MyfoA.00170.a
Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) (InhA)
| CENTER ID: | MyfoA.00170.a |
| ORGANISM: | Mycobacterium fortuitum ATCC 6841 / DSM 46621 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyfoA.00170.a.B1.GE42832 | full length | 1 | 269 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyfoA.00170.a.B1.PS38584 | full length | 1 | 269 |
Structures
7K73
DEPOSITED: 9/22/2020
DETERMINATION: XRay
CLONE: MyfoA.00170.a.B1.GE42832
PROTEIN: MyfoA.00170.a.B1.PS38584
7U0O
DEPOSITED: 2/18/2022
DETERMINATION: XRay
CLONE: MyfoA.00170.a.B1.GE42832
PROTEIN: MyfoA.00170.a.B1.PS38584
Publications by SSGCID
In vitro and in vivo efficacy of NITD-916 against Mycobacterium fortuitum
Abendroth J, Edwards TE, Kremer L, Alcaraz M, Roquet-Banères F, Hamela C
Antimicrob. Agents Chemother. - 2023
PMID: 36920188; PMCID: PMC10112203
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1766.6.peg.2938 |
| UniProt: | A0A0N9XSE6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGLLEGKRI LVTGIITDSS IAFHIAKVAQ EAGAELVLTG FDRMKLIQRI ADRLPKPAPL LELDVQNEEH LASLADRISG AIGEGNKLDG VVHSIGFMPQ TGMGVNPFFD APYADVAKGI HISAYSYASL AKATLPIMNE GGSIVGMDFD PTRAMPAYNW MTVAKSALES VNRFVAREAG AAGVRSNLVA AGPIRTLAMS AIVGGALGDE AGKQMQLLEE GWDQRAPIGW NMKDPTPVAK TVCALLSDWM PATTGTIIYA DGGASTQLL
NT Sequence
atggcaggtt tgctcgaagg caagcgcatc ctcgtcaccg ggatcatcac ggactcgtcg atcgcattcc acatcgcgaa ggtggcccag gaagccgggg ctgagctggt gctcaccggg ttcgaccgga tgaagctgat ccagcgcatc gccgaccggt tgcccaaacc ggcgccgctg ctggaactcg acgtgcagaa cgaggagcac ctcgcgagcc tggccgaccg gatctcgggc gccatcggcg agggcaacaa gctcgacggc gtggtccact ccatcggctt catgccgcag acaggcatgg gggtgaaccc cttcttcgac gccccgtacg ccgatgtcgc caaggggatc cacatctcgg cctactccta cgcctcgctg gccaaggcca cgctgccgat catgaacgag ggcggcagca tcgtcggcat ggacttcgat ccgacgcgcg ccatgccggc atacaactgg atgacggtgg ccaagagcgc tctggagtcg gtcaaccggt tcgtggctcg cgaggccggc gcggccggtg tccgttcgaa tctcgttgcc gccggcccga tccggactct cgccatgagc gccatcgtcg gcggcgcact gggtgacgag gcgggcaagc agatgcagct gctggaagag ggctgggatc agcgtgcgcc gatcggctgg aacatgaagg atccgacgcc ggtcgccaag accgtgtgtg cgttgctgtc cgactggatg cccgccacca caggcaccat catctacgcc gacggcggcg ccagcaccca gttgctctga
Details for MyfoA.00170.a.B1.GE42832
| HARVESTED ON: | 1/9/2019 |
| SEQUENCED ON: | 1/15/2019 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEA GAELVLTGFD RMKLIQRIAD RLPKPAPLLE LDVQNEEHLA SLADRISGAI GEGNKLDGVV HSIGFMPQTG MGVNPFFDAP YADVAKGIHI SAYSYASLAK ATLPIMNEGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGAA GVRSNLVAAG PIRTLAMSAI VGGALGDEAG KQMQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWMPA TTGTIIYAXX XASTQLL
Validated NT Sequence
tcttactcac ttagagcaac tgggtgctgg cgcnnnnnnn ggcgtagatg atggtgcctg tggtggcggg catccagtcg gacagcaacg cacacacggt cttggcgacc ggcgtcggat ccttcatgtt ccagccgatc ggcgcacgct gatcccagcc ctcttccagc agctgcatct gcttgcccgc ctcgtcaccc agtgcgccgc cgacgatggc gctcatggcg agagtccgga tcgggccggc ggcgacgaga ttcgaacgga caccggccgc gccggcctcg cgagccacga accggttgac cgactccaga gcgctcttgg ccaccgtcat ccagttgtat gccggcatgg cgcgcgtcgg atcgaagtcc atgccgacga tgctgccgcc ctcgttcatg atcggcagcg tggccttggc cagcgaggcg taggagtagg ccgagatgtg gatccccttg gcgacatcgg cgtacggggc gtcgaagaag gggttcaccc ccatgcctgt ctgcggcatg aagccgatgg agtggaccac gccgtcgagc ttgttgccct cgccgatggc gcccgagatc cggtcggcca ggctcgcgag gtgctcctcg ttctgcacgt cgagctccag cagcggcgcc ggtttgggca accggtcggc gatgcgctgg atcagcttca tccggtcgaa cccggtgagc accagctcag ccccggcttc ctgggccacc ttcgcgatgt ggaatgcgat cgacgagtcc gtgatgatcc cggtgacgag gatgcgcttg ccttcgagca aacctgccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEA GAELVLTGFD RMKLIQRIAD RLPKPAPLLE LDVQNEEHLA SLADRISGAI GEGNKLDGVV HSIGFMPQTG MGVNPFFDAP YADVAKGIHI SAYSYASLAK ATLPIMNEGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGAA GVRSNLVAAG PIRTLAMSAI VGGALGDEAG KQMQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWMPA TTGTIIYADG GASTQLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag gtttgctcga aggcaagcgc atcctcgtca ccgggatcat cacggactcg tcgatcgcat tccacatcgc gaaggtggcc caggaagccg gggctgagct ggtgctcacc gggttcgacc ggatgaagct gatccagcgc atcgccgacc ggttgcccaa accggcgccg ctgctggaac tcgacgtgca gaacgaggag cacctcgcga gcctggccga ccggatctcg ggcgccatcg gcgagggcaa caagctcgac ggcgtggtcc actccatcgg cttcatgccg cagacaggca tgggggtgaa ccccttcttc gacgccccgt acgccgatgt cgccaagggg atccacatct cggcctactc ctacgcctcg ctggccaagg ccacgctgcc gatcatgaac gagggcggca gcatcgtcgg catggacttc gatccgacgc gcgccatgcc ggcatacaac tggatgacgg tggccaagag cgctctggag tcggtcaacc ggttcgtggc tcgcgaggcc ggcgcggccg gtgtccgttc gaatctcgtt gccgccggcc cgatccggac tctcgccatg agcgccatcg tcggcggcgc actgggtgac gaggcgggca agcagatgca gctgctggaa gagggctggg atcagcgtgc gccgatcggc tggaacatga aggatccgac gccggtcgcc aagaccgtgt gtgcgttgct gtccgactgg atgcccgcca ccacaggcac catcatctac gccgacggcg gcgccagcac ccagttgctc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MyfoA.00170.a.B1.PS38584
| PURIFICATION DATe: | 5/21/2019 |
| CONCENTRATION: | 28.49mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEA GAELVLTGFD RMKLIQRIAD RLPKPAPLLE LDVQNEEHLA SLADRISGAI GEGNKLDGVV HSIGFMPQTG MGVNPFFDAP YADVAKGIHI SAYSYASLAK ATLPIMNEGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGAA GVRSNLVAAG PIRTLAMSAI VGGALGDEAG KQMQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWMPA TTGTIIYAXX XASTQLL
Validated NT Sequence
tcttactcac ttagagcaac tgggtgctgg cgcnnnnnnn ggcgtagatg atggtgcctg tggtggcggg catccagtcg gacagcaacg cacacacggt cttggcgacc ggcgtcggat ccttcatgtt ccagccgatc ggcgcacgct gatcccagcc ctcttccagc agctgcatct gcttgcccgc ctcgtcaccc agtgcgccgc cgacgatggc gctcatggcg agagtccgga tcgggccggc ggcgacgaga ttcgaacgga caccggccgc gccggcctcg cgagccacga accggttgac cgactccaga gcgctcttgg ccaccgtcat ccagttgtat gccggcatgg cgcgcgtcgg atcgaagtcc atgccgacga tgctgccgcc ctcgttcatg atcggcagcg tggccttggc cagcgaggcg taggagtagg ccgagatgtg gatccccttg gcgacatcgg cgtacggggc gtcgaagaag gggttcaccc ccatgcctgt ctgcggcatg aagccgatgg agtggaccac gccgtcgagc ttgttgccct cgccgatggc gcccgagatc cggtcggcca ggctcgcgag gtgctcctcg ttctgcacgt cgagctccag cagcggcgcc ggtttgggca accggtcggc gatgcgctgg atcagcttca tccggtcgaa cccggtgagc accagctcag ccccggcttc ctgggccacc ttcgcgatgt ggaatgcgat cgacgagtcc gtgatgatcc cggtgacgag gatgcgcttg ccttcgagca aacctgccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEA GAELVLTGFD RMKLIQRIAD RLPKPAPLLE LDVQNEEHLA SLADRISGAI GEGNKLDGVV HSIGFMPQTG MGVNPFFDAP YADVAKGIHI SAYSYASLAK ATLPIMNEGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGAA GVRSNLVAAG PIRTLAMSAI VGGALGDEAG KQMQLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWMPA TTGTIIYADG GASTQLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG GTTTGCTCGA AGGCAAGCGC ATCCTCGTCA CCGGGATCAT CACGGACTCG TCGATCGCAT TCCACATCGC GAAGGTGGCC CAGGAAGCCG GGGCTGAGCT GGTGCTCACC GGGTTCGACC GGATGAAGCT GATCCAGCGC ATCGCCGACC GGTTGCCCAA ACCGGCGCCG CTGCTGGAAC TCGACGTGCA GAACGAGGAG CACCTCGCGA GCCTGGCCGA CCGGATCTCG GGCGCCATCG GCGAGGGCAA CAAGCTCGAC GGCGTGGTCC ACTCCATCGG CTTCATGCCG CAGACAGGCA TGGGGGTGAA CCCCTTCTTC GACGCCCCGT ACGCCGATGT CGCCAAGGGG ATCCACATCT CGGCCTACTC CTACGCCTCG CTGGCCAAGG CCACGCTGCC GATCATGAAC GAGGGCGGCA GCATCGTCGG CATGGACTTC GATCCGACGC GCGCCATGCC GGCATACAAC TGGATGACGG TGGCCAAGAG CGCTCTGGAG TCGGTCAACC GGTTCGTGGC TCGCGAGGCC GGCGCGGCCG GTGTCCGTTC GAATCTCGTT GCCGCCGGCC CGATCCGGAC TCTCGCCATG AGCGCCATCG TCGGCGGCGC ACTGGGTGAC GAGGCGGGCA AGCAGATGCA GCTGCTGGAA GAGGGCTGGG ATCAGCGTGC GCCGATCGGC TGGAACATGA AGGATCCGAC GCCGGTCGCC AAGACCGTGT GTGCGTTGCT GTCCGACTGG ATGCCCGCCA CCACAGGCAC CATCATCTAC GCCGACGGCG GCGCCAGCAC CCAGTTGCTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT