MyavA.19282.a

Polyphosphate kinase (EC 2.7.4.1) (ATP-polyphosphate phosphotransferase) (Polyphosphoric acid kinase)

CENTER ID: MyavA.19282.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.19282.a.B2.GE40288 ordered domain 46 730
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.19282.a.B2.PS38267 ordered domain 46 730

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3755
UniProt: A0QJB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMRHDRNVTE IDAETRPDEN LWHSGDSAVG APPAATPAAM TDLPEDRYLN RELSWLDFNA RVLALADDNS LPLLERAKFL AIFASNLDEF YMVRVAGLKR RDEMGLSVRS ADGLTPRKQL ALIGEHTQRI ATRHARVFLD SVRPALAEEG IHIVTWADLD QAERDELSTY FTEQVFPVLT PLAVDPAHPF PFVSGLSLNL AVMVRQTEDG GQHFARVKVP NNVDRFVELA APRAGAEGEN RGVVRFLPME ELIAAFLPLL FPGMEIVEHH AFRITRNADM EVEEDRDEDL LQALERELAR RRFGPPVRLE IADDMTEGML ELLLRELDVH PGDVIEVPGL LDLSSLRQIY DLDRPALKDP AFVPDTHPAF ADRESPKSIF ATLREGDVLV HHPYDSFSTS VQRFIQQAAA DPNVLAIKQT LYRTSGDSPI VRALIEAAEA GKQAVALVEI KARFDEQANI RWARALEQAG VHVVYGLVGL KTHCKTCLVV RREGSAIRRY CHIGTGNYNS KTARLYEDVG LLTAAPDIGA DLTDLFNSLT GYSRKVSYRN LLVAPHGIRT GIIERVEREI AAHRERGQGR IRLKMNALVD EQVIDSLYRA SQAGVRVEVV VRGICALRPG VQGYSENIFV RSILGRFLEH SRIIHFRNIN EFWIGSADMM HRNLDRRVEV LAQVKDPKLT AQLDELFESA LDPSTRCWEL GPDGQWTPSP QEGHTVRDHQ VSLMERHRSP
NT Sequence
gtgatgcgcc atgatcgcaa tgtgactgaa atcgacgctg agactcgacc cgacgagaac ctgtggcatt cgggcgactc ggccgtgggg gcgccacccg cggcgacccc ggccgcgatg accgacctgc cggaggaccg ttatctcaac cgggaactga gctggctgga tttcaacgcc cgggtgctgg cgctggccga cgacaactcg ctgccgctgc tggaacgggc caagttcctg gccattttcg cctccaacct cgacgagttc tacatggtgc gggtcgccgg gctgaagcgg cgcgacgaga tgggcttgtc ggtgcgctcg gccgacgggt tgacgccgcg caaacaactc gccctgatcg gcgagcacac ccagcggatc gcgacccggc acgcgcgggt gttcctcgac tcggtgcgcc cggcgctggc cgaggagggc attcacatcg tcacgtgggc cgatctcgat caggccgagc gcgacgaatt gtccacctat ttcaccgaac aggtctttcc cgtcctgacg cccctggccg tcgatcccgc gcacccgttc cccttcgtca gcgggctgag cctgaacctg gcggtgatgg tgcgccagac cgaggacggc ggccagcact tcgcccgcgt caaggtgccc aacaacgtcg atcgcttcgt cgaactcgcg gcgccgagag cgggcgccga gggcgaaaac cgcggcgtcg tccggtttct gccgatggag gaactgatcg cggcgttcct gccgctgctg ttcccgggca tggagatcgt cgaacaccac gcgttccgca tcacccgcaa cgccgacatg gaggtcgagg aggaccgcga cgaagatctg ctgcaggcgc tggaacggga gttggcgcgg cggcgcttcg gcccgccggt gcggctcgag atcgccgacg acatgaccga gggcatgctg gaactgctgc tgcgcgaact cgacgtgcat cccggcgacg tcatcgaagt gcccggcctg ctcgaccttt cgtcgctgcg gcagatctac gatttggatc gccccgcgct caaggatccg gcgttcgtcc cggacaccca ccccgccttc gccgaccgcg aatcccccaa gagcatcttc gcgacgctgc gcgagggcga cgtgctggtg caccacccct acgactcgtt ctccaccagc gtgcaacgct tcatccagca ggccgccgcc gaccccaacg tgctggcgat caagcagacg ctgtaccgca cctccggtga ttcgccgatc gttcgggcgc tgatcgaagc ggccgaggcc ggcaagcagg cggtggcact ggtcgagatc aaggcgcgct tcgacgagca ggccaacatc cgctgggcgc gcgccctcga acaagcgggc gtgcacgtgg tttacgggct cgtcggcctc aagacgcatt gcaagacatg cctggtggtg cgccgcgaag gttcggcgat ccgccggtac tgccacatcg gaaccggcaa ctacaacagc aagaccgccc gcctgtacga ggatgtcggt ctgctgacgg cggcgccgga catcggcgcc gacctcaccg atttgttcaa ttcacttacc ggctattcgc gtaaggtctc ctaccgcaat ctgttggtgg cgccgcacgg aattcgcacc ggcatcatcg aacgcgtcga acgcgagatc gccgcgcacc gcgaacgcgg gcagggccga attcggttga agatgaacgc gttggtggac gagcaggtga tcgactccct gtaccgggcg tcgcaggcgg gggtgcgggt cgaggtcgtg gtgcgcggca tctgcgcgct gcgcccgggc gtgcagggtt actccgaaaa catcttcgtg cgctcgattc tgggacgttt cctggagcac tcgcggatca tccacttccg caacatcaac gaattctgga tcggcagcgc cgatatgatg caccgcaatc tcgatcgccg cgtcgaagtg ctggcgcagg tcaaggatcc caagctcacc gcgcagctgg acgagttgtt cgaatccgcg ctggatccgt ccacccggtg ctgggaattg ggacccgacg ggcagtggac cccgtcgccg caagagggcc ataccgtgcg tgatcaccaa gtatcgttga tggagcggca tcgcagtccc tag
Details for MyavA.19282.a.B2.GE40288
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 78kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDR YLNRELSWLD FNARVLALAD DNSLPLLERA KFLAIFASNL DEFYMVRVAG LKRRDEMGLS VRSADGLTPR KQLALIGEHT QRIATRHARV FLDSVRPALA EEGIHIVTWA DLDQAERDEL STYFTEQVFP VLTPLAVDPA HPFPFVSGLS LNLAVMVRQT EDGGQHFARV KVPNNVDRFV ELAAPRAGAE GENRGVVRFL PMEELIAAFL PLLFPGMEIV EHHAFRITRN ADMEVEEDRD EDLLQALERE LARRRFGPPV RLEIADDMTE GMLELLLREL DVHPGDVIEV PGLLDLSSLR QIYDLDRPAL KDPAFVPDTH PAFADRESPK SIFATLREGD VLVHHPYDSF STSVQRFIQQ AAADPNVLAI KQTLYRTSGD SPIVRALIEA AEAGKQAVAL VEIKARFDEQ ANIRWARALE QAGVHVVYGL VGLKTHCKTC LVVRREGSAI RRYCHIGTGN YNSKTARLYE DVGLLTAAPD IGADLTDLFN SLTGYSRKVS YRNLLVAPHG IRTGIIERVE REIAAHRERG QGRIRLKMNA LVDEQVIDSL YRASQAGVRV EVVVRGICAL RPGVQGYSEN IFVRSILGRF LEHSRIIHFR NINEFWIGSA DMMHRNLDRR VEVLAQVKDP KLTAQLDELF ESALDPSTRC WELGPDGQWT PSPQEGHTVR DHQVSLMERH RSP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaccgtt atctcaaccg ggaactgagc tggctggatt tcaacgcccg ggtgctggcg ctggccgacg acaactcgct gccgctgctg gaacgggcca agttcctggc cattttcgcc tccaacctcg acgagttcta catggtgcgg gtcgccgggc tgaagcggcg cgacgagatg ggcttgtcgg tgcgctcggc cgacgggttg acgccgcgca aacaactcgc cctgatcggc gagcacaccc agcggatcgc gacccggcac gcgcgggtgt tcctcgactc ggtgcgcccg gcgctggccg aggagggcat tcacatcgtc acgtgggccg atctcgatca ggccgagcgc gacgaattgt ccacctattt caccgaacag gtctttcccg tcctgacgcc cctggccgtc gatcccgcgc acccgttccc cttcgtcagc gggctgagcc tgaacctggc ggtgatggtg cgccagaccg aggacggcgg ccagcacttc gcccgcgtca aggtgcccaa caacgtcgat cgcttcgtcg aactcgcggc gccgagagcg ggcgccgagg gcgaaaaccg cggcgtcgtc cggtttctgc cgatggagga actgatcgcg gcgttcctgc cgctgctgtt cccgggcatg gagatcgtcg aacaccacgc gttccgcatc acccgcaacg ccgacatgga ggtcgaggag gaccgcgacg aagatctgct gcaggcgctg gaacgggagt tggcgcggcg gcgcttcggc ccgccggtgc ggctcgagat cgccgacgac atgaccgagg gcatgctgga actgctgctg cgcgaactcg acgtgcatcc cggcgacgtc atcgaagtgc ccggcctgct cgacctttcg tcgctgcggc agatctacga tttggatcgc cccgcgctca aggatccggc gttcgtcccg gacacccacc ccgccttcgc cgaccgcgaa tcccccaaga gcatcttcgc gacgctgcgc gagggcgacg tgctggtgca ccacccctac gactcgttct ccaccagcgt gcaacgcttc atccagcagg ccgccgccga ccccaacgtg ctggcgatca agcagacgct gtaccgcacc tccggtgatt cgccgatcgt tcgggcgctg atcgaagcgg ccgaggccgg caagcaggcg gtggcactgg tcgagatcaa ggcgcgcttc gacgagcagg ccaacatccg ctgggcgcgc gccctcgaac aagcgggcgt gcacgtggtt tacgggctcg tcggcctcaa gacgcattgc aagacatgcc tggtggtgcg ccgcgaaggt tcggcgatcc gccggtactg ccacatcgga accggcaact acaacagcaa gaccgcccgc ctgtacgagg atgtcggtct gctgacggcg gcgccggaca tcggcgccga cctcaccgat ttgttcaatt cacttaccgg ctattcgcgt aaggtctcct accgcaatct gttggtggcg ccgcacggaa ttcgcaccgg catcatcgaa cgcgtcgaac gcgagatcgc cgcgcaccgc gaacgcgggc agggccgaat tcggttgaag atgaacgcgt tggtggacga gcaggtgatc gactccctgt accgggcgtc gcaggcgggg gtgcgggtcg aggtcgtggt gcgcggcatc tgcgcgctgc gcccgggcgt gcagggttac tccgaaaaca tcttcgtgcg ctcgattctg ggacgtttcc tggagcactc gcggatcatc cacttccgca acatcaacga attctggatc ggcagcgccg atatgatgca ccgcaatctc gatcgccgcg tcgaagtgct ggcgcaggtc aaggatccca agctcaccgc gcagctggac gagttgttcg aatccgcgct ggatccgtcc acccggtgct gggaattggg acccgacggg cagtggaccc cgtcgccgca agagggccat accgtgcgtg atcaccaagt atcgttgatg gagcggcatc gcagtccctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MyavA.19282.a.B2.PS38267
PURIFICATION DATe: 4/28/2017
CONCENTRATION: 9mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHDR YLNRELSWLD FNARVLALAD DNSLPLLERA KFLAIFASNL DEFYMVRVAG LKRRDEMGLS VRSADGLTPR KQLALIGEHT QRIATRHARV FLDSVRPALA EEGIHIVTWA DLDQAERDEL STYFTEQVFP VLTPLAVDPA HPFPFVSGLS LNLAVMVRQT EDGGQHFARV KVPNNVDRFV ELAAPRAGAE GENRGVVRFL PMEELIAAFL PLLFPGMEIV EHHAFRITRN ADMEVEEDRD EDLLQALERE LARRRFGPPV RLEIADDMTE GMLELLLREL DVHPGDVIEV PGLLDLSSLR QIYDLDRPAL KDPAFVPDTH PAFADRESPK SIFATLREGD VLVHHPYDSF STSVQRFIQQ AAADPNVLAI KQTLYRTSGD SPIVRALIEA AEAGKQAVAL VEIKARFDEQ ANIRWARALE QAGVHVVYGL VGLKTHCKTC LVVRREGSAI RRYCHIGTGN YNSKTARLYE DVGLLTAAPD IGADLTDLFN SLTGYSRKVS YRNLLVAPHG IRTGIIERVE REIAAHRERG QGRIRLKMNA LVDEQVIDSL YRASQAGVRV EVVVRGICAL RPGVQGYSEN IFVRSILGRF LEHSRIIHFR NINEFWIGSA DMMHRNLDRR VEVLAQVKDP KLTAQLDELF ESALDPSTRC WELGPDGQWT PSPQEGHTVR DHQVSLMERH RSP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGACCGTT ATCTCAACCG GGAACTGAGC TGGCTGGATT TCAACGCCCG GGTGCTGGCG CTGGCCGACG ACAACTCGCT GCCGCTGCTG GAACGGGCCA AGTTCCTGGC CATTTTCGCC TCCAACCTCG ACGAGTTCTA CATGGTGCGG GTCGCCGGGC TGAAGCGGCG CGACGAGATG GGCTTGTCGG TGCGCTCGGC CGACGGGTTG ACGCCGCGCA AACAACTCGC CCTGATCGGC GAGCACACCC AGCGGATCGC GACCCGGCAC GCGCGGGTGT TCCTCGACTC GGTGCGCCCG GCGCTGGCCG AGGAGGGCAT TCACATCGTC ACGTGGGCCG ATCTCGATCA GGCCGAGCGC GACGAATTGT CCACCTATTT CACCGAACAG GTCTTTCCCG TCCTGACGCC CCTGGCCGTC GATCCCGCGC ACCCGTTCCC CTTCGTCAGC GGGCTGAGCC TGAACCTGGC GGTGATGGTG CGCCAGACCG AGGACGGCGG CCAGCACTTC GCCCGCGTCA AGGTGCCCAA CAACGTCGAT CGCTTCGTCG AACTCGCGGC GCCGAGAGCG GGCGCCGAGG GCGAAAACCG CGGCGTCGTC CGGTTTCTGC CGATGGAGGA ACTGATCGCG GCGTTCCTGC CGCTGCTGTT CCCGGGCATG GAGATCGTCG AACACCACGC GTTCCGCATC ACCCGCAACG CCGACATGGA GGTCGAGGAG GACCGCGACG AAGATCTGCT GCAGGCGCTG GAACGGGAGT TGGCGCGGCG GCGCTTCGGC CCGCCGGTGC GGCTCGAGAT CGCCGACGAC ATGACCGAGG GCATGCTGGA ACTGCTGCTG CGCGAACTCG ACGTGCATCC CGGCGACGTC ATCGAAGTGC CCGGCCTGCT CGACCTTTCG TCGCTGCGGC AGATCTACGA TTTGGATCGC CCCGCGCTCA AGGATCCGGC GTTCGTCCCG GACACCCACC CCGCCTTCGC CGACCGCGAA TCCCCCAAGA GCATCTTCGC GACGCTGCGC GAGGGCGACG TGCTGGTGCA CCACCCCTAC GACTCGTTCT CCACCAGCGT GCAACGCTTC ATCCAGCAGG CCGCCGCCGA CCCCAACGTG CTGGCGATCA AGCAGACGCT GTACCGCACC TCCGGTGATT CGCCGATCGT TCGGGCGCTG ATCGAAGCGG CCGAGGCCGG CAAGCAGGCG GTGGCACTGG TCGAGATCAA GGCGCGCTTC GACGAGCAGG CCAACATCCG CTGGGCGCGC GCCCTCGAAC AAGCGGGCGT GCACGTGGTT TACGGGCTCG TCGGCCTCAA GACGCATTGC AAGACATGCC TGGTGGTGCG CCGCGAAGGT TCGGCGATCC GCCGGTACTG CCACATCGGA ACCGGCAACT ACAACAGCAA GACCGCCCGC CTGTACGAGG ATGTCGGTCT GCTGACGGCG GCGCCGGACA TCGGCGCCGA CCTCACCGAT TTGTTCAATT CACTTACCGG CTATTCGCGT AAGGTCTCCT ACCGCAATCT GTTGGTGGCG CCGCACGGAA TTCGCACCGG CATCATCGAA CGCGTCGAAC GCGAGATCGC CGCGCACCGC GAACGCGGGC AGGGCCGAAT TCGGTTGAAG ATGAACGCGT TGGTGGACGA GCAGGTGATC GACTCCCTGT ACCGGGCGTC GCAGGCGGGG GTGCGGGTCG AGGTCGTGGT GCGCGGCATC TGCGCGCTGC GCCCGGGCGT GCAGGGTTAC TCCGAAAACA TCTTCGTGCG CTCGATTCTG GGACGTTTCC TGGAGCACTC GCGGATCATC CACTTCCGCA ACATCAACGA ATTCTGGATC GGCAGCGCCG ATATGATGCA CCGCAATCTC GATCGCCGCG TCGAAGTGCT GGCGCAGGTC AAGGATCCCA AGCTCACCGC GCAGCTGGAC GAGTTGTTCG AATCCGCGCT GGATCCGTCC ACCCGGTGCT GGGAATTGGG ACCCGACGGG CAGTGGACCC CGTCGCCGCA AGAGGGCCAT ACCGTGCGTG ATCACCAAGT ATCGTTGATG GAGCGGCATC GCAGTCCCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT