MyboA.01613.b

PROBABLE ACYL-COA DEHYDROGENASE FADE30

CENTER ID: MyboA.01613.b
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.01613.b.A1.GE30575 Full length( MyboA.01613.b ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.3933
RefSeq: NP_857229.1
UniProt: A0A0H3P6B7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQDVEEFRAQ VRGWLADNLA GEFAALKGLG GPGREHEAFE ERRAWNQRLA AAGLTCLGWP EEHGGRGLST AHRVAFYEEY ARADAPDKVN HFGEELLGPT LIAFGTPQQQ RRFLPRIRDV TELWCQGYSE PGAGSDLASV ATTAELDGDQ WVINGQKVWT SLAHLSQWCF VLARTEKGSQ RHAGLSYLLV PLDQPGVQIR PIVQITGTAE FNEVFFDDAR TDADLVVGAP GDGWRVAMAT LTFERGVSTL GQQIVYAREL SNLVELARRT AAADDPLIRE RLTRAWTGLR AMRSYALATM EGPAVEQPGQ DNVSKLLWAN WHRNLGELAM DVIGKPGMTM PDGEFDEWQR LYLFTRADTI YGGSNEIQRN IIAERVLGLP REAKG
NT Sequence
ATGCAGGACG TCGAGGAGTT CCGGGCGCAG GTCCGCGGTT GGCTCGCCGA CAATCTGGCC GGCGAATTCG CGGCACTTAA GGGCCTCGGC GGGCCGGGGC GCGAGCACGA AGCGTTCGAG GAACGCCGGG CGTGGAATCA GCGCCTGGCC GCCGCGGGGC TGACCTGCCT GGGCTGGCCG GAAGAACACG GCGGGCGGGG GCTCTCGACC GCGCACCGGG TGGCGTTCTA CGAGGAATAC GCCCGCGCCG ACGCGCCGGA CAAGGTCAAC CACTTCGGCG AGGAACTGCT GGGTCCGACG CTGATCGCGT TCGGAACGCC GCAGCAGCAG CGGCGCTTCC TGCCGCGCAT CCGCGACGTC ACCGAGCTGT GGTGTCAGGG ATATTCGGAG CCCGGCGCTG GCAGCGACCT GGCCAGCGTT GCGACCACCG CAGAACTCGA CGGCGACCAG TGGGTGATAA ACGGCCAGAA GGTGTGGACG TCGCTGGCGC ACCTGTCGCA ATGGTGCTTT GTGCTCGCAC GCACCGAGAA GGGCTCGCAG CGCCACGCCG GCCTGTCGTA TCTGCTGGTG CCGCTGGACC AGCCCGGCGT GCAGATCCGG CCGATCGTGC AGATCACCGG CACCGCGGAG TTCAACGAGG TGTTCTTCGA CGACGCCCGC ACCGACGCCG ACCTGGTGGT CGGCGCACCG GGCGACGGCT GGCGGGTCGC GATGGCGACG CTGACCTTCG AGCGCGGCGT CTCGACGCTG GGTCAGCAGA TCGTCTACGC CCGTGAGCTT TCCAATCTGG TCGAGCTGGC GCGGCGCACC GCCGCCGCGG ATGACCCGCT GATCCGGGAG CGGCTGACCC GGGCGTGGAC CGGGCTGCGC GCCATGCGCT CCTATGCTTT GGCCACCATG GAAGGGCCAG CCGTTGAGCA GCCCGGCCAG GACAATGTGT CAAAGTTGTT GTGGGCCAAC TGGCATCGCA ACCTGGGTGA GCTGGCCATG GACGTGATCG GTAAACCCGG AATGACCATG CCCGACGGCG AGTTCGACGA GTGGCAGCGG CTATATCTAT TCACCCGCGC CGACACCATC TACGGAGGAT CCAACGAGAT CCAGCGCAAC ATCATCGCCG AGCGGGTGCT CGGTCTGCCC CGGGAGGCCA AGGGA
Details for MyboA.01613.b.A1.GE30575
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQDVEEFRA QVRGWLADNL AGEFAALKGL GGPGREHEAF EERRAWNQRL AAAGLTCLGW PEEHGGRGLS TAHRVAFYEE YARADAPDKV NHFGEELLGP TLIAFGTPQQ QRRFLPRIRD VTELWCQGYS EPGAGSDLAS VATTAELDGD QWVINGQKVW TSLAHLSQWC FVLARTEKGS QRHAGLSYLL VPLDQPGVQI RPIVQITGTA XFNEVFFDDA RTDADLVVGA PGDGWRVAMA TLTFERGVST LGQQIVYARE LSNLVELARR TAAADDPLIR XRLTRAWXGX RAXRXYAXXT XGXXXVEXPX PGXXXXXVXG XXXXXXXXXX XXIXXPXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
ttttgtttac tttnagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcagga cgtcgaggag ttccgggcgc aggtccgcgg ttggctcgcc gacaatctgg ccggcgaatt cgcggcactt aagggcctcg gcgggccggg gcgcgagcac gaagcgttcg aggaacgccg ggcgtggaat cagcgcctgg ccgccgcggg gctgacctgc ctgggctggc cggaagaaca cggcgggcgg gggctctcga ccgcgcaccg ggtggcgttc tacgaggaat acgcccgcgc cgacgcgccg gacaaggtca accacttcgg cgaggaactg ctgggtccga cgctgatcgc gttcggaacg ccgcagcagc agcggcgctt cctgccgcgc atccgcgacg tcaccgagct gtggtgtcag ggatattcgg agcccggcgc tggcagcgac ctggccagcg ttgcgaccac cgcagaactc gacggcgacc agtgggtgat aaacggccag aaggtgtgga cgtcgctggc gcacctgtcg caatggtgct ttgtgctcgc acgcaccgag aagggctcgc agcgccacgc cggcctgtcg tatctgctgg tgccgctgga ccagcccggc gtgcagatcc ggccgatcgt gcagatcacc ggcaccgcgg anttcaacga ggtgttcttc gacgacgccc gcaccgacgc cgacctggtg gtcggcgcac cgggcgacgg ctggcgggtc gcgatggcga cgctgacctt cgagcgcggc gtctcgacgc tgggtcagca gatcgtctac gcccgtgagc tttccaatct ggtcgagctg gcgcggcgca ccgccgccgc ggatgacccg ctgatccggg ancggctgac ccgggcgtgg nccgggntgc gcgccntgcg ctnctatgct ttnnncacca ngggannnnn nnncgttgag cancccngnc caggncnatg nnnnnaantn gtnnnngggn nnncnngnnt cncnnnnnnn anctnnnntg nnnnnatcnn naanccnnnn nannnnnncn nnngnnnant nnnnnnannn nngnnnnntn tanntnnnnn nnnnnnnnnn nnngnnnncn nnnnnnnnnn c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQDVEEFRA QVRGWLADNL AGEFAALKGL GGPGREHEAF EERRAWNQRL AAAGLTCLGW PEEHGGRGLS TAHRVAFYEE YARADAPDKV NHFGEELLGP TLIAFGTPQQ QRRFLPRIRD VTELWCQGYS EPGAGSDLAS VATTAELDGD QWVINGQKVW TSLAHLSQWC FVLARTEKGS QRHAGLSYLL VPLDQPGVQI RPIVQITGTA EFNEVFFDDA RTDADLVVGA PGDGWRVAMA TLTFERGVST LGQQIVYARE LSNLVELARR TAAADDPLIR ERLTRAWTGL RAMRSYALAT MEGPAVEQPG QDNVSKLLWA NWHRNLGELA MDVIGKPGMT MPDGEFDEWQ RLYLFTRADT IYGGSNEIQR NIIAERVLGL PREAKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggacgtc gaggagttcc gggcgcaggt ccgcggttgg ctcgccgaca atctggccgg cgaattcgcg gcacttaagg gcctcggcgg gccggggcgc gagcacgaag cgttcgagga acgccgggcg tggaatcagc gcctggccgc cgcggggctg acctgcctgg gctggccgga agaacacggc gggcgggggc tctcgaccgc gcaccgggtg gcgttctacg aggaatacgc ccgcgccgac gcgccggaca aggtcaacca cttcggcgag gaactgctgg gtccgacgct gatcgcgttc ggaacgccgc agcagcagcg gcgcttcctg ccgcgcatcc gcgacgtcac cgagctgtgg tgtcagggat attcggagcc cggcgctggc agcgacctgg ccagcgttgc gaccaccgca gaactcgacg gcgaccagtg ggtgataaac ggccagaagg tgtggacgtc gctggcgcac ctgtcgcaat ggtgctttgt gctcgcacgc accgagaagg gctcgcagcg ccacgccggc ctgtcgtatc tgctggtgcc gctggaccag cccggcgtgc agatccggcc gatcgtgcag atcaccggca ccgcggagtt caacgaggtg ttcttcgacg acgcccgcac cgacgccgac ctggtggtcg gcgcaccggg cgacggctgg cgggtcgcga tggcgacgct gaccttcgag cgcggcgtct cgacgctggg tcagcagatc gtctacgccc gtgagctttc caatctggtc gagctggcgc ggcgcaccgc cgccgcggat gacccgctga tccgggagcg gctgacccgg gcgtggaccg ggctgcgcgc catgcgctcc tatgctttgg ccaccatgga agggccagcc gttgagcagc ccggccagga caatgtgtca aagttgttgt gggccaactg gcatcgcaac ctgggtgagc tggccatgga cgtgatcggt aaacccggaa tgaccatgcc cgacggcgag ttcgacgagt ggcagcggct atatctattc acccgcgccg acaccatcta cggaggatcc aacgagatcc agcgcaacat catcgccgag cgggtgctcg gtctgccccg ggaggccaag ggataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa