MyfoA.00612.a

Lysine--tRNA ligase LysS (EC 6.1.1.6) (Lysyl-tRNA synthetase)

CENTER ID: MyfoA.00612.a
ORGANISM: Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyfoA.00612.a.B1.GE40750 full length 1 507
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1214102.3.peg.5470
UniProt: K0UI86

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPADRDDAS QSQADLPEQF RIRQAKRERL LAEGREPYPV TVPRTHTLAE LRSAYPDLAA DTQTGVTVGV SGRVVFARNS GKLCFATLQE GDGTQLQAMI SLAQVGQESL DAWKADVDLG DIVFVHGEVI SSKRGELSVL ADSWQMAAKA LRPLPVAHKE MSEEARVRQR YVDLIVRPEA RSIARQRVAV VRAVRSALER RGFLEVETPM LQTLAGGAAA RPFITHSNAL DVDLYLRIAP ELFLKRCIVG GFDRVFELNR NFRNEGVDST HSPEFSMLET YQAYGDYNDS AIVTRELIQE VADEAIGTRQ VPLPDGTIYD LDGQWDTLEM YPSLSEALGE EITPETSVEY LWRIADRLEV EIPRDRGYGH GKLIEELWEH AVGEKLWAPT FVRDFPVETS PLTRAHRSIP GVTEKWDLYI RRFELATGYS ELVDPVIQRE RFEAQARAAA AGDDEAMVLD DDFLAALEYA MPPTTGTGMG IDRLLMALTG LSIRETVLFP IVRRHSN
NT Sequence
gtgagcccag ccgaccgcga tgacgcgtcc cagtcccagg ccgaccttcc cgaacagttc cggattcgtc aggccaagcg ggagcggctg ctggctgagg gccgcgaacc ctatccggtg accgtcccgc gaacccatac tctcgccgaa ctgcgcagtg cctatcccga cctggccgcc gacacccaga ccggggtgac cgtcggtgtc tcgggtcgcg tcgtgttcgc ccgcaactcg ggcaagctgt gtttcgccac gctgcaggag ggcgacggca cacagctgca ggcgatgatc agcctggccc aggtggggca ggagtcgctc gacgcgtgga aggccgacgt ggacctcggc gacatcgtct tcgttcacgg tgaggtgatc agttccaagc gcggtgaact ctcggtgctg gccgattcgt ggcagatggc ggccaaggcg ctgcgtccgc ttccggttgc tcacaaggag atgagcgaag aagcgcgcgt tcgtcagcgt tacgtggatc tcatcgtgcg tccggaggcg cgcagcatcg cgcgtcagcg ggtggcggtc gtgcgggcgg ttcgctcggc gctggaacgt cggggcttcc tcgaggtcga gacgccgatg ttgcagacgc tggccggtgg agcggcggcc cggccgttca ttacccactc gaatgcgctc gatgtggatc tgtacctgcg tatcgctccg gaactgttcc tgaagcgctg catcgtcggc ggttttgacc gggttttcga gttgaatcgg aactttcgta acgaaggtgt tgattcgacg cactcaccgg aattctcgat gttggagaca tatcaggcgt acggcgacta caacgattcc gcgatagtca cacgcgaact tattcaagag gtcgccgacg aggcgatcgg tacccgtcag gtgccattgc ctgatggcac tatctacgac ctcgacgggc aatgggatac attggaaatg tatccctcgt tgtccgaggc tctgggtgag gagatcacgc cggagacttc ggtggagtac ttgtggcgta ttgctgatcg actcgaggtc gagattccgc gggaccgtgg ctacggacac ggaaaattga tcgaggaact ctgggaacat gccgtcggcg agaagctctg ggcccctacg ttcgtccgcg acttcccggt cgagacctcg ccgctgaccc gcgctcaccg cagcattccg ggtgtgaccg agaagtggga cctctacata cgcagattcg agctcgccac gggatattcg gaactcgtcg atccggtaat ccaacgggaa cgcttcgagg cacaggctcg tgcggctgcc gccggagatg acgaggcaat ggttctcgac gacgatttcc ttgccgcatt ggagtacgcg atgccgccga ccaccggcac cggaatgggt atcgatcgct tgttgatggc gttgacgggg ttgtctatta gagagacagt tttgttcccg attgttcgcc gtcacagtaa ctga
Details for MyfoA.00612.a.B1.GE40750
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 58kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV SPADRDDASQ SQADLPEQFR IRQAKRERLL AEGREPYPVT VPRTHTLAEL RSAYPDLAAD TQTGVTVGVS GRVVFARNSG KLCFATLQEG DGTQLQAMIS LAQVGQESLD AWKADVDLGD IVFVHGEVIS SKRGELSVLA DSWQMAAKAL RPLPVAHKEM SEEARVRQRY VDLIVRPEAR SIARQRVAVV RAVRSALERR GFLEVETPML QTLAGGAAAR PFITHSNALD VDLYLRIAPE LFLKRCIVGG FDRVFELNRN FRNEGVDSTH SPEFSMLETY QAYGDYNDSA IVTRELIQEV ADEAIGTRQV PLPDGTIYDL DGQWDTLEMY PSLSEALGEE ITPETSVEYL WRIADRLEVE IPRDRGYGHG KLIEELWEHA VGEKLWAPTF VRDFPVETSP LTRAHRSIPG VTEKWDLYIR RFELATGYSE LVDPVIQRER FEAQARAAAA GDDEAMVLDD DFLAALEYAM PPTTGTGMGI DRLLMALTGL SIRETVLF
Validated NT Sequence
cgggaacaaa actgtctctc taatagacaa ccccgtcaac gccatcaaca agcgatcgat acccattccg gtgccggtgg tcggcggcat cgcgtactcc aatgcggcaa ggaaatcgtc gtcgagaacc attgcctcgt catctccggc ggcagccgca cgagcctgtg cctcgaagcg ttcccgttgg attaccggat cgacgagttc cgaatatccc gtggcgagct cgaatctgcg tatgtagagg tcccacttct cggtcacacc cggaatgctg cggtgagcgc gggtcagcgg cgaggtctcg accgggaagt cgcggacgaa cgtaggggcc cagagcttct cgccgacggc atgttcccag agttcctcga tcaattttcc gtgtccgtag ccacggtccc gcggaatctc gacctcgagt cgatcagcaa tacgccacaa gtactccacc gaagtctccg gcgtgatctc ctcacccaga gcctcggaca acgagggata catttccaat gtatcccatt gcccgtcgag gtcgtagata gtgccatcag gcaatggcac ctgacgggta ccgatcgcct cgtcggcgac ctcttgaata agttcgcgtg tgactatcgc ggaatcgttg tagtcgccgt acgcctgata tgtctccaac atcgagaatt ccggtgagtg cgtcgaatca acaccttcgt tacgaaagtt ccgattcaac tcgaaaaccc ggtcaaaacc gccgacgatg cagcgcttca ggaacagttc cggagcgata cgcaggtaca gatccacatc gagcgcattc gagtgggtaa tgaacggccg ggccgccgct ccaccggcca gcgtctgcaa catcggcgtc tcgacctcga ggaagccccg acgttccagc gccgagcgaa ccgcccgcac gaccgccacc cgctgacgcg cgatgctgcg cgcctccgga cgcacgatga gatccacgta acgctgacga acgcgcgctt cttcgctcat ctccttgtga gcaaccggaa gcggacgcag cgccttggcc gccatctgcc acgaatcggc cagcaccgag agttcaccgc gcttggaact gataacctca ccgtgaacga agacgatgtc gccgaggtcc acgtcggcct tccacgcgtc gagcgactcc tgccccacct gggccaggct gatcatcgcc tgcagctgtg tgccgtcgcc ctcctgcagc gtggcgaaac acagcttgcc cgagttgcgg gcgaacacga cgcgacccga gacaccgacg gtcaccccgg tctgggtgtc ggcggccagg tcgggatagg cactgcgcag ttcggcgaga gtatgggttc gcgggacggt caccggatag ggttcgcggc cctcagccag cagccgctcc cgcttggcct gacgaatccg gaactgttcg ggaaggtcgg cctgggactg ggacgcgtca tcgcggtcgg ctgggctcac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS PADRDDASQS QADLPEQFRI RQAKRERLLA EGREPYPVTV PRTHTLAELR SAYPDLAADT QTGVTVGVSG RVVFARNSGK LCFATLQEGD GTQLQAMISL AQVGQESLDA WKADVDLGDI VFVHGEVISS KRGELSVLAD SWQMAAKALR PLPVAHKEMS EEARVRQRYV DLIVRPEARS IARQRVAVVR AVRSALERRG FLEVETPMLQ TLAGGAAARP FITHSNALDV DLYLRIAPEL FLKRCIVGGF DRVFELNRNF RNEGVDSTHS PEFSMLETYQ AYGDYNDSAI VTRELIQEVA DEAIGTRQVP LPDGTIYDLD GQWDTLEMYP SLSEALGEEI TPETSVEYLW RIADRLEVEI PRDRGYGHGK LIEELWEHAV GEKLWAPTFV RDFPVETSPL TRAHRSIPGV TEKWDLYIRR FELATGYSEL VDPVIQRERF EAQARAAAAG DDEAMVLDDD FLAALEYAMP PTTGTGMGID RLLMALTGLS IRETVLFPIV RRHSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagcc cagccgaccg cgatgacgcg tcccagtccc aggccgacct tcccgaacag ttccggattc gtcaggccaa gcgggagcgg ctgctggctg agggccgcga accctatccg gtgaccgtcc cgcgaaccca tactctcgcc gaactgcgca gtgcctatcc cgacctggcc gccgacaccc agaccggggt gaccgtcggt gtctcgggtc gcgtcgtgtt cgcccgcaac tcgggcaagc tgtgtttcgc cacgctgcag gagggcgacg gcacacagct gcaggcgatg atcagcctgg cccaggtggg gcaggagtcg ctcgacgcgt ggaaggccga cgtggacctc ggcgacatcg tcttcgttca cggtgaggtg atcagttcca agcgcggtga actctcggtg ctggccgatt cgtggcagat ggcggccaag gcgctgcgtc cgcttccggt tgctcacaag gagatgagcg aagaagcgcg cgttcgtcag cgttacgtgg atctcatcgt gcgtccggag gcgcgcagca tcgcgcgtca gcgggtggcg gtcgtgcggg cggttcgctc ggcgctggaa cgtcggggct tcctcgaggt cgagacgccg atgttgcaga cgctggccgg tggagcggcg gcccggccgt tcattaccca ctcgaatgcg ctcgatgtgg atctgtacct gcgtatcgct ccggaactgt tcctgaagcg ctgcatcgtc ggcggttttg accgggtttt cgagttgaat cggaactttc gtaacgaagg tgttgattcg acgcactcac cggaattctc gatgttggag acatatcagg cgtacggcga ctacaacgat tccgcgatag tcacacgcga acttattcaa gaggtcgccg acgaggcgat cggtacccgt caggtgccat tgcctgatgg cactatctac gacctcgacg ggcaatggga tacattggaa atgtatccct cgttgtccga ggctctgggt gaggagatca cgccggagac ttcggtggag tacttgtggc gtattgctga tcgactcgag gtcgagattc cgcgggaccg tggctacgga cacggaaaat tgatcgagga actctgggaa catgccgtcg gcgagaagct ctgggcccct acgttcgtcc gcgacttccc ggtcgagacc tcgccgctga cccgcgctca ccgcagcatt ccgggtgtga ccgagaagtg ggacctctac atacgcagat tcgagctcgc cacgggatat tcggaactcg tcgatccggt aatccaacgg gaacgcttcg aggcacaggc tcgtgcggct gccgccggag atgacgaggc aatggttctc gacgacgatt tccttgccgc attggagtac gcgatgccgc cgaccaccgg caccggaatg ggtatcgatc gcttgttgat ggcgttgacg gggttgtcta ttagagagac agttttgttc ccgattgttc gccgtcacag taactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat