SSGCID: Target Page

MyavA.17391.a

RNA polymerase sigma-F factor (Rv3286c ortholog)

CENTER ID:	MyavA.17391.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.17391.a.B1.GE37413	full length		1	241

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.4181
RefSeq:	YP_883398.1
UniProt:	A0A0H2ZX82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFRELATAEP DSMEFQRQRD KIVERCLPLA DHIARRFEGR GEPRDDLVQV ARVGLVNAVV RFDVDAGSDF VSFAVPTIMG EVRRHFRDNS WSVKVPRRLK ELHLRLGTAT ADLSQRLGRA HTATELAAEL EMDREEVVEG LVAGSSYNTL SIDSGGGSEE EEVRAIADTL GDVDTGLDRI EDQESLRPLL EALPERERTV LVLRFFESMT QTQIAERVGI SQMHVSRLLA KSLTRLRDQL Q

NT Sequence

atgttccgtg agttggccac tgccgagccc gactcgatgg agtttcagcg tcaacgggac aagatcgtcg agcggtgcct gccgctggcc gaccacattg cgcgccgctt cgagggccgc ggcgagcccc gcgacgacct ggttcaggtg gcccgggtcg gcctggtcaa cgcggtggtc cggttcgacg tcgacgccgg ctcggacttc gtctcgttcg cggtgcccac catcatgggt gaggttcgcc ggcacttccg ggacaacagc tggtcggtga aggtgccgcg gcggctcaag gaactgcacc tgcggctggg caccgcgacc gccgacctgt cgcagcggct gggccgggcg cacaccgcga ccgagctcgc cgcggagctg gagatggacc gcgaagaggt ggtcgagggg ctggtcgccg gcagctccta caacaccttg tccatcgaca gcggcggcgg cagcgaagaa gaggaagtgc gcgcgatcgc cgacacgctg ggcgacgtgg acaccgggct ggaccgcatc gaggaccagg agtcgctgcg gccgttgctc gaggcgctgc ccgaacgcga acgcaccgtg ctggtgttgc ggttcttcga gtcgatgacc caaacccaga tcgccgagcg ggtgggcatc tcgcagatgc acgtgtcgcg gctgctggcc aagtcgctaa cgcggcttcg tgaccaattg cagtag

Details for MyavA.17391.a.B1.GE37413

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	28kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF RELATAEPDS MEFQRQRDKI VERCLPLADH IARRFEGRGE PRDDLVQVAR VGLVNAVVRF DVDAGSDFVS FAVPTIMGEV RRHFRDNSWS VKVPRRLKEL HLRLGTATAD LSQRLGRAPT ATELAAELEM DREEVVEGLV AGSSYNTLSI DSGGGSEEEE VRAIADTLGD VDTGLDRIED QESLRPLLEA LPERERTVLV LRFFESMTQT QIAERVGISQ MHVSRLLAKS LTRLRDQLQ

Validated NT Sequence

atggctcacc accaccacca ccatatgttc cgtgagttgg ccactgccga gcccgactcg atggagtttc agcgtcaacg ggacaagatc gtcgagcggt gcctgccgct ggccgaccac attgcgcgcc gcttcgaggg ccgcggcgag ccccgcgacg acctggttca ggtggcccgg gtcggcctgg tcaacgcggt ggtccggttc gacgtcgacg ccggctcgga cttcgtctcg ttcgcggtgc ccaccatcat gggtgaggtt cgccggcact tccgggacaa cagctggtcg gtgaaggtgc cgcggcggct caaggaactg cacctgcggc tgggcaccgc gaccgccgac ctgtcgcagc ggctgggccg ggcgcccacc gcgaccgagc tcgccgcgga gctggagatg gaccgcgaag aggtggtcga ggggctggtc gccggcagct cctacaacac cttgtccatc gacagcggcg gcggcagcga agaagaggaa gtgcgcgcga tcgccgacac gctgggcgac gtggacaccg ggctggaccg catcgaggac caggagtcgc tgcggccgtt gctcgaggcg ctgcccgaac gcgaacgcac cgtgctggtg ttgcggttct tcgagtcgat gacccaaacc cagatcgccg agcgggtggg catctcgcag atgcacgtgt cgcggctgct ggccaagtcg ctaacgcggc ttcgtgacca attgcagtaa gtgagtaaga t

Expected Protein Sequence

MAHHHHHHMF RELATAEPDS MEFQRQRDKI VERCLPLADH IARRFEGRGE PRDDLVQVAR VGLVNAVVRF DVDAGSDFVS FAVPTIMGEV RRHFRDNSWS VKVPRRLKEL HLRLGTATAD LSQRLGRAHT ATELAAELEM DREEVVEGLV AGSSYNTLSI DSGGGSEEEE VRAIADTLGD VDTGLDRIED QESLRPLLEA LPERERTVLV LRFFESMTQT QIAERVGISQ MHVSRLLAKS LTRLRDQLQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcc gtgagttggc cactgccgag cccgactcga tggagtttca gcgtcaacgg gacaagatcg tcgagcggtg cctgccgctg gccgaccaca ttgcgcgccg cttcgagggc cgcggcgagc cccgcgacga cctggttcag gtggcccggg tcggcctggt caacgcggtg gtccggttcg acgtcgacgc cggctcggac ttcgtctcgt tcgcggtgcc caccatcatg ggtgaggttc gccggcactt ccgggacaac agctggtcgg tgaaggtgcc gcggcggctc aaggaactgc acctgcggct gggcaccgcg accgccgacc tgtcgcagcg gctgggccgg gcgcacaccg cgaccgagct cgccgcggag ctggagatgg accgcgaaga ggtggtcgag gggctggtcg ccggcagctc ctacaacacc ttgtccatcg acagcggcgg cggcagcgaa gaagaggaag tgcgcgcgat cgccgacacg ctgggcgacg tggacaccgg gctggaccgc atcgaggacc aggagtcgct gcggccgttg ctcgaggcgc tgcccgaacg cgaacgcacc gtgctggtgt tgcggttctt cgagtcgatg acccaaaccc agatcgccga gcgggtgggc atctcgcaga tgcacgtgtc gcggctgctg gccaagtcgc taacgcggct tcgtgaccaa ttgcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t