MyfoA.00170.a: Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) (InhA)
Target Characteristics
from organism:
Mycobacterium fortuitum ATCC 6841 / DSM 46621
most recent status:
in PDB
center reference id:
MyfoA.00170.a
is community request:
True
associated disease:
NIH risk group:
2
is select agent:
False
NIH priority
IIIC
Ordering Clones & Proteins
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Clones*
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
MyfoA.00170.a.B1.GE42832
full length
more 1
269
GE42832
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER
DOMAIN/REGION
INFO
AA
AA
ORDER
MyfoA.00170.a.B1.PS38584
full length
more 1
269
PS38584
Structures
7K73
Deposited: 9/22/2020
Determination: XRayDiffraction Image
7U0O
Deposited: 2/18/2022
Determination: XRayDiffraction Image
Publications by SSGCID
In vitro and in
vivo efficacy of NITD-916 against Mycobacterium
fortuitum
Abendroth J, Edwards TE, Kremer L, Alcaraz M, Roquet-Banères F, Hamela C
Antimicrob. Agents Chemother.
External Resources Enzyme & Pathway Information
Pathway
Pathway ID
EC Number
biotin biosynthesis I
BIOTIN-BIOSYNTHESIS-PWY 1.3.1.9
fatty acid elongation -- saturated
FASYN-ELONG-PWY 1.3.1.9
(5Z)-dodecenoate biosynthesis I
PWY0-862 1.3.1.9
superpathway of fatty acid biosynthesis I (E. coli)
PWY0-881 1.3.1.9
superpathway of fatty acid biosynthesis II (plant)
PWY-5156 1.3.1.9
palmitate biosynthesis II (bacteria and plants)
PWY-5971 1.3.1.9
cis-vaccenate biosynthesis
PWY-5973 1.3.1.9
stearate biosynthesis II (bacteria and plants)
PWY-5989 1.3.1.9
superpathway of mycolate biosynthesis
PWY-6113 1.3.1.9
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)
PWY-6282 1.3.1.9
superpathway of unsaturated fatty acids biosynthesis (E. coli)
PWY-6284 1.3.1.9
superpathway of fatty acids biosynthesis (E. coli)
PWY-6285 1.3.1.9
8-amino-7-oxononanoate biosynthesis I
PWY-6519 1.3.1.9
triclosan resistance
PWY-7096 1.3.1.9
gondoate biosynthesis (anaerobic)
PWY-7663 1.3.1.9
oleate biosynthesis IV (anaerobic)
PWY-7664 1.3.1.9
(5Z)-dodecenoate biosynthesis II
PWY-7858 1.3.1.9
mycolate biosynthesis
PWYG-321 1.3.1.9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "more" button next to the name of that material.
AA Sequence
MAGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRMKLIQRI ADRLPKPAPL LELDVQNEEH LASLADRISG AIGEGNKLDG VVHSIGFMPQ TGMGVNPFFD APYADVAKGI HISAYSYASL AKATLPIMNE GGSIVGMDFD PTRAMPAYNW MTVAKSALES VNRFVAREAG AAGVRSNLVA AGPIRTLAMS AIVGGALGDE AGKQMQLLEE GWDQRAPIGW NMKDPTPVAK TVCALLSDWM PATTGTIIYA DGGASTQLL
NT Sequence
atggcaggtt tgctcgaagg caagcgcatc ctcgtcaccg ggatcatcac ggactcgtcg atcgcattcc acatcgcgaa ggtggcccag gaagccgggg ctgagctggt gctcaccggg ttcgaccgga tgaagctgat ccagcgcatc gccgaccggt tgcccaaacc ggcgccgctg ctggaactcg acgtgcagaa cgaggagcac ctcgcgagcc tggccgaccg gatctcgggc gccatcggcg agggcaacaa gctcgacggc gtggtccact ccatcggctt catgccgcag acaggcatgg gggtgaaccc cttcttcgac gccccgtacg ccgatgtcgc caaggggatc cacatctcgg cctactccta cgcctcgctg gccaaggcca cgctgccgat catgaacgag ggcggcagca tcgtcggcat ggacttcgat ccgacgcgcg ccatgccggc atacaactgg atgacggtgg ccaagagcgc tctggagtcg gtcaaccggt tcgtggctcg cgaggccggc gcggccggtg tccgttcgaa tctcgttgcc gccggcccga tccggactct cgccatgagc gccatcgtcg gcggcgcact gggtgacgag gcgggcaagc agatgcagct gctggaagag ggctgggatc agcgtgcgcc gatcggctgg aacatgaagg atccgacgcc ggtcgccaag accgtgtgtg cgttgctgtc cgactggatg cccgccacca caggcaccat catctacgcc gacggcggcg ccagcaccca gttgctctga
purified on: 5/21/2019
concentration: 28.49mg/ml
observed molecular weight:
29kDa
expression level:
Moderate Expression
protein purification buffer:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
expression host:
BL 21 (DE3) Rosetta
vial count (approx.):
14
vial volume:
200µl
percent identity: 98
percent coverage: 100
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEA GAELVLTGFD RMKLIQRIAD RLPKPAPLLE LDVQNEEHLA SLADRISGAI GEGNKLDGVV HSIGFMPQTG MGVNPFFDAP YADVAKGIHI SAYSYASLAK ATLPIMNEGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGAA GVRSNLVAAG PIRTLAMSAI VGGALGDEAG KQMQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWMPA TTGTIIYAXX XASTQLL
TCTTACTCAC TTAGAGCAAC TGGGTGCTGG CGCNNNNNNN GGCGTAGATG ATGGTGCCTG TGGTGGCGGG CATCCAGTCG GACAGCAACG CACACACGGT CTTGGCGACC GGCGTCGGAT CCTTCATGTT CCAGCCGATC GGCGCACGCT GATCCCAGCC CTCTTCCAGC AGCTGCATCT GCTTGCCCGC CTCGTCACCC AGTGCGCCGC CGACGATGGC GCTCATGGCG AGAGTCCGGA TCGGGCCGGC GGCGACGAGA TTCGAACGGA CACCGGCCGC GCCGGCCTCG CGAGCCACGA ACCGGTTGAC CGACTCCAGA GCGCTCTTGG CCACCGTCAT CCAGTTGTAT GCCGGCATGG CGCGCGTCGG ATCGAAGTCC ATGCCGACGA TGCTGCCGCC CTCGTTCATG ATCGGCAGCG TGGCCTTGGC CAGCGAGGCG TAGGAGTAGG CCGAGATGTG GATCCCCTTG GCGACATCGG CGTACGGGGC GTCGAAGAAG GGGTTCACCC CCATGCCTGT CTGCGGCATG AAGCCGATGG AGTGGACCAC GCCGTCGAGC TTGTTGCCCT CGCCGATGGC GCCCGAGATC CGGTCGGCCA GGCTCGCGAG GTGCTCCTCG TTCTGCACGT CGAGCTCCAG CAGCGGCGCC GGTTTGGGCA ACCGGTCGGC GATGCGCTGG ATCAGCTTCA TCCGGTCGAA CCCGGTGAGC ACCAGCTCAG CCCCGGCTTC CTGGGCCACC TTCGCGATGT GGAATGCGAT CGACGAGTCC GTGATGATCC CGGTGACGAG GATGCGCTTG CCTTCGAGCA AACCTGCCAT ATGGTGGTGG TGGTGGTGAG CCAT
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEA GAELVLTGFD RMKLIQRIAD RLPKPAPLLE LDVQNEEHLA SLADRISGAI GEGNKLDGVV HSIGFMPQTG MGVNPFFDAP YADVAKGIHI SAYSYASLAK ATLPIMNEGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGAA GVRSNLVAAG PIRTLAMSAI VGGALGDEAG KQMQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWMPA TTGTIIYADG GASTQLL
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG GTTTGCTCGA AGGCAAGCGC ATCCTCGTCA CCGGGATCAT CACGGACTCG TCGATCGCAT TCCACATCGC GAAGGTGGCC CAGGAAGCCG GGGCTGAGCT GGTGCTCACC GGGTTCGACC GGATGAAGCT GATCCAGCGC ATCGCCGACC GGTTGCCCAA ACCGGCGCCG CTGCTGGAAC TCGACGTGCA GAACGAGGAG CACCTCGCGA GCCTGGCCGA CCGGATCTCG GGCGCCATCG GCGAGGGCAA CAAGCTCGAC GGCGTGGTCC ACTCCATCGG CTTCATGCCG CAGACAGGCA TGGGGGTGAA CCCCTTCTTC GACGCCCCGT ACGCCGATGT CGCCAAGGGG ATCCACATCT CGGCCTACTC CTACGCCTCG CTGGCCAAGG CCACGCTGCC GATCATGAAC GAGGGCGGCA GCATCGTCGG CATGGACTTC GATCCGACGC GCGCCATGCC GGCATACAAC TGGATGACGG TGGCCAAGAG CGCTCTGGAG TCGGTCAACC GGTTCGTGGC TCGCGAGGCC GGCGCGGCCG GTGTCCGTTC GAATCTCGTT GCCGCCGGCC CGATCCGGAC TCTCGCCATG AGCGCCATCG TCGGCGGCGC ACTGGGTGAC GAGGCGGGCA AGCAGATGCA GCTGCTGGAA GAGGGCTGGG ATCAGCGTGC GCCGATCGGC TGGAACATGA AGGATCCGAC GCCGGTCGCC AAGACCGTGT GTGCGTTGCT GTCCGACTGG ATGCCCGCCA CCACAGGCAC CATCATCTAC GCCGACGGCG GCGCCAGCAC CCAGTTGCTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
