MyavA.19336.a

Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX [Includes: Lysine--tRNA ligase (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS); Phosphatidylglycerol lysyltransferase (EC 2.3.2.3) (Lysylphosphatidylglycerol synthetase) (LPG synthetase)]

CENTER ID: MyavA.19336.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.19336.a.B2.GE40745 soluble domain 304 1177
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3059
UniProt: A0QHC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRAGRSRQF SSVEEAFSTS AARPGRRGRR SGRENTAKFV PATLSASTSF SRIEGISSRS VTLASPGSRS GSGPRSGPRL GPANRSTSRY RWVPAAAGWT VGVIATVSLL GSVSPLIRYL IKVPREFIND YLFNFPDTSI AWSFVLALLA AALTARKRIA WLLLLGNMIL AAALNVADIA AGDNTAAEIF GENLGFAVHI VAIVLLVLAY REFWAKVRKG ALVKAAAVLV AGDVVGILVS WGLVELFPGT LARQDRLPYV VNRVVGFALA DPDLFTGRPH VFLNAIFGLF GALALIMATI VLFQSQRAEN ALTGEDESAI RGLLELYGKN DSLGYFATRR DKSVVFAQSG RAAITYRVEI GVCLASGDPI GDPRAWPQAV DAWLGLCQTY GWAPGVMGAS TQGARTYREA GLNALELGDE AILRTSEFKL SGPDMRGVRQ AVTRARRAGL TVRIRRHRDI SPEAMADTIA RADAWRDTQT ERGFSMALGR LGDPADGDCL LVEAIDRDGS VVAMLSLVPW GTTGVSLDLM RRSPSSPNGT IELMVSELAL NAESLGITRI SLNFAMFRSA FEQGAQLGAG PVARLWRGLL LFFSRWWQLE TLYRSNMKYQ PDWVPRYACY EDARLIPRVG VASVIAEGFL VLPFSRRDKV HTGHHPAVPA RLAQSGLLHH DGSAPDVSGL RPERTDAEEA RSRLPEQVRV RLAKLKVLQR NGVDAYPVGC PPSHTVAQAL DADDQQDITV AGRILRIRDF GGVLFAQLRD WSGEMQVLLD NSRLERGRTA DFTAAIDLGD LVEVSGQMGF SKKGTRSLIV TDWRMIGKCL RPLPNKWKGL TDPEARVRTR YVDLAVNPES RELIAARSEV LRSVRQTLFA KGFIEVETPI LQQIHGGATA RPFVTHINTY DMDLFLRIAP ELYLKRLCVG GVERVFELGR AFRNEGVDFS HNPEFTLLEA YQAHADYRVW IDGCRELIQN AAQAAHGEQT VLRPGADGRL QPVDISGIWA VKTVHDAVSE ALGEQVDPGT SLSTLRKLSD AARIPYRAHW DAGAVVLELY EHLVEDRTEE PTFYVDFPTS VSPLTRPHRS RPGVAERWDL VAWGVELATA YSELTDPVEQ RRRLQEQSLL AAGGDPEAME LDEDFLQAME YAMPPTGGLG MGVDRLVMLI TGRSIRETLP FPLAKPH
NT Sequence
ttgcgccggg ctggtcgcag ccgtcagttt agcagtgttg aggaggcttt tagcacttcg gccgcccgac cgggccggcg cgggcgtcga tccggccggg aaaacaccgc gaaattcgtg ccggccactc ttagcgcttc gacaagcttt tcacggatag agggcatatc ctcgcggtct gtgacactcg catctcctgg ttcgaggtct ggttcgggtc ctcgttcggg gccgcggttg gggcctgcaa accggtcgac ctcgcggtat cgctgggtgc cggcggccgc cgggtggacc gtgggtgtga tcgccacggt gtcgctgctg ggcagcgtgt ccccgctgat ccggtacctg atcaaggtcc cgcgcgagtt catcaacgac tacctgttca acttccccga caccagcatc gcctggtcgt tcgtgctggc cctgctggcg gcggcgctga cggcacgcaa gcggatcgcc tggctgctgt tgctgggcaa catgatcctg gccgccgccc tcaacgtcgc cgacatcgcc gccggtgaca acaccgcggc cgagatcttc ggcgagaacc tgggtttcgc cgtccacatc gtcgcgatcg tcctgctggt gctggcgtat cgggagttct gggccaaggt ccgcaagggc gcgttggtca aggcggccgc ggtgctggtc gccggggacg tggtgggcat cctggtgtcg tggggcctgg tcgagttgtt ccccggcacc ctggcccgcc aggaccggct gccctacgtg gtcaaccggg tggtcggctt cgcgctggcc gacccggacc tgttcaccgg ccgcccgcac gtgttcctca acgcgatctt cgggttgttc ggcgcgctcg cgctgatcat ggcgaccatc gtgctctttc aatcccagcg cgcggagaac gcgctgaccg gtgaggacga gtcggccatc cgcggactgc tggagttgta cggcaagaac gactcgctgg gctacttcgc cacccgccgc gacaagtcgg tggtcttcgc ccagagcgga cgcgccgcca tcacctaccg ggtcgagatc ggggtctgtt tggccagcgg cgacccgatc ggcgatcccc gcgcctggcc gcaggcggtc gacgcctggc tgggcctgtg ccagacctac ggctgggcgc ccggcgtgat gggcgccagc acgcaggggg ctcggaccta tcgcgaggcc ggcctcaacg cgctcgagct gggtgacgag gccatcctgc gcacctccga gttcaagctg tccggcccgg acatgcgcgg cgtccgccag gcggtgaccc gggcccgccg cgccgggttg acggtgcgga tccgccggca ccgcgacatc tcccccgagg cgatggccga caccatcgcc cgcgccgacg cctggcgtga cacccagacc gagcgcggct tctccatggc gctgggccga ctcggtgacc cggccgacgg ggactgcctg ctggtggagg ccatcgaccg ggacggcagc gtggtcgcga tgctgtcgct ggtcccgtgg ggcaccaccg gtgtgtcgct ggacttgatg cgccggtcgc cgagttcacc caacggcacc atcgagctga tggtcagcga gctggcgttg aatgctgaaa gccttggtat cacccgcatt tcgctgaact tcgcgatgtt tcggtcggcg ttcgaacagg gcgcccagct gggcgccggc ccggtcgcgc ggctgtggcg ggggttgctg ctgttcttct cccggtggtg gcagctggag accctgtacc gctcgaacat gaagtaccaa cccgattggg ttccgcgcta cgcctgctac gaggatgccc ggctgatccc ccgggtcggc gtcgcgtcgg tgatcgccga gggcttcctg gtgttgccgt tcagccggcg cgacaaggtg cacaccggcc accaccccgc cgtgccggcc cggctggcgc agtcgggcct gctgcaccac gacggctcgg caccggacgt gagcgggctg cgcccggagc gcaccgacgc cgaggaggcg cgctcgcggc tgcccgagca ggttagggtg cgcctggcca agctgaaggt gttgcagcgc aacggtgttg acgcctatcc ggtgggctgt ccgcccagcc acaccgtcgc gcaggcgctg gacgccgacg accagcagga catcaccgtc gccggccgga tcctgcggat ccgcgacttc ggtggggtgc tgttcgccca gctgcgggac tggtcggggg aaatgcaagt gctgctagac aattcgcgtc tggagcgggg ccgcacggcc gacttcaccg cggccatcga tctcggcgac ctggtcgagg tgtccggtca gatggggttc tccaagaaag gcacccggtc gctgatcgtc accgactggc ggatgatcgg taagtgcttg cggccattgc ccaataagtg gaaggggctc accgatccgg aggcccgggt gcgaacccgc tacgtcgacc tggcggtcaa ccccgagtcg cgggagttga tcgcggcgcg cagcgaggtg ctgcgctcgg tccggcagac cctgttcgcc aagggattca tcgaggtcga gaccccgatc ctgcagcaga tccacggcgg cgccaccgcc cgaccgttcg tcacgcacat caacacctac gacatggacc tgttcctgcg catcgcgccg gagctgtatc tgaagcggct gtgcgtcggc ggcgtggagc gcgtcttcga gttgggccgg gcgttccgca acgagggggt cgacttcagc cacaacccgg aattcaccct gctggaggcc taccaggcgc acgccgacta tcgggtgtgg atcgacgggt gccgcgagct gatccagaac gccgcccagg ccgcgcacgg cgagcagacc gtgctgcggc ccggcgccga cggccggctg caaccggtcg acatctccgg gatctgggcg gtcaagaccg tgcacgacgc ggtgtccgag gcgctcggcg agcaggtcga ccccggcacg tcgctgtcca ccctgcgcaa actgtccgac gcggcgcgca tcccctaccg cgcgcactgg gacgccggcg cggtggtgct cgagctctac gagcacctgg tcgaggaccg gaccgaggag ccgacgttct acgtcgactt ccccacctct gtgtcaccgc tgacccggcc gcaccgcagc cggcccgggg tcgcggagcg gtgggacctg gtggcctggg gtgtcgaact ggccaccgcc tacagcgagc tgaccgatcc ggtggagcag cgccgccgcc tgcaggagca gtcgctgtta gctgccggcg gggatcccga ggcgatggaa ctcgacgagg acttcctgca ggcgatggag tacgcgatgc cgccgacggg cgggctgggc atgggcgtcg atcgcctcgt catgctcata acgggccgca gcatccgcga aaccttgccg tttccgctgg ccaagccgca ttag
Details for MyavA.19336.a.B2.GE40745
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 98kDa
OBSERVED MW: 99kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHQS QRAENALTGE DESAIRGLLE LYGKNDSLGY FATRRDKSVV FAQSGRAAIT YRVEIGVCLA SGDPIGDPRA WPQAVDAWLG LCQTYGWAPG VMGASTQGAR TYREAGLNAL ELGDEAILRT SEFKLSGPDM RGVRQAVTRA RRAGLTVRIR RHRDISPEAM ADTIARADAW RDTQTERGFS MALGRLGDPA DGDCLLVEAI DRDGSVVAML SLVPWGTTGV SLDLMRRSPS SPNGTIELMV SELALNAESL GITRISLNFA MFRSAFEQGA QLGAGPVARL WRGLLLFFSR WWQLETLYRS NMKYQPDWVP RYACYEDARL IPRVGVASVI AEGFLVLPFS RRDKVHTGHH PAVPARLAQS GLLHHDGSAP DVSGLRPERT DAEEARSRLP EQVRVRLAKL KVLQRNGVDA YPVGCPPSHT VAQALDADDQ QDITVAGRIL RIRDFGGVLF AQLRDWSGEM QVLLDNSRLE RGRTADFTAA IDLGDLVEVS GQMGFSKKGT RSLIVTDWRM IGKCLRPLPN KWKGLTDPEA RVRTRYVDLA VNPESRELIA ARSEVLRSVR QTLFAKGFIE VETPILQQIH GGATARPFVT HINTYDMDLF LRIAPELYLK RLCVGGVERV FELGRAFRNE GVDFSHNPEF TLLEAYQAHA DYRVWIDGCR ELIQNAAQAA HGEQTVLRPG ADGRLQPVDI SGIWAVKTVH DAVSEALGEQ VDPGTSLSTL RKLSDAARIP YRAHWDAGAV VLELYEHLVE DRTEEPTFYV DFPTSVSPLT RPHRSRPGVA ERWDLVAWGV ELATAYSELT DPVEQRRRLQ EQSLLAAGGD PEAMELDEDF LQAMEYAMPP TGGLGMGVDR LVMLITGRSI RETLPFPLAK PH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaatccc agcgcgcgga gaacgcgctg accggtgagg acgagtcggc catccgcgga ctgctggagt tgtacggcaa gaacgactcg ctgggctact tcgccacccg ccgcgacaag tcggtggtct tcgcccagag cggacgcgcc gccatcacct accgggtcga gatcggggtc tgtttggcca gcggcgaccc gatcggcgat ccccgcgcct ggccgcaggc ggtcgacgcc tggctgggcc tgtgccagac ctacggctgg gcgcccggcg tgatgggcgc cagcacgcag ggggctcgga cctatcgcga ggccggcctc aacgcgctcg agctgggtga cgaggccatc ctgcgcacct ccgagttcaa gctgtccggc ccggacatgc gcggcgtccg ccaggcggtg acccgggccc gccgcgccgg gttgacggtg cggatccgcc ggcaccgcga catctccccc gaggcgatgg ccgacaccat cgcccgcgcc gacgcctggc gtgacaccca gaccgagcgc ggcttctcca tggcgctggg ccgactcggt gacccggccg acggggactg cctgctggtg gaggccatcg accgggacgg cagcgtggtc gcgatgctgt cgctggtccc gtggggcacc accggtgtgt cgctggactt gatgcgccgg tcgccgagtt cacccaacgg caccatcgag ctgatggtca gcgagctggc gttgaatgct gaaagccttg gtatcacccg catttcgctg aacttcgcga tgtttcggtc ggcgttcgaa cagggcgccc agctgggcgc cggcccggtc gcgcggctgt ggcgggggtt gctgctgttc ttctcccggt ggtggcagct ggagaccctg taccgctcga acatgaagta ccaacccgat tgggttccgc gctacgcctg ctacgaggat gcccggctga tcccccgggt cggcgtcgcg tcggtgatcg ccgagggctt cctggtgttg ccgttcagcc ggcgcgacaa ggtgcacacc ggccaccacc ccgccgtgcc ggcccggctg gcgcagtcgg gcctgctgca ccacgacggc tcggcaccgg acgtgagcgg gctgcgcccg gagcgcaccg acgccgagga ggcgcgctcg cggctgcccg agcaggttag ggtgcgcctg gccaagctga aggtgttgca gcgcaacggt gttgacgcct atccggtggg ctgtccgccc agccacaccg tcgcgcaggc gctggacgcc gacgaccagc aggacatcac cgtcgccggc cggatcctgc ggatccgcga cttcggtggg gtgctgttcg cccagctgcg ggactggtcg ggggaaatgc aagtgctgct agacaattcg cgtctggagc ggggccgcac ggccgacttc accgcggcca tcgatctcgg cgacctggtc gaggtgtccg gtcagatggg gttctccaag aaaggcaccc ggtcgctgat cgtcaccgac tggcggatga tcggtaagtg cttgcggcca ttgcccaata agtggaaggg gctcaccgat ccggaggccc gggtgcgaac ccgctacgtc gacctggcgg tcaaccccga gtcgcgggag ttgatcgcgg cgcgcagcga ggtgctgcgc tcggtccggc agaccctgtt cgccaaggga ttcatcgagg tcgagacccc gatcctgcag cagatccacg gcggcgccac cgcccgaccg ttcgtcacgc acatcaacac ctacgacatg gacctgttcc tgcgcatcgc gccggagctg tatctgaagc ggctgtgcgt cggcggcgtg gagcgcgtct tcgagttggg ccgggcgttc cgcaacgagg gggtcgactt cagccacaac ccggaattca ccctgctgga ggcctaccag gcgcacgccg actatcgggt gtggatcgac gggtgccgcg agctgatcca gaacgccgcc caggccgcgc acggcgagca gaccgtgctg cggcccggcg ccgacggccg gctgcaaccg gtcgacatct ccgggatctg ggcggtcaag accgtgcacg acgcggtgtc cgaggcgctc ggcgagcagg tcgaccccgg cacgtcgctg tccaccctgc gcaaactgtc cgacgcggcg cgcatcccct accgcgcgca ctgggacgcc ggcgcggtgg tgctcgagct ctacgagcac ctggtcgagg accggaccga ggagccgacg ttctacgtcg acttccccac ctctgtgtca ccgctgaccc ggccgcaccg cagccggccc ggggtcgcgg agcggtggga cctggtggcc tggggtgtcg aactggccac cgcctacagc gagctgaccg atccggtgga gcagcgccgc cgcctgcagg agcagtcgct gttagctgcc ggcggggatc ccgaggcgat ggaactcgac gaggacttcc tgcaggcgat ggagtacgcg atgccgccga cgggcgggct gggcatgggc gtcgatcgcc tcgtcatgct cataacgggc cgcagcatcc gcgaaacctt gccgtttccg ctggccaagc cgcattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat