MyfoA.18665.a

ABC transporter

CENTER ID: MyfoA.18665.a
ORGANISM: Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyfoA.18665.a.B2.GE40125 MythA crystallizable fragment 36 406
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1214102.3.peg.1077
UniProt: K0VC89

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPLPVEAARI GATGWQITRT GVRVAANMFG RGSLQQKIIK QVPQTFSDLG PTYVKFGQII ASSPGAFGEP LSREFRGLLD RVPPADTDEV HKLFVEELGE EPTKLFKSFD EKPFASASIA QVHYATLHSG EEVVVKIQRP GIRRRVAADL QILKRGAQLV ELAKLGRRLS AQDVVADFAD NLAEELDFRL EAQSMDAWVS HMHASPLGEN IRVPQVYWDL TSQRVLTMER VTGVRIDDVA AIRKKGFDGT ELVKALLFSV FEGGLKHGLF HGDLHAGNLY VDDDGKIVFF DFGIMGRIDP RTRWLLRELV YALLVKKDHA AAGKIVVLMG AVGTVKPEGE AAKDLEAFAT PLTMKSLGDM SYAEIGRQLS ALADAYDVKL PRELVLIGKQ FLYVERYMKL LAPKWQMMND PQLTGYFANF MVDISREHKE NGDGIEA
NT Sequence
gtgccgctgc ccgtcgaagc cgctcgtatc ggcgccaccg gatggcagat cacccgcacc ggggtccgtg tcgctgccaa catgttcggg cgcggatctc tgcagcagaa gatcatcaag caggtgccgc agacgttctc ggacctgggc cccacctacg tcaagttcgg ccagatcatc gcgtcgagcc cgggggcctt cggtgagccg ctgagccgcg agttccgcgg cctgctcgac cgggtgccac cggccgacac cgatgaggtg cacaagctct tcgtcgagga gctcggcgaa gagcccacca aactgttcaa gagtttcgac gagaagccgt tcgcgtcggc ctcgatcgcc caggtgcact acgccacgct gcacagcggc gaggaggtgg tggtcaagat ccagcgtccc gggatccgcc gccgcgtcgc tgccgatctg cagattctca agcgcggcgc acagctggtg gagctggcca agctgggcag acggctctcc gcgcaggacg tggtggccga cttcgccgac aacctggccg aggagctcga tttccggctg gaagcacagt cgatggacgc ctgggtgtcg cacatgcacg cctctccgct gggcgagaac atccgggtcc ctcaggtgta ctgggacctg accagccagc gggtgctgac gatggagcgg gtcaccgggg tacgcatcga cgacgtggcc gccatccgca agaagggctt cgacggaacc gagctggtca aggccctttt gttcagcgtg ttcgagggcg gtctcaaaca cggcctgttc cacggcgacc tgcacgcggg caacctctac gtcgacgacg acggcaagat cgtgttcttc gatttcggca tcatgggccg gatcgacccc cgcacgcggt ggttgttgcg cgaactggtc tatgccctgc tggtcaagaa ggaccacgcg gcggcgggca agatcgtggt gctgatgggg gccgtaggaa cggtgaagcc cgagggcgag gccgccaagg acctcgaggc gtttgctacc ccgctgacca tgaagtcgct gggcgatatg tcatacgccg aaatcggcag gcagctgtcg gcgctggccg acgcctatga cgtcaagttg ccccgtgaac tggtgttgat cggcaagcag ttcctctacg tcgagcggta catgaagctg ctggccccga agtggcagat gatgaacgat ccccagctca ccgggtactt cgccaacttc atggtcgaca tcagccgcga acacaaggaa aacggcgacg ggatcgaggc ctga
Details for MyfoA.18665.a.B2.GE40125
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 42kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ KIIKQVPQTF SDLGPTYVKF GQIIASSPGA FGEPLSREFR GLLDRVPPAD TDEVHKLFVE ELGEEPTKLF RSFDEKPFAS ASIAQVHYAT LHSGEEVVVK IQRPGIRRRV AADLQILKRG AQLVELAKLG RRLSAQDVVA DFADNLAEEL DFRLEAQSMD AWVSHMHASP LGENIRVPQV YWDLTSQRVL TMERVTGVRI DDVAAIRKKG FDGTELVKAL LFSVFEGGLK HGLFHGDLHA GNLYVDDDGK IVFFDFGIMG RIDPRTRWLL RELVYALLVK KDHAAAGKIV VLMGAVGTVK PEGEAAKDLE AFATPLTMKS LGDMSYAEIG RQLSALADAY DVKLPRELVL IGKQFLYVER YM
Validated NT Sequence
tcatgtaccg ctcgacgtag aggaactgct tgccgatcaa caccagttca cggggcaact tgacgtcata ggcgtcggcc agcgccgaca gctgcctgcc gatttcggcg tatgacatat cgcccagcga cttcatggtc agcggggtag caaacgcctc gaggtccttg gcggcctcgc cctcgggctt caccgttcct acggccccca tcagcaccac gatcttgccc gccgccgcgt ggtccttctt gaccagcagg gcatagacca gttcgcgcaa caaccaccgc gtgcgggggt cgatccggcc catgatgccg aaatcgaaga acacgatctt gccgtcgtcg tcgacgtaga ggttgcccgc gtgcaggtcg ccgtggaaca ggccgtgttt gagaccgccc tcgaacacgc tgaacaaaag ggccttgacc agctcggttc cgtcgaagcc cttcttgcgg atggcggcca cgtcgtcgat gcgtaccccg gtgacccgct ccatcgtcag cacccgctgg ctggtcaggt cccagtacac ctgagggacc cggatgttct cgcccagcgg agaggcgtgc atgtgcgaca cccaggcgtc catcgactgt gcttccagcc ggaaatcgag ctcctcggcc aggttgtcgg cgaagtcggc caccacgtcc tgcgcggaga gccgtctgcc cagcttggcc agctccacca gctgtgcgcc gcgcttgaga atctgcagat cggcagcgac gcggcggcgg atcccgggac gctggatctt gaccaccacc tcctcgccgc tgtgcagcgt ggcgtagtgc acctgggcga tcgaggccga cgcgaacggc ttctcgtcga aactcctgaa cagtttggtg ggctcttcgc cgagctcctc gacgaagagc ttgtgcacct catcggtgtc ggccggtggc acccggtcga gcaggccgcg gaactcgcgg ctcagcggct caccgaaggc ccccgggctc gacgcgatga tctggccgaa cttgacgtag gtggggccca ggtccgagaa cgtctgcggc acctgcttga tgatcttctg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHQK IIKQVPQTFS DLGPTYVKFG QIIASSPGAF GEPLSREFRG LLDRVPPADT DEVHKLFVEE LGEEPTKLFK SFDEKPFASA SIAQVHYATL HSGEEVVVKI QRPGIRRRVA ADLQILKRGA QLVELAKLGR RLSAQDVVAD FADNLAEELD FRLEAQSMDA WVSHMHASPL GENIRVPQVY WDLTSQRVLT MERVTGVRID DVAAIRKKGF DGTELVKALL FSVFEGGLKH GLFHGDLHAG NLYVDDDGKI VFFDFGIMGR IDPRTRWLLR ELVYALLVKK DHAAAGKIVV LMGAVGTVKP EGEAAKDLEA FATPLTMKSL GDMSYAEIGR QLSALADAYD VKLPRELVLI GKQFLYVERY MKLLAPKWQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagaaga tcatcaagca ggtgccgcag acgttctcgg acctgggccc cacctacgtc aagttcggcc agatcatcgc gtcgagcccg ggggccttcg gtgagccgct gagccgcgag ttccgcggcc tgctcgaccg ggtgccaccg gccgacaccg atgaggtgca caagctcttc gtcgaggagc tcggcgaaga gcccaccaaa ctgttcaaga gtttcgacga gaagccgttc gcgtcggcct cgatcgccca ggtgcactac gccacgctgc acagcggcga ggaggtggtg gtcaagatcc agcgtcccgg gatccgccgc cgcgtcgctg ccgatctgca gattctcaag cgcggcgcac agctggtgga gctggccaag ctgggcagac ggctctccgc gcaggacgtg gtggccgact tcgccgacaa cctggccgag gagctcgatt tccggctgga agcacagtcg atggacgcct gggtgtcgca catgcacgcc tctccgctgg gcgagaacat ccgggtccct caggtgtact gggacctgac cagccagcgg gtgctgacga tggagcgggt caccggggta cgcatcgacg acgtggccgc catccgcaag aagggcttcg acggaaccga gctggtcaag gcccttttgt tcagcgtgtt cgagggcggt ctcaaacacg gcctgttcca cggcgacctg cacgcgggca acctctacgt cgacgacgac ggcaagatcg tgttcttcga tttcggcatc atgggccgga tcgacccccg cacgcggtgg ttgttgcgcg aactggtcta tgccctgctg gtcaagaagg accacgcggc ggcgggcaag atcgtggtgc tgatgggggc cgtaggaacg gtgaagcccg agggcgaggc cgccaaggac ctcgaggcgt ttgctacccc gctgaccatg aagtcgctgg gcgatatgtc atacgccgaa atcggcaggc agctgtcggc gctggccgac gcctatgacg tcaagttgcc ccgtgaactg gtgttgatcg gcaagcagtt cctctacgtc gagcggtaca tgaagctgct ggccccgaag tggcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t