MyboA.00609.a

PROBABLE GLUTAMINE-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER GLNQ

CENTER ID: MyboA.00609.a
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.00609.a.A1.GE30562 Full length( MyboA.00609.a ) 1 280
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.85
RefSeq: NP_853743.1
UniProt: A0A0H3P200

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGDLSIQNLV VEYYSGGYAL RPINGLNLDV AAGSLVMLLG PSGCGKTTLL SCLGGILRPK SGAIKFDEVD ITTLQGAELA NYRRNKVGIV FQAFNLVPSL TAVENVMVPL RSAGMSRRAS RRRAEELLAR VNLAERMNHR PGDLSGGQQQ RVAVARAIAL DPPLILADEP TAHLDFIQVE EVLRLIRELA DGERVVVVAT HDSRMLPMAD RVVELTPDFA ETNRPPETVH LQAGEVLFEQ STMGDLIYVV SEGEFEIVHD WPTAVRNWSR LPGRGITSAR
NT Sequence
GTGGGCGATC TCAGCATTCA GAACCTCGTC GTTGAGTACT ACAGCGGTGG ATACGCGCTT AGGCCGATCA ACGGTTTGAA CCTCGACGTG GCAGCCGGGT CGTTGGTGAT GCTGCTCGGA CCCAGCGGCT GCGGCAAGAC GACACTGCTT TCCTGTCTGG GCGGCATTCT GCGCCCGAAG TCTGGGGCGA TCAAGTTCGA CGAAGTCGAC ATCACGACGC TACAAGGCGC CGAGCTGGCG AACTACCGGC GTAACAAGGT CGGCATCGTG TTCCAGGCGT TCAATCTGGT GCCCAGCCTG ACCGCTGTCG AGAACGTGAT GGTGCCGTTA CGCTCGGCCG GGATGTCACG CAGGGCGTCG CGTAGGCGTG CCGAAGAACT GCTGGCGCGC GTCAATCTCG CGGAACGAAT GAATCATCGA CCCGGTGATC TGAGCGGAGG TCAGCAGCAA CGAGTCGCGG TGGCACGCGC GATTGCGCTG GATCCGCCAC TGATCCTCGC TGACGAACCG ACCGCACACC TGGATTTCAT CCAGGTGGAG GAGGTGCTGC GGTTGATCCG CGAACTGGCC GATGGCGAGC GTGTGGTCGT GGTCGCAACC CACGACAGCA GGATGTTGCC GATGGCCGAT CGCGTCGTTG AGCTGACACC CGATTTCGCG GAGACAAATC GGCCACCTGA AACCGTACAT CTTCAGGCCG GCGAGGTGCT GTTCGAGCAG AGCACGATGG GCGACCTGAT CTACGTGGTG TCGGAGGGCG AGTTTGAGAT TGTGCACGAT TGGCCGACGG CGGTGAGGAA TTGGTCAAGG TTGCCGGGCC GGGGGATTAC TTCGGCGAGA
Details for MyboA.00609.a.A1.GE30562
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 109
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGDLSIQN LVVEYYSGGY ALRPINGLNL DVAAGSLVML LGPSGCGKTT LLSCLGGILR PKSGAIKFDE VDITTLQGAE LANYRRNKVG IVFQAFNLVP SLTAVENVMV PLRSAGMSRR ASRRRAEELL ARVNLAERMN HRPGDLSGGQ QQRVAVARAI ALDPPLILAD EPTAHLDFIQ VEEVLRLIRE LADGERVVVV ATHDSRMLPM ADRVVELTPD FAETNRPPET VHLXAGEVLF EQXTMGDLIY VVSXGEFEIV HXWPXAVRNW SXLPGRGITS ARXKQHXQXL QPXFSRXXAX KXXXEAXLAA X
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gcgatctcag cattcagaac ctcgtcgttg agtactacag cggtggatac gcgcttaggc cgatcaacgg tttgaacctc gacgtggcag ccgggtcgtt ggtgatgctg ctcggaccca gcggctgcgg caagacgaca ctgctttcct gtctgggcgg cattctgcgc ccgaagtctg gggcgatcaa gttcgacgaa gtcgacatca cgacgctaca aggcgccgag ctggcgaact accggcgtaa caaggtcggc atcgtgttcc aggcgttcaa tctggtgccc agcctgaccg ctgtcgagaa cgtgatggtg ccgttacgct cggccgggat gtcacgcagg gcgtcgcgta ggcgtgccga agaactgctg gcgcgcgtca atctcgcgga acgaatgaat catcgacccg gtgatctgag cggaggtcag cagcaacgag tcgcggtggc acgcgcgatt gcgctggatc cgccactgat cctcgctgac gaaccgaccg cacacctgga tttcatccag gtggaggagg tgctgcggtt gatccgcgaa ctggccgatg gcgagcgtgt ggtcgtggtc gcaacccacg acagcaggat gttgccgatg gccgatcgcg tcgttgagct gacacccgat ttcgcggaga caaatcggcc acctgaaacc gtacatcttc angccggcga ggtgctgttc gagcaganca cgatgggcga cctgatctac gtggtgtcgg anggcgagtt tgagattgtg cacgantggc cganggcggt gaggaattgg tcanngttgc cgggccgggg gattacttcn gcgaganata aacagcacgn acaagntctg cagccnngct tctcgaggan nnncgctanc aaagncngan nngaagctga nttggctgct gnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGDLSIQN LVVEYYSGGY ALRPINGLNL DVAAGSLVML LGPSGCGKTT LLSCLGGILR PKSGAIKFDE VDITTLQGAE LANYRRNKVG IVFQAFNLVP SLTAVENVMV PLRSAGMSRR ASRRRAEELL ARVNLAERMN HRPGDLSGGQ QQRVAVARAI ALDPPLILAD EPTAHLDFIQ VEEVLRLIRE LADGERVVVV ATHDSRMLPM ADRVVELTPD FAETNRPPET VHLQAGEVLF EQSTMGDLIY VVSEGEFEIV HDWPTAVRNW SRLPGRGITS AR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggcgat ctcagcattc agaacctcgt cgttgagtac tacagcggtg gatacgcgct taggccgatc aacggtttga acctcgacgt ggcagccggg tcgttggtga tgctgctcgg acccagcggc tgcggcaaga cgacactgct ttcctgtctg ggcggcattc tgcgcccgaa gtctggggcg atcaagttcg acgaagtcga catcacgacg ctacaaggcg ccgagctggc gaactaccgg cgtaacaagg tcggcatcgt gttccaggcg ttcaatctgg tgcccagcct gaccgctgtc gagaacgtga tggtgccgtt acgctcggcc gggatgtcac gcagggcgtc gcgtaggcgt gccgaagaac tgctggcgcg cgtcaatctc gcggaacgaa tgaatcatcg acccggtgat ctgagcggag gtcagcagca acgagtcgcg gtggcacgcg cgattgcgct ggatccgcca ctgatcctcg ctgacgaacc gaccgcacac ctggatttca tccaggtgga ggaggtgctg cggttgatcc gcgaactggc cgatggcgag cgtgtggtcg tggtcgcaac ccacgacagc aggatgttgc cgatggccga tcgcgtcgtt gagctgacac ccgatttcgc ggagacaaat cggccacctg aaaccgtaca tcttcaggcc ggcgaggtgc tgttcgagca gagcacgatg ggcgacctga tctacgtggt gtcggagggc gagtttgaga ttgtgcacga ttggccgacg gcggtgagga attggtcaag gttgccgggc cgggggatta cttcggcgag ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa