SSGCID: Target Page

MyfoA.17861.m

DNA-directed RNA polymerase (Rv0667 ortholog)

CENTER ID:	MyfoA.17861.m
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.17861.m.B1.GE38102	full length		1	223

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	B3VMJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

ELTPEERLLR AIFGEKAREV RDTSLKVPHG ESGKVIGIRV FSREDDDELP AGVNELVRVY VAQKRKISDG DKLAGRHGNK GVIGKILPVE DMPFLPDGTP VDIILNTHGV PRRMNIGQIL ETHLGWVAKA GWNIDVAAGT PEWASKLPEQ LYSAPVDSIV STPVFDGARE EELAGLLGST LPNRDGDVMV NADGKATLFD GRSGEPFPYP VTVGYMYILK LHH

NT Sequence

GAGCTGACCC CGGAGGAGCG CCTGCTGCGT GCCATCTTCG GTGAGAAGGC CCGCGAGGTT CGCGACACCT CGCTGAAGGT GCCCCACGGC GAGTCCGGCA AGGTCATCGG CATCCGCGTG TTCTCTCGCG AGGACGACGA CGAGCTGCCC GCCGGTGTCA ACGAGCTGGT CCGCGTCTAC GTGGCCCAGA AGCGCAAGAT CTCCGACGGC GACAAGCTCG CCGGACGCCA CGGCAACAAG GGCGTCATCG GCAAGATCCT GCCGGTCGAG GACATGCCGT TCCTGCCCGA TGGCACCCCG GTGGACATCA TCCTGAACAC CCACGGTGTG CCGCGTCGTA TGAACATCGG CCAGATCCTG GAAACCCACC TCGGGTGGGT GGCCAAGGCC GGCTGGAACA TCGATGTGGC TGCGGGCACT CCGGAGTGGG CGTCGAAGCT GCCCGAGCAG CTGTACTCGG CCCCGGTCGA CAGCATCGTG TCCACCCCGG TGTTCGACGG TGCCCGCGAA GAGGAGCTGG CCGGTCTGCT CGGCTCGACG CTGCCCAACC GTGACGGCGA CGTCATGGTG AATGCCGACG GCAAGGCGAC GTTGTTCGAC GGCCGCAGTG GCGAACCGTT CCCGTACCCG GTGACGGTCG GCTACATGTA CATCCTCAAG CTGCACCAC

Details for MyfoA.17861.m.B1.GE38102

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME LTPEERLLRA IFGEKAREVR DTSLKVPHGE SGKVIGIRVF SREDDDELPA GVNELVRVYV AQKRKISDGD KLAGRHGNKG VIGKILPVED MPFLPDGTPV DIILNTHGVP RRMNIGQILE THLGWVAKAG WNIDVAAGTP EWASKLPEQL YSAPVDSIVS TPVFDGAREE ELAGLLGSTL PNRDGDVMVN ADGKATLFDG RSGEPFPYPV TVGYMYILKL HH

Validated NT Sequence

atcttactca cttagtggtg cagcttgagg atgtacatgt agccgaccgt caccgggtac gggaacggtt cgccactgcg gccgtcgaac aacgtcgcct tgccgtcggc attcaccatg acgtcgccgt cacggttggg cagcgtcgag ccgagcagac cggccagctc ctcttcgcgg gcaccgtcga acaccggggt ggacacgatg ctgtcgaccg gggccgagta cagctgctcg ggcagcttcg acgcccattc cggagtgccc gcagccacat cgatgttcca gccggccttg gccacccacc cgaggtgggt ttccaggatc tggccgatgt tcatacgacg cggcacaccg tgggtgttca ggatgatgtc caccggggtg ccatcgggca ggaacggcat gtcctcgacc ggcaggatct tgccgatgac gcccttgttg ccgtggcgtc cggcgagctt gtcgccgtcg gagatcttgc gcttctgggc cacgtagacg cggaccagct cgttgacacc ggcgggcagc tcgtcgtcgt cctcgcgaga gaacacgcgg atgccgatga ccttgccgga ctcgccgtgg ggcaccttca gcgaggtgtc acgaacctcg cgggccttct caccgaagat ggcacgcagc aggcgctcct ccggggtcag ctccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHEL TPEERLLRAI FGEKAREVRD TSLKVPHGES GKVIGIRVFS REDDDELPAG VNELVRVYVA QKRKISDGDK LAGRHGNKGV IGKILPVEDM PFLPDGTPVD IILNTHGVPR RMNIGQILET HLGWVAKAGW NIDVAAGTPE WASKLPEQLY SAPVDSIVST PVFDGAREEE LAGLLGSTLP NRDGDVMVNA DGKATLFDGR SGEPFPYPVT VGYMYILKLH H

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagctga ccccggagga gcgcctgctg cgtgccatct tcggtgagaa ggcccgcgag gttcgcgaca cctcgctgaa ggtgccccac ggcgagtccg gcaaggtcat cggcatccgc gtgttctctc gcgaggacga cgacgagctg cccgccggtg tcaacgagct ggtccgcgtc tacgtggccc agaagcgcaa gatctccgac ggcgacaagc tcgccggacg ccacggcaac aagggcgtca tcggcaagat cctgccggtc gaggacatgc cgttcctgcc cgatggcacc ccggtggaca tcatcctgaa cacccacggt gtgccgcgtc gtatgaacat cggccagatc ctggaaaccc acctcgggtg ggtggccaag gccggctgga acatcgatgt ggctgcgggc actccggagt gggcgtcgaa gctgcccgag cagctgtact cggccccggt cgacagcatc gtgtccaccc cggtgttcga cggtgcccgc gaagaggagc tggccggtct gctcggctcg acgctgccca accgtgacgg cgacgtcatg gtgaatgccg acggcaaggc gacgttgttc gacggccgca gtggcgaacc gttcccgtac ccggtgacgg tcggctacat gtacatcctc aagctgcacc actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat