MyboA.01613.a

PROBABLE ACYL-COA DEHYDROGENASE FADE31

CENTER ID: MyboA.01613.a
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.01613.a.A1.GE30574 Full length( MyboA.01613.a ) 1 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.3935
RefSeq: NP_857231.1
UniProt: A0A0H3P666

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLNFDDETL AFQAEVREFL AANAASIPTK SYDNAEGFAQ HRYWDRVLFD AGLSVITWPA KYGGRDAPLL HWIVFEEEYF RAGAPGRASA NGTSMLAPTL FAHGTAEQLD RILPKMASGE QIWAQAWSEP ESGSDLASLR STASKVDGGW LLNGQKIWSS RAPFADMGFG LFRSDPAVER HRGLTYFMFD LKAKGVTVRP IAQLGGDTGF GEIFLDDVFV PDRDVIGAPN DGWRAAMSTS SNERGMSLRS PARFLASAER LVQLWKDRGS PPEFADRVAD AWIKAQAYRL QTFGTVTRLA AGGELGAESS VTKVFWSELD VHLHQTALDL RGADGELAGP WTEGLLFALG GPIYAGTNEI QRNIIAERLL GLPREKT
NT Sequence
ATGGACCTGA ATTTCGACGA CGAGACCCTG GCCTTTCAGG CCGAGGTGCG CGAGTTCCTC GCCGCCAATG CCGCATCGAT CCCGACGAAG TCCTACGACA ATGCGGAAGG CTTTGCGCAA CACCGTTATT GGGACCGAGT ACTGTTCGAC GCGGGCCTGT CGGTGATCAC CTGGCCGGCT AAGTATGGTG GCCGGGACGC GCCGCTGCTG CACTGGATCG TGTTCGAGGA GGAGTACTTT CGCGCCGGCG CCCCGGGCCG GGCCAGCGCC AACGGCACCT CGATGCTGGC GCCGACGCTG TTCGCGCACG GCACAGCCGA ACAGCTTGAC CGGATCCTGC CGAAAATGGC TAGCGGCGAA CAGATCTGGG CGCAGGCCTG GTCGGAGCCG GAATCCGGCA GCGACCTGGC GTCGCTGCGC TCCACCGCGA GCAAGGTCGA CGGCGGCTGG CTACTCAACG GGCAGAAGAT CTGGAGCTCG CGGGCGCCGT TCGCCGACAT GGGTTTTGGG CTGTTCCGCT CCGATCCCGC GGTCGAACGG CACCGCGGGC TCACGTATTT CATGTTCGAC CTGAAAGCCA AGGGTGTTAC CGTGCGCCCA ATCGCCCAAC TGGGCGGCGA CACCGGTTTC GGTGAGATCT TTCTCGACGA CGTGTTCGTC CCCGACCGGG ATGTGATTGG GGCACCGAAC GACGGATGGC GCGCGGCCAT GAGCACGTCA AGCAACGAGC GCGGCATGTC GCTGCGCAGC CCAGCCCGCT TCCTGGCCTC CGCCGAACGG CTGGTCCAGC TGTGGAAGGA CCGCGGCTCG CCCCCGGAGT TCGCCGACCG GGTCGCCGAC GCCTGGATCA AGGCGCAGGC CTACCGGCTG CAGACCTTCG GCACGGTGAC CAGGCTGGCC GCCGGTGGCG AACTGGGGGC GGAATCGTCG GTGACCAAGG TGTTCTGGTC CGAGCTGGAC GTGCACTTGC ATCAGACCGC GCTCGACCTG CGCGGCGCCG ATGGGGAGCT GGCCGGCCCG TGGACCGAGG GGTTGCTGTT CGCCCTGGGC GGCCCGATCT ATGCCGGGAC CAACGAAATC CAGCGCAACA TCATTGCCGA ACGGCTGCTG GGCCTGCCAC GCGAGAAGAC G
Details for MyboA.01613.a.A1.GE30574
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 72
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDLNFDDET LAFQAEVREF LAANAASIPT KSYDNAEGFA QHRYWDRVLF DAGLSVITWP AKYGGRDAPL LHWIVFEEEY FRAGAPGRAS ANGTSMLAPT LFAHGTAEQL DRILPKMASG EQIWAQAWSE PESGSDLASL RSTASKVDGG WLLNGXKIWS SRAPFADMGF GLFRSDPAVE RHRGLXYFMF XLKAXXXPCX QSXXWAXXXF RFXXXXXXXX GXXXXXXDXG XXXXXXQXXX XXXXXXXXXX XXXXXXXXXX XXXAXXXGXX XXXXXXXXXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacc tgaatttcga cgacgagacc ctggcctttc aggccgaggt gcgcgagttc ctcgccgcca atgccgcatc gatcccgacg aagtcctacg acaatgcgga aggctttgcg caacaccgtt attgggaccg agtactgttc gacgcgggcc tgtcggtgat cacctggccg gctaagtatg gtggccggga cgcgccgctg ctgcactgga tcgtgttcga ggaggagtac tttcgcgccg gcgccccggg ccgggccagc gccaacggca cctcgatgct ggcgccgacg ctgttcgcgc acggcacagc cgaacagctt gaccggatcc tgccgaaaat ggctagcggc gaacagatct gggcgcaggc ctggtcggag ccggaatccg gcagcgacct ggcgtcgctg cgctccaccg cgagcaaggt cgacggcggc tggctactca acgggcanaa gatctggagc tcgcgggcgc cgttcgccga catgggtttt gggctgttcc gctccgatcc cgcggtcgaa cggcaccgcg ggctcncgta tttcatgttc nacctgaaag ccannnngnt accgtgcncc caatcnnccn actgggcgng annncnnttt cgntttnnnn acnanntntn nncccnancn ggnngnnntn gggnnnccga ngacgnnggn nnnnnnnnnt nnnnnnntca gnannnnnnn nntgnnnnnt nnnnnnnnnn nnnnngncnn nnnnnnnann nnnnnnnnnt nnnangnnnn ncnnnnnnng cnnnnncnnt nggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLNFDDET LAFQAEVREF LAANAASIPT KSYDNAEGFA QHRYWDRVLF DAGLSVITWP AKYGGRDAPL LHWIVFEEEY FRAGAPGRAS ANGTSMLAPT LFAHGTAEQL DRILPKMASG EQIWAQAWSE PESGSDLASL RSTASKVDGG WLLNGQKIWS SRAPFADMGF GLFRSDPAVE RHRGLTYFMF DLKAKGVTVR PIAQLGGDTG FGEIFLDDVF VPDRDVIGAP NDGWRAAMST SSNERGMSLR SPARFLASAE RLVQLWKDRG SPPEFADRVA DAWIKAQAYR LQTFGTVTRL AAGGELGAES SVTKVFWSEL DVHLHQTALD LRGADGELAG PWTEGLLFAL GGPIYAGTNE IQRNIIAERL LGLPREKT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctgaat ttcgacgacg agaccctggc ctttcaggcc gaggtgcgcg agttcctcgc cgccaatgcc gcatcgatcc cgacgaagtc ctacgacaat gcggaaggct ttgcgcaaca ccgttattgg gaccgagtac tgttcgacgc gggcctgtcg gtgatcacct ggccggctaa gtatggtggc cgggacgcgc cgctgctgca ctggatcgtg ttcgaggagg agtactttcg cgccggcgcc ccgggccggg ccagcgccaa cggcacctcg atgctggcgc cgacgctgtt cgcgcacggc acagccgaac agcttgaccg gatcctgccg aaaatggcta gcggcgaaca gatctgggcg caggcctggt cggagccgga atccggcagc gacctggcgt cgctgcgctc caccgcgagc aaggtcgacg gcggctggct actcaacggg cagaagatct ggagctcgcg ggcgccgttc gccgacatgg gttttgggct gttccgctcc gatcccgcgg tcgaacggca ccgcgggctc acgtatttca tgttcgacct gaaagccaag ggtgttaccg tgcgcccaat cgcccaactg ggcggcgaca ccggtttcgg tgagatcttt ctcgacgacg tgttcgtccc cgaccgggat gtgattgggg caccgaacga cggatggcgc gcggccatga gcacgtcaag caacgagcgc ggcatgtcgc tgcgcagccc agcccgcttc ctggcctccg ccgaacggct ggtccagctg tggaaggacc gcggctcgcc cccggagttc gccgaccggg tcgccgacgc ctggatcaag gcgcaggcct accggctgca gaccttcggc acggtgacca ggctggccgc cggtggcgaa ctgggggcgg aatcgtcggt gaccaaggtg ttctggtccg agctggacgt gcacttgcat cagaccgcgc tcgacctgcg cggcgccgat ggggagctgg ccggcccgtg gaccgagggg ttgctgttcg ccctgggcgg cccgatctat gccgggacca acgaaatcca gcgcaacatc attgccgaac ggctgctggg cctgccacgc gagaagacgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa