MyfoA.19284.a

Ubiquinol-cytochrome C reductase iron-sulfur subunit

CENTER ID: MyfoA.19284.a
ORGANISM: Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyfoA.19284.a.B2.GE40534 Rieske iron-sulfur domain 207 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1214102.3.peg.4392
UniProt: K0UR03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRGTSMTDN TPKVPSDDEL ASMSREELVE LGGKIDGVET IYKEPRWPVP GTKAEKRTER LVAYWLLLGG LSGLALLLVF LFWPWEYQPF GSEGEFLYTL ATPLYGLTFG LSILSIGIGA VLFQKKFIPE EISVQDRHDG RSPELARKTI AANLTDALEG STVKRRKLIG LSLGIGLGAF GAGTLVAFIG GLIKNPWKPV VPTAEGKKAV LWTSGWTPRF HGETIYLARA TGRPGSSPFV KMRPEDLDAG GMETVFPWRE SDGDGTTVES EHHLTEIAMG VRNPVMLIRI KPADMPRVVK RKGQESFNFG ELFAYTKVCS HLGCPSSLYE QQTYRILCPC HQSQFDALHF AKPIFGPAAR ALAQLPITID KDGYLVANGD FVEPVGPAFW ERKS
NT Sequence
gtggatcggg gcacgagcat gaccgataac actccgaagg ttccctcgga cgacgagctg gcctcgatgt cgcgtgagga gctcgtcgag ctcggcggca agatcgacgg cgtcgagacc atctacaagg agccgcgctg gccggtcccc gggaccaagg ccgagaagcg caccgagcgg ctggtcgcgt actggcttct gctcggcggg ctttcgggcc tggcactgct cctggtcttc ctgttctggc cgtgggagta ccagcccttc gggtcggagg gcgagttcct ctacacgctg gccaccccgc tgtacggcct caccttcggc ctgtcgatcc tgtcgatcgg catcggcgcg gtgctgttcc agaagaagtt catccccgag gagatctcgg tccaggaccg ccacgacggc cgctcccccg agctggcccg caagaccatc gcggccaacc tgaccgacgc cctggagggt tcgaccgtca agcgccgcaa gctgatcggt ctgtcgctgg gcatcggcct cggcgccttc ggcgccggca ccctggtggc cttcatcggc gggctgatca agaacccgtg gaagcccgtg gtgcccaccg ccgaaggcaa gaaggccgtg ttgtggacct cggggtggac gccccgattc cacggcgaga ccatctacct ggcgcgggcg accgggcgtc ccggctcctc tccgttcgtc aagatgcggc ccgaggatct cgatgccggt ggcatggaga cggtcttccc ctggcgcgag tctgacggcg acggcaccac cgtcgagtcc gagcaccacc tcaccgagat cgcgatgggt gtgcgtaacc cggtcatgtt gatccgcatc aaaccggccg acatgccgcg tgtggtcaag cgaaagggtc aggagagctt caacttcggt gagctgttcg cctacaccaa ggtctgctcg cacctgggct gcccctcgtc cctgtacgag cagcagacct accgcatcct gtgcccgtgc caccagtcgc agttcgacgc gttgcacttc gcaaagccca ttttcggtcc ggctgcgcgc gcgttggcgc agctgcccat caccatcgac aaagacggct acctcgtcgc caacggcgac ttcgtcgaac ccgtcggacc ggcattctgg gagcgcaaat catga
Details for MyfoA.19284.a.B2.GE40534
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK KAVLWTSGWT PRFHGETIYL ARATGRPGSS PFVKMRPEDL DAGGMETVFP WRESDGDGTT VESEHHLTEI AMGVRNPVML IRIKPADMPR VVKRKGQESF NFGELFAYTK VCSHLGCPSS QYEQQTYRIL CPCHQSQFDA LHFAKPIFGP AARALAQLPI TIDKDGYLVA NGDFVEPVGP AFWERKS
Validated NT Sequence
atggctcacc accaccacca ccatatgaag aaggccgtgt tgtggacctc ggggtggacg ccccgattcc acggcgagac catctacctg gcgcgggcga ccgggcgtcc cggctcctct ccgttcgtca agatgcggcc cgaggatctc gatgccggtg gcatggagac ggtcttcccc tggcgcgagt ctgacggcga cggcaccacc gtcgagtccg agcaccacct caccgagatc gcgatgggtg tgcgtaaccc ggtcatgttg atccgcatca aaccggccga catgccgcgt gtggtcaagc gaaagggtca ggagagcttc aacttcggtg agctgttcgc ctacaccaag gtctgctcgc acctgggctg cccctcgtcc cagtacgagc agcagaccta ccgcatcctg tgcccgtgcc accagtcgca gttcgacgcg ttgcacttcg caaagcccat tttcggtccg gctgcgcgcg cgttggcgca gctgcccatc accatcgaca aagacggcta cctcgtcgcc aacggcgact tcgtcgaacc cgtcggaccg gcattctggg agcgcaaatc ataagtgagt aagat
Expected Protein Sequence
MAHHHHHHKK AVLWTSGWTP RFHGETIYLA RATGRPGSSP FVKMRPEDLD AGGMETVFPW RESDGDGTTV ESEHHLTEIA MGVRNPVMLI RIKPADMPRV VKRKGQESFN FGELFAYTKV CSHLGCPSSL YEQQTYRILC PCHQSQFDAL HFAKPIFGPA ARALAQLPIT IDKDGYLVAN GDFVEPVGPA FWERKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagaagg ccgtgttgtg gacctcgggg tggacgcccc gattccacgg cgagaccatc tacctggcgc gggcgaccgg gcgtcccggc tcctctccgt tcgtcaagat gcggcccgag gatctcgatg ccggtggcat ggagacggtc ttcccctggc gcgagtctga cggcgacggc accaccgtcg agtccgagca ccacctcacc gagatcgcga tgggtgtgcg taacccggtc atgttgatcc gcatcaaacc ggccgacatg ccgcgtgtgg tcaagcgaaa gggtcaggag agcttcaact tcggtgagct gttcgcctac accaaggtct gctcgcacct gggctgcccc tcgtccctgt acgagcagca gacctaccgc atcctgtgcc cgtgccacca gtcgcagttc gacgcgttgc acttcgcaaa gcccattttc ggtccggctg cgcgcgcgtt ggcgcagctg cccatcacca tcgacaaaga cggctacctc gtcgccaacg gcgacttcgt cgaacccgtc ggaccggcat tctgggagcg caaatcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at