SSGCID: Target Page

MyboA.00358.e

POSSIBLE ENOYL-COA HYDRATASE ECHA13 (ENOYL HYDRASE) (UNSATURATED ACYL-COA HYDRATASE) (CROTONASE)

CENTER ID:	MyboA.00358.e
ORGANISM:	Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyboA.00358.e.A1.GE30559	Full length( MyboA.00358.e )		1	318

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyboA.00358.e.A1.PS00888	Full length( MyboA.00358.e )		1	318

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|233413.5.peg.2163
RefSeq:	NP_855620.1
UniProt:	Q83XB5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFVGRVGPVD RRSDGERSRR PREFEYIRYE TIDDGRIAAI TLDRPKQRNA QTRGMLVELG AAFELAEADD TVRVVILRAA GPAFSAGHDL GSADDIRERS PGPDQHPSYR CNGATFGGVE SRNRQEWHYY FENTKRWRNL RKITIAQVHG AVLSAGLMLA WCCDLIVASE DTVFADVVGT RLGMCGVEYF GHPWEFGPRK TKELLLTGDC IGADEAHALG MVSKVFPADE LATSTIEFAR RIAKVPTMAA LLIKESVNQT VDAMGFSAAL DGCFKIHQLN HAHWGEVTGG KLSYGTVEYG LEDWRAAPQI RPAIKQRP

NT Sequence

ATGTTCGTCG GCAGGGTCGG TCCGGTCGAT CGCCGGTCGG ACGGGGAACG CTCCCGACGG CCCCGGGAGT TCGAATACAT CCGCTACGAA ACCATCGACG ATGGGCGCAT CGCCGCGATC ACCCTGGACC GCCCGAAACA ACGCAACGCC CAGACCCGCG GCATGCTGGT CGAGCTGGGC GCCGCCTTCG AACTTGCCGA GGCGGACGAC ACCGTCCGGG TGGTGATCCT GCGGGCCGCC GGCCCCGCCT TCTCCGCCGG TCACGACCTC GGATCCGCTG ACGATATCCG GGAACGCTCG CCCGGGCCGG ACCAGCACCC CAGCTACCGG TGCAACGGGG CGACCTTCGG CGGGGTCGAG TCACGCAACC GCCAGGAGTG GCACTACTAC TTCGAAAACA CCAAGCGGTG GCGCAACCTG CGCAAGATCA CCATCGCCCA GGTGCACGGG GCCGTGCTGT CGGCGGGGTT GATGCTGGCC TGGTGCTGCG ATCTGATCGT CGCCAGCGAG GACACCGTGT TCGCCGATGT GGTCGGCACC CGGCTGGGCA TGTGCGGGGT CGAGTACTTC GGACATCCGT GGGAGTTCGG GCCGCGCAAG ACCAAGGAAC TGCTGCTCAC CGGCGACTGC ATAGGCGCCG ACGAGGCCCA CGCGCTGGGG ATGGTCAGCA AGGTGTTTCC CGCTGACGAA CTCGCGACCA GCACAATCGA ATTCGCGCGT CGGATCGCGA AGGTGCCGAC GATGGCGGCA CTGCTGATCA AGGAATCGGT GAACCAAACC GTCGATGCCA TGGGGTTTTC CGCCGCGCTG GACGGTTGCT TCAAGATCCA CCAGCTCAAT CACGCGCACT GGGGCGAAGT CACCGGCGGC AAGCTGTCCT ACGGAACGGT CGAGTACGGC CTGGAGGACT GGCGCGCCGC ACCGCAGATC CGGCCCGCGA TCAAGCAGCG GCCC

Details for MyboA.00358.e.A1.GE30559

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattagggc cgctgcttga tcgcgggccg gatctgcggt gcggcgcgcc agtcctccag gccgtactcg accgttccgt aggacagctt gccgccggtg acttcgcccc agtgcgcgtg attgagctgg tggatcttga agcaaccgtc cagcgcggcg gaaaacccca tggcatcgac ggtttggttc accgattcct tgatcagcag tgccgccatc gtcggcacct tcgcgatccg acgcgcgaat tcgattgtgc tggtcgcgag ttcgtcagcg ggaaacacct tgctgaccat ccccagcgcg tgggcctcgt cggcgcctat gcagtcgccg gtgagcagca gttccttggt cttgcgcggc ccgaactccc acggatgtcc gaagtactcg accccgcaca tgcccagccg ggtgccgacc acatcggcga acacggtgtc ctcgctggcg acgatcagat cgcagcacca ggccagcatc aaccccgccg acagcacggc cccgtgcacc tgggcgatgg tgatcttgcg caggttgcgc caccgcttgg tgttttcgaa gtagtagtgc cactcctggc ggttgcgtga ctcgaccccg ccgaaggtcg ccccgttgca ccggtagctg gggtgctggt ccggcccggg cgagcgttcc cggatatcgt cagcggatcc gaggtcgtga ccggcggaga aggcggggcc ggcggcccgc aggatcacca cccggacggt gtcgtccgcc tcggcaagtt cgaaggcggc gcccagctcg accagcatgc cgcgggtctg ggcgttgcgt tgtttcgggc ggtccagggt gatcgcggcg atgcgcccat cgtcgatggt ttcgtagcgg atgtattcga actcccgggg ccgtcgggag cgttccccgt ccgaccggcg atcgaccgga ccgaccctgc cgacgaacat cgaaccagga ccctgggtct gagcttcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMFVGRVGPV DRRSDGERSR RPREFEYIRY ETIDDGRIAA ITLDRPKQRN AQTRGMLVEL GAAFELAEAD DTVRVVILRA AGPAFSAGHD LGSADDIRER SPGPDQHPSY RCNGATFGGV ESRNRQEWHY YFENTKRWRN LRKITIAQVH GAVLSAGLML AWCCDLIVAS EDTVFADVVG TRLGMCGVEY FGHPWEFGPR KTKELLLTGD CIGADEAHAL GMVSKVFPAD ELATSTIEFA RRIAKVPTMA ALLIKESVNQ TVDAMGFSAA LDGCFKIHQL NHAHWGEVTG GKLSYGTVEY GLEDWRAAPQ IRPAIKQRP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcgtcggc agggtcggtc cggtcgatcg ccggtcggac ggggaacgct cccgacggcc ccgggagttc gaatacatcc gctacgaaac catcgacgat gggcgcatcg ccgcgatcac cctggaccgc ccgaaacaac gcaacgccca gacccgcggc atgctggtcg agctgggcgc cgccttcgaa cttgccgagg cggacgacac cgtccgggtg gtgatcctgc gggccgccgg ccccgccttc tccgccggtc acgacctcgg atccgctgac gatatccggg aacgctcgcc cgggccggac cagcacccca gctaccggtg caacggggcg accttcggcg gggtcgagtc acgcaaccgc caggagtggc actactactt cgaaaacacc aagcggtggc gcaacctgcg caagatcacc atcgcccagg tgcacggggc cgtgctgtcg gcggggttga tgctggcctg gtgctgcgat ctgatcgtcg ccagcgagga caccgtgttc gccgatgtgg tcggcacccg gctgggcatg tgcggggtcg agtacttcgg acatccgtgg gagttcgggc cgcgcaagac caaggaactg ctgctcaccg gcgactgcat aggcgccgac gaggcccacg cgctggggat ggtcagcaag gtgtttcccg ctgacgaact cgcgaccagc acaatcgaat tcgcgcgtcg gatcgcgaag gtgccgacga tggcggcact gctgatcaag gaatcggtga accaaaccgt cgatgccatg gggttttccg ccgcgctgga cggttgcttc aagatccacc agctcaatca cgcgcactgg ggcgaagtca ccggcggcaa gctgtcctac ggaacggtcg agtacggcct ggaggactgg cgcgccgcac cgcagatccg gcccgcgatc aagcagcggc cctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MyboA.00358.e.A1.PS00888

PURIFICATION DATe:	12/23/2010
CONCENTRATION:	28.22mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCGTCGGC AGGGTCGGTC CGGTCGATCG CCGGTCGGAC GGGGAACGCT CCCGACGGCC CCGGGAGTTC GAATACATCC GCTACGAAAC CATCGACGAT GGGCGCATCG CCGCGATCAC CCTGGACCGC CCGAAACAAC GCAACGCCCA GACCCGCGGC ATGCTGGTCG AGCTGGGCGC CGCCTTCGAA CTTGCCGAGG CGGACGACAC CGTCCGGGTG GTGATCCTGC GGGCCGCCGG CCCCGCCTTC TCCGCCGGTC ACGACCTCGG ATCCGCTGAC GATATCCGGG AACGCTCGCC CGGGCCGGAC CAGCACCCCA GCTACCGGTG CAACGGGGCG ACCTTCGGCG GGGTCGAGTC ACGCAACCGC CAGGAGTGGC ACTACTACTT CGAAAACACC AAGCGGTGGC GCAACCTGCG CAAGATCACC ATCGCCCAGG TGCACGGGGC CGTGCTGTCG GCGGGGTTGA TGCTGGCCTG GTGCTGCGAT CTGATCGTCG CCAGCGAGGA CACCGTGTTC GCCGATGTGG TCGGCACCCG GCTGGGCATG TGCGGGGTCG AGTACTTCGG ACATCCGTGG GAGTTCGGGC CGCGCAAGAC CAAGGAACTG CTGCTCACCG GCGACTGCAT AGGCGCCGAC GAGGCCCACG CGCTGGGGAT GGTCAGCAAG GTGTTTCCCG CTGACGAACT CGCGACCAGC ACAATCGAAT TCGCGCGTCG GATCGCGAAG GTGCCGACGA TGGCGGCACT GCTGATCAAG GAATCGGTGA ACCAAACCGT CGATGCCATG GGGTTTTCCG CCGCGCTGGA CGGTTGCTTC AAGATCCACC AGCTCAATCA CGCGCACTGG GGCGAAGTCA CCGGCGGCAA GCTGTCCTAC GGAACGGTCG AGTACGGCCT GGAGGACTGG CGCGCCGCAC CGCAGATCCG GCCCGCGATC AAGCAGCGGC CCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA