MyfoA.00629.d

Peptide arylation enzyme

CENTER ID: MyfoA.00629.d
ORGANISM: Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyfoA.00629.d.B2.GE40120 ordered domain 15 550
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1214102.3.peg.94
RefSeq: WP_003879648.1
UniProt: K0VF23

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTGFDREQD ELANGFTRFP EARAEVYRQA GYWQGKALDS ILREAAQRWP DKAAVVDASG TYSFAELDAL ADRIGAALAA QGIHQGDRVL VQLPNSREFA VATFGLLRAG VVPVLCLPGH RSAELGHFAE VSGAVGLVIP DVIAGFDYRD LAAELVSEHP RLRHILVDGE PGSFQSWSAL KDFENPGPPD ITVDTSGPAV LLVSGGTTGL PKLIPRTHDD YLYNAAACAQ ACEMVHDDVY LAVLPAGHNF PFACPGLLGS MTVGATTVYT DDPSPESAFA LINTHGVTVT GLVNALAKLW AQACEWEPVL PTSLRVVQVG GSRMSPEEAR FIREGLSPGL QQIFGMAEGM LNFTRPGDPV DVVENTQGRP MSPHDEMRVV DEDGHDVAPG QEGELLVRGP YTLNGYYRAE EANARSFSPD GFYRTGDRVR IFTDGPRAGY VEVTGRIKDV IHRGGETVSA TDLEDHLLSH PAVHTAAAVA LPDDYLGEKI CAAVVFNGAP ISLVELNKFL DQRGASAHAR PDVLVPMPAM PTTAVGKIDK KKLIAAITER
NT Sequence
atgaccaccg gttttgaccg cgaacaggac gaactggcaa acggattcac ccggtttccc gaagcccgtg ccgaggtata ccggcaggcc ggctactggc aaggcaaagc actggacagc atccttcgcg aggctgcgca acgctggccc gacaaggcgg ccgtcgtcga cgcctcaggc acctacagtt tcgccgaatt ggatgcgctc gccgaccgca tcggtgccgc actcgccgcc cagggaatcc atcagggcga ccgcgtcctg gtgcaactgc cgaactcgcg cgaattcgcg gtggccactt tcggtttgct tcgcgccggc gtggtgcccg tgctgtgcct acccgggcac cggtctgccg agttgggcca cttcgccgag gtcagcggcg ccgtcggact cgtgatcccc gacgtcatcg ccggattcga ctaccgcgac ctggccgccg aactcgtgtc cgagcatccg cgactgcgcc acatcctcgt cgacggcgaa cccggcagct ttcaatcctg gtcagcgctc aaggatttcg agaatccggg gcctcccgac atcaccgtcg acaccagcgg tcccgcagtc ctgctggtgt ccggcggaac caccgggctg ccgaagctca tcccccgcac tcacgacgat tacctctaca acgccgcggc ctgcgcccag gcgtgcgaga tggtgcatga cgacgtctat ctggcggtgt tgccggccgg ccacaacttc ccgttcgcgt gcccgggcct gctcggctcg atgacggtcg gagccacaac ggtctacacc gacgatccca gtccagaatc agctttcgcc ttgatcaaca cccacggcgt cacggtgacc ggactcgtca acgccctggc caaactgtgg gcccaggcct gcgaatggga accggtgcta cccacgtccc tgcgtgtggt gcaggtgggc ggatcgcgga tgtcacccga ggaggcccgc ttcatccggg aaggcctcag cccgggactg cagcagatct tcggaatggc cgaaggaatg ctgaatttca cgcgccccgg tgatccggtg gacgtggtgg agaacaccca gggccgaccg atgtcgccgc atgacgagat gcgcgtcgtc gacgaggacg gccacgacgt cgccccgggc caggagggtg aactactggt ccgcggaccg tacacgctca acggctatta ccgcgccgag gaggccaatg cccgctcctt cagccctgac ggcttctacc gcaccggcga tcgggtacgc atcttcaccg acggaccgcg ggccggctac gtcgaggtca cgggccggat caaggacgtc atccaccgtg gcggtgagac cgtgtcggcc acagacctcg aggaccatct gctgagccac cctgcggtgc acacagccgc agcggtggca ctgcctgatg actacctggg cgagaagatc tgcgccgcag tggttttcaa cggagcgccg atcagcctgg tggaactgaa caagttcctc gaccagcggg gcgcatcggc gcatgcgcgc cccgacgtac tggttccgat gcccgccatg cccaccacgg cggtcggcaa gatcgacaag aagaagctga tcgccgcaat caccgagcga tga
Details for MyfoA.00629.d.B2.GE40120
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 58kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 84
PERCENT COVERAGE: 64
Validated AA Sequence
MAHHHHHHMG FTRFPEARAE VYRQAGYWQG KALDSILREA AQRWPDKAAV VDASGTYSFA ELDALADRIG AALAAQGIHQ GDRVLVQLPN SREFAVATFG LLRAGVVPVL CLPGHRSAEL GHFAEVSGAV GLVIPDVIAG FDYRDLAAEL VSEHPRLRHI LVDGEPGSFQ SWSALKDFEN PGPPDITVDT SGPAVLLVSG GTTGLPKLIP RTHDDHLYNA AACAQACEMV HDDVYLAVLP AGHNFPFACP GLLGSMTVGA TTVYTDDXXS RISFRLDQHP RRHGDRTRQR PGQTVGPGLR MGTGATHVPA CGAGGRIADV TRGGPLHPGR PQPGTAADLR NGRRNAEFHA PR
Validated NT Sequence
gcttcttctt gtcgatcttg ccgaccgccg tggtgggcat ggcgggcatc ggaaccagta cgtcggggcg cgcatgcgcc gatgcgcccc gctggtcgag gaacttgttc agttccacca ggctgatcgg cgctccgttg aaaaccactg cggcgcagat cttctcgccc aggtagtcat caggcagtgc caccgctgcg gctgtgtgca ccgcagggtg gctcagcaga tggtcctcga ggtctgtggc cgacacggtc tcaccgccac ggtggatgac gtccttgatc cggcccgtga cctcgacgta gccggcccgc ggtccgtcgg tgaagatgcg tacccgatcg ccggtgcggt agaagccgtc agggctgaag gagcgggcat tggcctcctc ggcgcggtaa tagccgttga gcgtgtacgg tccgcggacc agtagttcac cctcctggcc cggggcgacg tcgtggccgt cctcgtcgac gacgcgcatc tcgtcatgcg gcgacatcgg tcggccctgg gtgttctcca ccacgtccac cggatcaccg gggcgcgtga aattcagcat tccttcggcc attccgaaga tctgctgcag tcccgggctg aggccttccc ggatgaagcg ggcctcctcg ggtgacatcc gcgatccgcc cacctgcacc acacgcaggg acgtgggtag caccggttcc cattcgcagg cctgggccca cagtttggcc agggcgttga cgagtccggt caccgtgacg ccgtgggtgt tgatcaaggc gaaagctgat tctggacnng gnatcgtcgg tgtagaccgt tgtggctccg accgtcatcg agccgagcag gcccgggcac gcgaacggga agttgtggcc ggccggcaac accgccagat agacgtcgtc atgcaccatc tcgcacgcct gggcgcaggc cgcggcgttg tagaggtgat cgtcgtgagt gcgggggatg agcttcggca gcccggtggt tccgccggac accagcagga ctgcgggacc gctggtgtcg acggtgatgt cgggaggccc cggattctcg aaatccttga gcgctgacca ggattgaaag ctgccgggtt cgccgtcgac gaggatgtgg cgcagtcgcg gatgctcgga cacgagttcg gcggccaggt cgcggtagtc gaatccggcg atgacgtcgg ggatcacgag tccgacggcg ccgctgacct cggcgaagtg gcccaactcg gcagaccggt gcccgggtag gcacagcacg ggcaccacgc cggcgcgaag caaaccgaaa gtggccaccg cgaattcgcg cgagttcggc agttgcacca ggacgcggtc gccctgatgg attccctggg cggcgagtgc ggcaccgatg cggtcggcga gcgcatccaa ttcggcgaaa ctgtaggtgc ctgaggcgtc gacgacggcc gccttgtcgg gccagcgttg cgcagcctcg cgaaggatgc tgtccagtgc tttgccttgc cagtagccgg cctgccggta tacctcggca cgggcttcgg gaaaccgggt gaatcccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHGF TRFPEARAEV YRQAGYWQGK ALDSILREAA QRWPDKAAVV DASGTYSFAE LDALADRIGA ALAAQGIHQG DRVLVQLPNS REFAVATFGL LRAGVVPVLC LPGHRSAELG HFAEVSGAVG LVIPDVIAGF DYRDLAAELV SEHPRLRHIL VDGEPGSFQS WSALKDFENP GPPDITVDTS GPAVLLVSGG TTGLPKLIPR THDDYLYNAA ACAQACEMVH DDVYLAVLPA GHNFPFACPG LLGSMTVGAT TVYTDDPSPE SAFALINTHG VTVTGLVNAL AKLWAQACEW EPVLPTSLRV VQVGGSRMSP EEARFIREGL SPGLQQIFGM AEGMLNFTRP GDPVDVVENT QGRPMSPHDE MRVVDEDGHD VAPGQEGELL VRGPYTLNGY YRAEEANARS FSPDGFYRTG DRVRIFTDGP RAGYVEVTGR IKDVIHRGGE TVSATDLEDH LLSHPAVHTA AAVALPDDYL GEKICAAVVF NGAPISLVEL NKFLDQRGAS AHARPDVLVP MPAMPTTAVG KIDKKKLIAA ITER
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggattca cccggtttcc cgaagcccgt gccgaggtat accggcaggc cggctactgg caaggcaaag cactggacag catccttcgc gaggctgcgc aacgctggcc cgacaaggcg gccgtcgtcg acgcctcagg cacctacagt ttcgccgaat tggatgcgct cgccgaccgc atcggtgccg cactcgccgc ccagggaatc catcagggcg accgcgtcct ggtgcaactg ccgaactcgc gcgaattcgc ggtggccact ttcggtttgc ttcgcgccgg cgtggtgccc gtgctgtgcc tacccgggca ccggtctgcc gagttgggcc acttcgccga ggtcagcggc gccgtcggac tcgtgatccc cgacgtcatc gccggattcg actaccgcga cctggccgcc gaactcgtgt ccgagcatcc gcgactgcgc cacatcctcg tcgacggcga acccggcagc tttcaatcct ggtcagcgct caaggatttc gagaatccgg ggcctcccga catcaccgtc gacaccagcg gtcccgcagt cctgctggtg tccggcggaa ccaccgggct gccgaagctc atcccccgca ctcacgacga ttacctctac aacgccgcgg cctgcgccca ggcgtgcgag atggtgcatg acgacgtcta tctggcggtg ttgccggccg gccacaactt cccgttcgcg tgcccgggcc tgctcggctc gatgacggtc ggagccacaa cggtctacac cgacgatccc agtccagaat cagctttcgc cttgatcaac acccacggcg tcacggtgac cggactcgtc aacgccctgg ccaaactgtg ggcccaggcc tgcgaatggg aaccggtgct acccacgtcc ctgcgtgtgg tgcaggtggg cggatcgcgg atgtcacccg aggaggcccg cttcatccgg gaaggcctca gcccgggact gcagcagatc ttcggaatgg ccgaaggaat gctgaatttc acgcgccccg gtgatccggt ggacgtggtg gagaacaccc agggccgacc gatgtcgccg catgacgaga tgcgcgtcgt cgacgaggac ggccacgacg tcgccccggg ccaggagggt gaactactgg tccgcggacc gtacacgctc aacggctatt accgcgccga ggaggccaat gcccgctcct tcagccctga cggcttctac cgcaccggcg atcgggtacg catcttcacc gacggaccgc gggccggcta cgtcgaggtc acgggccgga tcaaggacgt catccaccgt ggcggtgaga ccgtgtcggc cacagacctc gaggaccatc tgctgagcca ccctgcggtg cacacagccg cagcggtggc actgcctgat gactacctgg gcgagaagat ctgcgccgca gtggttttca acggagcgcc gatcagcctg gtggaactga acaagttcct cgaccagcgg ggcgcatcgg cgcatgcgcg ccccgacgta ctggttccga tgcccgccat gcccaccacg gcggtcggca agatcgacaa gaagaagctg atcgccgcaa tcaccgagcg atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat