MygeA.00430.a

Tryptophan--tRNA ligase (MG_126)

CENTER ID: MygeA.00430.a
ORGANISM: Mycoplasma genitalium ATCC 33530 / G-37 / NCTC 10195
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 C

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MygeA.00430.a.A11.GE43340 Full length(MygeA.00430.a) 1 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_81177
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|243273.25.peg.137
UniProt: P47372
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKRAITGIQ ASGRQHLGNF LGVMQGLKQL QSQYQLFLFV ADLHAITVDF EPTMLKDNNL QLVKTLLALG LDYGKVNLFL QSDLMEHTML GYLMLTQSNL GELQRMTQFK TKKLAQKRNS NNTITIPTGL LTYPVLMAAD ILLYQPDIVP VGNDQKQHLE LTNDLAKRVA KKFKLKLKLP VFIENKDTNR IMDLSNPLKK MSKSNPDQNG VIYLDDSKET IIKKVRKATT DSFNKIRFAK KTQPGVTNLL VILTALLKEE VNHNLSKKIG SDLVKYYQNK SYLDLKNDLS SAVINVIESL KFKKAQITDE MVLKVLNDGK NQAKKVADET LKMFYKAFGL TSNQLFD
NT Sequence
ATGATAAAGC GCGCAATTAC AGGGATTCAA GCTTCTGGAA GACAACACCT AGGTAACTTT CTTGGCGTAA TGCAAGGTTT AAAACAACTC CAAAGTCAAT ACCAACTGTT TTTATTTGTT GCTGATCTTC ATGCTATTAC TGTTGATTTT GAACCAACAA TGCTCAAAGA TAACAACTTG CAACTTGTTA AAACTTTATT AGCACTAGGA CTTGATTATG GAAAAGTGAA CTTATTTTTA CAAAGTGATC TGATGGAACA TACCATGTTA GGTTATCTAA TGCTGACACA AAGTAATCTA GGTGAATTAC AAAGAATGAC CCAATTTAAA ACAAAGAAAT TAGCGCAAAA AAGAAATAGT AATAACACCA TTACTATCCC AACTGGTTTG TTAACTTACC CAGTGTTAAT GGCTGCTGAT ATCTTGCTTT ATCAACCTGA TATTGTTCCA GTTGGTAATG ATCAGAAGCA GCACTTGGAA TTAACCAATG ATTTAGCTAA ACGTGTAGCA AAAAAATTTA AGTTAAAACT GAAATTACCT GTATTTATAG AAAACAAAGA TACCAACAGG ATCATGGATC TATCAAATCC TTTAAAAAAG ATGTCCAAAT CAAATCCTGA TCAAAATGGT GTTATCTATC TGGATGATAG TAAAGAAACA ATCATCAAAA AAGTGCGCAA AGCCACAACT GATAGTTTTA ATAAGATTCG TTTTGCTAAA AAAACCCAAC CTGGTGTTAC TAATTTACTT GTTATTTTAA CTGCACTTTT AAAAGAAGAA GTTAACCATA ATTTAAGTAA AAAAATCGGC TCTGATCTTG TTAAATATTA TCAGAATAAA AGTTATTTAG ATTTAAAGAA TGACCTCAGT AGTGCTGTTA TTAATGTCAT AGAATCACTT AAATTTAAAA AAGCACAAAT TACTGATGAA ATGGTATTAA AAGTCCTAAA TGATGGTAAA AACCAAGCTA AAAAAGTTGC TGATGAAACA TTAAAAATGT TTTATAAAGC ATTTGGTTTA ACATCTAATC AGCTTTTTGA T
Details for MygeA.00430.a.A11.GE43340
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIKRAITGI QASGRQHLGN FLGVMQGLKQ LQSQYQLFLF VADLHAITVD FEPTMLKDNN LQLVKTLLAL GLDYGKVNLF LQSDLMEHTM LGYLMLTQSN LGELQRMTQF KTKKLAQKRN SNNTITIPTG LLTYPVLMAA DILLYQPDIV PVGNDQKQHL ELTNDLAKRV AKKFKLKLKL PVFIENKDTN RIMDLSNPLK KMSKSNPDQN GVIYLDDSKE TIIKKVRKAT TDSFNKIRFA KKTQPGVTNL LVILTALLKE EVNHNLSKKI GSDLVKYYQN KSYLDLKNDL SSAVINVIES LKFKKAQITD EMVLKVLNDG KNQAKKVADE TLKMFYKAFG LTSNQLFD
Validated NT Sequence
taatcgaaca gttgattcga agtcaggccg aatgctttgt agaacatttt cagggtttca tccgcaactt tcttggcttg gtttttgccg tcgttcagca ctttcagcac catctcgtcg gtaatttgtg ccttcttaaa cttcaagctc tcaataacgt tgatcaccgc ggaagataaa tcattcttca gatccagata cgatttgttt tgatagtatt taaccaggtc agagccgatc tttttgctca ggttatgatt aacctcttcc ttcagcagcg ccgtcaggat gaccagtaag ttcgtgacac ccggttgggt tttcttggca aaacggattt tgttgaagct atcggtggtc gccttacgta cttttttgat aatcgtttct ttgctatcat ccaggtagat cacaccattc tgatccggat tgctttttga catctttttc agcgggttgc tcagatccat gatacgattg gtatccttat tctcgatgaa aaccggcagt ttcaatttca gcttaaattt ctttgcaacg cgctttgcca ggtcattcgt cagctccaga tgctgcttct gatcgttgcc cacaggtaca atgtccggct gatacagcag aatatccgcc gccatcagca ctggatacgt cagcagaccg gtcgggatgg taatggtatt attggaatta cgcttctgtg ccagtttttt ggttttaaac tgggtcatgc gctgcagctc acccaaattg gattgcgtca gcatcaggta gccaagcatc gtatgctcca tcagatcgga ttgaaggaac aggttaacct tgccatagtc cagacccaat gccagcaggg ttttcaccag ctgcagattg ttatctttca acatcgtcgg ctcgaagtcc accgtgatgg cgtgcagatc cgcgacgaac agaaataatt gatattggct ttgcagttgc ttcagaccct gcatcacacc cagaaaatta ccgaggtgtt ggcgaccgga cgcctgaata ccggtaatcg cgcgtttaat catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIKRAITGI QASGRQHLGN FLGVMQGLKQ LQSQYQLFLF VADLHAITVD FEPTMLKDNN LQLVKTLLAL GLDYGKVNLF LQSDLMEHTM LGYLMLTQSN LGELQRMTQF KTKKLAQKRN SNNTITIPTG LLTYPVLMAA DILLYQPDIV PVGNDQKQHL ELTNDLAKRV AKKFKLKLKL PVFIENKDTN RIMDLSNPLK KMSKSNPDQN GVIYLDDSKE TIIKKVRKAT TDSFNKIRFA KKTQPGVTNL LVILTALLKE EVNHNLSKKI GSDLVKYYQN KSYLDLKNDL SSAVINVIES LKFKKAQITD EMVLKVLNDG KNQAKKVADE TLKMFYKAFG LTSNQLFD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattaaacgc gcgattaccg gtattcaggc gtccggtcgc caacacctcg gtaattttct gggtgtgatg cagggtctga agcaactgca aagccaatat caattatttc tgttcgtcgc ggatctgcac gccatcacgg tggacttcga gccgacgatg ttgaaagata acaatctgca gctggtgaaa accctgctgg cattgggtct ggactatggc aaggttaacc tgttccttca atccgatctg atggagcata cgatgcttgg ctacctgatg ctgacgcaat ccaatttggg tgagctgcag cgcatgaccc agtttaaaac caaaaaactg gcacagaagc gtaattccaa taataccatt accatcccga ccggtctgct gacgtatcca gtgctgatgg cggcggatat tctgctgtat cagccggaca ttgtacctgt gggcaacgat cagaagcagc atctggagct gacgaatgac ctggcaaagc gcgttgcaaa gaaatttaag ctgaaattga aactgccggt tttcatcgag aataaggata ccaatcgtat catggatctg agcaacccgc tgaaaaagat gtcaaaaagc aatccggatc agaatggtgt gatctacctg gatgatagca aagaaacgat tatcaaaaaa gtacgtaagg cgaccaccga tagcttcaac aaaatccgtt ttgccaagaa aacccaaccg ggtgtcacga acttactggt catcctgacg gcgctgctga aggaagaggt taatcataac ctgagcaaaa agatcggctc tgacctggtt aaatactatc aaaacaaatc gtatctggat ctgaagaatg atttatcttc cgcggtgatc aacgttattg agagcttgaa gtttaagaag gcacaaatta ccgacgagat ggtgctgaaa gtgctgaacg acggcaaaaa ccaagccaag aaagttgcgg atgaaaccct gaaaatgttc tacaaagcat tcggcctgac ttcgaatcaa ctgttcgatt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa