SSGCID: Target Page

MyavA.18665.a

ABC1 family protein

CENTER ID:	MyavA.18665.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.18665.a.B2.GE40286	MythA crystallizable domain		42	412

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.18665.a.MB1.PS02390	full length		1	444

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.4434
RefSeq:	YP_883648.1
UniProt:	A0A0H2ZZK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKLDRVPLP VEAARVAATG WQVTRSAARV LTKLPAKGPW QQKVIKQLPQ TFADLGPTYV KFGQIIASSP GAFGESLSRE FRGLLDRVPP ADPDEVHKLF LEELGADPSE LFASWDETPF ASASIAQVHY ATLHTGEEVV VKIQRPGIRR RVAADLQILK RFAQAVELAK LGRRLSAQDV VADFSDNLAE ELNFRLEAQS MEAWVSHLHA SPLGKNIRVP QVYWDFTTDR VLTMERVSGI RIDDVAAVRK AGFDGVELVK ALLFSLFEGG LRHGLFHGDL HAGNLYVDEA GRIVFFDFGI MGRIDPRTRW LLRELVYALL VKKDHAAAGK IVVLMGAVGT VKPEAQAAKD LEKFATPLTM QTLGDMSYAD IGRQLSALAD AYDVKLPREL VLIGKQFLYV ERYMKLLAPK WQMMSDPQLT GYFANFMVEV SREHSSEFES DVEV

NT Sequence

gtggccaagc tcgaccgggt gccgttgccg gtcgaggcgg cccgggtagc ggcgaccggt tggcaggtca cccgctccgc cgctcgcgtc ctcaccaagc tgccggccaa gggcccgtgg cagcagaagg tgatcaagca gctcccccag acgttcgccg acctggggcc cacctacgtc aagttcgggc agatcatcgc gtccagcccc ggcgcgttcg gcgaatcgct gtcccgggag ttccgcggcc tgctggaccg ggtgccgccc gccgaccccg acgaggtgca caagctgttc ctcgaggagc tcggggccga cccgtccgag ctgttcgcca gctgggacga gaccccgttc gcctcggcgt ccatcgccca ggtgcactac gccaccctgc acaccggcga ggaggtcgtc gtcaagatcc agcggccggg catccgccgc cgggtcgccg ccgacctgca gatcctcaag cgcttcgccc aggccgtcga gctggccaag ctgggccgcc ggctgtccgc ccaggacgtg gtcgccgact tctccgacaa cctggccgag gagctgaatt tccggctgga ggcccagtcg atggaggcct gggtgtcgca cctgcacgcc tcgccgctgg gcaaaaacat ccgggtgccg caggtgtact gggacttcac caccgaccgg gtgctgacca tggagcgggt gtccgggatc cgcatcgacg acgtcgccgc ggtccgcaag gccgggttcg acggggttga gctggtcaag gcgctgctgt tcagcctgtt cgagggcggg ctgcggcacg gcctgttcca cggcgacctg cacgccggca acctctacgt cgacgaggcg ggccgcatcg tgttcttcga cttcggcatc atgggccgca tcgacccgcg cacccgctgg ctgctgcgcg agctggtgta tgcgctgctg gtcaagaagg accacgccgc ggccggcaag atcgtggtgc tgatgggcgc cgtgggcacc gtgaaacccg aagcccaggc cgccaaggac ctggagaaat tcgccacccc gctgaccatg cagaccctgg gcgacatgtc ctatgccgac atcggccgcc agctgtcggc gctggccgac gcctacgacg tcaagctgcc ccgcgagctg gtgctgatcg gcaagcagtt cctctacgtc gagcgctaca tgaagctgct ggcacccaag tggcagatga tgtcggatcc gcaattgacc gggtatttcg ccaacttcat ggtcgaggtc agccgcgagc actcctccga gttcgaatcc gacgtcgagg tctag

Details for MyavA.18665.a.B2.GE40286

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	42kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ KVIKQLPQTF ADLGPTYVKF GQIIASSPGA FGESLSREFR GLLDRVPPAD PDEVHKLFLE ELGADPSELF ASWDETPFAS ASIAQVHYAT LHTGEEVVVK IQRPGIRRRV AADLQILKRF AQAVELAKLG RRLSAQDVVA DFSDNLAEEL NFRLEAQSME AWVSHLHASP LGKNIRVPQV YWDFTTDRVL TMERVSGIRI DDVAAVRKAG FDGVELVKAL LFSLFEGGLR HGLFHGDLHA GNLYVDEAGR IVFFDFGIMG RIDPRTRWLL RELVYALLVK KDHAAAGKIV VLMGAVGTVK PEAQAAKDLE KFATPLTMQT LGDMSYADIG RQLSALADAY DVKLPRELVL IGKQFLYVER

Validated NT Sequence

tagcgctcga cgtagaggaa ctgcttgccg atcagcacca gctcgcgggg cagcttgacg tcgtaggcgt cggccagcgc cgacagctgg cggccgatgt cggcatagga catgtcgccc agggtctgca tggtcagcgg ggtggcgaat ttctccaggt ccttggcggc ctgggcttcg ggtttcacgg tgcccacggc gcccatcagc accacgatct tgccggccgc ggcgtggtcc ttcttgacca gcagcgcata caccagctcg cgcagcagcc agcgggtgcg cgggtcgatg cggcccatga tgccgaagtc gaagaacacg atgcggcccg cctcgtcgac gtagaggttg ccggcgtgca ggtcgccgtg gaacaggccg tgccgcagcc cgccctcgaa caggctgaac agcagcgcct tgaccagctc aaccccgtcg aacccggcct tgcggaccgc ggcgacgtcg tcgatgcgga tcccggacac ccgctccatg gtcagcaccc ggtcggtggt gaagtcccag tacacctgcg gcacccggat gtttttgccc agcggcgagg cgtgcaggtg cgacacccag gcctccatcg actgggcctc cagccggaaa ttcagctcct cggccaggtt gtcggagaag tcggcgacca cgtcctgggc ggacagccgg cggcccagct tggccagctc gacggcctgg gcgaagcgct tgaggatctg caggtcggcg gcgacccggc ggcggatgcc cggccgctgg atcttgacga cgacctcctc gccggtgtgc agggtggcgt agtgcacctg ggcgatggac gccgaggcga acggggtctc gtcccagctg gcgaacagct cggacgggtc ggccccgagc tcctcgagga acagcttgtg cacctcgtcg gggtcggcgg gcggcacccg gtccagcagg ccgcggaact cccgggacag cgattcgccg aacgcgccgg ggctggacgc gatgatctgc ccgaacttga cgtaggtggg ccccaggtcg gcgaacgtct gggggagctg cttgatcacc ttctgcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHQK VIKQLPQTFA DLGPTYVKFG QIIASSPGAF GESLSREFRG LLDRVPPADP DEVHKLFLEE LGADPSELFA SWDETPFASA SIAQVHYATL HTGEEVVVKI QRPGIRRRVA ADLQILKRFA QAVELAKLGR RLSAQDVVAD FSDNLAEELN FRLEAQSMEA WVSHLHASPL GKNIRVPQVY WDFTTDRVLT MERVSGIRID DVAAVRKAGF DGVELVKALL FSLFEGGLRH GLFHGDLHAG NLYVDEAGRI VFFDFGIMGR IDPRTRWLLR ELVYALLVKK DHAAAGKIVV LMGAVGTVKP EAQAAKDLEK FATPLTMQTL GDMSYADIGR QLSALADAYD VKLPRELVLI GKQFLYVERY MKLLAPKWQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagaagg tgatcaagca gctcccccag acgttcgccg acctggggcc cacctacgtc aagttcgggc agatcatcgc gtccagcccc ggcgcgttcg gcgaatcgct gtcccgggag ttccgcggcc tgctggaccg ggtgccgccc gccgaccccg acgaggtgca caagctgttc ctcgaggagc tcggggccga cccgtccgag ctgttcgcca gctgggacga gaccccgttc gcctcggcgt ccatcgccca ggtgcactac gccaccctgc acaccggcga ggaggtcgtc gtcaagatcc agcggccggg catccgccgc cgggtcgccg ccgacctgca gatcctcaag cgcttcgccc aggccgtcga gctggccaag ctgggccgcc ggctgtccgc ccaggacgtg gtcgccgact tctccgacaa cctggccgag gagctgaatt tccggctgga ggcccagtcg atggaggcct gggtgtcgca cctgcacgcc tcgccgctgg gcaaaaacat ccgggtgccg caggtgtact gggacttcac caccgaccgg gtgctgacca tggagcgggt gtccgggatc cgcatcgacg acgtcgccgc ggtccgcaag gccgggttcg acggggttga gctggtcaag gcgctgctgt tcagcctgtt cgagggcggg ctgcggcacg gcctgttcca cggcgacctg cacgccggca acctctacgt cgacgaggcg ggccgcatcg tgttcttcga cttcggcatc atgggccgca tcgacccgcg cacccgctgg ctgctgcgcg agctggtgta tgcgctgctg gtcaagaagg accacgccgc ggccggcaag atcgtggtgc tgatgggcgc cgtgggcacc gtgaaacccg aagcccaggc cgccaaggac ctggagaaat tcgccacccc gctgaccatg cagaccctgg gcgacatgtc ctatgccgac atcggccgcc agctgtcggc gctggccgac gcctacgacg tcaagctgcc ccgcgagctg gtgctgatcg gcaagcagtt cctctacgtc gagcgctaca tgaagctgct ggcacccaag tggcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for MyavA.18665.a.MB1.PS02390

PURIFICATION DATe:	4/14/2015
CONCENTRATION:	64.4mg/ml
OBSERVED MW:	95kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MAKLDRVPLP VEAARVAATG WQVTRSAARV LTKLPAKGPW QQKVIKQLPQ TFADLGPTYV KFGQIIASSP GAFGESLSRE FRGLLDRVPP ADPDEVHKLF LEELGADPSE LFASWDETPF ASASIAQVHY ATLHTGEEVV VKIQRPGIRR RVAADLQILK RFAQAVELAK LGRRLSAQDV VADFSDNLAE ELNFRLEAQS MEAWVSHLHA SPLGKNIRVP QVYWDFTTDR VLTMERVSGI RIDDVAAVRK AGFDGVELVK ALLFSLFEGG LRHGLFHGDL HAGNLYVDEA GRIVFFDFGI MGRIDPRTRW LLRELVYALL VKKDHAAAGK IVVLMGAVGT VKPEAQAAKD LEKFATPLTM QTLGDMSYAD IGRQLSALAD AYDVKLPREL VLIGKQFLYV ERYMKLLAPK WQMMSDPQLT GYFANFMVEV SREHSSEFES DVEV

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAATAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGGTGGC CAAGCTCGAC CGGGTGCCGT TGCCGGTCGA GGCGGCCCGG GTAGCGGCGA CCGGTTGGCA GGTCACCCGC TCCGCCGCTC GCGTCCTCAC CAAGCTGCCG GCCAAGGGCC CGTGGCAGCA GAAGGTGATC AAGCAGCTCC CCCAGACGTT CGCCGACCTG GGGCCCACCT ACGTCAAGTT CGGGCAGATC ATCGCGTCCA GCCCCGGCGC GTTCGGCGAA TCGCTGTCCC GGGAGTTCCG CGGCCTGCTG GACCGGGTGC CGCCCGCCGA CCCCGACGAG GTGCACAAGC TGTTCCTCGA GGAGCTCGGG GCCGACCCGT CCGAGCTGTT CGCCAGCTGG GACGAGACCC CGTTCGCCTC GGCGTCCATC GCCCAGGTGC ACTACGCCAC CCTGCACACC GGCGAGGAGG TCGTCGTCAA GATCCAGCGG CCGGGCATCC GCCGCCGGGT CGCCGCCGAC CTGCAGATCC TCAAGCGCTT CGCCCAGGCC GTCGAGCTGG CCAAGCTGGG CCGCCGGCTG TCCGCCCAGG ACGTGGTCGC CGACTTCTCC GACAACCTGG CCGAGGAGCT GAATTTCCGG CTGGAGGCCC AGTCGATGGA GGCCTGGGTG TCGCACCTGC ACGCCTCGCC GCTGGGCAAA AACATCCGGG TGCCGCAGGT GTACTGGGAC TTCACCACCG ACCGGGTGCT GACCATGGAG CGGGTGTCCG GGATCCGCAT CGACGACGTC GCCGCGGTCC GCAAGGCCGG GTTCGACGGG GTTGAGCTGG TCAAGGCGCT GCTGTTCAGC CTGTTCGAGG GCGGGCTGCG GCACGGCCTG TTCCACGGCG ACCTGCACGC CGGCAACCTC TACGTCGACG AGGCGGGCCG CATCGTGTTC TTCGACTTCG GCATCATGGG CCGCATCGAC CCGCGCACCC GCTGGCTGCT GCGCGAGCTG GTGTATGCGC TGCTGGTCAA GAAGGACCAC GCCGCGGCCG GCAAGATCGT GGTGCTGATG GGCGCCGTGG GCACCGTGAA ACCCGAAGCC CAGGCCGCCA AGGACCTGGA GAAATTCGCC ACCCCGCTGA CCATGCAGAC CCTGGGCGAC ATGTCCTATG CCGACATCGG CCGCCAGCTG TCGGCGCTGG CCGACGCCTA CGACGTCAAG CTGCCCCGCG AGCTGGTGCT GATCGGCAAG CAGTTCCTCT ACGTCGAGCG CTACATGAAG CTGCTGGCAC CCAAGTGGCA GATGATGTCG GATCCGCAAT TGACCGGGTA TTTCGCCAAC TTCATGGTCG AGGTCAGCCG CGAGCACTCC TCCGAGTTCG AATCCGACGT CGAGGTCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA