MyboA.00458.a

Probable acyl-CoA thioesterase II tesB1

CENTER ID: MyboA.00458.a
ORGANISM: Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyboA.00458.a.A1.GE30561 Full length( MyboA.00458.a ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|233413.5.peg.1795
RefSeq: NP_855297.1
UniProt: A0A0H3P3B0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDGKPMSDF DELLAVLDLN AVASDLFTGS HPSKNPLRTF GGQLMAQSFV ASSRTLTRHH LPPSAFSVHF INGGDTAKDI EFQVIRLRDE RRFANRRVDA VQDGTLLSSA MVSYMAGGRG LEHALDPPQV AEPHTRPPIG ELLRGYEETV PHFVNALQPI EWRYANDPAW IMRDKGDRLA YNRVWVKALG EMPDDPVLHT ATLLYSSDTT VLDSVITTHG LSWGFDRIFA ASANHSVWFH RQVNFDDWVL YSTSSPVAAD SRGLGSGHFF DRSGKLIATV VQEGVLKYFP ATPDSAAGRS
NT Sequence
GTGCCGGACG GTAAACCCAT GTCCGACTTC GATGAACTAC TGGCGGTATT GGACCTCAAC GCCGTCGCAA GCGACCTGTT CACCGGATCC CACCCCAGCA AAAACCCGCT CCGGACATTT GGTGGCCAGC TCATGGCGCA GTCATTCGTC GCGAGCAGCC GAACGCTAAC CCGCCACCAC CTACCGCCCA GCGCATTCTC GGTGCACTTC ATCAACGGCG GTGACACGGC CAAGGACATC GAGTTCCAGG TGATACGACT GCGCGATGAG CGGCGCTTCG CCAACCGGCG CGTCGATGCG GTACAGGACG GCACGTTGCT GTCCTCGGCG ATGGTGTCTT ACATGGCCGG TGGTCGCGGG CTCGAGCATG CGCTGGATCC GCCGCAGGTG GCCGAGCCTC ATACCCGGCC GCCGATCGGT GAGCTGTTGC GCGGTTACGA GGAGACCGTC CCGCATTTTG TCAACGCGCT GCAACCGATC GAATGGCGCT ACGCCAACGA CCCGGCCTGG ATAATGCGGG ACAAGGGCGA TCGGCTTGCC TACAACCGGG TCTGGGTCAA GGCACTAGGG GAGATGCCCG ACGACCCGGT GCTGCACACG GCGACACTGT TGTACTCCTC GGACACCACC GTGCTGGACT CGGTCATTAC CACCCATGGT CTGTCCTGGG GCTTCGATCG CATCTTTGCG GCCTCTGCCA ACCACTCGGT GTGGTTTCAC CGGCAGGTCA ACTTCGATGA TTGGGTGCTC TACTCGACGT CGTCACCGGT GGCCGCCGAT TCACGTGGGT TGGGTTCGGG GCACTTTTTT GATCGCTCGG GGAAGCTCAT CGCAACTGTG GTGCAGGAAG GTGTGTTGAA GTATTTTCCC GCCACCCCTG ACAGTGCGGC AGGACGCTCG
Details for MyboA.00458.a.A1.GE30561
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPDGKPMS DFDELLAVLD LNAVASDLFT GSHPSKNPLR TFGGQLMAQS FVASSRTLTR HHLPPSAFSV HFINGGDTAK DIEFQVIRLR DERRFANRRV DAVQDGTLLS SAMVSYMAGG RGLEHALDPP QVAEPHTRPP IGELLRGYEE TVPHFVNALQ PIEWRYANDP AWIMRDKGDR LAYNRVWVKA LGEMPDDPVL HTATLLYSSD TTVLDSVITT HGLSWGFDRI FAASANHSVW FHRQVNFDDW VLYSTSSPVA ADSRGLGSGH FFDRSGKLIA TVVQEGVLKY FPATPDSAAG RS
Validated NT Sequence
ttattacgag cgtcctgccg cactgtcagg ggtggcggga aaatacttca acacaccttc ctgcaccaca gttgcgatga gcttccccga gcgatcaaaa aagtgccccg aacccaaccc acgtgaatcg gcggccaccg gtgacgacgt cgagtagagc acccaatcat cgaagttgac ctgccggtga aaccacaccg agtggttggc agaggccgca aagatgcgat cgaagcccca ggacagacca tgggtggtaa tgaccgagtc cagcacggtg gtgtccgagg agtacaacag tgtcgccgtg tgcagcaccg ggtcgtcggg catctcccct agtgccttga cccagacccg gttgtaggca agccgatcgc ccttgtcccg cattatccag gccgggtcgt tggcgtagcg ccattcgatc ggttgcagcg cgttgacaaa atgcgggacg gtctcctcgt aaccgcgcaa cagctcaccg atcggcggcc gggtatgagg ctcggccacc tgcggcggat ccagcgcatg ctcgagcccg cgaccaccgg ccatgtaaga caccatcgcc gaggacagca acgtgccgtc ctgtaccgca tcgacgcgcc ggttggcgaa gcgccgctca tcgcgcagtc gtatcacctg gaactcgatg tccttggccg tgtcaccgcc gttgatgaag tgcaccgaga atgcgctggg cggtaggtgg tggcgggtta gcgttcggct gctcgcgacg aatgactgcg ccatgagctg gccaccaaat gtccggagcg ggtttttgct ggggtgggat ccggtgaaca ggtcgcttgc gacggcgttg aggtccaata ccgccagtag ttcatcgaag tcggacatgg gtttaccgtc cggcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPDGKPMS DFDELLAVLD LNAVASDLFT GSHPSKNPLR TFGGQLMAQS FVASSRTLTR HHLPPSAFSV HFINGGDTAK DIEFQVIRLR DERRFANRRV DAVQDGTLLS SAMVSYMAGG RGLEHALDPP QVAEPHTRPP IGELLRGYEE TVPHFVNALQ PIEWRYANDP AWIMRDKGDR LAYNRVWVKA LGEMPDDPVL HTATLLYSSD TTVLDSVITT HGLSWGFDRI FAASANHSVW FHRQVNFDDW VLYSTSSPVA ADSRGLGSGH FFDRSGKLIA TVVQEGVLKY FPATPDSAAG RS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccggac ggtaaaccca tgtccgactt cgatgaacta ctggcggtat tggacctcaa cgccgtcgca agcgacctgt tcaccggatc ccaccccagc aaaaacccgc tccggacatt tggtggccag ctcatggcgc agtcattcgt cgcgagcagc cgaacgctaa cccgccacca cctaccgccc agcgcattct cggtgcactt catcaacggc ggtgacacgg ccaaggacat cgagttccag gtgatacgac tgcgcgatga gcggcgcttc gccaaccggc gcgtcgatgc ggtacaggac ggcacgttgc tgtcctcggc gatggtgtct tacatggccg gtggtcgcgg gctcgagcat gcgctggatc cgccgcaggt ggccgagcct catacccggc cgccgatcgg tgagctgttg cgcggttacg aggagaccgt cccgcatttt gtcaacgcgc tgcaaccgat cgaatggcgc tacgccaacg acccggcctg gataatgcgg gacaagggcg atcggcttgc ctacaaccgg gtctgggtca aggcactagg ggagatgccc gacgacccgg tgctgcacac ggcgacactg ttgtactcct cggacaccac cgtgctggac tcggtcatta ccacccatgg tctgtcctgg ggcttcgatc gcatctttgc ggcctctgcc aaccactcgg tgtggtttca ccggcaggtc aacttcgatg attgggtgct ctactcgacg tcgtcaccgg tggccgccga ttcacgtggg ttgggttcgg ggcacttttt tgatcgctcg gggaagctca tcgcaactgt ggtgcaggaa ggtgtgttga agtattttcc cgccacccct gacagtgcgg caggacgctc gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa