGATTGTTTA
TGGAACGATG CTAACAGTTC ATGCATATAC TGCAGATCAA CGCTTACAAG ATGCTCCTCA TAATGACTTA
CGTCGTGCTC GTGCTGCAGC TGTTAACATT GTGCCAACAA CAACAGGAGC AGCTAAAGCA ATTGGGCTTG
TTGTTCCAGA AGCAAATGGC AAACTTAATG GGATGTCACT CCGTGTTCCA GTGTTAACTG GTTCTATTGT
AGAGTTAAGT GTTGTACTTG AAAAAAGTCC ATCTGTTGAA CAAGTAAATC AAGCCATGAA GCGATTTGCT
TCCGCTTCTT TTAAATATGC TGAAGATCCT
ATTGTATCTA GCGATGTGGT AAGTTCTGAA TATGGTTCAA TTTTCGATTC TAAACTAACC AATATTGTTG
AAGTTGATGG CATGAAACTT TATAAGGTGT ATGCATGGTA TGATAATGAA TCTTCCTATG TACACCAACT
AGTGAGAGTA GTTAGCTATT GTGCTAAGCT CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA