SSGCID: Target Page

MyboA.00358.d

ENOYL-COA HYDRATASE ECHA2 (ENOYL HYDRASE) (UNSATURATED ACYL-COA HYDRATASE) (CROTONASE)

CENTER ID:	MyboA.00358.d
ORGANISM:	Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyboA.00358.d.A1.GE30558	Full length( MyboA.00358.d )		1	304

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyboA.00358.d.A1.PS00939	Full length( MyboA.00358.d )		1	304

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|233413.5.peg.505
RefSeq:	NP_854127.1
UniProt:	A0A0H3P1U4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPTPDFQTLL YTTAGPVATI TLNRPEQLNT IVPPMPDEIE AAIGLAERDQ DIKVIVLRGA GRAFSGGYDF GGGFQHWGDA MMTDGRWDPG KDFAMVTARE TGPTQKFMAI WRASKPVIAQ VHGWCVGGAS DYALCADIVI ASEDAVIGTP YSRMWGAYLT GMWLYRLSLA KVKWHSLTGR PLTGVQAAEA ELINEAVPFE RLEARVAEIA TELARIPLSQ LQAQKLIVNQ AYENMGLAST QLLGGILDGL MRNTPDALEF IRTAQTQGVR AAVERRDGPF GDYSQAPPEL RPDPTHVITP DGSM

NT Sequence

ATGCCGACAC CCGATTTCCA GACGCTGCTG TACACGACGG CCGGGCCGGT GGCCACCATC ACGCTCAACC GCCCGGAACA GCTCAACACC ATCGTCCCGC CCATGCCCGA CGAGATCGAG GCCGCTATCG GGTTGGCCGA GCGCGACCAG GACATCAAGG TCATCGTGCT GCGCGGTGCC GGCCGCGCCT TCTCCGGCGG TTACGACTTC GGCGGCGGCT TCCAGCATTG GGGCGATGCC ATGATGACCG ACGGCCGATG GGATCCGGGC AAGGATTTCG CCATGGTCAC CGCGCGGGAG ACCGGACCGA CGCAGAAATT CATGGCCATC TGGCGGGCGT CCAAACCGGT GATCGCGCAA GTGCATGGTT GGTGCGTCGG CGGGGCCAGC GACTACGCGC TGTGTGCCGA CATTGTGATC GCCAGCGAGG ACGCCGTGAT CGGGACCCCG TATAGCCGCA TGTGGGGAGC CTATTTGACC GGGATGTGGC TGTATCGACT CAGCCTTGCC AAGGTCAAAT GGCACTCGCT GACGGGCCGG CCGCTGACCG GTGTGCAGGC CGCCGAAGCC GAGCTGATCA ACGAGGCGGT GCCGTTCGAG CGGCTCGAGG CTCGCGTCGC CGAGATCGCC ACCGAGCTGG CACGAATCCC GTTGTCACAG TTGCAAGCCC AGAAACTGAT CGTCAACCAG GCCTACGAGA ACATGGGCCT GGCCTCCACC CAGCTGCTGG GCGGCATTCT CGACGGGCTG ATGCGCAACA CCCCCGACGC GCTCGAGTTC ATCCGGACCG CCCAAACCCA GGGTGTGCGA GCCGCGGTCG AGCGCCGCGA CGGCCCGTTC GGCGACTACA GCCAAGCCCC ACCGGAACTG CGACCCGACC CCACGCACGT CATCACTCCT GATGGGAGCA TG

Details for MyboA.00358.d.A1.GE30558

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattacatg ctcccatcag gagtgatgac gtgcgtgggg tcgggtcgca gttccggtgg ggcttggctg tagtcgccga acgggccgtc gcggcgctcg accgcggctc gcacaccctg ggtttgggcg gtccggatga actcgagcgc gtcgggggtg ttgcgcatca gcccgtcgag aatgccgccc agcagctggg tggaggccag gcccatgttc tcgtaggcct ggttgacgat cagtttctgg gcttgcaact gtgacaacgg gattcgtgcc agctcggtgg cgatctcggc gacgcgagcc tcgagccgct cgaacggcac cgcctcgttg atcagctcgg cttcggcggc ctgcacaccg gtcagcggcc ggcccgtcag cgagtgccat ttgaccttgg caaggctgag tcgatacagc cacatcccgg tcaaataggc tccccacatg cggctatacg gggtcccgat cacggcgtcc tcgctggcga tcacaatgtc ggcacacagc gcgtagtcgc tggccccgcc gacgcaccaa ccatgcactt gcgcgatcac cggtttggac gcccgccaga tggccatgaa tttctgcgtc ggtccggtct cccgcgcggt gaccatggcg aaatccttgc ccggatccca tcggccgtcg gtcatcatgg catcgcccca atgctggaag ccgccgccga agtcgtaacc gccggagaag gcgcggccgg caccgcgcag cacgatgacc ttgatgtcct ggtcgcgctc ggccaacccg atagcggcct cgatctcgtc gggcatgggc gggacgatgg tgttgagctg ttccgggcgg ttgagcgtga tggtggccac cggcccggcc gtcgtgtaca gcagcgtctg gaaatcgggt gtcggcatcg aaccaggacc ctgggtctga gcttcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPTPDFQTL LYTTAGPVAT ITLNRPEQLN TIVPPMPDEI EAAIGLAERD QDIKVIVLRG AGRAFSGGYD FGGGFQHWGD AMMTDGRWDP GKDFAMVTAR ETGPTQKFMA IWRASKPVIA QVHGWCVGGA SDYALCADIV IASEDAVIGT PYSRMWGAYL TGMWLYRLSL AKVKWHSLTG RPLTGVQAAE AELINEAVPF ERLEARVAEI ATELARIPLS QLQAQKLIVN QAYENMGLAS TQLLGGILDG LMRNTPDALE FIRTAQTQGV RAAVERRDGP FGDYSQAPPE LRPDPTHVIT PDGSM

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgacaccc gatttccaga cgctgctgta cacgacggcc gggccggtgg ccaccatcac gctcaaccgc ccggaacagc tcaacaccat cgtcccgccc atgcccgacg agatcgaggc cgctatcggg ttggccgagc gcgaccagga catcaaggtc atcgtgctgc gcggtgccgg ccgcgccttc tccggcggtt acgacttcgg cggcggcttc cagcattggg gcgatgccat gatgaccgac ggccgatggg atccgggcaa ggatttcgcc atggtcaccg cgcgggagac cggaccgacg cagaaattca tggccatctg gcgggcgtcc aaaccggtga tcgcgcaagt gcatggttgg tgcgtcggcg gggccagcga ctacgcgctg tgtgccgaca ttgtgatcgc cagcgaggac gccgtgatcg ggaccccgta tagccgcatg tggggagcct atttgaccgg gatgtggctg tatcgactca gccttgccaa ggtcaaatgg cactcgctga cgggccggcc gctgaccggt gtgcaggccg ccgaagccga gctgatcaac gaggcggtgc cgttcgagcg gctcgaggct cgcgtcgccg agatcgccac cgagctggca cgaatcccgt tgtcacagtt gcaagcccag aaactgatcg tcaaccaggc ctacgagaac atgggcctgg cctccaccca gctgctgggc ggcattctcg acgggctgat gcgcaacacc cccgacgcgc tcgagttcat ccggaccgcc caaacccagg gtgtgcgagc cgcggtcgag cgccgcgacg gcccgttcgg cgactacagc caagccccac cggaactgcg acccgacccc acgcacgtca tcactcctga tgggagcatg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for MyboA.00358.d.A1.PS00939

PURIFICATION DATe:	1/18/2011
CONCENTRATION:	46.62mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGACACCC GATTTCCAGA CGCTGCTGTA CACGACGGCC GGGCCGGTGG CCACCATCAC GCTCAACCGC CCGGAACAGC TCAACACCAT CGTCCCGCCC ATGCCCGACG AGATCGAGGC CGCTATCGGG TTGGCCGAGC GCGACCAGGA CATCAAGGTC ATCGTGCTGC GCGGTGCCGG CCGCGCCTTC TCCGGCGGTT ACGACTTCGG CGGCGGCTTC CAGCATTGGG GCGATGCCAT GATGACCGAC GGCCGATGGG ATCCGGGCAA GGATTTCGCC ATGGTCACCG CGCGGGAGAC CGGACCGACG CAGAAATTCA TGGCCATCTG GCGGGCGTCC AAACCGGTGA TCGCGCAAGT GCATGGTTGG TGCGTCGGCG GGGCCAGCGA CTACGCGCTG TGTGCCGACA TTGTGATCGC CAGCGAGGAC GCCGTGATCG GGACCCCGTA TAGCCGCATG TGGGGAGCCT ATTTGACCGG GATGTGGCTG TATCGACTCA GCCTTGCCAA GGTCAAATGG CACTCGCTGA CGGGCCGGCC GCTGACCGGT GTGCAGGCCG CCGAAGCCGA GCTGATCAAC GAGGCGGTGC CGTTCGAGCG GCTCGAGGCT CGCGTCGCCG AGATCGCCAC CGAGCTGGCA CGAATCCCGT TGTCACAGTT GCAAGCCCAG AAACTGATCG TCAACCAGGC CTACGAGAAC ATGGGCCTGG CCTCCACCCA GCTGCTGGGC GGCATTCTCG ACGGGCTGAT GCGCAACACC CCCGACGCGC TCGAGTTCAT CCGGACCGCC CAAACCCAGG GTGTGCGAGC CGCGGTCGAG CGCCGCGACG GCCCGTTCGG CGACTACAGC CAAGCCCCAC CGGAACTGCG ACCCGACCCC ACGCACGTCA TCACTCCTGA TGGGAGCATG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA