MyavA.18684.a

Pup--protein ligase (PafA)

CENTER ID: MyavA.18684.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.18684.a.A1.GE45371 full length 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
UniProt: A0QFB7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRRIMGIET EFGVTCTFHG HRRLSPDEVA RYLFRRVVSW GRSSNVFLRN GARLYLDVGS HPEYATAECD NLVQLVTHDR AGEWVLEDLL VDAEQRLADE GIGGDIYLFK NNTDSAGNSY GCHENYLIVR AGEFSRISDV LLPFLVTRQL ICGAGKVLQT PKAATFCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA EKYRRLHVIV GDSNMCETTT MLKVGTAALV LEMIEAGVPF RDFSLDNPIR AIREVSHDIT GRRPVRLAGG RQASALDIQR EYYSRAVEHL QTREPNAQIE QIVDLWGRQL DAVESQDFAK VDTEIDWVIK RKLFQRYQDR YNMELSDPKI AQLDLAYHDI KRGRGVFDLL QRKGLAARVT TDEDIAEAVD TPPQTTRARL RGEFISAAQA AGRDFTVDWV HLKLNDQAQR TVLCKDPFRA VDERVKRLIA SM
NT Sequence
gtgcagcggc gaatcatggg catcgagacc gagttcgggg tcacgtgcac attccatggg catcgccggc tcagcccgga cgaggtggcc cgctacctgt tccgccgcgt cgtgtcctgg ggccgcagct ccaacgtctt cctgcggaac ggggcccggc tctacctcga cgtgggcagc caccccgagt acgccaccgc cgaatgcgac aacctggtgc agctggtcac ccacgaccgc gccggggaat gggtgctcga ggacctgctc gtcgatgccg agcagcggct ggccgacgag ggcatcggcg gcgacatcta cctgttcaag aacaacaccg actcggcggg caactcctac ggctgccacg agaactacct gatcgtgcgg gccggcgagt tctcccggat ctccgatgta ctgctgccgt tcctggtcac ccgccagctg atctgcgggg ccggcaaggt gctgcagacg ccgaaggctg cgacgttctg cctttcccaa cgcgccgagc acatttggga gggcgtctcc agcgccacca cccgcagccg cccgatcatc aacacccgcg acgagccgca cgccgacgcc gagaaatacc ggcggctgca cgtcatcgtc ggcgactcca acatgtgcga aaccaccacc atgctcaagg tgggtaccgc ggcgctggtg ctggagatga tcgaggccgg cgtcccgttc cgggacttct cgctggacaa cccgatccgt gcgatccgcg aggtcagcca cgacatcacc ggccggcggc cggtccggct ggccgggggg cgtcaggcca gcgcgctgga catccagcgc gagtactaca gccgcgccgt cgaacacctg cagacccggg agcccaacgc ccagatcgaa cagatcgtcg acctgtgggg ccgccaactc gacgcggtcg aaagccagga cttcgccaag gtggacaccg agatcgactg ggtgatcaag cgcaagctgt tccagcgcta ccaggaccgc tacaacatgg agctgtccga cccgaagatc gcccagctgg acctggccta ccacgacatc aagcgcggcc gcggcgtctt cgatctgctg cagcgcaagg ggctcgccgc ccgggtcacc accgacgagg acatcgccga ggcggtcgac acgccgccgc agaccacccg ggcgcggctg cgcggcgagt tcatcagcgc cgcccaggcc gccggccgcg acttcaccgt cgactgggtg cacctcaagc tcaacgacca ggcgcagcgg accgtgctgt gcaaggaccc gttccgggcc gtcgacgagc gggtcaagcg gctgatcgcg agcatgtag
Details for MyavA.18684.a.A1.GE45371
HARVESTED ON: data unavailable
SEQUENCED ON: data unavailable
EXPECTED MW: 54kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRRIMGIE TEFGVTCTFH GHRRLSPDEV ARYLFRRVVS WGRSSNVFLR NGARLYLDVG SHPEYATAEC DNLVQLVTHD RAGEWVLEDL LVDAEQRLAD EGIGGDIYLF KNNTDSAGNS YGCHENYLIV RAGEFSRISD VLLPFLVTRQ LICGAGKVLQ TPKAATFCLS QRAEHIWEGV SSATTRSRPI INTRDEPHAD AEKYRRLHVI VGDSNMCETT TMLKVGTAAL VLEMIEAGVP FRDFSLDNPI RAIREVSHDI TGRRPVRLAG GRQASALDIQ REYYSRAVEH LQTREPNAQI EQIVDLWGRQ LDAVESQDFA KVDTEIDWVI KRKLFQRYQD RYNMELSDPK IAQLDLAYHD IKRGRGVFDL LQRKGLAARV TTDEDIAEAV DTPPQTTRAR LRGEFISAAQ AAGRDFTVDW VHLKLNDQAQ RTVLCKDPFR AVDERVKRLI ASM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcagcggcga atcatgggca tcgagaccga gttcggggtc acgtgcacat tccatgggca tcgccggctc agcccggacg aggtggcccg ctacctgttc cgccgcgtcg tgtcctgggg ccgcagctcc aacgtcttcc tgcggaacgg ggcccggctc tacctcgacg tgggcagcca ccccgagtac gccaccgccg aatgcgacaa cctggtgcag ctggtcaccc acgaccgcgc cggggaatgg gtgctcgagg acctgctcgt cgatgccgag cagcggctgg ccgacgaggg catcggcggc gacatctacc tgttcaagaa caacaccgac tcggcgggca actcctacgg ctgccacgag aactacctga tcgtgcgggc cggcgagttc tcccggatct ccgatgtact gctgccgttc ctggtcaccc gccagctgat ctgcggggcc ggcaaggtgc tgcagacgcc gaaggctgcg acgttctgcc tttcccaacg cgccgagcac atttgggagg gcgtctccag cgccaccacc cgcagccgcc cgatcatcaa cacccgcgac gagccgcacg ccgacgccga gaaataccgg cggctgcacg tcatcgtcgg cgactccaac atgtgcgaaa ccaccaccat gctcaaggtg ggtaccgcgg cgctggtgct ggagatgatc gaggccggcg tcccgttccg ggacttctcg ctggacaacc cgatccgtgc gatccgcgag gtcagccacg acatcaccgg ccggcggccg gtccggctgg ccggggggcg tcaggccagc gcgctggaca tccagcgcga gtactacagc cgcgccgtcg aacacctgca gacccgggag cccaacgccc agatcgaaca gatcgtcgac ctgtggggcc gccaactcga cgcggtcgaa agccaggact tcgccaaggt ggacaccgag atcgactggg tgatcaagcg caagctgttc cagcgctacc aggaccgcta caacatggag ctgtccgacc cgaagatcgc ccagctggac ctggcctacc acgacatcaa gcgcggccgc ggcgtcttcg atctgctgca gcgcaagggg ctcgccgccc gggtcaccac cgacgaggac atcgccgagg cggtcgacac gccgccgcag accacccggg cgcggctgcg cggcgagttc atcagcgccg cccaggccgc cggccgcgac ttcaccgtcg actgggtgca cctcaagctc aacgaccagg cgcagcggac cgtgctgtgc aaggacccgt tccgggccgt cgacgagcgg gtcaagcggc tgatcgcgag catgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa