SSGCID: Target Page

MyfoA.00139.a

Dephospho-CoA kinase CoaE; protein folding accessory domain-containing protein (EC 2.7.1.24)

CENTER ID:	MyfoA.00139.a
ORGANISM:	Mycobacterium fortuitum ATCC 6841 / DSM 46621
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.00139.a.B1.GE40748	full length		1	387
MyfoA.00139.a.B2.GE40749	nterm domain		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyfoA.00139.a.B1.PW38007	full length		1	387

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1214102.3.peg.2834
UniProt:	K0VQF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRIGLTGGI GAGKSTVSAT FSDLGGIIVD GDVIAREVVE PGTEGLAKLV EAFGDGILLP EGALNRPALA AVAFSDDEKR ATLNGIVHPL VAHRRSELIA AAHDDAVIIE DIPLLVESQM APMFPLVIIV NADPEIRVKR LIEYRGFTEQ DARARIAAQA TEEQRRAVAD VWLDNSGSPG AVVEQARALW HERILPFAHN LKIRQPAHRV PVVVPYDPTW PDQARRIIAR LNTACGHRAV RIDHIGSTAV PGMDAKDVID VQVTVASLDV ADELADALLT AGYPAVPGIT TDTPHDDDPA LWHKRFHASA DPGRPTNVHV RVEGRPNQRF ALMFVDWLRS NPGAQADYLA AKRRAMATPD YAVAKEPWFL DAYRRAWEWA DAKGWQP

NT Sequence

gtgcttcgca tcgggttaac cggcggtatc ggcgccggga agtcaacagt gtcggccaca ttcagcgatc tcggcggcat catcgtcgac ggcgacgtca tcgcgcgtga ggtggtcgaa ccgggaaccg aaggtctggc caagctcgtc gaggcgttcg gtgacgggat cctgctaccc gaaggggcgc tgaaccgccc ggccctggcc gcggtcgcgt tcagcgacga cgagaagcgc gccaccctga acggcatcgt ccatccgctg gtggcgcacc gccggtctga gctgatcgcg gccgcgcacg acgatgcggt gatcatcgag gacatcccac tgttggtcga atcccagatg gcgccgatgt tcccgctggt catcatcgtc aacgccgacc cggagattcg cgtgaagcgg ctgatcgaat accgcggatt caccgagcaa gacgcccgcg cccgcatcgc cgcgcaggcc accgaggagc agcgccgcgc ggtcgccgac gtatggctgg acaactcggg cagcccaggg gccgtcgtcg aacaggctcg cgcgctctgg cacgagcgga tcctgccctt cgcgcacaac ctgaagatcc gtcagccggc tcatcgcgtc cctgttgtgg tgccgtatga cccgacctgg ccggatcagg cgcgccgtat catcgcgcgg ctcaatacag cctgcggaca ccgtgcggtc cgcatcgacc acatcggttc gaccgccgtg cccgggatgg acgccaagga cgtcatcgac gttcaggtga ccgttgcgtc acttgatgtc gccgacgaac tcgctgacgc gctgctgacg gccggctacc cggcggtacc ggggatcacc accgacacgc cgcacgatga tgatcctgcg ttgtggcaca agcgtttcca tgcctcggct gatccgggcc ggccgaccaa cgtgcatgtg agggtggagg gcaggccgaa ccagcggttc gcgctgatgt tcgtcgactg gctgcggtcc aatccgggcg cacaggccga ttacctggct gccaaacgca gggcgatggc gacccccgac tacgccgtgg ccaaagagcc gtggttcctc gatgcctacc gacgtgcgtg ggaatgggcc gacgcgaagg gatggcagcc ctag

Details for MyfoA.00139.a.B1.GE40748

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	43kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMV LRIGLTGGIG AGKSTVSATF SDLGGIIVDG DVIAREVVEP GTEGLAKLVE AFGDGILLPE GALNRPALAA VAFSDDEKRA TLNGIVHPLV AHRRSELIAA AHDDAVIIED IPLLVESQMA PMFPLVIIVN ADPEIRVKRL IEYRGFTEQD ARARIAAQAT EEQRRAVADV WLDNSGSPGA VVEQARALWH ERILPFAHNL KIRQPAHRVP VVVPYDPTWP DQARRIIARL NTACGHRAVR IDHIGSTAVP GMDAKDVIDV QVTVASLDVA DELADALLTA GYPAVPGITT DTPHDDDPAL WHKRFHASAD PGRPTNVHVR VEGRPNQRFA LMFVDWLRSN PGAQADYLAA KRRAMATPDY AVAKEPWFLD AYRRAWEW

Validated NT Sequence

ggcccattcc cacgcacgtc ggtaggcatc gaggaaccac ggctctttgg ccacggcgta gtcgggggtc gccatcgccc tgcgtttggc agccaggtaa tcggcctgtg cgcccggatt ggaccgcagc cagtcgacga acatcagcgc gaaccgctgg ttcggcctgc cctccaccct cacatgcacg ttggtcggcc ggcccggatc agccgaggca tggaaacgct tgtgccacaa cgcaggatca tcatcgtgcg gcgtgtcggt ggtgatcccc ggtaccgccg ggtagccggc cgtcagcagc gcgtcagcga gttcgtcggc gacatcaagt gacgcaacgg tcacctgaac gtcgatgacg tccttggcgt ccatcccggg cacggcggtc gaaccgatgt ggtcgatgcg gaccgcacgg tgtccgcagg ctgtattgag ccgcgcgatg atacggcgcg cctgatccgg ccaggtcggg tcatacggca ccacaacagg gacgcgatga gccggctgac ggatcttcag gttgtgcgcg aagggcagga tccgctcgtg ccagagcgcg cgagcctgtt cgacgacggc ccctgggctg cccgagttgt ccagccatac gtcggcgacc gcgcggcgct gctcctcggt ggcctgcgcg gcgatgcggg cgcgggcgtc ttgctcggtg aatccgcggt attcgatcag ccgcttcacg cgaatctccg ggtcggcgtt gacgatgatg accagcggga acatcggcgc catctgggat tcgaccaaca gtgggatgtc ctcgatgatc accgcatcgt cgtgcgcggc cgcgatcagc tcagaccggc ggtgcgccac cagcggatgg acgatgccgt tcagggtggc gcgcttctcg tcgtcgctga acgcgaccgc ggccagggcc gggcggttca gcgccccttc gggtagcagg atcccgtcac cgaacgcctc gacgagcttg gccagacctt cggttcccgg ttcgaccacc tcacgcgcga tgacgtcgcc gtcgacgatg atgccgccga gatcgctgaa tgtggccgac actgttgact tcccggcgcc gataccgccg gttaacccga tgcgaagcac catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHML RIGLTGGIGA GKSTVSATFS DLGGIIVDGD VIAREVVEPG TEGLAKLVEA FGDGILLPEG ALNRPALAAV AFSDDEKRAT LNGIVHPLVA HRRSELIAAA HDDAVIIEDI PLLVESQMAP MFPLVIIVNA DPEIRVKRLI EYRGFTEQDA RARIAAQATE EQRRAVADVW LDNSGSPGAV VEQARALWHE RILPFAHNLK IRQPAHRVPV VVPYDPTWPD QARRIIARLN TACGHRAVRI DHIGSTAVPG MDAKDVIDVQ VTVASLDVAD ELADALLTAG YPAVPGITTD TPHDDDPALW HKRFHASADP GRPTNVHVRV EGRPNQRFAL MFVDWLRSNP GAQADYLAAK RRAMATPDYA VAKEPWFLDA YRRAWEWADA KGWQP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcttc gcatcgggtt aaccggcggt atcggcgccg ggaagtcaac agtgtcggcc acattcagcg atctcggcgg catcatcgtc gacggcgacg tcatcgcgcg tgaggtggtc gaaccgggaa ccgaaggtct ggccaagctc gtcgaggcgt tcggtgacgg gatcctgcta cccgaagggg cgctgaaccg cccggccctg gccgcggtcg cgttcagcga cgacgagaag cgcgccaccc tgaacggcat cgtccatccg ctggtggcgc accgccggtc tgagctgatc gcggccgcgc acgacgatgc ggtgatcatc gaggacatcc cactgttggt cgaatcccag atggcgccga tgttcccgct ggtcatcatc gtcaacgccg acccggagat tcgcgtgaag cggctgatcg aataccgcgg attcaccgag caagacgccc gcgcccgcat cgccgcgcag gccaccgagg agcagcgccg cgcggtcgcc gacgtatggc tggacaactc gggcagccca ggggccgtcg tcgaacaggc tcgcgcgctc tggcacgagc ggatcctgcc cttcgcgcac aacctgaaga tccgtcagcc ggctcatcgc gtccctgttg tggtgccgta tgacccgacc tggccggatc aggcgcgccg tatcatcgcg cggctcaata cagcctgcgg acaccgtgcg gtccgcatcg accacatcgg ttcgaccgcc gtgcccggga tggacgccaa ggacgtcatc gacgttcagg tgaccgttgc gtcacttgat gtcgccgacg aactcgctga cgcgctgctg acggccggct acccggcggt accggggatc accaccgaca cgccgcacga tgatgatcct gcgttgtggc acaagcgttt ccatgcctcg gctgatccgg gccggccgac caacgtgcat gtgagggtgg agggcaggcc gaaccagcgg ttcgcgctga tgttcgtcga ctggctgcgg tccaatccgg gcgcacaggc cgattacctg gctgccaaac gcagggcgat ggcgaccccc gactacgccg tggccaaaga gccgtggttc ctcgatgcct accgacgtgc gtgggaatgg gccgacgcga agggatggca gccctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for MyfoA.00139.a.B2.GE40749

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	23kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

atcttactca cttaacggat cttcaggttg tgcgcgaagg gcaggatccg ctcgtgccag agcgcgcgag cctgttcgac gacggcccct gggctgcccg agttgtccag ccatacgtcg gcgaccgcgc ggcgctgctc ctcggtggcc tgcgcggcga tgcgggcgcg ggcgtcttgc tcggtgaatc cgcggtattc gatcagccgc ttcacgcgaa tctccgggtc ggcgttgacg atgatgacca gcgggaacat cggcgccatc tgggattcga ccaacagtgg gatgtcctcg atgatcaccg catcgtcgtg cgcggccgcg atcagctcag accggcggtg cgccaccagc ggatggacga tgccgttcag ggtggcgcgc ttctcgtcgt cgctgaacgc gaccgcggcc agggccgggc ggttcagcgc cccttcgggt agcaggatcc cgtcaccgaa cgcctcgacg agcttggcca gaccttcggt tcccggttcg accacctcac gcgcgatgac gtcgccgtcg acgatgatgc cgccgagatc gctgaatgtg gccgacactg ttgacttccc ggcgccgata ccgccggtta acccgatgcg aagcaccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcttc gcatcgggtt aaccggcggt atcggcgccg ggaagtcaac agtgtcggcc acattcagcg atctcggcgg catcatcgtc gacggcgacg tcatcgcgcg tgaggtggtc gaaccgggaa ccgaaggtct ggccaagctc gtcgaggcgt tcggtgacgg gatcctgcta cccgaagggg cgctgaaccg cccggccctg gccgcggtcg cgttcagcga cgacgagaag cgcgccaccc tgaacggcat cgtccatccg ctggtggcgc accgccggtc tgagctgatc gcggccgcgc acgacgatgc ggtgatcatc gaggacatcc cactgttggt cgaatcccag atggcgccga tgttcccgct ggtcatcatc gtcaacgccg acccggagat tcgcgtgaag cggctgatcg aataccgcgg attcaccgag caagacgccc gcgcccgcat cgccgcgcag gccaccgagg agcagcgccg cgcggtcgcc gacgtatggc tggacaactc gggcagccca ggggccgtcg tcgaacaggc tcgcgcgctc tggcacgagc ggatcctgcc cttcgcgcac aacctgaaga tccgttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MyfoA.00139.a.B1.PW38007

PURIFICATION DATe:	1/4/2017
CONCENTRATION:	33mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCTTC GCATCGGGTT AACCGGCGGT ATCGGCGCCG GGAAGTCAAC AGTGTCGGCC ACATTCAGCG ATCTCGGCGG CATCATCGTC GACGGCGACG TCATCGCGCG TGAGGTGGTC GAACCGGGAA CCGAAGGTCT GGCCAAGCTC GTCGAGGCGT TCGGTGACGG GATCCTGCTA CCCGAAGGGG CGCTGAACCG CCCGGCCCTG GCCGCGGTCG CGTTCAGCGA CGACGAGAAG CGCGCCACCC TGAACGGCAT CGTCCATCCG CTGGTGGCGC ACCGCCGGTC TGAGCTGATC GCGGCCGCGC ACGACGATGC GGTGATCATC GAGGACATCC CACTGTTGGT CGAATCCCAG ATGGCGCCGA TGTTCCCGCT GGTCATCATC GTCAACGCCG ACCCGGAGAT TCGCGTGAAG CGGCTGATCG AATACCGCGG ATTCACCGAG CAAGACGCCC GCGCCCGCAT CGCCGCGCAG GCCACCGAGG AGCAGCGCCG CGCGGTCGCC GACGTATGGC TGGACAACTC GGGCAGCCCA GGGGCCGTCG TCGAACAGGC TCGCGCGCTC TGGCACGAGC GGATCCTGCC CTTCGCGCAC AACCTGAAGA TCCGTCAGCC GGCTCATCGC GTCCCTGTTG TGGTGCCGTA TGACCCGACC TGGCCGGATC AGGCGCGCCG TATCATCGCG CGGCTCAATA CAGCCTGCGG ACACCGTGCG GTCCGCATCG ACCACATCGG TTCGACCGCC GTGCCCGGGA TGGACGCCAA GGACGTCATC GACGTTCAGG TGACCGTTGC GTCACTTGAT GTCGCCGACG AACTCGCTGA CGCGCTGCTG ACGGCCGGCT ACCCGGCGGT ACCGGGGATC ACCACCGACA CGCCGCACGA TGATGATCCT GCGTTGTGGC ACAAGCGTTT CCATGCCTCG GCTGATCCGG GCCGGCCGAC CAACGTGCAT GTGAGGGTGG AGGGCAGGCC GAACCAGCGG TTCGCGCTGA TGTTCGTCGA CTGGCTGCGG TCCAATCCGG GCGCACAGGC CGATTACCTG GCTGCCAAAC GCAGGGCGAT GGCGACCCCC GACTACGCCG TGGCCAAAGA GCCGTGGTTC CTCGATGCCT ACCGACGTGC GTGGGAATGG GCCGACGCGA AGGGATGGCA GCCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT