MyavA.19465.a

Dephospho-CoA kinase CoaE (EC 2.7.1.24)

CENTER ID:	MyavA.19465.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	native diffraction-data
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.19465.a.B1.GE40746	full length		1	407
MyavA.19465.a.B2.GE40747	nterm domain		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.19465.a.B1.PW37998	full length		1	407

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_81926

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0H2ZYM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRIGLTGGI GAGKSALSSA FAQCGAVIVD GDVIAREVVR PGTEGLAALV EAFGRDILLA DGSLDRPALA AKAFADDAAR QTLNGIVHPL VGARRAEIIA SVPADSVVVE DIPLLVESGM APLFPLVVIV YADVEVRLRR LVEQRGMAEA DARARIAAQA SDEQRRAVAD IWLDNSGSPA ELVQRAQQVW NERIVPFAHN LSTRQIARAP VRLVPPDPEW PAQAQRIVNR LKTASGHRAL RVDHVGSTAL PGDPDFAAKD VIDIQITVES LAAADELVEP LLAAGYPRLE HITADVAKPD ARSTVERYDH TDDPALWHKR IHASADPGRP TNVHIRVDGW PGQQFALLFV DWLTADPDAR ADYLAVKRSA EQQADGDIDA YVAVKEPWFR DAYRRAWDWA DSTGWKP

NT Sequence

atgctgcgca tcgggttgac cggcggcatc ggcgccggca agtcggcgct gtcctccgcg ttcgcgcagt gcggtgcggt catcgtcgac ggtgacgtca tcgcccgtga ggtggtccgg cccggtaccg agggcctggc ggcgctggtc gaggcgttcg gacgcgacat cctgctcgcg gacggatcgc tggaccggcc ggcgttggcg gccaaggctt tcgccgacga cgccgctcgt cagacgctca acgggatcgt gcatccgctg gtgggtgcgc gccgcgccga gataatcgcg tcggtgcccg cggattccgt tgtggtcgag gacattccgc tgctggtgga atccggtatg gcgccgctgt tcccgctcgt cgtcatcgtg tacgccgatg tcgaggtgcg gctgcggcgg ctggtcgagc agcgcggcat ggccgaagcc gacgcccgcg cccggatcgc cgctcaggcc agcgacgagc agcgccgcgc cgtcgccgac atctggctgg acaactcggg cagcccggcg gagttggtgc aacgggccca gcaggtgtgg aacgagcgca tcgtgccgtt cgcccacaac ctgagcacgc gtcagatcgc ccgggcgccg gtgcggttgg tgccgcccga tcccgagtgg ccggcccagg cgcagcgaat cgtcaaccgg ctcaagaccg catccggcca tcgggcgctg cgcgtcgacc acgtcggctc cacggcgttg cccggggatc cggatttcgc ggccaaggac gtcatcgaca tccagatcac cgtggaatcc ctggccgccg ccgacgaact cgtcgaaccg ttgctggccg ccggataccc tcgcctcgaa cacatcaccg ccgacgtcgc caagcccgac gcccgcagca ccgtcgagcg ctacgatcac accgacgatc cggccttgtg gcacaagcgg attcacgcct cggcggaccc gggccggccc accaacgtgc acatccgggt ggacggctgg cccggccagc agttcgccct gctgttcgtg gactggttga ccgccgaccc cgatgctcgg gcggactatc tggccgtcaa gcgcagcgcc gaacagcagg ccgacggtga catcgacgcc tacgtcgccg tcaaggagcc gtggtttcgc gacgcctacc ggcgggcctg ggattgggcc gactcgacgg gctggaaacc ctag

Details for MyavA.19465.a.B1.GE40746

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	45kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TAGLAALVEA FGSDILLADG SLDRPALAAK AFADDEARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TRQIARAPVR LVPPDPEWPA QAQRIVNRLK TASGHRALRV DHVGSTALPG DPDFAAKDVI DIQITVESLA AADELVEPLL AAGYPRLEHI TADVAKPDAR STVERYDHTD DPALWHKRIH ASADPGRPTN VHIRVDGWPG QQFALLFVDW LTADPDARAD YLAVKRSAEQ QADGDIDAYV AVKEPWFRDA YRRAWDWADS TGWKP

Validated NT Sequence

atggctcacc accaccacca ccatatgctg cgcatcgggt tgaccggcgg catcggcgcc ggcaagtcgg cgctgtcctc cgcgttcgcg cagtgcggtg cggtcatcgt cgacggtgac gtcatcgccc gtgaggtggt ccggcccggt accgcgggcc tggcggcgct ggtcgaggcg ttcggcagcg acatcctgct cgccgacgga tcgctggacc ggccggcgtt ggcggccaag gctttcgccg acgacgaagc gcgccagacg ctcaacggga tcgtgcatcc gctggtgggt gcgcgccgcg ccgagataat cgcgtcggtg cccgcggatt ccgttgtggt cgaggacatt ccgctgctgg tggaatccgg gatggcgccg ctgttcccgc tcgtcgtcat cgtgtacgcc -gatgtcgag gtgcggctgc ggcggctggt cgagcagcgc ggcatggccg aagccgacgc ccgcgcccgg atcgccgctc aggccagcga cgagcagcgc cgcgccgtcg ccgacatctg gctggacaac tcgggcagcc cggcggagtt ggtgcaacgg gcccagcagg tgtggaacga gcgcatcgtg ccgttcgccc acaacctgag cacgcgtcag atcgcccggg cgccggtgcg gttggtgccg ccc-gatccc gagtggccgg cccaggcgca gcgaatcgtc aaccggctca agaccgcatc cggccatcgg gcgttgcgcg tcgaccacgt cggctccacg gcgttgcccg gggatccgga tttcgcggcc aaggacgtca tcgacatcca gatcaccgtg gaatccctgg ccgccgccga cgaactcgtc gaaccgttgc tggccgccgg ataccctcgc ctggaacaca tcaccgccga cgtcgccaag cccgacgccc gcagcaccgt cgagcgctac gatcacaccg acgatccggc cttgtggcac aagcggattc acgcctcggc ggacccgggc cggcccacca acgtgcacat ccgggtggac ggctggcccg gccagcagtt cgccctgctg ttcgtggact ggttgaccgc cgaccccgat gctcgggcgg actatctggc cgtcaagcgc agcgccgaac agcaggccga cggtgacatc gacgcctacg tcgccgtcaa ggagccgtgg tttcgcgacg cctaccggcg ggcctgggat tgggccgact cgacgggctg gaaaccctaa

Expected Protein Sequence

MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TEGLAALVEA FGRDILLADG SLDRPALAAK AFADDAARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TRQIARAPVR LVPPDPEWPA QAQRIVNRLK TASGHRALRV DHVGSTALPG DPDFAAKDVI DIQITVESLA AADELVEPLL AAGYPRLEHI TADVAKPDAR STVERYDHTD DPALWHKRIH ASADPGRPTN VHIRVDGWPG QQFALLFVDW LTADPDARAD YLAVKRSAEQ QADGDIDAYV AVKEPWFRDA YRRAWDWADS TGWKP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggtt gaccggcggc atcggcgccg gcaagtcggc gctgtcctcc gcgttcgcgc agtgcggtgc ggtcatcgtc gacggtgacg tcatcgcccg tgaggtggtc cggcccggta ccgagggcct ggcggcgctg gtcgaggcgt tcggacgcga catcctgctc gcggacggat cgctggaccg gccggcgttg gcggccaagg ctttcgccga cgacgccgct cgtcagacgc tcaacgggat cgtgcatccg ctggtgggtg cgcgccgcgc cgagataatc gcgtcggtgc ccgcggattc cgttgtggtc gaggacattc cgctgctggt ggaatccggt atggcgccgc tgttcccgct cgtcgtcatc gtgtacgccg atgtcgaggt gcggctgcgg cggctggtcg agcagcgcgg catggccgaa gccgacgccc gcgcccggat cgccgctcag gccagcgacg agcagcgccg cgccgtcgcc gacatctggc tggacaactc gggcagcccg gcggagttgg tgcaacgggc ccagcaggtg tggaacgagc gcatcgtgcc gttcgcccac aacctgagca cgcgtcagat cgcccgggcg ccggtgcggt tggtgccgcc cgatcccgag tggccggccc aggcgcagcg aatcgtcaac cggctcaaga ccgcatccgg ccatcgggcg ctgcgcgtcg accacgtcgg ctccacggcg ttgcccgggg atccggattt cgcggccaag gacgtcatcg acatccagat caccgtggaa tccctggccg ccgccgacga actcgtcgaa ccgttgctgg ccgccggata ccctcgcctc gaacacatca ccgccgacgt cgccaagccc gacgcccgca gcaccgtcga gcgctacgat cacaccgacg atccggcctt gtggcacaag cggattcacg cctcggcgga cccgggccgg cccaccaacg tgcacatccg ggtggacggc tggcccggcc agcagttcgc cctgctgttc gtggactggt tgaccgccga ccccgatgct cgggcggact atctggccgt caagcgcagc gccgaacagc aggccgacgg tgacatcgac gcctacgtcg ccgtcaagga gccgtggttt cgcgacgcct accggcgggc ctgggattgg gccgactcga cgggctggaa accctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for MyavA.19465.a.B2.GE40747

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggtt gaccggcggc atcggcgccg gcaagtcggc gctgtcctcc gcgttcgcgc agtgcggtgc ggtcatcgtc gacggtgacg tcatcgcccg tgaggtggtc cggcccggta ccgagggcct ggcggcgctg gtcgaggcgt tcggacgcga catcctgctc gcggacggat cgctggaccg gccggcgttg gcggccaagg ctttcgccga cgacgccgct cgtcagacgc tcaacgggat cgtgcatccg ctggtgggtg cgcgccgcgc cgagataatc gcgtcggtgc ccgcggattc cgttgtggtc gaggacattc cgctgctggt ggaatccggt atggcgccgc tgttcccgct cgtcgtcatc gtgtacgccg atgtcgaggt gcggctgcgg cggctggtcg agcagcgcgg catggccgaa gccgacgccc gcgcccggat cgccgctcag gccagcgacg agcagcgccg cgccgtcgcc gacatctggc tggacaactc gggcagcccg gcggagttgg tgcaacgggc ccagcaggtg tggaacgagc gcatcgtgcc gttcgcccac aacctgagca cgcgttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MyavA.19465.a.B1.PW37998

PURIFICATION DATe:	12/13/2016
CONCENTRATION:	28mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTGC GCATCGGGTT GACCGGCGGC ATCGGCGCCG GCAAGTCGGC GCTGTCCTCC GCGTTCGCGC AGTGCGGTGC GGTCATCGTC GACGGTGACG TCATCGCCCG TGAGGTGGTC CGGCCCGGTA CCGAGGGCCT GGCGGCGCTG GTCGAGGCGT TCGGACGCGA CATCCTGCTC GCGGACGGAT CGCTGGACCG GCCGGCGTTG GCGGCCAAGG CTTTCGCCGA CGACGCCGCT CGTCAGACGC TCAACGGGAT CGTGCATCCG CTGGTGGGTG CGCGCCGCGC CGAGATAATC GCGTCGGTGC CCGCGGATTC CGTTGTGGTC GAGGACATTC CGCTGCTGGT GGAATCCGGT ATGGCGCCGC TGTTCCCGCT CGTCGTCATC GTGTACGCCG ATGTCGAGGT GCGGCTGCGG CGGCTGGTCG AGCAGCGCGG CATGGCCGAA GCCGACGCCC GCGCCCGGAT CGCCGCTCAG GCCAGCGACG AGCAGCGCCG CGCCGTCGCC GACATCTGGC TGGACAACTC GGGCAGCCCG GCGGAGTTGG TGCAACGGGC CCAGCAGGTG TGGAACGAGC GCATCGTGCC GTTCGCCCAC AACCTGAGCA CGCGTCAGAT CGCCCGGGCG CCGGTGCGGT TGGTGCCGCC CGATCCCGAG TGGCCGGCCC AGGCGCAGCG AATCGTCAAC CGGCTCAAGA CCGCATCCGG CCATCGGGCG CTGCGCGTCG ACCACGTCGG CTCCACGGCG TTGCCCGGGG ATCCGGATTT CGCGGCCAAG GACGTCATCG ACATCCAGAT CACCGTGGAA TCCCTGGCCG CCGCCGACGA ACTCGTCGAA CCGTTGCTGG CCGCCGGATA CCCTCGCCTC GAACACATCA CCGCCGACGT CGCCAAGCCC GACGCCCGCA GCACCGTCGA GCGCTACGAT CACACCGACG ATCCGGCCTT GTGGCACAAG CGGATTCACG CCTCGGCGGA CCCGGGCCGG CCCACCAACG TGCACATCCG GGTGGACGGC TGGCCCGGCC AGCAGTTCGC CCTGCTGTTC GTGGACTGGT TGACCGCCGA CCCCGATGCT CGGGCGGACT ATCTGGCCGT CAAGCGCAGC GCCGAACAGC AGGCCGACGG TGACATCGAC GCCTACGTCG CCGTCAAGGA GCCGTGGTTT CGCGACGCCT ACCGGCGGGC CTGGGATTGG GCCGACTCGA CGGGCTGGAA ACCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT