MyavA.18628.a

Mce-family protein mce2f (Rv0174 ortholog)

CENTER ID: MyavA.18628.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.18628.a.B2.GE37419 mature protein 21 508
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.4463
RefSeq: YP_883677.1
UniProt: A0A0H2ZUG4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKTQLVLLT AVAVAAVVVL GWYYLRIPSL AGIGRYTLYA ELPQSGGLYR TANVTYRGIT IGKVTGVEPT ERGARATMSI EDGYRIPADA AAHVHSVSAV GEQYVDLVST AGREPYLVDG QTIHKSTVPS QIGPALDAAN RGLAVLPRDK IASLLYETSQ AVGGLGPSLR RLVDATQAIA HDFRGSIDHV DDIVERSAPI IDSQADSADT LGRWAANLNT LAAQTARQDP ALRSILANAA PTAEQVRATF GGVRESLPQT LANLEVVIDM LKRYHNGVEQ ALVFLPQSGA IAQSVTAQSP GQAALGVGAI SLNQPPPCLT GFLPASQWRA PADTSTAPLP AGTYCKIPMD ATNVVRGARN YPCVDVPGKR AATPRECRST EPYVPQGTNP WYGDPDQILT CPAPGARCDQ PVKPGLVIPA PSVDNGLNPL PADRLPGTPP PISDPLQRPG SGTVVCNGQQ PNPCTYTPSA LYDVRSGTVV GPDGVVYSVA NSATIGDEGW KTMLGQAR
NT Sequence
atgatcaaaa cccagctggt gctgttgacg gcggtggcgg tggccgcggt ggtcgtcctc ggctggtact acctgcggat cccgagcctg gccggtatcg gccgctacac cctgtacgcc gaattgccgc aatccggcgg cctgtaccgg accgccaacg tcacctaccg gggaatcacc atcggcaagg tcaccggcgt cgagccgacc gagcgtggcg cgcgggcgac gatgagcatc gaggacggct accggatccc ggccgatgcc gccgcccacg tgcattcggt ctcggcggtg ggcgagcagt acgtcgacct ggtctcgacc gccgggcgcg aaccgtatct ggtcgacggg cagaccatcc acaaaagcac ggtgcccagc cagatcggtc ccgcgctgga cgccgccaac cgcggactgg cggtgctgcc gcgggacaag atcgcctcgc tgctctacga aacctcgcag gcggtcggtg gcctggggcc gtcgctgcgg cgcctggtgg atgccaccca ggcgatcgcc cacgatttcc ggggcagcat cgaccacgtc gacgacatcg tcgagcgctc ggcaccgatc atcgacagcc aggccgattc cgctgacacg cttgggcgtt gggccgccaa cctgaacacg ttggccgccc agaccgcgcg gcaggacccg gcgctgcgca gcatcctggc caacgcggcg ccgacggccg agcaggtgcg ggccacgttc ggcggtgtgc gggagtcgct gccgcagacg ctggccaatc tcgaggtcgt gatcgacatg ctcaagcgct atcacaacgg cgtcgagcag gcgttggtgt tcctgccgca gtccggcgcg atcgcccagt cggtgaccgc gcaatcgccg ggccaggccg cactgggcgt cggcgccatc tcgctcaacc agccgccgcc ttgcctgacc ggtttcctgc cggcatcgca gtggcgcgcc ccggcggaca ccagcacggc gccgctgccg gccggcacct actgcaagat cccgatggac gccaccaatg tcgttcgggg agcccgcaat tacccgtgcg tggacgtgcc gggcaagcgg gcggccacac cgcgggagtg ccgcagcacc gagccctatg tgccgcaggg caccaacccc tggtacggcg accccgacca gatactgacc tgtccggcgc cgggcgcgcg gtgcgaccaa ccggtgaagc cgggcctggt gatccccgca ccctcggtcg acaacggcct caatccgctg cccgccgacc ggctgccggg caccccaccg ccgatcagcg acccgctgca gcggccggga tcgggcaccg tggtgtgcaa cgggcagcag cccaacccgt gcacgtacac ccccagcgcg ctctacgacg tgcgcagcgg cacggtggtg ggccccgacg gggtggtgta ttcggtggcc aattcggcga ccatcggcga cgaggggtgg aagacgatgc tgggacaggc ccgctga
Details for MyavA.18628.a.B2.GE37419
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 52kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG WYYLRIPSLA GIGRYTLYAE LPQSGGLYRT ANVTYRGITI GKVTGVEPTE RGARATMSIE DGYRIPADAA AHVHSVSAVG EQYVDLVSTA GREPYLVDGQ TIHKSTVPSQ IGPALDAANR GLAVLPRDKI ASLLYETSQA VGGLGPSLRR LVDATQAIAH DFRGSIDHVD DIVERSAPII DSQADSADTL GRWAANLNTL AAQTARQDPA LRSILANAAP TAEQVRATFG GVRESLPQTL ANLEVVIDML KRYHNGVEQA LVFLPQSGAI AQSVTAQSPG XAALGVXAIS LNQPPPCLTG XLXXIXVARP AXXSTXXCXX XXLXIPXXAX XXXGXXXPXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggct ggtactacct gcggatcccg agcctggccg gtatcggccg ctacaccctg tacgccgaat tgccgcaatc cggcggcctg taccggaccg ccaacgtcac ctaccgggga atcaccatcg gcaaggtcac cggcgtcgag ccgaccgagc gtggcgcgcg ggcgacgatg agcatcgagg acggctaccg gatcccggcc gatgccgccg cccacgtgca ttcggtctcg gcggtgggcg agcagtacgt cgacctggtc tcgaccgccg ggcgcgaacc gtatctggtc gacgggcaga ccatccacaa aagcacggtg cccagccaga tcggtcccgc gctggacgcc gccaaccgcg gactggcggt gctgccgcgg gacaagatcg cctcgctgct ctacgaaacc tcgcaggcgg tcggtggcct ggggccgtcg ctgcggcgcc tggtggatgc cacccaggcg atcgcccacg atttccgggg cagcatcgac cacgtcgacg acatcgtcga gcgctcggca ccgatcatcg acagccaggc cgattccgct gacacgcttg ggcgttgggc cgccaacctg aacacgttgg ccgcccagac cgcgcggcag gacccggcgc tgcgcagcat cctggccaac gcggcgccga cggccgagca ggtgcgggcc acgttcggcg gtgtgcggga gtcgctgccg cagacgctgg ccaatctcga ggtcgtgatc gacatgctca agcgctatca caacggcgtc gagcaggcgt tggtgttcct gccgcagtcc ggcgcgatcg cccagtcggt gaccgcgcaa tcgccgggcc nggccgcact gggcgtcgnn gccatctcgc tcaaccagcc gccgccttgc ctgaccggnt ncctgncngn catcncagtg gcgcgcccng cngannncag cacggnnnnn tgccngnngg nncnnctgcn natcccgann gangcnnnnn ngtnnnnggg nncnncannn ccnnnnnnnn ngnnancngn nnnnnnncnn nnggnnnnnc nnnnnnncnn ntnnnnncnn nnnnccnnnn ngnnnncncn nncnnnannt nnnnnnnnng
Expected Protein Sequence
MAHHHHHHGW YYLRIPSLAG IGRYTLYAEL PQSGGLYRTA NVTYRGITIG KVTGVEPTER GARATMSIED GYRIPADAAA HVHSVSAVGE QYVDLVSTAG REPYLVDGQT IHKSTVPSQI GPALDAANRG LAVLPRDKIA SLLYETSQAV GGLGPSLRRL VDATQAIAHD FRGSIDHVDD IVERSAPIID SQADSADTLG RWAANLNTLA AQTARQDPAL RSILANAAPT AEQVRATFGG VRESLPQTLA NLEVVIDMLK RYHNGVEQAL VFLPQSGAIA QSVTAQSPGQ AALGVGAISL NQPPPCLTGF LPASQWRAPA DTSTAPLPAG TYCKIPMDAT NVVRGARNYP CVDVPGKRAA TPRECRSTEP YVPQGTNPWY GDPDQILTCP APGARCDQPV KPGLVIPAPS VDNGLNPLPA DRLPGTPPPI SDPLQRPGSG TVVCNGQQPN PCTYTPSALY DVRSGTVVGP DGVVYSVANS ATIGDEGWKT MLGQAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctggt actacctgcg gatcccgagc ctggccggta tcggccgcta caccctgtac gccgaattgc cgcaatccgg cggcctgtac cggaccgcca acgtcaccta ccggggaatc accatcggca aggtcaccgg cgtcgagccg accgagcgtg gcgcgcgggc gacgatgagc atcgaggacg gctaccggat cccggccgat gccgccgccc acgtgcattc ggtctcggcg gtgggcgagc agtacgtcga cctggtctcg accgccgggc gcgaaccgta tctggtcgac gggcagacca tccacaaaag cacggtgccc agccagatcg gtcccgcgct ggacgccgcc aaccgcggac tggcggtgct gccgcgggac aagatcgcct cgctgctcta cgaaacctcg caggcggtcg gtggcctggg gccgtcgctg cggcgcctgg tggatgccac ccaggcgatc gcccacgatt tccggggcag catcgaccac gtcgacgaca tcgtcgagcg ctcggcaccg atcatcgaca gccaggccga ttccgctgac acgcttgggc gttgggccgc caacctgaac acgttggccg cccagaccgc gcggcaggac ccggcgctgc gcagcatcct ggccaacgcg gcgccgacgg ccgagcaggt gcgggccacg ttcggcggtg tgcgggagtc gctgccgcag acgctggcca atctcgaggt cgtgatcgac atgctcaagc gctatcacaa cggcgtcgag caggcgttgg tgttcctgcc gcagtccggc gcgatcgccc agtcggtgac cgcgcaatcg ccgggccagg ccgcactggg cgtcggcgcc atctcgctca accagccgcc gccttgcctg accggtttcc tgccggcatc gcagtggcgc gccccggcgg acaccagcac ggcgccgctg ccggccggca cctactgcaa gatcccgatg gacgccacca atgtcgttcg gggagcccgc aattacccgt gcgtggacgt gccgggcaag cgggcggcca caccgcggga gtgccgcagc accgagccct atgtgccgca gggcaccaac ccctggtacg gcgaccccga ccagatactg acctgtccgg cgccgggcgc gcggtgcgac caaccggtga agccgggcct ggtgatcccc gcaccctcgg tcgacaacgg cctcaatccg ctgcccgccg accggctgcc gggcacccca ccgccgatca gcgacccgct gcagcggccg ggatcgggca ccgtggtgtg caacgggcag cagcccaacc cgtgcacgta cacccccagc gcgctctacg acgtgcgcag cggcacggtg gtgggccccg acggggtggt gtattcggtg gccaattcgg cgaccatcgg cgacgagggg tggaagacga tgctgggaca ggcccgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at