MyboA.01117.a

PROBABLE ACYL-COA DEHYDROGENASE FADE26

CENTER ID: MyboA.01117.a
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.01117.a.A1.GE30569 Full length( MyboA.01117.a ) 1 400
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.3873
RefSeq: NP_857173.1
UniProt: A0A0H3P676

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRISYTPQQE ELRRELRSYF ATLMTPERRE ALSSVQGEYG VGNVYRETIA QMGRDGWLAL GWPKEYGGQG RSAMDQLIFT DEAAIAGAPV PFLTINSVAP TIMAYGTDEQ KRFFLPRIAA GDLHFSIGYS EPGAGTDLAN LRTTAVRDGD DYVVNGQKMW TSLIQYADYV WLAVRTNPES SGAKKHRGIS VLIVPTTAEG FSWTPVHTMA GPDTSATYYS DVRVPVANRV GEENAGWKLV TNQLNHERVA LVSPAPIFGC LREVREWAQN TKDAGGTRLI DSEWVQLNLA RVHAKAEVLK LINWELASSQ SGPKDAGPSP ADASAAKVFG TELATEAYRL LMEVLGTAAT LRQNSPGALL RGRVERMHRA CLILTFGGGT NEVQRDIIGM VALGLPRANR
NT Sequence
ATGCGCATCA GTTACACCCC GCAGCAGGAG GAGCTGCGCC GCGAGCTGCG CTCGTACTTT GCCACGTTGA TGACGCCGGA ACGCCGGGAG GCGCTGAGCT CGGTCCAGGG TGAATACGGC GTCGGCAATG TCTACCGGGA GACGATCGCG CAAATGGGCC GCGACGGGTG GCTTGCGCTG GGCTGGCCCA AGGAATACGG CGGCCAGGGC CGCTCGGCGA TGGACCAGCT GATCTTCACC GATGAAGCCG CCATCGCCGG TGCACCGGTG CCGTTCCTGA CCATCAACAG CGTGGCGCCG ACGATCATGG CCTACGGAAC CGACGAGCAG AAGAGGTTTT TCCTGCCCCG GATCGCCGCC GGGGACCTGC ACTTCTCGAT CGGCTACTCC GAGCCCGGCG CCGGCACCGA CCTGGCCAAC CTGCGCACCA CCGCGGTTCG CGACGGCGAT GACTATGTGG TCAACGGCCA GAAGATGTGG ACCAGCCTGA TTCAGTACGC CGACTACGTC TGGTTAGCGG TACGCACCAA CCCGGAGTCT TCTGGGGCCA AAAAACACCG TGGCATATCG GTGTTAATCG TGCCGACGAC CGCTGAGGGC TTCTCCTGGA CTCCAGTGCA CACCATGGCC GGTCCGGACA CCAGCGCCAC CTACTACTCC GACGTGCGGG TACCGGTGGC CAACCGGGTC GGTGAGGAAA ACGCCGGCTG GAAGCTGGTG ACCAACCAGC TCAACCACGA GCGGGTCGCC CTGGTGTCGC CGGCACCGAT TTTCGGATGC CTGCGCGAGG TCCGCGAATG GGCACAAAAC ACCAAGGACG CCGGCGGCAC CAGGCTGATC GACTCGGAGT GGGTGCAGCT CAACCTGGCC CGGGTACACG CCAAGGCCGA AGTCCTCAAG CTGATCAACT GGGAGCTGGC TTCCTCGCAA AGTGGGCCGA AGGACGCTGG ACCGTCACCG GCCGATGCGT CGGCGGCCAA GGTGTTCGGT ACCGAGCTGG CCACCGAGGC CTACCGGCTG CTGATGGAGG TGTTGGGCAC TGCGGCGACC CTGCGCCAGA ATTCGCCAGG CGCGTTGCTG CGCGGCCGCG TCGAACGGAT GCACCGGGCG TGCCTGATCC TGACGTTCGG CGGCGGCACC AACGAAGTCC AGCGCGACAT CATCGGCATG GTCGCGCTGG GACTGCCGCG AGCCAACCGC
Details for MyboA.01117.a.A1.GE30569
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRISYTPQQ EELRRELRSY FATLMTPERR EALSSVQGEY GVGNVYRETI AQMGRDGWLA LGWPKEYGGQ GRSAMDQLIF TDEAAIAGAP VPFLTINSVA PTIMAYGTDE QKRFFLPRIA AGDLHFSIGY SEPGAGTDLA NLRTTAVRDG DDYVVNGQKM WTSLIQYADY VWLAVRTNPE SSGAKKHRGI SVLIVPTTAX GFSWTPVHTM AGPDTSATYY SDVRVPVANR VGEENAGWKL VTXQLNHERV ALGVXXHXFS DACARSAXWA QNTKDAXGTX XXDSXWXQLN LAXXXXXXEV LXLXXXGXGX XRXGXKXAXX XXXXPXXXXX XXXXXXXXXX XXXXXXXXXX XXXXPXXXXX XXXXXXPXXX XXXXXXXXXX XXX
Validated NT Sequence
attttgttnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgca tcagttacac cccgcagcag gaggagctgc gccgcgagct gcgctcgtac tttgccacgt tgatgacgcc ggaacgccgg gaggcgctga gctcggtcca gggtgaatac ggcgtcggca atgtctaccg ggagacgatc gcgcaaatgg gccgcgacgg gtggcttgcg ctgggctggc ccaaggaata cggcggccag ggccgctcgg cgatggacca gctgatcttc accgatgaag ccgccatcgc cggtgcaccg gtgccgttcc tgaccatcaa cagcgtggcg ccgacgatca tggcctacgg aaccgacgag cagaagaggt ttttcctgcc ccggatcgcc gccggggacc tgcacttctc gatcggctac tccgagcccg gcgccggcac cgacctggcc aacctgcgca ccaccgcggt tcgcgacggc gatgactatg tggtcaacgg ccagaagatg tggaccagcc tgattcagta cgccgactac gtctggttag cggtacgcac caacccggag tcttctgggg ccaaaaaaca ccgtggcata tcggtgttaa tcgtgccgac gaccgctgan ggcttctcct ggactccagt gcacaccatg gccggtccgg acaccagcgc cacctactac tccgacgtgc gggtaccggt ggccaaccgg gtcggtgagg aaaacgccgg ctggaagctg gtgaccnncc agctcaacca cgagcgggtc gccctgggng tcgncnggca ccnattttcg gatgcctgcg cgaggtccgc gaantgggca caaaacacca aggacgccgn cggcaccang cnnatngact cnnantgggn gcagctcaac ctggcccngg nacncnccnn nnncgaagtc ctnnagctga tnnacnnggg anctggnntn nntcgnnngg gncnnaagna cgcnnngnnn ncnntcncnn gnccnanncn nnngnngncn annnngttnn nnancgannn nnnnnnnncn annncntnnn ngantnnnnn nnnnngggnn nnntnnnnnn nnccnnnnnc cnnnantnnn nnnngnnnnn nnncntnncc nnnnnnnnnn cnnnnnnnnn nnnnnnggnn nnnnnnnnnn cnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRISYTPQQ EELRRELRSY FATLMTPERR EALSSVQGEY GVGNVYRETI AQMGRDGWLA LGWPKEYGGQ GRSAMDQLIF TDEAAIAGAP VPFLTINSVA PTIMAYGTDE QKRFFLPRIA AGDLHFSIGY SEPGAGTDLA NLRTTAVRDG DDYVVNGQKM WTSLIQYADY VWLAVRTNPE SSGAKKHRGI SVLIVPTTAE GFSWTPVHTM AGPDTSATYY SDVRVPVANR VGEENAGWKL VTNQLNHERV ALVSPAPIFG CLREVREWAQ NTKDAGGTRL IDSEWVQLNL ARVHAKAEVL KLINWELASS QSGPKDAGPS PADASAAKVF GTELATEAYR LLMEVLGTAA TLRQNSPGAL LRGRVERMHR ACLILTFGGG TNEVQRDIIG MVALGLPRAN R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcatcagt tacaccccgc agcaggagga gctgcgccgc gagctgcgct cgtactttgc cacgttgatg acgccggaac gccgggaggc gctgagctcg gtccagggtg aatacggcgt cggcaatgtc taccgggaga cgatcgcgca aatgggccgc gacgggtggc ttgcgctggg ctggcccaag gaatacggcg gccagggccg ctcggcgatg gaccagctga tcttcaccga tgaagccgcc atcgccggtg caccggtgcc gttcctgacc atcaacagcg tggcgccgac gatcatggcc tacggaaccg acgagcagaa gaggtttttc ctgccccgga tcgccgccgg ggacctgcac ttctcgatcg gctactccga gcccggcgcc ggcaccgacc tggccaacct gcgcaccacc gcggttcgcg acggcgatga ctatgtggtc aacggccaga agatgtggac cagcctgatt cagtacgccg actacgtctg gttagcggta cgcaccaacc cggagtcttc tggggccaaa aaacaccgtg gcatatcggt gttaatcgtg ccgacgaccg ctgagggctt ctcctggact ccagtgcaca ccatggccgg tccggacacc agcgccacct actactccga cgtgcgggta ccggtggcca accgggtcgg tgaggaaaac gccggctgga agctggtgac caaccagctc aaccacgagc gggtcgccct ggtgtcgccg gcaccgattt tcggatgcct gcgcgaggtc cgcgaatggg cacaaaacac caaggacgcc ggcggcacca ggctgatcga ctcggagtgg gtgcagctca acctggcccg ggtacacgcc aaggccgaag tcctcaagct gatcaactgg gagctggctt cctcgcaaag tgggccgaag gacgctggac cgtcaccggc cgatgcgtcg gcggccaagg tgttcggtac cgagctggcc accgaggcct accggctgct gatggaggtg ttgggcactg cggcgaccct gcgccagaat tcgccaggcg cgttgctgcg cggccgcgtc gaacggatgc accgggcgtg cctgatcctg acgttcggcg gcggcaccaa cgaagtccag cgcgacatca tcggcatggt cgcgctggga ctgccgcgag ccaaccgcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a