MyboA.01048.a

PROBABLE ACYL-COA DEHYDROGENASE FADE8

CENTER ID: MyboA.01048.a
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.01048.a.A1.GE30568 Full length( MyboA.01048.a ) 1 542
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.753
RefSeq: NP_854349.1
UniProt: A0A0H3P212

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTHVVTNQ VPPLENYNPA SSPVLIEALI QEGGQWGLDE VNEVGAISAS CQAQRWGELA DRNRPILHTH DAYGYRVDEV EYDPAYHELM RTAITHGMHA APWADDRPGA HVVRAAKTSV WTVEPGHICP ISMTYAVVPA LRYNSELAAV YEPLLTSREY DPELKPATTK AGITAGMSMT EKQGGSDVRA GTTQATPNAD GSYSLTGHKW FTSAPMCDIF LVLAQAPDGL SCFLLPRVLP DGTRNRMFLQ RLKDKLGNHA NASSEVEYDG AVAWLVGEEG RGVPTIIEMV NLTRLDCALG SATSMRTGLT RAVHHAQHRK AFGAYLIDQP LMRNVLADLA VEAEAATIVA MRMAGATDNA VRGNETEALL RRIGLAAAKY WVCKRSTAHA AEALECLGGN GYVEDSGMPR LYREAPLMGI WEGSGNVSAL DTLRAMATRP ACVEVLFDEL ARSAGQDPRL DGHVERLRPQ LGDLDTIGYR ARKIAEDICL ALQGSLLVRH GHPAVAEAFL ATRLGGQWGG AYGTMPAGLD LAPILERALV KG
NT Sequence
ATGTCAGATA CGCATGTCGT CACCAACCAG GTTCCGCCCT TGGAGAACTA CAATCCCGCG TCATCCCCGG TGCTCATCGA GGCTCTGATC CAGGAGGGTG GCCAGTGGGG CCTGGATGAA GTAAACGAGG TCGGGGCAAT TTCTGCCAGC TGCCAAGCCC AACGCTGGGG AGAGCTTGCA GACCGCAACC GGCCCATCCT GCATACCCAC GACGCTTACG GGTACCGGGT CGATGAGGTG GAGTACGACC CGGCCTACCA CGAGCTGATG CGTACCGCGA TCACCCATGG CATGCACGCC GCACCGTGGG CTGACGACCG CCCGGGTGCG CACGTGGTGC GAGCGGCCAA GACATCGGTG TGGACCGTCG AGCCGGGCCA TATCTGCCCC ATCTCGATGA CCTACGCCGT CGTTCCGGCG CTGCGGTATA ACTCCGAGCT GGCTGCGGTC TACGAGCCGC TGCTGACCAG TCGTGAGTAC GACCCGGAGC TGAAGCCGGC GACCACGAAG GCCGGCATCA CCGCCGGCAT GTCGATGACC GAGAAGCAGG GTGGCTCCGA CGTGCGCGCT GGCACCACCC AGGCGACCCC GAATGCGGAC GGCAGCTACA GCTTGACCGG CCACAAGTGG TTCACTTCGG CGCCGATGTG CGACATCTTC CTGGTGCTCG CGCAGGCACC GGACGGGCTG TCGTGCTTCC TGCTGCCGCG GGTGCTGCCC GACGGCACCC GCAACCGAAT GTTCTTGCAG CGGCTCAAGG ACAAGCTCGG CAACCACGCA AACGCCTCGA GCGAGGTCGA ATACGACGGT GCCGTCGCGT GGCTGGTGGG CGAGGAGGGC CGCGGCGTGC CGACCATCAT CGAGATGGTC AACCTCACCC GGCTGGACTG CGCTCTGGGC AGTGCCACCA GCATGCGCAC CGGCCTAACC CGCGCCGTCC ACCATGCCCA GCATCGGAAG GCGTTCGGCG CCTACCTGAT CGACCAGCCG TTGATGCGCA ACGTGCTGGC CGACCTGGCG GTGGAGGCCG AGGCCGCCAC CATCGTGGCA ATGCGGATGG CCGGTGCCAC CGACAACGCG GTGCGCGGGA ACGAGACCGA AGCGCTGCTG CGTCGCATCG GCCTGGCGGC CGCCAAGTAC TGGGTGTGCA AGCGCTCCAC CGCTCACGCC GCCGAAGCGC TGGAGTGCCT GGGCGGCAAC GGTTATGTCG AGGATTCCGG GATGCCCCGG CTCTACCGGG AGGCGCCGTT GATGGGCATC TGGGAGGGCT CGGGCAATGT CAGCGCGCTA GATACCTTGC GCGCCATGGC AACCCGGCCC GCATGCGTCG AGGTGCTGTT TGACGAGCTG GCCCGCAGCG CAGGCCAGGA CCCCAGGCTG GACGGCCACG TCGAAAGGCT GCGTCCGCAG CTAGGCGATC TTGACACGAT CGGTTATCGA GCCCGCAAGA TTGCCGAAGA CATCTGCCTG GCGTTGCAGG GATCGTTGTT GGTGCGCCAC GGACATCCCG CCGTCGCCGA GGCGTTTCTG GCCACTCGGC TCGGCGGCCA GTGGGGCGGA GCGTACGGCA CCATGCCGGC CGGTCTGGAT CTCGCGCCCA TCCTCGAGCG TGCGCTGGTA AAAGGC
Details for MyboA.01048.a.A1.GE30568
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 60kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 53
PERCENT COVERAGE: 30
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTHVVTN QVPPLENYNP AXXXVXXEAL IQEGGQWGXX XXNXVGXISX XCXXQRWGEL GYXXRPILXX XXXXXXXXDX VXYXXXXXXX XXTXXXXXCT PHRGLTTARV RTWCERPRHR CGPSSRAISA PSRWFRRCGI TPSWLRSTSR C
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcag atacgcatgt cgtcaccaac caggttccgc ccttggagaa ctacaatccc gcnnnnnccc nngtgnnann ngaggctctg atccaggagg gtggccagtg gggcnnggnn gangnnaacn aggtcggggn aatttctgnc nnctgccnnn nccaacgntg gggagagctt ggntaccnca nccggcccat cctgnntncn nncnacnctn ncnggnaccn ggncgatgan gtggantacn acnnngncta nnnngannng angnnnacng anatnncncn ngantgcacg ccgcaccgtg ggctgacgac cgcccgggtg cgcacgtggt gcgagcggcc aagacatcgg tgtggaccgt cgagccgggc catatctgcc ccatctcgat ggttccggcg ctgcggtata actccgagct ggctgcggtc tacgagccgc tgctgaccag tcgtgagtac gacccggagc tgaagccggc gaccacgaan gccggcatca ccnnnggnnt gtcnatgacc gagaagcang gtggctccga cntgcgcgct ggcaccaccc aggcgnnccc gaatgcnnac ggcagctacn ncttgaccgg ccacaagtgg ttcacttcng cgccnatgtg cgacatcttc nntggtgctc gcgcnnnacc gganggnctg tcntgcttcc tgctgccgcg ggtgctgccc nacngcnccc gcaaccgaat gttcnttgnn gcggntcnng gacnanctcn ncaaccacnn naacncctcn annnaggnnn natannanng t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTHVVTN QVPPLENYNP ASSPVLIEAL IQEGGQWGLD EVNEVGAISA SCQAQRWGEL ADRNRPILHT HDAYGYRVDE VEYDPAYHEL MRTAITHGMH AAPWADDRPG AHVVRAAKTS VWTVEPGHIC PISMTYAVVP ALRYNSELAA VYEPLLTSRE YDPELKPATT KAGITAGMSM TEKQGGSDVR AGTTQATPNA DGSYSLTGHK WFTSAPMCDI FLVLAQAPDG LSCFLLPRVL PDGTRNRMFL QRLKDKLGNH ANASSEVEYD GAVAWLVGEE GRGVPTIIEM VNLTRLDCAL GSATSMRTGL TRAVHHAQHR KAFGAYLIDQ PLMRNVLADL AVEAEAATIV AMRMAGATDN AVRGNETEAL LRRIGLAAAK YWVCKRSTAH AAEALECLGG NGYVEDSGMP RLYREAPLMG IWEGSGNVSA LDTLRAMATR PACVEVLFDE LARSAGQDPR LDGHVERLRP QLGDLDTIGY RARKIAEDIC LALQGSLLVR HGHPAVAEAF LATRLGGQWG GAYGTMPAGL DLAPILERAL VKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagatacg catgtcgtca ccaaccaggt tccgcccttg gagaactaca atcccgcgtc atccccggtg ctcatcgagg ctctgatcca ggagggtggc cagtggggcc tggatgaagt aaacgaggtc ggggcaattt ctgccagctg ccaagcccaa cgctggggag agcttgcaga ccgcaaccgg cccatcctgc atacccacga cgcttacggg taccgggtcg atgaggtgga gtacgacccg gcctaccacg agctgatgcg taccgcgatc acccatggca tgcacgccgc accgtgggct gacgaccgcc cgggtgcgca cgtggtgcga gcggccaaga catcggtgtg gaccgtcgag ccgggccata tctgccccat ctcgatgacc tacgccgtcg ttccggcgct gcggtataac tccgagctgg ctgcggtcta cgagccgctg ctgaccagtc gtgagtacga cccggagctg aagccggcga ccacgaaggc cggcatcacc gccggcatgt cgatgaccga gaagcagggt ggctccgacg tgcgcgctgg caccacccag gcgaccccga atgcggacgg cagctacagc ttgaccggcc acaagtggtt cacttcggcg ccgatgtgcg acatcttcct ggtgctcgcg caggcaccgg acgggctgtc gtgcttcctg ctgccgcggg tgctgcccga cggcacccgc aaccgaatgt tcttgcagcg gctcaaggac aagctcggca accacgcaaa cgcctcgagc gaggtcgaat acgacggtgc cgtcgcgtgg ctggtgggcg aggagggccg cggcgtgccg accatcatcg agatggtcaa cctcacccgg ctggactgcg ctctgggcag tgccaccagc atgcgcaccg gcctaacccg cgccgtccac catgcccagc atcggaaggc gttcggcgcc tacctgatcg accagccgtt gatgcgcaac gtgctggccg acctggcggt ggaggccgag gccgccacca tcgtggcaat gcggatggcc ggtgccaccg acaacgcggt gcgcgggaac gagaccgaag cgctgctgcg tcgcatcggc ctggcggccg ccaagtactg ggtgtgcaag cgctccaccg ctcacgccgc cgaagcgctg gagtgcctgg gcggcaacgg ttatgtcgag gattccggga tgccccggct ctaccgggag gcgccgttga tgggcatctg ggagggctcg ggcaatgtca gcgcgctaga taccttgcgc gccatggcaa cccggcccgc atgcgtcgag gtgctgtttg acgagctggc ccgcagcgca ggccaggacc ccaggctgga cggccacgtc gaaaggctgc gtccgcagct aggcgatctt gacacgatcg gttatcgagc ccgcaagatt gccgaagaca tctgcctggc gttgcaggga tcgttgttgg tgcgccacgg acatcccgcc gtcgccgagg cgtttctggc cactcggctc ggcggccagt ggggcggagc gtacggcacc atgccggccg gtctggatct cgcgcccatc ctcgagcgtg cgctggtaaa aggctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa