SSGCID: Target Page

MyboA.00358.b

PROBABLE ENOYL-COA HYDRATASE ECHA18 (ENOYL HYDRASE) (UNSATURATED ACYL-COA HYDRATASE) (CROTONASE)

CENTER ID:	MyboA.00358.b
ORGANISM:	Mycobacterium bovis AF2122/97 / ATCC BAA-935
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyboA.00358.b.A1.GE30557	Full length( MyboA.00358.b )		1	296

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyboA.00358.b.A1.PS00925	Full length( MyboA.00358.b )		1	296

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|233413.5.peg.3744
RefSeq:	NP_857049.1
UniProt:	A0A0H3P7Q0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRRAMTKMD EASNPCGGDI EAEMCQLMRE QPPAEGVVDR VALQRHRNVA LITLSHPQAQ NALNLASWRR LKRLLDDLAG ESGLRAVVLR GAGDKAFAAG ADIKEFPNTR MSAADAAEYN ESLAVCLRAL TTMPIPVIAA VRGLAVGGGC ELATACDVCI ATDDARFGIP LGKLGVTTGF TEADTVARLI GPAALKYLLF SGELIGIEEA ARWGLVQKVV APQDLAAATA KLVGQVCRQS AVTMRAAKVV ANMHGRALTG ADTDALIRFG VEAYEGADLR EGVAAFSQGR PPKFDD

NT Sequence

ATGAGGCGGC GTGCAATGAC GAAGATGGAC GAGGCTAGCA ATCCGTGCGG CGGGGACATC GAAGCTGAGA TGTGCCAGTT GATGCGCGAG CAACCACCCG CCGAAGGCGT CGTCGATCGT GTCGCGCTGC AACGCCATCG AAACGTTGCG TTGATCACGC TGAGCCATCC GCAGGCGCAG AACGCACTCA ACCTGGCGAG CTGGCGTCGG CTGAAGCGGC TGCTGGACGA TCTCGCCGGC GAATCGGGGC TGCGGGCGGT GGTGCTGCGG GGCGCCGGTG ACAAGGCGTT CGCCGCGGGT GCCGACATCA AGGAGTTTCC GAACACCCGC ATGAGCGCCG CGGACGCCGC GGAGTACAAC GAGAGCCTGG CCGTCTGCCT GAGGGCGTTG ACCACGATGC CGATCCCAGT CATCGCGGCG GTCCGGGGGC TCGCCGTCGG TGGCGGCTGT GAGCTGGCGA CGGCCTGCGA TGTGTGCATC GCGACCGACG ACGCGCGCTT CGGCATCCCG CTGGGCAAGC TCGGCGTCAC GACGGGCTTC ACCGAGGCGG ACACCGTCGC GCGCCTCATC GGTCCGGCGG CGCTGAAGTA TCTGTTGTTC AGCGGAGAAC TGATCGGCAT TGAGGAAGCC GCCCGCTGGG GATTGGTGCA AAAGGTCGTC GCACCACAGG ATTTGGCGGC CGCGACGGCC AAACTGGTCG GCCAGGTCTG TCGGCAATCC GCGGTGACCA TGCGTGCGGC GAAGGTGGTC GCCAACATGC ACGGCCGAGC GCTGACCGGC GCCGACACCG ATGCGCTGAT CCGGTTCGGT GTCGAAGCCT ACGAGGGGGC GGACCTACGC GAAGGGGTGG CGGCCTTCAG CCAGGGACGC CCACCCAAAT TTGATGAT

Details for MyboA.00358.b.A1.GE30557

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMRRRAMT KMDEASNPCG GDIEAEMCQL MREQPPAEGV VDRVALQRHR NVALITLSHP QAQNALNLAS WRRLKRLLDD LAGESGLRAV VLRGAGDKAF AAGADIKEFP NTRMSAADAA EYNESLAVCL RALTTMPIPV IAAVRGLAVG GGCELATACD VCIATDDARF GIPLGKLGVT TGFTEADTVA RLIGPAALKY LLFSGELIGI EEAARWGLVQ KVVAPQDLAA ATAKLVGQVC RQSAVTMRAA KVVANMHGRA LTGADTDALI RFGVEAYEGA DLREGVAAFS QGRPPKF

Validated NT Sequence

caaatttggg tgggcgtccc tggctgaagg ccgccacccc ttcgcgtagg tccgccccct cgtaggcttc gacaccgaac cggatcagcg catcggtgtc ggcgccggtc agcgctcggc cgtgcatgtt ggcgaccacc ttcgccgcac gcatggtcac cgcggattgc cgacagacct ggccgaccag tttggccgtc gcggccgcca aatcctgtgg tgcgacgacc ttttgcacca atccccagcg ggcggcttcc tcaatgccga tcagttctcc gctgaacaac agatacttca gcgccgccgg accgatgagg cgcgcgacgg tgtccgcctc ggtgaagccc gtcgtgacgc cgagcttgcc cagcgggatg ccgaagcgcg cgtcgtcggt cgcgatgcac acatcgcagg ccgtcgccag ctcacagccg ccaccgacgg cgagcccccg gaccgccgcg atgactggga tcggcatcgt ggtcaacgcc ctcaggcaga cggccaggct ctcgttgtac tccgcggcgt ccgcggcgct catgcgggtg ttcggaaact ccttgatgtc ggcacccgcg gcgaacgcct tgtcaccggc gccccgcagc accaccgccc gcagccccga ttcgccggcg agatcgtcca gcagccgctt cagccgacgc cagctcgcca ggttgagtgc gttctgcgcc tgcggatggc tcagcgtgat caacgcaacg tttcgatggc gttgcagcgc gacacgatcg acgacgcctt cggcgggtgg ttgctcgcgc atcaactggc acatctcagc ttcgatgtcc ccgccgcacg gattgctagc ctcgtccatc ttcgtcattg cacgccgcct catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRRRAMTKM DEASNPCGGD IEAEMCQLMR EQPPAEGVVD RVALQRHRNV ALITLSHPQA QNALNLASWR RLKRLLDDLA GESGLRAVVL RGAGDKAFAA GADIKEFPNT RMSAADAAEY NESLAVCLRA LTTMPIPVIA AVRGLAVGGG CELATACDVC IATDDARFGI PLGKLGVTTG FTEADTVARL IGPAALKYLL FSGELIGIEE AARWGLVQKV VAPQDLAAAT AKLVGQVCRQ SAVTMRAAKV VANMHGRALT GADTDALIRF GVEAYEGADL REGVAAFSQG RPPKFDD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaggcggcgt gcaatgacga agatggacga ggctagcaat ccgtgcggcg gggacatcga agctgagatg tgccagttga tgcgcgagca accacccgcc gaaggcgtcg tcgatcgtgt cgcgctgcaa cgccatcgaa acgttgcgtt gatcacgctg agccatccgc aggcgcagaa cgcactcaac ctggcgagct ggcgtcggct gaagcggctg ctggacgatc tcgccggcga atcggggctg cgggcggtgg tgctgcgggg cgccggtgac aaggcgttcg ccgcgggtgc cgacatcaag gagtttccga acacccgcat gagcgccgcg gacgccgcgg agtacaacga gagcctggcc gtctgcctga gggcgttgac cacgatgccg atcccagtca tcgcggcggt ccgggggctc gccgtcggtg gcggctgtga gctggcgacg gcctgcgatg tgtgcatcgc gaccgacgac gcgcgcttcg gcatcccgct gggcaagctc ggcgtcacga cgggcttcac cgaggcggac accgtcgcgc gcctcatcgg tccggcggcg ctgaagtatc tgttgttcag cggagaactg atcggcattg aggaagccgc ccgctgggga ttggtgcaaa aggtcgtcgc accacaggat ttggcggccg cgacggccaa actggtcggc caggtctgtc ggcaatccgc ggtgaccatg cgtgcggcga aggtggtcgc caacatgcac ggccgagcgc tgaccggcgc cgacaccgat gcgctgatcc ggttcggtgt cgaagcctac gagggggcgg acctacgcga aggggtggcg gccttcagcc agggacgccc acccaaattt gatgattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MyboA.00358.b.A1.PS00925

PURIFICATION DATe:	1/7/2011
CONCENTRATION:	69.72mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGGCGGCGT GCAATGACGA AGATGGACGA GGCTAGCAAT CCGTGCGGCG GGGACATCGA AGCTGAGATG TGCCAGTTGA TGCGCGAGCA ACCACCCGCC GAAGGCGTCG TCGATCGTGT CGCGCTGCAA CGCCATCGAA ACGTTGCGTT GATCACGCTG AGCCATCCGC AGGCGCAGAA CGCACTCAAC CTGGCGAGCT GGCGTCGGCT GAAGCGGCTG CTGGACGATC TCGCCGGCGA ATCGGGGCTG CGGGCGGTGG TGCTGCGGGG CGCCGGTGAC AAGGCGTTCG CCGCGGGTGC CGACATCAAG GAGTTTCCGA ACACCCGCAT GAGCGCCGCG GACGCCGCGG AGTACAACGA GAGCCTGGCC GTCTGCCTGA GGGCGTTGAC CACGATGCCG ATCCCAGTCA TCGCGGCGGT CCGGGGGCTC GCCGTCGGTG GCGGCTGTGA GCTGGCGACG GCCTGCGATG TGTGCATCGC GACCGACGAC GCGCGCTTCG GCATCCCGCT GGGCAAGCTC GGCGTCACGA CGGGCTTCAC CGAGGCGGAC ACCGTCGCGC GCCTCATCGG TCCGGCGGCG CTGAAGTATC TGTTGTTCAG CGGAGAACTG ATCGGCATTG AGGAAGCCGC CCGCTGGGGA TTGGTGCAAA AGGTCGTCGC ACCACAGGAT TTGGCGGCCG CGACGGCCAA ACTGGTCGGC CAGGTCTGTC GGCAATCCGC GGTGACCATG CGTGCGGCGA AGGTGGTCGC CAACATGCAC GGCCGAGCGC TGACCGGCGC CGACACCGAT GCGCTGATCC GGTTCGGTGT CGAAGCCTAC GAGGGGGCGG ACCTACGCGA AGGGGTGGCG GCCTTCAGCC AGGGACGCCC ACCCAAATTT GATGATTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA