SSGCID: Target Page

MyfoA.00211.a

Pyrroline-5-carboxylate reductase (EC 1.5.1.2) (Rv0500 ortholog)

CENTER ID:	MyfoA.00211.a
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.00211.a.B1.GE37894	full length		1	275

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1214102.3.peg.1762
UniProt:	K0VAA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGEALLAGLL RAGRQVKDMV VAEKYPDRAK YLADKYSVLV TSVADAADNA AYVVIAVKPG DVANVIDEIA DAAARADSDT AEQVFVTIAA GVSTSYYENK LPAGSPVVRV MPNSPIVVGG GVSALAPGRF ATAEQLKEVS SIFDAVGGVI TVAESQLDAV TAVSGSGPAY FFLMVEALVD AGVESGLSRA VATDLAVQTM AGSAAMLLER LDEVNAAGGA ALDTTPARLR AMVTSPAGTT AAGLRELERG GLRGAVSNAV QAAKTRSEQL GITSE

NT Sequence

atgggcgagg cactgctggc agggctgtta cgggccggcc ggcaggtcaa ggacatggtg gtcgcggaga agtacccgga ccgggccaag tatctggcgg acaagtattc ggtgctggtg acctcggtcg ccgatgccgc ggacaatgcc gcctacgtgg tgatcgcggt caagccgggc gatgtcgcga atgtgatcga cgagatcgcc gacgccgcgg cccgtgccga ttcggacacc gccgagcagg tgttcgtcac gatcgcagcc ggtgtgagca cctcgtacta cgagaacaag ctgccggccg gctcgccggt cgtccgggtc atgcccaact cgccgatcgt ggtcggcggc ggggtgagcg cgttggcccc gggccggttc gccactgccg agcagctcaa agaggtctcg tcgatcttcg atgcggtcgg tggcgtgatc accgtcgcgg agtcccagct ggacgcggtg accgcggtgt ccgggtcggg cccggcgtac ttcttcctga tggtcgaggc tctggtcgat gcgggcgtgg aatcgggtct gtcgcgcgcg gtggccaccg atctggcggt ccagacgatg gccggctcgg ccgcgatgct gctggaacgt ctcgacgagg tgaacgcggc cggaggggcc gcgctggaca ccacaccggc ccgattgcgg gcgatggtga cctctccggc cggtaccacc gccgctgggc tgcgggaact cgagcgtggg ggattgcggg gtgcggtctc caatgccgtt caggccgcga aaacgcgctc tgagcagctc ggaattacat ctgagtaa

Details for MyfoA.00211.a.B1.GE37894

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG EALLAGLLRA GRQVKDMVVA EKYPDRAKYL ADKYSVLVTS VADAADNAAY VVIAVKPGDV ANVIDEIADA AARADSDTAE QVFVTIAAGV STSYYENKLP AGSPVVRVMP NSPIVVGGGV SALAPGRFAT AEQLKEVSSI FDAVGGVITV AESQLDAVTA VSGSGPAYFF LMVEALVDAG VESGLSRAVA TDLAVQTMAG SAAMLLERLD EVNAAGGAAL DTTPARLRAM VTSPAGTTAA GLRELERGGL RGAVSNAVXP RKRALSSSEL HLSK

Validated NT Sequence

cnctngnnta attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatggg cgaggcactg ctggcagggc tgttacgggc cggccggcag gtcaaggaca tggtggtcgc ggagaagtac ccggaccggg ccaagtatct ggcggacaag tattcggtgc tggtgacctc ggtcgccgat gccgcggaca atgccgccta cgtggtgatc gcggtcaagc cgggcgatgt cgcgaatgtg atcgacgaga tcgccgacgc cgcggcccgt gccgattcgg acaccgccga gcaggtgttc gtcacgatcg cagccggtgt gagcacctcg tactacgaga acaagctgcc ggccggctcg ccggtcgtcc gggtcatgcc caactcgccg atcgtggtcg gcggcggggt gagcgcgttg gccccgggcc ggttcgccac tgccgagcag ctcaaagagg tctcgtcgat cttcgatgcg gtcggtggcg tgatcaccgt cgcggagtcc cagctggacg cggtgaccgc ggtgtccggg tcgggcccgg cgtacttctt cctgatggtc gaggctctgg tcgatgcggg cgtggaatcg ggtctgtcgc gcgcggtggc caccgatctg gcggtccaga cgatggccgg ctcggccgcg atgctgctgg aacgtctcga cgaggtgaac gcggccggag gggccgcgct ggacaccaca ccggcccgat tgcgggcgat ggtgacctct ccggccggta ccaccgccgc tgggctgcgg gaactcgagc gtgggggatt gcggggtgcg gtctccaatg ccgttcngcc gcgaaaacgc gctctgagca gctcggaatt acatctgagt aagtgagtaa gataggatcc nntgtnnnna ccccccccna ganaannnnn ngannnnnng tgnnngcnng ccatnannnn ngctngcgan tgtanaggnn nntnnnacta nt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg aggcactgct ggcagggctg ttacgggccg gccggcaggt caaggacatg gtggtcgcgg agaagtaccc ggaccgggcc aagtatctgg cggacaagta ttcggtgctg gtgacctcgg tcgccgatgc cgcggacaat gccgcctacg tggtgatcgc ggtcaagccg ggcgatgtcg cgaatgtgat cgacgagatc gccgacgccg cggcccgtgc cgattcggac accgccgagc aggtgttcgt cacgatcgca gccggtgtga gcacctcgta ctacgagaac aagctgccgg ccggctcgcc ggtcgtccgg gtcatgccca actcgccgat cgtggtcggc ggcggggtga gcgcgttggc cccgggccgg ttcgccactg ccgagcagct caaagaggtc tcgtcgatct tcgatgcggt cggtggcgtg atcaccgtcg cggagtccca gctggacgcg gtgaccgcgg tgtccgggtc gggcccggcg tacttcttcc tgatggtcga ggctctggtc gatgcgggcg tggaatcggg tctgtcgcgc gcggtggcca ccgatctggc ggtccagacg atggccggct cggccgcgat gctgctggaa cgtctcgacg aggtgaacgc ggccggaggg gccgcgctgg acaccacacc ggcccgattg cgggcgatgg tgacctctcc ggccggtacc accgccgctg ggctgcggga actcgagcgt gggggattgc ggggtgcggt ctccaatgcc gttcaggccg cgaaaacgcg ctctgagcag ctcggaatta catctgagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 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