MyavA.19278.a

Cytochrome c family protein

CENTER ID: MyavA.19278.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.19278.a.B2.GE40287 cytochrome c-like domain 50 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A0A0H2ZR30

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKLRSTRTD RSRRRLRRRL SGGLLLLIAL TIAGGLAAIL TPRPQVAVAD ESNSALLRTG KQLFDTSCVS CHGANLQGVP DRGPSLIGVG EEAVYFQVST GRMPAMTGEA QAPRKEPVFD EAQTDALGAY VQANGGGPTT VRNPDGSLAM RSLRGEDLGR GGDLFRLNCS SCHNFTGKGG ALSSGKYAPD LEPANEQQIL AAMRTGPQNM PKFSDRQLSF EAKKDIIGYI KAVTEERQPG GYGLGGFGPA PEGMAAWIIG MVAAIGLALW IGARA
NT Sequence
ttgaagaagc tgcggtccac ccgaaccgac cgttcgcgac ggcggctgcg ccgccgattg tccggcggcc tgctgttgct gatcgcgctg accatcgccg gcggactggc cgccatcctg acgccgcggc cgcaggtcgc cgtcgccgac gagtcgaact cggcgctgct gcgcaccggc aagcagctgt tcgacacgtc gtgcgtgtcg tgccacggcg ccaacctgca gggcgtgccc gaccgcgggc cgagcctgat cggtgtcggt gaggaggccg tctacttcca ggtgtccacc ggccggatgc ccgcgatgac gggcgaggcg caggcgcccc gcaaggagcc ggtgttcgac gaggcgcaga ccgacgcgct gggcgcctac gtgcaggcca acggcggcgg ccccaccacg gtgcgcaacc cggatggcag cctggcgatg cgctcgctgc gcggcgagga cctgggccgc ggcggcgatc tgttccggct gaactgctcg tcgtgccaca acttcaccgg caagggcggg gcgctgtcgt ccggcaagta cgcgccggac ctcgagcccg ccaacgagca gcagatcctg gcggcgatgc ggaccggtcc gcagaacatg ccgaagttct ccgaccgcca gctgtcgttc gaggccaaga aggacatcat cggctacatc aaggcggtga ccgaggagcg ccagccgggc ggctacggcc tgggcgggtt cggccccgcc cccgagggca tggcggcctg gatcatcggc atggtggccg ccatcgggct ggcgctgtgg atcggggcac gcgcatga
Details for MyavA.19278.a.B2.GE40287
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 21kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD ESNSALLRTG KQLFDTSCVS CHGANLQGVP DRGPSLIGVG EEAVYFQVST GRMPAMTGEA QAPRKEPVFD EAQTDALGAY VQANGGGPTT VRNPDGSLAM RSLRGEDLGR GGDLFRLNCS SCHNFTGKGG ALSSGKYAPD LEPANEQQIL AAMRTGPQNM PKFSDRQLSF EAKKDIIGYI KAVTEERQPG
Validated NT Sequence
atcttactca cttagcccgg ctggcgctcc tcggtcaccg ccttgatgta gccgatgatg tccttcttgg cctcgaacga cagctggcgg tcggagaact tcggcatgtt ctgcggaccg gtccgcatcg ccgccaggat ctgctgctcg ttggcgggct cgaggtccgg cgcgtacttg ccggacgaca gcgccccgcc cttgccggtg aagttgtggc acgacgagca gttcagccgg aacagatcgc cgccgcggcc caggtcctcg ccgcgcagcg agcgcatcgc caggctgcca tccgggttgc gcaccgtggt ggggccgccg ccgttggcct gcacgtaggc gcccagcgcg tcggtctgcg cctcgtcgaa caccggctcc ttgcggggcg cctgcgcctc gcccgtcatc gcgggcatcc ggccggtgga cacctggaag tagacggcct cctcaccgac accgatcagg ctcggcccgc ggtcgggcac gccctgcagg ttggcgccgt ggcacgacac gcacgacgtg tcgaacagct gcttgccggt gcgcagcagc gccgagttcg actcgtccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHDE SNSALLRTGK QLFDTSCVSC HGANLQGVPD RGPSLIGVGE EAVYFQVSTG RMPAMTGEAQ APRKEPVFDE AQTDALGAYV QANGGGPTTV RNPDGSLAMR SLRGEDLGRG GDLFRLNCSS CHNFTGKGGA LSSGKYAPDL EPANEQQILA AMRTGPQNMP KFSDRQLSFE AKKDIIGYIK AVTEERQPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgagt cgaactcggc gctgctgcgc accggcaagc agctgttcga cacgtcgtgc gtgtcgtgcc acggcgccaa cctgcagggc gtgcccgacc gcgggccgag cctgatcggt gtcggtgagg aggccgtcta cttccaggtg tccaccggcc ggatgcccgc gatgacgggc gaggcgcagg cgccccgcaa ggagccggtg ttcgacgagg cgcagaccga cgcgctgggc gcctacgtgc aggccaacgg cggcggcccc accacggtgc gcaacccgga tggcagcctg gcgatgcgct cgctgcgcgg cgaggacctg ggccgcggcg gcgatctgtt ccggctgaac tgctcgtcgt gccacaactt caccggcaag ggcggggcgc tgtcgtccgg caagtacgcg ccggacctcg agcccgccaa cgagcagcag atcctggcgg cgatgcggac cggtccgcag aacatgccga agttctccga ccgccagctg tcgttcgagg ccaagaagga catcatcggc tacatcaagg cggtgaccga ggagcgccag ccgggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t