SSGCID: Target Page

MyfoA.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS)

CENTER ID:	MyfoA.17943.a
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.17943.a.B1.GE42454	full length		1	957

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.17943.a.B1.PW38508	full length		1	957

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1214102.3.peg.4437
UniProt:	K0URK8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIEPATTTES GQTGPETDTP QHRYTAELAG QIEQSWQQTW AELGTFNVAN PVGSLAPQDG SPLPEDKMFV QDMFPYPSGE GLHVGHPLGY IATDVYARYY RMTGRNVLHA LGFDAFGLPA EQYAVQTGTH PRTRTEANIV NFRRQLGRLG LGHDSRRSFA TTDVDFYKWT QWIFLKIYNA WFDTAAKKAR PISELVAEFE SGARTLDDGR AWAELSAAER ADVVDGYRLV YRADSMVNWC PGLGTVLANE EVTSDGRSDR GNFPVFRKRL RQWMMRITAY SDRLLDDLEV LDWPDKVKSM QRNWIGRSTG ASVEFGTAAG DVEVFTTRPD TLFGATYLVL APEHDLVDAL VADSWPGGTD SRWTFGAATP AEAVAAYRAG IAAKSDLERQ ENKTKTGVFL GAYATNPVNG QQVPVFIADY VLAGYGTGAI MAVPGGDQRD WDFATEFGLP IIEVVAGGDI TEAAYNGDGT MVNSGYLNGL TVAEAKQKVI EHLEADGRGR ARIEYKLRDW LFARQRYWGE PFPIVYDAEG RAHALPESAL PVELPDIPDY SPVSFDPDDA DSEPSPPLGK ATEWVHVELD LGDGLQTYTR DTNVMPQWAG SSWYELRYTD PHNSEELCAK ENEAYWMGPR PAEHGPNDPG GVDLYVGGVE HAVLHLLYSR FWHKVLFDLG IVSSSEPYRR LVNQGYIQAF AYTDSRGSYV PAAEVVEREG KFFWPGPDGE IEVNQEFGKI GKSLKNSVSP DEICDEYGAD TLRVYEMSMG PLEASRPWAT KDVVGAHRFL QRVWRVVVDE STGATSVNEH EALDADTLKI LHRTIAGVTE DYAALRNNTA AAKLIEYTNH LTKEGVSARA AIEPLVLMVA PLAPHLAEEL WKRLGHDTSL AHGPFPQADP QYLVDDTIEF PVQVNGKVRG KITVAADLDK AGLEAAALAD EKVQAFLDGA TPKKVIVVPG RLVNLVV

NT Sequence

gtgatcgaac ccgccaccac taccgagtct gggcagactg gcccggaaac ggacaccccg cagcaccgtt acaccgcgga actggcgggt cagatcgagc agtcctggca gcagacctgg gccgagctcg gcaccttcaa cgttgccaac ccggttggct cgctggcacc acaggacggc tcgccgctgc cggaagacaa gatgttcgtc caggacatgt tcccgtaccc ctcgggtgag ggtctgcacg tcgggcaccc gctgggctac atcgccactg atgtctacgc gcgctactac cggatgacgg gccgcaacgt gctgcacgcg ctgggcttcg atgcgttcgg tctgcctgcc gagcagtacg cggtccagac cgggacgcac ccgcgcaccc gcaccgaggc caacatcgtc aacttccgca ggcagctggg tcggctgggt ctgggccacg attcgcggcg cagcttcgcc accaccgatg tggacttcta caagtggacg cagtggatct tcctgaagat ctacaacgcc tggttcgata ccgcggccaa gaaggcccgg ccgatttccg agctggtggc cgaattcgag tcgggggccc gcactctcga cgacggcagg gcctgggccg agctgtccgc ggccgagcgg gccgacgtgg tggacggcta ccggttggtc taccgcgccg attcgatggt gaactggtgc cccggactgg gcacggtgct ggccaatgaa gaagtcactt ctgacggccg tagcgaccgc ggcaatttcc cggtgttccg gaagcggctg cggcagtgga tgatgcgcat cacggcttac tccgaccggc tgctcgacga tctcgaagtg ctggactggc cggacaaggt caagtccatg cagcgcaact ggatcggccg gtccaccggg gcatcggtgg agttcggtac cgcggccggt gatgtcgagg ttttcaccac ccgcccggat acccttttcg gcgccaccta cctggtgttg gcccccgagc acgatctggt cgacgcgctg gtggccgatt cgtggccagg aggcaccgat tcgcggtgga cgttcggtgc ggcgacgccg gccgaggcgg tcgccgccta ccgcgccggg atcgcagcca agtcggatct ggagcgccag gagaacaaga ccaagaccgg tgtgttcctc ggtgcctacg ccaccaatcc ggtcaacgga cagcaggtgc ctgtcttcat cgccgactac gtgctggccg gatacggcac cggcgccatc atggcggtgc ccggtggcga ccagcgagac tgggacttcg ctaccgagtt cggcctgccc atcatcgagg tggtcgccgg cggtgacatc accgaggcgg cctacaacgg tgacggaacc atggtcaatt ccggctacct gaacgggttg acggtggccg aggccaagca gaaagtcatc gaacacctcg aagccgacgg tcgtggccgg gcgcgcatcg agtacaagct gcgggattgg ctgttcgccc ggcagcggta ctggggtgag ccgttcccga tcgtctacga cgccgagggc cgcgcccatg ctctgccgga atcggctctg ccggtggaac ttccggacat cccggactac tcgccggtgt ccttcgatcc cgacgacgcc gacagtgaac cgtcgccgcc gctgggcaag gccaccgagt gggtgcatgt cgagttggat ctgggtgacg ggttgcagac ctacacccgc gacaccaacg tgatgccgca gtgggcgggc agctcctggt acgagctgcg ttacaccgac ccgcacaatt cagaagagtt gtgcgccaag gagaacgagg cctactggat gggcccgcgc cccgccgagc acggcccgaa cgatccgggt ggcgtcgatc tctacgtcgg tggcgtggag cacgcggtgc tgcacctgct gtactcccgg ttctggcaca aggtgctctt cgacctcggc atcgtcagct cgagcgagcc gtaccgccgt ctggtcaacc agggttacat ccaggccttc gcctacaccg attcgcgcgg aagttatgtg cctgcggccg aagtcgttga gcgcgaaggg aagttcttct ggcccggccc ggacggtgaa atcgaggtga accaggagtt cggcaagatc ggcaagagcc tgaagaactc cgtctcaccg gacgagatct gcgacgagta cggtgcggac accctgcggg tctacgagat gtcgatgggg ccgctggagg cgtcgcggcc gtgggcgacc aaggatgtcg tcggtgcgca ccgcttcctg cagcgggtgt ggcgcgtggt ggtcgatgag tccaccgggg cgacgtcggt caacgagcac gaggccctcg acgccgacac cctcaagatc ctgcaccgca cgatcgccgg cgtgaccgaa gactatgcgg cacttcgcaa caacaccgcg gccgccaagc tgatcgagta caccaaccac ctcaccaagg agggtgtctc ggcccgcgcc gccatcgagc cgctggtcct catggtggcc ccgttggccc cccacctggc cgaggagttg tggaagcggc tgggccacga cacttcgctg gcgcacgggc cgttcccgca ggctgatccg cagtacctgg tggacgacac catcgagttt ccggttcagg tcaacggcaa ggtgcgcggc aagatcaccg tggccgccga tctggacaag gccgggctgg aagccgcggc gctggccgac gagaaggtcc aggcgttcct ggacggtgcc accccgaaga aggtcatcgt ggtgccgggc cgcctggtca acctcgtcgt ctag

Details for MyfoA.17943.a.B1.GE42454

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMI EPATTTESGQ TGPETDTPQH RYTAELAGQI EQSWQQTWAE LGTFNVANPV GSLAPQDGSP LPEDKMFVQD MFPYPSGEGL HVGHPLGYIA TDVYARYYRM TGRNVLHALG FDAFGLPAEQ YAVQTGTHPR TRTEANIVNF RRQLGRLGLG HDSRRSFATT DVDFYKWTQW IFLKIYNAWF DTAAKKARPI SELVAEFESG ARTLDDGRAW AELSAAERAD VVDGYRLVYR ADSMVNWCPG LGTVLANEEV TSDGRSDRGN FPVFRKRLRQ WMMRITAYSD RLLDDLEVLD WPDKVKSMQR NWIGRSTGAS VEFGTAAGDV EVFTTRPDTL FGATYLVLAP EHDLVDALVA DSWPGGTDSR WTFGAATPAE AVAAYRAGIA AKSDLERQEN KTKTGVFLGA YATNPVNGQQ VPVFIADYVL AGYGTGAIMA VPGGDQRDWD FATEFGLPII EVVAGGDITE AAYNGDGTMV NSGYLNGLTV AEAKQKVIEH LEADGRGRAR IEYKLRDWLF ARQRYWGEPF PIVYDAEGRA HALPESALPV ELPDIPDYSP VSFDPDDADS EPSPPLGKAT EWVHVELDLG DGLQTYTRDT NVMPQWAGSS WYELRYTDPH NSEELCAKEN EAYWMGPRPA EHGPNDPGGV DLYVGGVEHA VLHLLYSRFW HKVLFDLGIV SSSEPYRRLV NQGYIQAFAY TDSRGSYVPA AEVVEREGKF FWPGPDGEIE VNQEFGKIGK SLKNSVSPDE ICDEYGADTL RVYEMSMGPL EASRPWATKD VVGAHRFLQR VWRVVVDEST GATSVNEHEA LDADTLKILH RTIAGVTEDY AALRNNTAAA KLIEYTNHLT KEGVSARAAI EPLVLMVAPL APHLAEELWK RLGHDTSLAH GPFPQADPQY LVDDTIEFPV QVNGKVRGKI TVAADLDKAG LEAAALADEK VQAFLDGATP KKVIVVPGRL VNLVV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgatcg aacccgccac cactaccgag tctgggcaga ctggcccgga aacggacacc ccgcagcacc gttacaccgc ggaactggcg ggtcagatcg agcagtcctg gcagcagacc tgggccgagc tcggcacctt caacgttgcc aacccggttg gctcgctggc accacaggac ggctcgccgc tgccggaaga caagatgttc gtccaggaca tgttcccgta cccctcgggt gagggtctgc acgtcgggca cccgctgggc tacatcgcca ctgatgtcta cgcgcgctac taccggatga cgggccgcaa cgtgctgcac gcgctgggct tcgatgcgtt cggtctgcct gccgagcagt acgcggtcca gaccgggacg cacccgcgca cccgcaccga ggccaacatc gtcaacttcc gcaggcagct gggtcggctg ggtctgggcc acgattcgcg gcgcagcttc gccaccaccg atgtggactt ctacaagtgg acgcagtgga tcttcctgaa gatctacaac gcctggttcg ataccgcggc caagaaggcc cggccgattt ccgagctggt ggccgaattc gagtcggggg cccgcactct cgacgacggc agggcctggg ccgagctgtc cgcggccgag cgggccgacg tggtggacgg ctaccggttg gtctaccgcg ccgattcgat ggtgaactgg tgccccggac tgggcacggt gctggccaat gaagaagtca cttctgacgg ccgtagcgac cgcggcaatt tcccggtgtt ccggaagcgg ctgcggcagt ggatgatgcg catcacggct tactccgacc ggctgctcga cgatctcgaa gtgctggact ggccggacaa ggtcaagtcc atgcagcgca actggatcgg ccggtccacc ggggcatcgg tggagttcgg taccgcggcc ggtgatgtcg aggttttcac cacccgcccg gatacccttt tcggcgccac ctacctggtg ttggcccccg agcacgatct ggtcgacgcg ctggtggccg attcgtggcc aggaggcacc gattcgcggt ggacgttcgg tgcggcgacg ccggccgagg cggtcgccgc ctaccgcgcc gggatcgcag ccaagtcgga tctggagcgc caggagaaca agaccaagac cggtgtgttc ctcggtgcct acgccaccaa tccggtcaac ggacagcagg tgcctgtctt catcgccgac tacgtgctgg ccggatacgg caccggcgcc atcatggcgg tgcccggtgg cgaccagcga gactgggact tcgctaccga gttcggcctg cccatcatcg aggtggtcgc cggcggtgac atcaccgagg cggcctacaa cggtgacgga accatggtca attccggcta cctgaacggg ttgacggtgg ccgaggccaa gcagaaagtc atcgaacacc tcgaagccga cggtcgtggc cgggcgcgca tcgagtacaa gctgcgggat tggctgttcg cccggcagcg gtactggggt gagccgttcc cgatcgtcta cgacgccgag ggccgcgccc atgctctgcc ggaatcggct ctgccggtgg aacttccgga catcccggac tactcgccgg tgtccttcga tcccgacgac gccgacagtg aaccgtcgcc gccgctgggc aaggccaccg agtgggtgca tgtcgagttg gatctgggtg acgggttgca gacctacacc cgcgacacca acgtgatgcc gcagtgggcg ggcagctcct ggtacgagct gcgttacacc gacccgcaca attcagaaga gttgtgcgcc aaggagaacg aggcctactg gatgggcccg cgccccgccg agcacggccc gaacgatccg ggtggcgtcg atctctacgt cggtggcgtg gagcacgcgg tgctgcacct gctgtactcc cggttctggc acaaggtgct cttcgacctc ggcatcgtca gctcgagcga gccgtaccgc cgtctggtca accagggtta catccaggcc ttcgcctaca ccgattcgcg cggaagttat gtgcctgcgg ccgaagtcgt tgagcgcgaa gggaagttct tctggcccgg cccggacggt gaaatcgagg tgaaccagga gttcggcaag atcggcaaga gcctgaagaa ctccgtctca ccggacgaga tctgcgacga gtacggtgcg gacaccctgc gggtctacga gatgtcgatg gggccgctgg aggcgtcgcg gccgtgggcg accaaggatg tcgtcggtgc gcaccgcttc ctgcagcggg tgtggcgcgt ggtggtcgat gagtccaccg gggcgacgtc ggtcaacgag cacgaggccc tcgacgccga caccctcaag atcctgcacc gcacgatcgc cggcgtgacc gaagactatg cggcacttcg caacaacacc gcggccgcca agctgatcga gtacaccaac cacctcacca aggagggtgt ctcggcccgc gccgccatcg agccgctggt cctcatggtg gccccgttgg ccccccacct ggccgaggag ttgtggaagc ggctgggcca cgacacttcg ctggcgcacg ggccgttccc gcaggctgat ccgcagtacc tggtggacga caccatcgag tttccggttc aggtcaacgg caaggtgcgc ggcaagatca ccgtggccgc cgatctggac aaggccgggc tggaagccgc ggcgctggcc gacgagaagg tccaggcgtt cctggacggt gccaccccga agaaggtcat cgtggtgccg ggccgcctgg tcaacctcgt cgtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for MyfoA.17943.a.B1.PW38508

PURIFICATION DATe:	7/9/2018
CONCENTRATION:	17.8mg/ml
OBSERVED MW:	107kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGATCG AACCCGCCAC CACTACCGAG TCTGGGCAGA CTGGCCCGGA AACGGACACC CCGCAGCACC GTTACACCGC GGAACTGGCG GGTCAGATCG AGCAGTCCTG GCAGCAGACC TGGGCCGAGC TCGGCACCTT CAACGTTGCC AACCCGGTTG GCTCGCTGGC ACCACAGGAC GGCTCGCCGC TGCCGGAAGA CAAGATGTTC GTCCAGGACA TGTTCCCGTA CCCCTCGGGT GAGGGTCTGC ACGTCGGGCA CCCGCTGGGC TACATCGCCA CTGATGTCTA CGCGCGCTAC TACCGGATGA CGGGCCGCAA CGTGCTGCAC GCGCTGGGCT TCGATGCGTT CGGTCTGCCT GCCGAGCAGT ACGCGGTCCA GACCGGGACG CACCCGCGCA CCCGCACCGA GGCCAACATC GTCAACTTCC GCAGGCAGCT GGGTCGGCTG GGTCTGGGCC ACGATTCGCG GCGCAGCTTC GCCACCACCG ATGTGGACTT CTACAAGTGG ACGCAGTGGA TCTTCCTGAA GATCTACAAC GCCTGGTTCG ATACCGCGGC CAAGAAGGCC CGGCCGATTT CCGAGCTGGT GGCCGAATTC GAGTCGGGGG CCCGCACTCT CGACGACGGC AGGGCCTGGG CCGAGCTGTC CGCGGCCGAG CGGGCCGACG TGGTGGACGG CTACCGGTTG GTCTACCGCG CCGATTCGAT GGTGAACTGG TGCCCCGGAC TGGGCACGGT GCTGGCCAAT GAAGAAGTCA CTTCTGACGG CCGTAGCGAC CGCGGCAATT TCCCGGTGTT CCGGAAGCGG CTGCGGCAGT GGATGATGCG CATCACGGCT TACTCCGACC GGCTGCTCGA CGATCTCGAA GTGCTGGACT GGCCGGACAA GGTCAAGTCC ATGCAGCGCA ACTGGATCGG CCGGTCCACC GGGGCATCGG TGGAGTTCGG TACCGCGGCC GGTGATGTCG AGGTTTTCAC CACCCGCCCG GATACCCTTT TCGGCGCCAC CTACCTGGTG TTGGCCCCCG AGCACGATCT GGTCGACGCG CTGGTGGCCG ATTCGTGGCC AGGAGGCACC GATTCGCGGT GGACGTTCGG TGCGGCGACG CCGGCCGAGG CGGTCGCCGC CTACCGCGCC GGGATCGCAG CCAAGTCGGA TCTGGAGCGC CAGGAGAACA AGACCAAGAC CGGTGTGTTC CTCGGTGCCT ACGCCACCAA TCCGGTCAAC GGACAGCAGG TGCCTGTCTT CATCGCCGAC TACGTGCTGG CCGGATACGG CACCGGCGCC ATCATGGCGG TGCCCGGTGG CGACCAGCGA GACTGGGACT TCGCTACCGA GTTCGGCCTG CCCATCATCG AGGTGGTCGC CGGCGGTGAC ATCACCGAGG CGGCCTACAA CGGTGACGGA ACCATGGTCA ATTCCGGCTA CCTGAACGGG TTGACGGTGG CCGAGGCCAA GCAGAAAGTC ATCGAACACC TCGAAGCCGA CGGTCGTGGC CGGGCGCGCA TCGAGTACAA GCTGCGGGAT TGGCTGTTCG CCCGGCAGCG GTACTGGGGT GAGCCGTTCC CGATCGTCTA CGACGCCGAG GGCCGCGCCC ATGCTCTGCC GGAATCGGCT CTGCCGGTGG AACTTCCGGA CATCCCGGAC TACTCGCCGG TGTCCTTCGA TCCCGACGAC GCCGACAGTG AACCGTCGCC GCCGCTGGGC AAGGCCACCG AGTGGGTGCA TGTCGAGTTG GATCTGGGTG ACGGGTTGCA GACCTACACC CGCGACACCA ACGTGATGCC GCAGTGGGCG GGCAGCTCCT GGTACGAGCT GCGTTACACC GACCCGCACA ATTCAGAAGA GTTGTGCGCC AAGGAGAACG AGGCCTACTG GATGGGCCCG CGCCCCGCCG AGCACGGCCC GAACGATCCG GGTGGCGTCG ATCTCTACGT CGGTGGCGTG GAGCACGCGG TGCTGCACCT GCTGTACTCC CGGTTCTGGC ACAAGGTGCT CTTCGACCTC GGCATCGTCA GCTCGAGCGA GCCGTACCGC CGTCTGGTCA ACCAGGGTTA CATCCAGGCC TTCGCCTACA CCGATTCGCG CGGAAGTTAT GTGCCTGCGG CCGAAGTCGT TGAGCGCGAA GGGAAGTTCT TCTGGCCCGG CCCGGACGGT GAAATCGAGG TGAACCAGGA GTTCGGCAAG ATCGGCAAGA GCCTGAAGAA CTCCGTCTCA CCGGACGAGA TCTGCGACGA GTACGGTGCG GACACCCTGC GGGTCTACGA GATGTCGATG GGGCCGCTGG AGGCGTCGCG GCCGTGGGCG ACCAAGGATG TCGTCGGTGC GCACCGCTTC CTGCAGCGGG TGTGGCGCGT GGTGGTCGAT GAGTCCACCG GGGCGACGTC GGTCAACGAG CACGAGGCCC TCGACGCCGA CACCCTCAAG ATCCTGCACC GCACGATCGC CGGCGTGACC GAAGACTATG CGGCACTTCG CAACAACACC GCGGCCGCCA AGCTGATCGA GTACACCAAC CACCTCACCA AGGAGGGTGT CTCGGCCCGC GCCGCCATCG AGCCGCTGGT CCTCATGGTG GCCCCGTTGG CCCCCCACCT GGCCGAGGAG TTGTGGAAGC GGCTGGGCCA CGACACTTCG CTGGCGCACG GGCCGTTCCC GCAGGCTGAT CCGCAGTACC TGGTGGACGA CACCATCGAG TTTCCGGTTC AGGTCAACGG CAAGGTGCGC GGCAAGATCA CCGTGGCCGC CGATCTGGAC AAGGCCGGGC TGGAAGCCGC GGCGCTGGCC GACGAGAAGG TCCAGGCGTT CCTGGACGGT GCCACCCCGA AGAAGGTCAT CGTGGTGCCG GGCCGCCTGG TCAACCTCGT CGTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT