MyavA.20433.a

Diacylglycerol acyltransferase/mycolyltransferase Ag85B (FbpB)

CENTER ID: MyavA.20433.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyavA.20433.a.AE1.GE44122 full length 1 330
MyavA.20433.a.HZ2.GE44128 mature protein 35 330
MyavA.20433.a.MB1.GE44123 full length 1 330
MyavA.20433.a.MB2.GE44127 mature protein 35 330
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: Q06947

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDLSEKVRA WGRRLLVGAA AAVTLPGLIG LAGGAATANA FSRPGLPVEY LQVPSAGMGR DIKVQFQSGG NGSPAVYLLD GLRAQDDYNG WDINTPAFEW YYQSGLSVIM PVGGQSSFYA DWYQPACGKA GCSTYKWETF LTSELPSYLA SNKGVKRTGN AAVGISMSGS SAMILAVNHP DQFIYAGSLS ALLDPSQGMG PSLIGLAMGD AGGYKADAMW GPSSDPAWQR NDPSLHIPEL VGHNTRLWLY CGNGTPSELG GANMPAEFLE NFVRSSNLKF QDAYNGAGGH NAVFNFNANG THSWEYWGAQ LNAMKPDLQG TLGASPGGGG
NT Sequence
atgacagatc tgagcgagaa ggtccgggcc tgggggcgcc ggcttttggt cggcgcagcg gcggctgtaa ccctgccggg cctgatcggt cttgccggcg gcgcggcgac cgcgaatgcg ttttcgcgtc cgggcctgcc cgtcgagtac ctgcaggtgc cctccgccgg aatgggccgc gacatcaagg tccagttcca gagcggcggc aacggctccc ccgcggtgta tctgctggac ggcctgcggg ctcaggacga ctacaacggc tgggacatca acaccccggc cttcgagtgg tactaccagt ccggcctgtc ggtgatcatg cccgtcggcg gacagtccag cttctacgcg gactggtacc agcccgcgtg cggcaaggcc ggttgctcca cctataagtg ggagaccttc ctgaccagcg agctgccgtc gtacctggcc tccaacaagg gtgtgaagcg caccggcaac gccgcagtcg gcatctcgat gtccggatcc tcggcgatga tcctggccgt caaccatccc gaccaattca tctatgccgg atcgctctcg gccctgctcg acccgtccca gggcatgggg ccgtcgctga tcggtctggc gatgggtgac gccggcggct acaaggccga cgccatgtgg ggcccgtcca gcgacccggc ctggcagcgc aacgacccga gcctgcacat ccccgagctg gtcggccaca acacccggct gtggctgtac tgcggtaacg ggacaccgtc ggagctgggt ggcgccaaca tgcccgccga gttcctggag aacttcgtgc gcagcagcaa cctgaagttc caggacgcct acaacggcgc cggcggccac aacgccgtgt tcaacttcaa cgccaacgga acgcacagct gggagtactg gggagcccag ctcaacgcca tgaagcccga cctgcagggc accctgggcg cgtccccggg cggcggcgga taa
Details for MyavA.20433.a.AE1.GE44122
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 24
PERCENT COVERAGE: 74
Validated AA Sequence
MXPRHQGPVP XRRQRXPRGV XAGXXRAQDD YNGWDINTPX FEWYYQSXLS VIMXVGGQSS XYADWYQPAC XKAGCSTYKW ETFLTSELPS YLASNKGVKR TGSAAVGISM SGSSAMILAV NHPDQFIYAG SLSALLDPSQ GMGPSLIGLA MGDAGGYKAD AMWGPSSDPA WQRNDPSLHI PELVGHNTRL WVYCGNGTPS ELGGANMPAE FLENFVRSSN LKFQDAYNGA GGHNAVFNFN ANGTHSWEYW GAQLNAMKPD LQGTLGASPG GGGAGHHHHH H
Validated NT Sequence
ttcnnnnnng gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaanc ttgncgacgg agctcgaatt cggatccgtt aatgatgatg atggtggtgc ccagctccgc cgccgcccgg ggacgcgccc agggtgccct gcaggtcggg cttcatggcg ttgagctggg ctccccagta ctcccagctg tgcgttccgt tggcgttgaa gttgaacacg gcgttgtggc cgccggcgcc gttgtaggcg tcctggaact tcaggttgct gctgcgcacg aagttctcca ggaactcggc gggcatgttg gcgccaccca gctccgacgg tgtcccgtta ccgcagtaca cccacagccg ggtgttgtgg ccgaccagct cggggatgtg caggctcggg tcgttgcgct gccaggccgg gtcgctggac gggccccaca tggcgtcggc cttgtagccg ccggcgtcac ccatcgccag accgatcagc gacggcccca tgccctggga cgggtcgagc agcgccgaga gcgatccggc atagatgaat tggtcgggat ggttgacggc caggatcatc gccgaggatc cggacatcga gatgccgacc gcggcgctgc cggtgcgctt cacacccttg ttcgaggcca ggtacgacgg cagctcgctg gtcaggaagg tctcccactt ataggtggag caaccggcct tgncgcacgc gggctggtac cagtccgcgt anaagctgga ctgtccgccg acnggcatga tcaccgacag gncggactgg tagtaccact cgaaggcggg gtgttgatgt cccagccgtt gtagtcgtcc tgagcccgca nncgtccagc anatacaccg cgggggancc gttgccgccg ctntggaact ggaccttgat gtcgcggccn cattcnngcg gagggnacct gcangtactc gacgggcagg cncnggangn naaaacnnaa ttcgcgggtc ncncncnncn gnagacnatc agnccngnna ggnnacannn ncnnngnncn aacnaaannc gngcangnnc nngaacttnn nnnnt
Expected Protein Sequence
MTDLSEKVRA WGRRLLVGAA AAVTLPGLIG LAGGAATANA FSRPGLPVEY LQVPSAGMGR DIKVQFQSGG NGSPAVYLLD GLRAQDDYNG WDINTPAFEW YYQSGLSVIM PVGGQSSFYA DWYQPACGKA GCSTYKWETF LTSELPSYLA SNKGVKRTGN AAVGISMSGS SAMILAVNHP DQFIYAGSLS ALLDPSQGMG PSLIGLAMGD AGGYKADAMW GPSSDPAWQR NDPSLHIPEL VGHNTRLWLY CGNGTPSELG GANMPAEFLE NFVRSSNLKF QDAYNGAGGH NAVFNFNANG THSWEYWGAQ LNAMKPDLQG TLGASPGGGG GHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gacagatctg agcgagaagg tccgggcctg ggggcgccgg cttttggtcg gcgcagcggc ggctgtaacc ctgccgggcc tgatcggtct tgccggcggc gcggcgaccg cgaatgcgtt ttcgcgtccg ggcctgcccg tcgagtacct gcaggtgccc tccgccggaa tgggccgcga catcaaggtc cagttccaga gcggcggcaa cggctccccc gcggtgtatc tgctggacgg cctgcgggct caggacgact acaacggctg ggacatcaac accccggcct tcgagtggta ctaccagtcc ggcctgtcgg tgatcatgcc cgtcggcgga cagtccagct tctacgcgga ctggtaccag cccgcgtgcg gcaaggccgg ttgctccacc tataagtggg agaccttcct gaccagcgag ctgccgtcgt acctggcctc caacaagggt gtgaagcgca ccggcaacgc cgcagtcggc atctcgatgt ccggatcctc ggcgatgatc ctggccgtca accatcccga ccaattcatc tatgccggat cgctctcggc cctgctcgac ccgtcccagg gcatggggcc gtcgctgatc ggtctggcga tgggtgacgc cggcggctac aaggccgacg ccatgtgggg cccgtccagc gacccggcct ggcagcgcaa cgacccgagc ctgcacatcc ccgagctggt cggccacaac acccggctgt ggctgtactg cggtaacggg acaccgtcgg agctgggtgg cgccaacatg cccgccgagt tcctggagaa cttcgtgcgc agcagcaacc tgaagttcca ggacgcctac aacggcgccg gcggccacaa cgccgtgttc aacttcaacg ccaacggaac gcacagctgg gagtactggg gagcccagct caacgccatg aagcccgacc tgcagggcac cctgggcgcg tccccgggcg gcggcggagg gcaccaccat catcatcatt aacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat
Details for MyavA.20433.a.HZ2.GE44128
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSAA TANAFSRPGL PVEYLQVPSA GMGRDIKVQF QSGGNGSPAV YLLDGLRAQD DYNGWDINTP AFEWYYQSGL SVIMPVGGQS SFYADWYQPA CGKAGCSTYK WETFLTSELP SYLASNKGVK RTGNAAVGIS MSGSSAMILA VNHPDQFIYA GSLSALLDPS QGMGPSLIGL AMGDAGGYKA DAMWGPSSDP AWQRNDPSLH IPELVGHNTR LWLYCGNGTP SELGGANMPA EFLENFVRSS NLKFQDAYNG AGGHNAVFNF NANGTHSWEY WGAQLNAMKP DLQGTLGASP GGGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg cggcgaccgc gaatgcgttt tcgcgtccgg gcctgcccgt cgagtacctg caggtgccct ccgccggaat gggccgcgac atcaaggtcc agttccagag cggcggcaac ggctcccccg cggtgtatct gctggacggc ctgcgggctc aggacgacta caacggctgg gacatcaaca ccccggcctt cgagtggtac taccagtccg gcctgtcggt gatcatgccc gtcggcggac agtccagctt ctacgcggac tggtaccagc ccgcgtgcgg caaggccggt tgctccacct ataagtggga gaccttcctg accagcgagc tgccgtcgta cctggcctcc aacaagggtg tgaagcgcac cggcaacgcc gcagtcggca tctcgatgtc cggatcctcg gcgatgatcc tggccgtcaa ccatcccgac caattcatct atgccggatc gctctcggcc ctgctcgacc cgtcccaggg catggggccg tcgctgatcg gtctggcgat gggtgacgcc ggcggctaca aggccgacgc catgtggggc ccgtccagcg acccggcctg gcagcgcaac gacccgagcc tgcacatccc cgagctggtc ggccacaaca cccggctgtg gctgtactgc ggtaacggga caccgtcgga gctgggtggc gccaacatgc ccgccgagtt cctggagaac ttcgtgcgca gcagcaacct gaagttccag gacgcctaca acggcgccgg cggccacaac gccgtgttca acttcaacgc caacggaacg cacagctggg agtactgggg agcccagctc aacgccatga agcccgacct gcagggcacc ctgggcgcgt ccccgggcgg cggcggataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyavA.20433.a.MB1.GE44123
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 80kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 28
Validated AA Sequence
YADWYQPACG KXGCSTYKWE XFLTSELPSY XASNKGVKRT GSAAVGISMS GSSAMILAVN HPDQFIYAGS LSALLDPSQG MGPSLIGLAM GDAGGYKADA MWGPSSDPAW QRNDPSLHIP ELVGHNTRLW VYCGNGTPSE LGGANMPAEF LENFVRSSNL KFQDAYNGAG GHNAVFNFNA NGTHSWEYWG AQLNAMKPDL QGTLGASPGG XG
Validated NT Sequence
gngnnnngtt agcagncgna tcctcgagaa ncttggctgc agaacttgtt cgtgctctnn nnnatccncc gccgcccggg gacgcgccca gggtgccctg caggtcgggc ttcatggcgt tgagctgggc tccccagtac tcccagctgt gcgttccgtt ggcgttgaag ttgaacacgg cgttgtggcc gccggcgccg ttgtaggcgt cctggaactt caggttgctg ctgcgcacga agttctccag gaactcggcg ggcatgttgg cgccacccag ctccgacggt gtcccgttac cgcagtacac ccacagccgg gtgttgtggc cgaccagctc ggggatgtgc aggctcgggt cgttgcgctg ccaggccggg tcgctggacg ggccccacat ggcgtcggcc ttgtagccgc cggcgtcacc catcgccaga ccgatcagcg acggccccat gccctgggac gggtcgagca gcgccgagag cgatccggca tagatgaatt ggtcgggatg gttgacggcc aggatcatcg ccgaggatcc ggacatcgag atgccgaccg cggcgctgcc ggtgcgcttc acacccttgt tcgaggccan gtacgacggc agctcgctgg tcaggaangt ctcccactta taggtggagc aaccggnctt gccgcacgcg ggctggtacc agtccgcgta naaagctgga ctgtccgccg acggggcatg atcnccgaca ggccggactg gtagtaccac tcnaaggccg gggtgttgat gtcccagccg ttgtantcgt cctgagcccg canccgtcca gcagatacnc cgcgggggag ccgttgnccg ccgcttctgg aactgnnacn ttnnatgtcg cggccncatt ccggcgggag ggcacctgcn ggtncttcga cgggcaggcc ncngacncnn aaacgcnttc gccggtccgc cgcgccnncn ggcaagaacn natnagnccc nncaggatta cnnnnccgnn gnngcnnnnn aaancnngnn ncnncanncn ngnancttnn nnntnnnnnt nnntcnnanc caggancccn tngncnnnnn nttcnnngna ncnncnnnan ncttnccnan gnnnnnnnnn anntnnnnt
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MTDLSEKVRA WGRRLLVGAA AAVTLPGLIG LAGGAATANA FSRPGLPVEY LQVPSAGMGR DIKVQFQSGG NGSPAVYLLD GLRAQDDYNG WDINTPAFEW YYQSGLSVIM PVGGQSSFYA DWYQPACGKA GCSTYKWETF LTSELPSYLA SNKGVKRTGN AAVGISMSGS SAMILAVNHP DQFIYAGSLS ALLDPSQGMG PSLIGLAMGD AGGYKADAMW GPSSDPAWQR NDPSLHIPEL VGHNTRLWLY CGNGTPSELG GANMPAEFLE NFVRSSNLKF QDAYNGAGGH NAVFNFNANG THSWEYWGAQ LNAMKPDLQG TLGASPGGGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgac agatctgagc gagaaggtcc gggcctgggg gcgccggctt ttggtcggcg cagcggcggc tgtaaccctg ccgggcctga tcggtcttgc cggcggcgcg gcgaccgcga atgcgttttc gcgtccgggc ctgcccgtcg agtacctgca ggtgccctcc gccggaatgg gccgcgacat caaggtccag ttccagagcg gcggcaacgg ctcccccgcg gtgtatctgc tggacggcct gcgggctcag gacgactaca acggctggga catcaacacc ccggccttcg agtggtacta ccagtccggc ctgtcggtga tcatgcccgt cggcggacag tccagcttct acgcggactg gtaccagccc gcgtgcggca aggccggttg ctccacctat aagtgggaga ccttcctgac cagcgagctg ccgtcgtacc tggcctccaa caagggtgtg aagcgcaccg gcaacgccgc agtcggcatc tcgatgtccg gatcctcggc gatgatcctg gccgtcaacc atcccgacca attcatctat gccggatcgc tctcggccct gctcgacccg tcccagggca tggggccgtc gctgatcggt ctggcgatgg gtgacgccgg cggctacaag gccgacgcca tgtggggccc gtccagcgac ccggcctggc agcgcaacga cccgagcctg cacatccccg agctggtcgg ccacaacacc cggctgtggc tgtactgcgg taacgggaca ccgtcggagc tgggtggcgc caacatgccc gccgagttcc tggagaactt cgtgcgcagc agcaacctga agttccagga cgcctacaac ggcgccggcg gccacaacgc cgtgttcaac ttcaacgcca acggaacgca cagctgggag tactggggag cccagctcaa cgccatgaag cccgacctgc agggcaccct gggcgcgtcc ccgggcggcg gcggataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyavA.20433.a.MB2.GE44127
HARVESTED ON: 3/2/2021
SEQUENCED ON: 3/10/2021
EXPECTED MW: 76kDa
OBSERVED MW: 76kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS AATANAFSRP GLPVEYLQVP SAGMGRDIKV QFQSGGNGSP AVYLLDGLRA QDDYNGWDIN TPAFEWYYQS GLSVIMPVGG QSSFYADWYQ PACGKAGCST YKWETFLTSE LPSYLASNKG VKRTGNAAVG ISMSGSSAMI LAVNHPDQFI YAGSLSALLD PSQGMGPSLI GLAMGDAGGY KADAMWGPSS DPAWQRNDPS LHIPELVGHN TRLWLYCGNG TPSELGGANM PAEFLENFVR SSNLKFQDAY NGAGGHNAVF NFNANGTHSW EYWGAQLNAM KPDLQGTLGA SPGGGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaacaac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcggcggc gaccgcgaat gcgttttcgc gtccgggcct gcccgtcgag tacctgcagg tgccctccgc cggaatgggc cgcgacatca aggtccagtt ccagagcggc ggcaacggct cccccgcggt gtatctgctg gacggcctgc gggctcagga cgactacaac ggctgggaca tcaacacccc ggccttcgag tggtactacc agtccggcct gtcggtgatc atgcccgtcg gcggacagtc cagcttctac gcggactggt accagcccgc gtgcggcaag gccggttgct ccacctataa gtgggagacc ttcctgacca gcgagctgcc gtcgtacctg gcctccaaca agggtgtgaa gcgcaccggc aacgccgcag tcggcatctc gatgtccgga tcctcggcga tgatcctggc cgtcaaccat cccgaccaat tcatctatgc cggatcgctc tcggccctgc tcgacccgtc ccagggcatg gggccgtcgc tgatcggtct ggcgatgggt gacgccggcg gctacaaggc cgacgccatg tggggcccgt ccagcgaccc ggcctggcag cgcaacgacc cgagcctgca catccccgag ctggtcggcc acaacacccg gctgtggctg tactgcggta acgggacacc gtcggagctg ggtggcgcca acatgcccgc cgagttcctg gagaacttcg tgcgcagcag caacctgaag ttccaggacg cctacaacgg cgccggcggc cacaacgccg tgttcaactt caacgccaac ggaacgcaca gctgggagta ctggggagcc cagctcaacg ccatgaagcc cgacctgcag ggcaccctgg gcgcgtcccc gggcggcggc ggataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa