SSGCID: Target Page

MyboA.01189.a

PUTATIVE QUINONE OXIDOREDUCTASE (NADPH:QUINONE OXIDOREDUCTASE) (ZETA-CRYSTALLIN)

CENTER ID:	MyboA.01189.a
ORGANISM:	Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyboA.01189.a.A1.GE30570	Full length( MyboA.01189.a )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|233413.5.peg.175
RefSeq:	NP_853820.1
UniProt:	A0A0H3P3Q0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKACVVKELS GPSGMVYTDI DEVSGDGGKV VIDVRAAGVC FPDLLLTKGE YQLKLTPPFV PGMETAGVVR SAPSDAGFHV GERVSAFGVL GGYAEQIAVP VANVVRSPVE LDDAGAVSLL VNYNTMYFAL ARRAALRPGD TVLVLGAAGG VGTAAVQIAK AMQAGKVIAM VHREGAIDYV ASLGADVVLP LTEGWAQQVR DHTYGQGVDI VVDPIGGPTF DDALGVLAID GKLLLIGFAA GAVPTLKVNR LLVRNISVVG VGWGEYLNAV PGSAALFAWG LNQLVFLGLR PPPPQRYPLS EAQAALQSLD DGGVLGKVVL EP

NT Sequence

ATGAAGGCTT GTGTCGTAAA AGAACTTTCC GGCCCGTCCG GCATGGTGTA CACCGACATC GACGAGGTAT CCGGTGACGG CGGAAAGGTT GTTATCGACG TACGGGCCGC CGGCGTCTGC TTTCCGGACC TGCTGCTGAC CAAGGGCGAG TATCAACTGA AGCTAACGCC GCCGTTCGTG CCCGGCATGG AAACGGCGGG TGTGGTGCGT TCGGCGCCGT CGGATGCGGG TTTTCATGTG GGCGAACGTG TTTCAGCATT CGGAGTGCTC GGCGGCTACG CCGAACAAAT AGCCGTACCG GTGGCCAATG TGGTTCGCAG CCCCGTCGAG CTCGATGACG CCGGGGCGGT GTCGCTGTTG GTGAACTACA ACACCATGTA CTTCGCCCTG GCTCGGCGTG CCGCGCTGCG ACCGGGAGAC ACCGTGCTGG TGCTCGGCGC CGCCGGCGGA GTGGGCACGG CCGCCGTCCA GATCGCGAAG GCGATGCAGG CTGGCAAGGT GATAGCCATG GTGCACCGCG AAGGTGCGAT CGACTATGTC GCTTCGCTCG GTGCCGACGT GGTGCTTCCG CTGACCGAGG GCTGGGCTCA GCAGGTGCGT GACCACACCT ACGGTCAGGG GGTGGACATC GTCGTCGATC CCATCGGCGG ACCGACATTC GACGACGCGC TCGGCGTGCT GGCGATCGAC GGCAAGTTAT TGTTGATCGG CTTTGCCGCG GGTGCTGTAC CGACCCTCAA GGTCAACCGG CTGCTGGTGC GCAATATCAG CGTGGTGGGC GTCGGGTGGG GCGAGTATCT CAACGCGGTT CCCGGTTCGG CCGCCTTGTT CGCCTGGGGG CTAAACCAGC TGGTCTTTCT GGGGCTCAGA CCGCCTCCGC CGCAACGCTA TCCGTTGTCG GAAGCACAGG CCGCGTTGCA GAGTCTGGAC GACGGCGGTG TGCTCGGCAA GGTTGTGCTC GAGCCC

Details for MyboA.01189.a.A1.GE30570

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	107

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKACVVKEL SGPSGMVYTD IDEVSGDGGK VVIDVRAAGV CFPDLLLTKG EYQLKLTPPF VPGMETAGVV RSAPSDAGFH VGERVSAFGV LGGYAEQIAV PVANVVRSPV ELDDAGAVSL LVNYNTMYFA LARRAALRPG DTVLVLGAAG GVGTAAVQIA KAMQAGKVIA MVHREGAIDY VASLGADVVL PLTEGWAQQV RDHTYGQGVD IVVDPIGGPT FDDALGVLAI DGKLLLIGFA AGAVPTLKVN RLLVRNISVV GVGWGEYLNA VPGSXALFAW GLNQLVFLGL XPPPPXRYPX SXAXXXLXXX XRXRXLGXXV LXPXKQXXXX XXXLXXXXXA XXXXXXXXXX

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaagg cttgtgtcgt aaaagaactt tccggcccgt ccggcatggt gtacaccgac atcgacgagg tatccggtga cggcggaaag gttgttatcg acgtacgggc cgccggcgtc tgctttccgg acctgctgct gaccaagggc gagtatcaac tgaagctaac gccgccgttc gtgcccggca tggaaacggc gggtgtggtg cgttcggcgc cgtcggatgc gggttttcat gtgggcgaac gtgtttcagc attcggagtg ctcggcggct acgccgaaca aatagccgta ccggtggcca atgtggttcg cagccccgtc gagctcgatg acgccggggc ggtgtcgctg ttggtgaact acaacaccat gtacttcgcc ctggctcggc gtgccgcgct gcgaccggga gacaccgtgc tggtgctcgg cgccgccggc ggagtgggca cggccgccgt ccagatcgcg aaggcgatgc aggctggcaa ggtgatagcc atggtgcacc gcgaaggtgc gatcgactat gtcgcttcgc tcggtgccga cgtggtgctt ccgctgaccg agggctgggc tcagcaggtg cgtgaccaca cctacggtca gggggtggac atcgtcgtcg atcccatcgg cggaccgaca ttcgacgacg cgctcggcgt gctggcgatc gacggcaagt tattgttgat cggctttgcc gcgggtgctg taccgaccct caaggtcaac cggctgctgg tgcgcaatat cagcgtggtg ggcgtcgggt ggggcgagta tctcaacgcg gttcccggtt cgnncgcctt gttcgcctgg gggctaaacc agctggtctt tctggggctc nnaccgcctc cgccgcancg ctatccgnng tcnnaagcac annccncntt gcnnantcnn gnacgannnc ggnggctcgg cnnnntngtg ctnnanccct anaaacagca nnaannannn cngcannnnc tnnncnnnnn nnnnncngcn nnnnanncnn nnngnancnn nntncnncnn nc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKACVVKEL SGPSGMVYTD IDEVSGDGGK VVIDVRAAGV CFPDLLLTKG EYQLKLTPPF VPGMETAGVV RSAPSDAGFH VGERVSAFGV LGGYAEQIAV PVANVVRSPV ELDDAGAVSL LVNYNTMYFA LARRAALRPG DTVLVLGAAG GVGTAAVQIA KAMQAGKVIA MVHREGAIDY VASLGADVVL PLTEGWAQQV RDHTYGQGVD IVVDPIGGPT FDDALGVLAI DGKLLLIGFA AGAVPTLKVN RLLVRNISVV GVGWGEYLNA VPGSAALFAW GLNQLVFLGL RPPPPQRYPL SEAQAALQSL DDGGVLGKVV LEP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggcttgt gtcgtaaaag aactttccgg cccgtccggc atggtgtaca ccgacatcga cgaggtatcc ggtgacggcg gaaaggttgt tatcgacgta cgggccgccg gcgtctgctt tccggacctg ctgctgacca agggcgagta tcaactgaag ctaacgccgc cgttcgtgcc cggcatggaa acggcgggtg tggtgcgttc ggcgccgtcg gatgcgggtt ttcatgtggg cgaacgtgtt tcagcattcg gagtgctcgg cggctacgcc gaacaaatag ccgtaccggt ggccaatgtg gttcgcagcc ccgtcgagct cgatgacgcc ggggcggtgt cgctgttggt gaactacaac accatgtact tcgccctggc tcggcgtgcc gcgctgcgac cgggagacac cgtgctggtg ctcggcgccg ccggcggagt gggcacggcc gccgtccaga tcgcgaaggc gatgcaggct ggcaaggtga tagccatggt gcaccgcgaa ggtgcgatcg actatgtcgc ttcgctcggt gccgacgtgg tgcttccgct gaccgagggc tgggctcagc aggtgcgtga ccacacctac ggtcaggggg tggacatcgt cgtcgatccc atcggcggac cgacattcga cgacgcgctc ggcgtgctgg cgatcgacgg caagttattg ttgatcggct ttgccgcggg tgctgtaccg accctcaagg tcaaccggct gctggtgcgc aatatcagcg tggtgggcgt cgggtggggc gagtatctca acgcggttcc cggttcggcc gccttgttcg cctgggggct aaaccagctg gtctttctgg ggctcagacc gcctccgccg caacgctatc cgttgtcgga agcacaggcc gcgttgcaga gtctggacga cggcggtgtg ctcggcaagg ttgtgctcga gccctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa