MyfoA.17190.a

ATPase AAA (Fragment) (Rv3610c ortholog)

CENTER ID: MyfoA.17190.a
ORGANISM: Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyfoA.17190.a.B1.GE37820 full length 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1214102.3.peg.5643
UniProt: K0UM05

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
TEPMLWQEDL TGALTVIRPL SRSAAEEVSV GSVTLDDVGD MVETKQALTE AVLWPLQHPD TFQRLGVEPP RGVLLYGPPG CGKTFLVRAL ASSGKLSVHA VKGAELMDKW VGSSEKAVRE LFARARDSAP SLVFLDEIDA LAPRRGQSFD SGVTDRVVAS LLTELDGIEP MNDVVVLGAT NRPDLIDPAL LRPGRLEKLV FVEPPDADAR REILRTSGKS IPLAADVDLD ALASELDGYS AADCVALLRE AALTAMRRSI DAADVTAADV AKARETVRPS LDAAQVQSLR EFAAGR
NT Sequence
ACCGAGCCGA TGCTGTGGCA GGAAGACCTG ACCGGGGCAC TGACCGTGAT CCGGCCCTTG TCGAGGTCGG CGGCCGAGGA GGTGTCGGTC GGCTCGGTAA CGCTCGACGA CGTGGGCGAC ATGGTCGAGA CCAAGCAGGC GCTCACCGAG GCGGTGCTGT GGCCGCTGCA GCACCCGGAC ACCTTCCAGC GCCTCGGCGT GGAACCGCCC CGCGGTGTAC TGCTCTACGG ACCGCCCGGC TGCGGAAAGA CCTTCCTGGT GCGGGCCTTG GCCAGCTCCG GCAAGTTGTC CGTGCACGCG GTCAAAGGTG CTGAGCTGAT GGACAAGTGG GTCGGCTCGT CGGAGAAGGC AGTGCGCGAA CTGTTCGCCC GCGCACGTGA CTCGGCACCG TCACTGGTGT TCCTCGACGA GATCGACGCG CTGGCTCCGC GGCGCGGACA GAGCTTCGAT TCCGGGGTGA CCGACCGGGT GGTGGCCTCG CTGCTGACCG AGCTCGACGG CATCGAACCG ATGAACGACG TCGTCGTGCT GGGTGCGACG AACCGCCCCG ATCTCATCGA CCCCGCGCTG CTCCGGCCCG GCCGGTTGGA GAAGCTGGTG TTCGTGGAGC CGCCCGATGC CGATGCTCGA CGCGAAATCC TGCGCACCTC AGGCAAATCC ATTCCGCTTG CCGCCGACGT CGATCTCGAC GCCCTGGCTT CCGAGCTGGA CGGCTACAGC GCGGCCGACT GTGTCGCACT GCTGCGGGAG GCCGCGTTGA CGGCCATGCG CCGGTCGATC GACGCTGCGG ACGTCACGGC CGCCGACGTC GCGAAGGCTC GCGAGACCGT ACGGCCGTCA CTTGATGCCG CGCAGGTGCA GTCACTGCGG GAGTTCGCCG CCGGGCGCTG A
Details for MyfoA.17190.a.B1.GE37820
HARVESTED ON: 3/6/2014
SEQUENCED ON: 3/13/2014
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT EPMLWQEDLT GALTVIRPLS RSAAEEVSVG SVTLDDVGDM VETKQALTEA VLWPLQHPDT FQRLGVEPPR GVLLYGPPGC GKTFLVRALA SSGKLSVHAV KGAELMDKWV GSSEKAVREL FARARDSAPS LVFLDEIDAL APRRGQSFDS GVTDRVVASL LTELDGIEPM NDVVVLGATN RPDLIDPALL RPGRLEKLVF VEPPDADARR EILRTSGKSI PLAADVDLDA LASELDGYSA ADCVALLREA ALTAMRRSID AADVTAADVA KARETVRPSL DAAQVQSLRE FAAGR
Validated NT Sequence
atcttactca cttagcgccc ggcggcgaac tcccgcagtg actgcacctg cgcggcatca agtgacggcc gtacggtctc gcgagccttc gcgacgtcgg cggccgtgac gtccgcagcg tcgatcgacc ggcgcatggc cgtcaacgcg gcctcccgca gcagtgcgac acagtcggcc gcgctgtagc cgtccagctc ggaagccagg gcgtcgagat cgacgtcggc ggcaagcgga atggatttgc ctgaggtgcg caggatttcg cgtcgagcat cggcatcggg cggctccacg aacaccagct tctccaaccg gccgggccgg agcagcgcgg ggtcgatgag atcggggcgg ttcgtcgcac ccagcacgac gacgtcgttc atcggttcga tgccgtcgag ctcggtcagc agcgaggcca ccacccggtc ggtcaccccg gaatcgaagc tctgtccgcg ccgcggagcc agcgcgtcga tctcgtcgag gaacaccagt gacggtgccg agtcacgtgc gcgggcgaac agttcgcgca ctgccttctc cgacgagccg acccacttgt ccatcagctc agcacctttg accgcgtgca cggacaactt gccggagctg gccaaggccc gcaccaggaa ggtctttccg cagccgggcg gtccgtagag cagtacaccg cggggcggtt ccacgccgag gcgctggaag gtgtccgggt gctgcagcgg ccacagcacc gcctcggtga gcgcctgctt ggtctcgacc atgtcgccca cgtcgtcgag cgttaccgag ccgaccgaca cctcctcggc cgccgacctc gacaagggcc ggatcacggt cagtgccccg gtcaggtctt cctgccacag catcggctcg gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHTE PMLWQEDLTG ALTVIRPLSR SAAEEVSVGS VTLDDVGDMV ETKQALTEAV LWPLQHPDTF QRLGVEPPRG VLLYGPPGCG KTFLVRALAS SGKLSVHAVK GAELMDKWVG SSEKAVRELF ARARDSAPSL VFLDEIDALA PRRGQSFDSG VTDRVVASLL TELDGIEPMN DVVVLGATNR PDLIDPALLR PGRLEKLVFV EPPDADARRE ILRTSGKSIP LAADVDLDAL ASELDGYSAA DCVALLREAA LTAMRRSIDA ADVTAADVAK ARETVRPSLD AAQVQSLREF AAGR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataccgagc cgatgctgtg gcaggaagac ctgaccgggg cactgaccgt gatccggccc ttgtcgaggt cggcggccga ggaggtgtcg gtcggctcgg taacgctcga cgacgtgggc gacatggtcg agaccaagca ggcgctcacc gaggcggtgc tgtggccgct gcagcacccg gacaccttcc agcgcctcgg cgtggaaccg ccccgcggtg tactgctcta cggaccgccc ggctgcggaa agaccttcct ggtgcgggcc ttggccagct ccggcaagtt gtccgtgcac gcggtcaaag gtgctgagct gatggacaag tgggtcggct cgtcggagaa ggcagtgcgc gaactgttcg cccgcgcacg tgactcggca ccgtcactgg tgttcctcga cgagatcgac gcgctggctc cgcggcgcgg acagagcttc gattccgggg tgaccgaccg ggtggtggcc tcgctgctga ccgagctcga cggcatcgaa ccgatgaacg acgtcgtcgt gctgggtgcg acgaaccgcc ccgatctcat cgaccccgcg ctgctccggc ccggccggtt ggagaagctg gtgttcgtgg agccgcccga tgccgatgct cgacgcgaaa tcctgcgcac ctcaggcaaa tccattccgc ttgccgccga cgtcgatctc gacgccctgg cttccgagct ggacggctac agcgcggccg actgtgtcgc actgctgcgg gaggccgcgt tgacggccat gcgccggtcg atcgacgctg cggacgtcac ggccgccgac gtcgcgaagg ctcgcgagac cgtacggccg tcacttgatg ccgcgcaggt gcagtcactg cgggagttcg ccgccgggcg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat