SSGCID: Target Page

MyfoA.17391.b

RNA polymerase sigma factor SigF (Fragment) (Rv3286c ortholog)

CENTER ID:	MyfoA.17391.b
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.17391.b.B1.GE40124	full length		1	152

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1214102.3.peg.1556
UniProt:	K0VB57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

NSWSVKVPRR LKELHLRLGA ATAELSQRMG RAPTASELAE ELGMDREEVI EGLVAGSSYN TLSIDSGGGG DEDAPAIVDT LGDVDTGLDQ IDNRETLRPL LAQLPERERT VLLLRFFESM TQTQIAERVG VSQMHVSRLL AKSLARLRDQ LQ

NT Sequence

AACAGCTGGT CGGTGAAGGT GCCGCGGCGG CTCAAGGAGC TGCACCTGCG GCTCGGCGCC GCGACCGCGG AACTGTCCCA GCGGATGGGG CGCGCTCCGA CGGCTTCTGA GCTGGCCGAG GAACTCGGCA TGGACCGTGA AGAAGTGATC GAAGGCCTGG TGGCGGGCAG CTCGTACAAC ACGCTGTCGA TCGACAGCGG TGGCGGAGGG GACGAGGACG CGCCGGCGAT AGTCGACACC CTCGGCGACG TGGACACGGG CCTGGATCAG ATCGACAATC GGGAAACGTT GCGGCCGTTG CTTGCTCAAC TACCTGAGCG GGAACGGACA GTGCTGTTGC TGCGGTTCTT CGAATCCATG ACGCAGACGC AGATCGCCGA ACGTGTCGGT GTCTCGCAGA TGCACGTTTC GCGACTGTTG GCGAAATCGC TAGCGCGACT TCGTGACCAG CTGCAGTAG

Details for MyfoA.17391.b.B1.GE40124

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/13/2016
EXPECTED MW:	18kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN SWSVKVPRRL KELHLRLGAA TAELSQRMGR APTASELAEE LGMDREEVIE GLVAGSSYNT LSIDSGGGGD EDAPAIVDTL GDVDTGLDQI DNRETLRPLL AQLPERERTV LLLRFFESMT QTQIAERVGV SQMHVSRLLA KSLARLRDQL Q

Validated NT Sequence

atcttactca cttactgcag ctggtcacga agtcgcgcta gcgatttcgc caacagtcgc gaaacgtgca tctgcgagac accgacacgt tcggcgatct gcgtctgcgt catggattcg aagaaccgca gcaacagcac tgtccgttcc cgctcaggta gttgagcaag caacggccgc aacgtttccc gattgtcgat ctgatccagg cccgtgtcca cgtcgccgag ggtgtcgact atcgccggcg cgtcctcgtc ccctccgcca ccgctgtcga tcgacagcgt gttgtacgag ctgcccgcca ccaggccttc gatcacttct tcacggtcca tgccgagttc ctcggccagc tcagaagccg tcggagcgcg ccccatccgc tgggacagtt ccgcggtcgc ggcgccgagc cgcaggtgca gctccttgag ccgccgcggc accttcaccg accagctgtt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHNS WSVKVPRRLK ELHLRLGAAT AELSQRMGRA PTASELAEEL GMDREEVIEG LVAGSSYNTL SIDSGGGGDE DAPAIVDTLG DVDTGLDQID NRETLRPLLA QLPERERTVL LLRFFESMTQ TQIAERVGVS QMHVSRLLAK SLARLRDQLQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataacagct ggtcggtgaa ggtgccgcgg cggctcaagg agctgcacct gcggctcggc gccgcgaccg cggaactgtc ccagcggatg gggcgcgctc cgacggcttc tgagctggcc gaggaactcg gcatggaccg tgaagaagtg atcgaaggcc tggtggcggg cagctcgtac aacacgctgt cgatcgacag cggtggcgga ggggacgagg acgcgccggc gatagtcgac accctcggcg acgtggacac gggcctggat cagatcgaca atcgggaaac gttgcggccg ttgcttgctc aactacctga gcgggaacgg acagtgctgt tgctgcggtt cttcgaatcc atgacgcaga cgcagatcgc cgaacgtgtc ggtgtctcgc agatgcacgt ttcgcgactg ttggcgaaat cgctagcgcg acttcgtgac cagctgcagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat