MyboA.00896.a
Putative citrate synthase 2
| CENTER ID: | MyboA.00896.a |
| ORGANISM: | Mycobacterium bovis AF2122/97 / ATCC BAA-935 |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyboA.00896.a.A1.GE30566 | Full length( MyboA.00896.a ) | 1 | 373 | ||
| MyboA.00896.a.AE1.GE43237 | Full length( MyboA.00896.a ) | 1 | 373 | ||
| MyboA.00896.a.AE1.GE43403 | Full length( MyboA.00896.a ) | 1 | 373 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyboA.00896.a.A1.PS00947 | Full length( MyboA.00896.a ) | 1 | 373 | ||
| MyboA.00896.a.AE1.PW38757 | Full length( MyboA.00896.a ) | 1 | 373 |
Structures
6WGY
DEPOSITED: 4/6/2020
DETERMINATION: XRay
CLONE: MyboA.00896.a.AE1.GE43403
PROTEIN: MyboA.00896.a.AE1.PS38615
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|233413.5.peg.994 |
| RefSeq: | NP_854570.1 |
| UniProt: | P63778 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVVPENFVP GLDGVVAFTT EIAEPDKDGG ALRYRGVDIE DLVSQRVTFG DVWALLVDGN FGSGLPPAEP FPLPIHSGDV RVDVQAGLAM LAPIWGYAPL LDIDDATARQ QLARASVMAL SYVAQSARGI YQPAVPQRII DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MLDEVERAGD ARSVVKGILD RGEKLMGFGH RVYRAEDPRA RVLRAAAERL GAPRYEVAVA VEQAALSELR ERRPDRAIET NVEFWAAVVL DFARVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPGPRSP ESVDGWERVL TTA
NT Sequence
ATGACAGTGG TCCCGGAGAA TTTCGTCCCC GGCCTCGACG GCGTGGTGGC CTTTACGACC GAGATCGCCG AGCCGGACAA AGACGGCGGG GCCCTGCGCT ACCGTGGCGT CGACATCGAA GACCTGGTAA GTCAGCGGGT CACCTTCGGC GATGTGTGGG CGCTGCTGGT GGACGGCAAC TTCGGCAGCG GGCTGCCGCC GGCTGAACCG TTCCCGCTGC CGATTCACTC CGGCGATGTG CGCGTCGACG TCCAGGCCGG CCTGGCGATG CTGGCGCCCA TCTGGGGATA TGCGCCGCTG CTCGACATCG ACGACGCCAC CGCCCGCCAA CAGCTGGCCC GGGCATCGGT GATGGCGCTG TCCTATGTCG CGCAATCCGC ACGGGGCATC TACCAGCCGG CGGTCCCGCA GCGAATCATC GATGAATGCT CGACGGTCAC AGCACGTTTC ATGACGCGCT GGCAGGGCGA GCCAGACCCC AGACACATCG AGGCCATTGA CGCCTACTGG GTGTCGGCCG CCGAGCACGG CATGAACGCT TCGACATTCA CCGCGCGTGT GATCGCCTCA ACTGGGGCGG ATGTGGCAGC AGCGCTGTCG GGCGCAATCG GGGCGATGAG CGGACCGCTG CATGGTGGCG CGCCGGCGCG CGTCCTGCCG ATGCTCGACG AGGTCGAACG CGCCGGCGAT GCCCGCAGCG TGGTCAAGGG AATCCTGGAC CGCGGCGAGA AACTGATGGG GTTCGGGCAC CGGGTCTACC GCGCCGAGGA CCCGCGGGCG CGGGTGCTGC GGGCGGCCGC CGAGCGGCTG GGCGCCCCCC GCTACGAGGT CGCGGTCGCG GTGGAGCAGG CTGCGCTGTC AGAACTGCGA GAGCGCCGTC CGGATCGGGC GATCGAGACC AATGTCGAAT TCTGGGCCGC AGTGGTCCTG GACTTTGCCC GGGTACCGGC CAACATGATG CCAGCAATGT TCACCTGTGG GCGCACTGCC GGCTGGTGTG CCCACATTCT CGAGCAGAAG CGACTCGGCA AGCTGGTCCG CCCGTCGGCC ATTTACGTGG GACCGGGCCC GCGCAGCCCG GAATCGGTCG ACGGCTGGGA GCGGGTACTC ACCACCGCC
Details for MyboA.00896.a.A1.GE30566
| HARVESTED ON: | 9/21/2010 |
| SEQUENCED ON: | 9/28/2010 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 80 |
Validated AA Sequence
MCGRCWWTAT SAAGCRRLNR SRCRFTPAMC ASTSRPALAM LAPIWGYAPL LDIDDATARQ QLARASVMAL SYVAQSARGI YQPAVPQRII DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MLDEVERAGD ARSVVKGILD RGEKLMGFGH RVYRAEDPRA RVLRAAAERL GAPRYEVAVA VEQAALSELR ERRPDRAIET NVEFWAAVVL DFARVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPGPRSP ESVDGWERV
Validated NT Sequence
agtacccgct cccagccgtc gaccgattcc gggctgcgcg ggcccggtcc cacgtaaatg gccgacgggc ggaccagctt gccgagtcgc ttctgctcga gaatgtgggc acaccagccg gcagtgcgcc cacaggtgaa cattgctggc atcatgttgg ccggtacccg ggcaaagtcc aggaccactg cggcccagaa ttcgacattg gtctcgatcg cccgatccgg acggcgctct cgcagttctg acagcgcagc ctgctccacc gcgaccgcga cctcgtagcg gggggcgccc agccgctcgg cggccgcccg cagcacccgc gcccgcgggt cctcggcgcg gtagacccgg tgcccgaacc ccatcagttt ctcgccgcgg tccaggattc ccttgaccac gctgcgggca tcgccggcgc gttcgacctc gtcgagcatc ggcaggacgc gcgccggcgc gccaccatgc agcggtccgc tcatcgcccc gattgcgccc gacagcgctg ctgccacatc cgccccagtt gaggcgatca cacgcgcggt gaatgtcgaa gcgttcatgc cgtgctcggc ggccgacacc cagtaggcgt caatggcctc gatgtgtctg gggtctggct cgccctgcca gcgcgtcatg aaacgtgctg tgaccgtcga gcattcatcg atgattcgct gcgggaccgc cggctggtag atgccccgtg cggattgcgc gacataggac agcgccatca ccgatgcccg ggccagctgt tggcgggcgg tggcgtcgtc gatgtcgagc agcggcgcat atccccagat gggcgccagc atcgccaggg ccggcctgga cgtcgacgcg cacatcgccg gagtgaatcg gcagcgggaa cggttcagcc ggcggcagcc cgctgccgaa gttgccgtcc accagcagcg cccacacatc gccgaaggtg acccgctgac ttaccaggtc ttngangtcg acgccacggt agcgcagggc cccgccgtct ttgtccggct cggcgatctc ggtcgtaaag gccaccacgc cgtcgaggcc ggggacgaaa ttctccggga ccactgtcat cgaaccagga ccctgggtct gagctnccag ggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVPENFV PGLDGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSQRVTF GDVWALLVDG NFGSGLPPAE PFPLPIHSGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR QQLARASVMA LSYVAQSARG IYQPAVPQRI IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMLDEVERAG DARSVVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRAAAER LGAPRYEVAV AVEQAALSEL RERRPDRAIE TNVEFWAAVV LDFARVPANM MPAMFTCGRT AGWCAHILEQ KRLGKLVRPS AIYVGPGPRS PESVDGWERV LTTA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagtggtc ccggagaatt tcgtccccgg cctcgacggc gtggtggcct ttacgaccga gatcgccgag ccggacaaag acggcggggc cctgcgctac cgtggcgtcg acatcgaaga cctggtaagt cagcgggtca ccttcggcga tgtgtgggcg ctgctggtgg acggcaactt cggcagcggg ctgccgccgg ctgaaccgtt cccgctgccg attcactccg gcgatgtgcg cgtcgacgtc caggccggcc tggcgatgct ggcgcccatc tggggatatg cgccgctgct cgacatcgac gacgccaccg cccgccaaca gctggcccgg gcatcggtga tggcgctgtc ctatgtcgcg caatccgcac ggggcatcta ccagccggcg gtcccgcagc gaatcatcga tgaatgctcg acggtcacag cacgtttcat gacgcgctgg cagggcgagc cagaccccag acacatcgag gccattgacg cctactgggt gtcggccgcc gagcacggca tgaacgcttc gacattcacc gcgcgtgtga tcgcctcaac tggggcggat gtggcagcag cgctgtcggg cgcaatcggg gcgatgagcg gaccgctgca tggtggcgcg ccggcgcgcg tcctgccgat gctcgacgag gtcgaacgcg ccggcgatgc ccgcagcgtg gtcaagggaa tcctggaccg cggcgagaaa ctgatggggt tcgggcaccg ggtctaccgc gccgaggacc cgcgggcgcg ggtgctgcgg gcggccgccg agcggctggg cgccccccgc tacgaggtcg cggtcgcggt ggagcaggct gcgctgtcag aactgcgaga gcgccgtccg gatcgggcga tcgagaccaa tgtcgaattc tgggccgcag tggtcctgga ctttgcccgg gtaccggcca acatgatgcc agcaatgttc acctgtgggc gcactgccgg ctggtgtgcc cacattctcg agcagaagcg actcggcaag ctggtccgcc cgtcggccat ttacgtggga ccgggcccgc gcagcccgga atcggtcgac ggctgggagc gggtactcac caccgcctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyboA.00896.a.AE1.GE43237
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MMTVVPENFV PGLDGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSQRVTF GDVWALLVDG NFGSGLPPAE PFPLPIHSGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR QQLARASVMA LSYVAQSARG IYQPAVPQRI IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMLDEVERAG DARSVVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRAAAER LGAPRYEVAV AVEQAALSEL RERRPDRAIE TNVEFWAAVV LDFARVPANM MPAMFTCGRT AGWCAHILEQ KRLGKLVRPS AIYVGPGPRS PESVDGWERV LTTAAGHHHH HH
Validated NT Sequence
tctctcccat gatgacagtg gtcccggaga atttcgtccc cggcctcgac ggcgtggtgg cctttacgac cgagatcgcc gagccggaca aagacggcgg ggccctgcgc taccgtggcg tcgacatcga agacctggta agtcagcggg tcaccttcgg cgatgtgtgg gcgctgctgg tggacggcaa cttcggcagc gggctgccgc cggctgaacc gttcccgctg ccgattcact ccggcgatgt gcgcgtcgac gtccaggccg gcctggcgat gctggcgccc atctggggat atgcgccgct gctcgacatc gacgacgcca ccgcccgcca acagctggcc cgggcatcgg tgatggcgct gtcctatgtc gcgcaatccg cacggggcat ctaccagccg gcggtcccgc agcgaatcat cgatgaatgc tcgacggtca cagcacgttt catgacgcgc tggcagggcg agccagaccc cagacacatc gaggccattg acgcctactg ggtgtcggcc gccgagcacg gcatgaacgc ttcgacattc accgcgcgtg tgatcgcctc aactggggcg gatgtggcag cagcgctgtc gggcgcaatc ggggcgatga gcggaccgct gcatggtggc gcgccggcgc gcgtcctgcc gatgctcgac gaggtcgaac gcgccggcga tgcccgcagc gtggtcaagg gaatcctgga ccgcggcgag aaactgatgg ggttcgggca ccgggtctac cgcgccgagg acccgcgggc gcgggtgctg cgggcggccg ccgagcggct gggcgccccc cgctacgagg tcgcggtcgc ggtggagcag gctgcgctgt cagaactgcg agagcgccgt ccggatcggg cgatcgagac caatgtcgaa ttctgggccg cagtggtcct ggactttgcc cgggtaccgg ccaacatgat gccagcaatg ttcacctgtg ggcgcactgc cggctggtgt gcccacattc tcgagcagaa gcgactcggc aagctggtcc gcccgtcggc catttacgtg ggaccgggcc cgcgcagccc ggaatcggtc gacggctggg agcgggtact caccaccgcc gctgggcacc accatcatca tcattaacgg a
Expected Protein Sequence
MTVVPENFVP GLDGVVAFTT EIAEPDKDGG ALRYRGVDIE DLVSQRVTFG DVWALLVDGN FGSGLPPAEP FPLPIHSGDV RVDVQAGLAM LAPIWGYAPL LDIDDATARQ QLARASVMAL SYVAQSARGI YQPAVPQRII DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MLDEVERAGD ARSVVKGILD RGEKLMGFGH RVYRAEDPRA RVLRAAAERL GAPRYEVAVA VEQAALSELR ERRPDRAIET NVEFWAAVVL DFARVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPGPRSP ESVDGWERVL TTAGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gacagtggtc ccggagaatt tcgtccccgg cctcgacggc gtggtggcct ttacgaccga gatcgccgag ccggacaaag acggcggggc cctgcgctac cgtggcgtcg acatcgaaga cctggtaagt cagcgggtca ccttcggcga tgtgtgggcg ctgctggtgg acggcaactt cggcagcggg ctgccgccgg ctgaaccgtt cccgctgccg attcactccg gcgatgtgcg cgtcgacgtc caggccggcc tggcgatgct ggcgcccatc tggggatatg cgccgctgct cgacatcgac gacgccaccg cccgccaaca gctggcccgg gcatcggtga tggcgctgtc ctatgtcgcg caatccgcac ggggcatcta ccagccggcg gtcccgcagc gaatcatcga tgaatgctcg acggtcacag cacgtttcat gacgcgctgg cagggcgagc cagaccccag acacatcgag gccattgacg cctactgggt gtcggccgcc gagcacggca tgaacgcttc gacattcacc gcgcgtgtga tcgcctcaac tggggcggat gtggcagcag cgctgtcggg cgcaatcggg gcgatgagcg gaccgctgca tggtggcgcg ccggcgcgcg tcctgccgat gctcgacgag gtcgaacgcg ccggcgatgc ccgcagcgtg gtcaagggaa tcctggaccg cggcgagaaa ctgatggggt tcgggcaccg ggtctaccgc gccgaggacc cgcgggcgcg ggtgctgcgg gcggccgccg agcggctggg cgccccccgc tacgaggtcg cggtcgcggt ggagcaggct gcgctgtcag aactgcgaga gcgccgtccg gatcgggcga tcgagaccaa tgtcgaattc tgggccgcag tggtcctgga ctttgcccgg gtaccggcca acatgatgcc agcaatgttc acctgtgggc gcactgccgg ctggtgtgcc cacattctcg agcagaagcg actcggcaag ctggtccgcc cgtcggccat ttacgtggga ccgggcccgc gcagcccgga atcggtcgac ggctgggagc gggtactcac caccgccggg caccaccatc atcatcatta acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat
Details for MyboA.00896.a.AE1.GE43403
| HARVESTED ON: | 9/18/2019 |
| SEQUENCED ON: | 10/1/2019 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MMTVVPENFV PGLDGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSQRVTF GDVWALLVDG NFGSGLPPAE PFPLPIHSGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR QQLARASVMA LSYVAQSARG IYQPAVPQRI IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMLDEVERAG DARSVVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRAAAER LGAPRYEVAV AVEQAALSEL RERRPDRAIE TNVEFWAAVV LDFARVPANM MPAMFTCGRT AGWCAHILEQ KRLGKLVRPS AIYVGPGPRS PESVDGWERV LTTAAGHHHH HH
Validated NT Sequence
tctctcccat gatgacagtg gtcccggaga atttcgtccc cggcctcgac ggcgtggtgg cctttacgac cgagatcgcc gagccggaca aagacggcgg ggccctgcgc taccgtggcg tcgacatcga agacctggta agtcagcggg tcaccttcgg cgatgtgtgg gcgctgctgg tggacggcaa cttcggcagc gggctgccgc cggctgaacc gttcccgctg ccgattcact ccggcgatgt gcgcgtcgac gtccaggccg gcctggcgat gctggcgccc atctggggat atgcgccgct gctcgacatc gacgacgcca ccgcccgcca acagctggcc cgggcatcgg tgatggcgct gtcctatgtc gcgcaatccg cacggggcat ctaccagccg gcggtcccgc agcgaatcat cgatgaatgc tcgacggtca cagcacgttt catgacgcgc tggcagggcg agccagaccc cagacacatc gaggccattg acgcctactg ggtgtcggcc gccgagcacg gcatgaacgc ttcgacattc accgcgcgtg tgatcgcctc aactggggcg gatgtggcag cagcgctgtc gggcgcaatc ggggcgatga gcggaccgct gcatggtggc gcgccggcgc gcgtcctgcc gatgctcgac gaggtcgaac gcgccggcga tgcccgcagc gtggtcaagg gaatcctgga ccgcggcgag aaactgatgg ggttcgggca ccgggtctac cgcgccgagg acccgcgggc gcgggtgctg cgggcggccg ccgagcggct gggcgccccc cgctacgagg tcgcggtcgc ggtggagcag gctgcgctgt cagaactgcg agagcgccgt ccggatcggg cgatcgagac caatgtcgaa ttctgggccg cagtggtcct ggactttgcc cgggtaccgg ccaacatgat gccagcaatg ttcacctgtg ggcgcactgc cggctggtgt gcccacattc tcgagcagaa gcgactcggc aagctggtcc gcccgtcggc catttacgtg ggaccgggcc cgcgcagccc ggaatcggtc gacggctggg agcgggtact caccaccgcc gctgggcacc accatcatca tcattaacgg a
Expected Protein Sequence
MTVVPENFVP GLDGVVAFTT EIAEPDKDGG ALRYRGVDIE DLVSQRVTFG DVWALLVDGN FGSGLPPAEP FPLPIHSGDV RVDVQAGLAM LAPIWGYAPL LDIDDATARQ QLARASVMAL SYVAQSARGI YQPAVPQRII DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MLDEVERAGD ARSVVKGILD RGEKLMGFGH RVYRAEDPRA RVLRAAAERL GAPRYEVAVA VEQAALSELR ERRPDRAIET NVEFWAAVVL DFARVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPGPRSP ESVDGWERVL TTAGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gacagtggtc ccggagaatt tcgtccccgg cctcgacggc gtggtggcct ttacgaccga gatcgccgag ccggacaaag acggcggggc cctgcgctac cgtggcgtcg acatcgaaga cctggtaagt cagcgggtca ccttcggcga tgtgtgggcg ctgctggtgg acggcaactt cggcagcggg ctgccgccgg ctgaaccgtt cccgctgccg attcactccg gcgatgtgcg cgtcgacgtc caggccggcc tggcgatgct ggcgcccatc tggggatatg cgccgctgct cgacatcgac gacgccaccg cccgccaaca gctggcccgg gcatcggtga tggcgctgtc ctatgtcgcg caatccgcac ggggcatcta ccagccggcg gtcccgcagc gaatcatcga tgaatgctcg acggtcacag cacgtttcat gacgcgctgg cagggcgagc cagaccccag acacatcgag gccattgacg cctactgggt gtcggccgcc gagcacggca tgaacgcttc gacattcacc gcgcgtgtga tcgcctcaac tggggcggat gtggcagcag cgctgtcggg cgcaatcggg gcgatgagcg gaccgctgca tggtggcgcg ccggcgcgcg tcctgccgat gctcgacgag gtcgaacgcg ccggcgatgc ccgcagcgtg gtcaagggaa tcctggaccg cggcgagaaa ctgatggggt tcgggcaccg ggtctaccgc gccgaggacc cgcgggcgcg ggtgctgcgg gcggccgccg agcggctggg cgccccccgc tacgaggtcg cggtcgcggt ggagcaggct gcgctgtcag aactgcgaga gcgccgtccg gatcgggcga tcgagaccaa tgtcgaattc tgggccgcag tggtcctgga ctttgcccgg gtaccggcca acatgatgcc agcaatgttc acctgtgggc gcactgccgg ctggtgtgcc cacattctcg agcagaagcg actcggcaag ctggtccgcc cgtcggccat ttacgtggga ccgggcccgc gcagcccgga atcggtcgac ggctgggagc gggtactcac caccgccggg caccaccatc atcatcatta acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat
Details for MyboA.00896.a.A1.PS00947
| PURIFICATION DATe: | 1/24/2011 |
| CONCENTRATION: | 21.96mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 80 |
Protocol Notes
notes unavailable
Validated AA Sequence
MCGRCWWTAT SAAGCRRLNR SRCRFTPAMC ASTSRPALAM LAPIWGYAPL LDIDDATARQ QLARASVMAL SYVAQSARGI YQPAVPQRII DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MLDEVERAGD ARSVVKGILD RGEKLMGFGH RVYRAEDPRA RVLRAAAERL GAPRYEVAVA VEQAALSELR ERRPDRAIET NVEFWAAVVL DFARVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPGPRSP ESVDGWERV
Validated NT Sequence
agtacccgct cccagccgtc gaccgattcc gggctgcgcg ggcccggtcc cacgtaaatg gccgacgggc ggaccagctt gccgagtcgc ttctgctcga gaatgtgggc acaccagccg gcagtgcgcc cacaggtgaa cattgctggc atcatgttgg ccggtacccg ggcaaagtcc aggaccactg cggcccagaa ttcgacattg gtctcgatcg cccgatccgg acggcgctct cgcagttctg acagcgcagc ctgctccacc gcgaccgcga cctcgtagcg gggggcgccc agccgctcgg cggccgcccg cagcacccgc gcccgcgggt cctcggcgcg gtagacccgg tgcccgaacc ccatcagttt ctcgccgcgg tccaggattc ccttgaccac gctgcgggca tcgccggcgc gttcgacctc gtcgagcatc ggcaggacgc gcgccggcgc gccaccatgc agcggtccgc tcatcgcccc gattgcgccc gacagcgctg ctgccacatc cgccccagtt gaggcgatca cacgcgcggt gaatgtcgaa gcgttcatgc cgtgctcggc ggccgacacc cagtaggcgt caatggcctc gatgtgtctg gggtctggct cgccctgcca gcgcgtcatg aaacgtgctg tgaccgtcga gcattcatcg atgattcgct gcgggaccgc cggctggtag atgccccgtg cggattgcgc gacataggac agcgccatca ccgatgcccg ggccagctgt tggcgggcgg tggcgtcgtc gatgtcgagc agcggcgcat atccccagat gggcgccagc atcgccaggg ccggcctgga cgtcgacgcg cacatcgccg gagtgaatcg gcagcgggaa cggttcagcc ggcggcagcc cgctgccgaa gttgccgtcc accagcagcg cccacacatc gccgaaggtg acccgctgac ttaccaggtc ttngangtcg acgccacggt agcgcagggc cccgccgtct ttgtccggct cggcgatctc ggtcgtaaag gccaccacgc cgtcgaggcc ggggacgaaa ttctccggga ccactgtcat cgaaccagga ccctgggtct gagctnccag ggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVPENFV PGLDGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSQRVTF GDVWALLVDG NFGSGLPPAE PFPLPIHSGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR QQLARASVMA LSYVAQSARG IYQPAVPQRI IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMLDEVERAG DARSVVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRAAAER LGAPRYEVAV AVEQAALSEL RERRPDRAIE TNVEFWAAVV LDFARVPANM MPAMFTCGRT AGWCAHILEQ KRLGKLVRPS AIYVGPGPRS PESVDGWERV LTTA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGTGGTC CCGGAGAATT TCGTCCCCGG CCTCGACGGC GTGGTGGCCT TTACGACCGA GATCGCCGAG CCGGACAAAG ACGGCGGGGC CCTGCGCTAC CGTGGCGTCG ACATCGAAGA CCTGGTAAGT CAGCGGGTCA CCTTCGGCGA TGTGTGGGCG CTGCTGGTGG ACGGCAACTT CGGCAGCGGG CTGCCGCCGG CTGAACCGTT CCCGCTGCCG ATTCACTCCG GCGATGTGCG CGTCGACGTC CAGGCCGGCC TGGCGATGCT GGCGCCCATC TGGGGATATG CGCCGCTGCT CGACATCGAC GACGCCACCG CCCGCCAACA GCTGGCCCGG GCATCGGTGA TGGCGCTGTC CTATGTCGCG CAATCCGCAC GGGGCATCTA CCAGCCGGCG GTCCCGCAGC GAATCATCGA TGAATGCTCG ACGGTCACAG CACGTTTCAT GACGCGCTGG CAGGGCGAGC CAGACCCCAG ACACATCGAG GCCATTGACG CCTACTGGGT GTCGGCCGCC GAGCACGGCA TGAACGCTTC GACATTCACC GCGCGTGTGA TCGCCTCAAC TGGGGCGGAT GTGGCAGCAG CGCTGTCGGG CGCAATCGGG GCGATGAGCG GACCGCTGCA TGGTGGCGCG CCGGCGCGCG TCCTGCCGAT GCTCGACGAG GTCGAACGCG CCGGCGATGC CCGCAGCGTG GTCAAGGGAA TCCTGGACCG CGGCGAGAAA CTGATGGGGT TCGGGCACCG GGTCTACCGC GCCGAGGACC CGCGGGCGCG GGTGCTGCGG GCGGCCGCCG AGCGGCTGGG CGCCCCCCGC TACGAGGTCG CGGTCGCGGT GGAGCAGGCT GCGCTGTCAG AACTGCGAGA GCGCCGTCCG GATCGGGCGA TCGAGACCAA TGTCGAATTC TGGGCCGCAG TGGTCCTGGA CTTTGCCCGG GTACCGGCCA ACATGATGCC AGCAATGTTC ACCTGTGGGC GCACTGCCGG CTGGTGTGCC CACATTCTCG AGCAGAAGCG ACTCGGCAAG CTGGTCCGCC CGTCGGCCAT TTACGTGGGA CCGGGCCCGC GCAGCCCGGA ATCGGTCGAC GGCTGGGAGC GGGTACTCAC CACCGCCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for MyboA.00896.a.AE1.PW38757
| PURIFICATION DATe: | 11/20/2019 |
| CONCENTRATION: | 23mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MMTVVPENFV PGLDGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSQRVTF GDVWALLVDG NFGSGLPPAE PFPLPIHSGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR QQLARASVMA LSYVAQSARG IYQPAVPQRI IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMLDEVERAG DARSVVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRAAAER LGAPRYEVAV AVEQAALSEL RERRPDRAIE TNVEFWAAVV LDFARVPANM MPAMFTCGRT AGWCAHILEQ KRLGKLVRPS AIYVGPGPRS PESVDGWERV LTTAAGHHHH HH
Validated NT Sequence
tctctcccat gatgacagtg gtcccggaga atttcgtccc cggcctcgac ggcgtggtgg cctttacgac cgagatcgcc gagccggaca aagacggcgg ggccctgcgc taccgtggcg tcgacatcga agacctggta agtcagcggg tcaccttcgg cgatgtgtgg gcgctgctgg tggacggcaa cttcggcagc gggctgccgc cggctgaacc gttcccgctg ccgattcact ccggcgatgt gcgcgtcgac gtccaggccg gcctggcgat gctggcgccc atctggggat atgcgccgct gctcgacatc gacgacgcca ccgcccgcca acagctggcc cgggcatcgg tgatggcgct gtcctatgtc gcgcaatccg cacggggcat ctaccagccg gcggtcccgc agcgaatcat cgatgaatgc tcgacggtca cagcacgttt catgacgcgc tggcagggcg agccagaccc cagacacatc gaggccattg acgcctactg ggtgtcggcc gccgagcacg gcatgaacgc ttcgacattc accgcgcgtg tgatcgcctc aactggggcg gatgtggcag cagcgctgtc gggcgcaatc ggggcgatga gcggaccgct gcatggtggc gcgccggcgc gcgtcctgcc gatgctcgac gaggtcgaac gcgccggcga tgcccgcagc gtggtcaagg gaatcctgga ccgcggcgag aaactgatgg ggttcgggca ccgggtctac cgcgccgagg acccgcgggc gcgggtgctg cgggcggccg ccgagcggct gggcgccccc cgctacgagg tcgcggtcgc ggtggagcag gctgcgctgt cagaactgcg agagcgccgt ccggatcggg cgatcgagac caatgtcgaa ttctgggccg cagtggtcct ggactttgcc cgggtaccgg ccaacatgat gccagcaatg ttcacctgtg ggcgcactgc cggctggtgt gcccacattc tcgagcagaa gcgactcggc aagctggtcc gcccgtcggc catttacgtg ggaccgggcc cgcgcagccc ggaatcggtc gacggctggg agcgggtact caccaccgcc gctgggcacc accatcatca tcattaacgg a
Expressed Protein Sequence
MTVVPENFVP GLDGVVAFTT EIAEPDKDGG ALRYRGVDIE DLVSQRVTFG DVWALLVDGN FGSGLPPAEP FPLPIHSGDV RVDVQAGLAM LAPIWGYAPL LDIDDATARQ QLARASVMAL SYVAQSARGI YQPAVPQRII DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MLDEVERAGD ARSVVKGILD RGEKLMGFGH RVYRAEDPRA RVLRAAAERL GAPRYEVAVA VEQAALSELR ERRPDRAIET NVEFWAAVVL DFARVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPGPRSP ESVDGWERVL TTAGHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GACAGTGGTC CCGGAGAATT TCGTCCCCGG CCTCGACGGC GTGGTGGCCT TTACGACCGA GATCGCCGAG CCGGACAAAG ACGGCGGGGC CCTGCGCTAC CGTGGCGTCG ACATCGAAGA CCTGGTAAGT CAGCGGGTCA CCTTCGGCGA TGTGTGGGCG CTGCTGGTGG ACGGCAACTT CGGCAGCGGG CTGCCGCCGG CTGAACCGTT CCCGCTGCCG ATTCACTCCG GCGATGTGCG CGTCGACGTC CAGGCCGGCC TGGCGATGCT GGCGCCCATC TGGGGATATG CGCCGCTGCT CGACATCGAC GACGCCACCG CCCGCCAACA GCTGGCCCGG GCATCGGTGA TGGCGCTGTC CTATGTCGCG CAATCCGCAC GGGGCATCTA CCAGCCGGCG GTCCCGCAGC GAATCATCGA TGAATGCTCG ACGGTCACAG CACGTTTCAT GACGCGCTGG CAGGGCGAGC CAGACCCCAG ACACATCGAG GCCATTGACG CCTACTGGGT GTCGGCCGCC GAGCACGGCA TGAACGCTTC GACATTCACC GCGCGTGTGA TCGCCTCAAC TGGGGCGGAT GTGGCAGCAG CGCTGTCGGG CGCAATCGGG GCGATGAGCG GACCGCTGCA TGGTGGCGCG CCGGCGCGCG TCCTGCCGAT GCTCGACGAG GTCGAACGCG CCGGCGATGC CCGCAGCGTG GTCAAGGGAA TCCTGGACCG CGGCGAGAAA CTGATGGGGT TCGGGCACCG GGTCTACCGC GCCGAGGACC CGCGGGCGCG GGTGCTGCGG GCGGCCGCCG AGCGGCTGGG CGCCCCCCGC TACGAGGTCG CGGTCGCGGT GGAGCAGGCT GCGCTGTCAG AACTGCGAGA GCGCCGTCCG GATCGGGCGA TCGAGACCAA TGTCGAATTC TGGGCCGCAG TGGTCCTGGA CTTTGCCCGG GTACCGGCCA ACATGATGCC AGCAATGTTC ACCTGTGGGC GCACTGCCGG CTGGTGTGCC CACATTCTCG AGCAGAAGCG ACTCGGCAAG CTGGTCCGCC CGTCGGCCAT TTACGTGGGA CCGGGCCCGC GCAGCCCGGA ATCGGTCGAC GGCTGGGAGC GGGTACTCAC CACCGCCGGG CACCACCATC ATCATCATTA ACGGATCCGA ATTCGAGCTC CGTCGACAAG CTTGCGGCCG CACTCGAGCA CCACCACCAC CACCACTGAG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGAT