MyavA.11416.c

CobQ/CobB/MinD/ParA nucleotide binding domain (Rv3918c ortholog)

CENTER ID: MyavA.11416.c
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.11416.c.B1.GE37412 full length 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.11416.c.B1.PW37407 full length 1 365
MyavA.11416.c.B1.PW37408 full length 1 365

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.5210
RefSeq: YP_884417.1
UniProt: A0A0H2ZU36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSSGDWPVA SSEAREGAAT PAPDASSATH TPAAPIGPGA PSGTPGASAV ASPAPTAATA PPSSAAAPAP PPANVSRETA DEFDTPIGAA AERAMRILHT TYPPLRRPKH RRVFTVANQK GGVGKTTTAV NLAAALALQG LKTLVIDLDP QGNASTALGI TDRQSGTPSS YEVLLGEVSV HDALRQSPHN ERLFCIPATI DLAGAEIELV SMVARENRLR TALADLDNLD FDCVFIDCPP SLGLLTINAL VAAPEVMIPI QCEYYALEGV SQLMRNIEMV KAHLNPQLEV STVVLTMYDG RTKLADQVAE EVRRYFGSKV LRTVIPRSVK VSEAPGYSMT IIDYDPGSRG AMSYLDASRE LAERD
NT Sequence
atgacttctt caggagattg gccggtcgct tcttcggagg cgcgggaggg cgcggcgacg cccgcgccgg acgcatcgag cgcgacgcac accccggcgg cacccattgg gccgggggcc ccgtcgggca cgccgggggc gtcggcggtt gcatccccgg cgccgaccgc ggccacggcg ccgccgtcat ccgccgccgc gcccgccccg ccgccggcca atgtttcacg tgaaacagcg gacgaattcg acaccccgat cggcgcggcg gccgagcgcg cgatgcggat cctccacacc acctatccgc cgctacgccg gcccaagcat cgccgcgtct tcaccgtcgc gaaccagaag ggcggcgtgg gcaagacgac gaccgccgtc aacctcgccg ccgcgctcgc cctgcaggga ctcaaaaccc tcgtcatcga cctcgacccg cagggcaacg ccagcaccgc gctcgggatc acggaccggc agtcgggtac gccgtcgtcc tacgaggtcc tgctcggcga ggtgtcggtg cacgacgcgt tgcggcagag cccgcacaac gagcgactgt tctgcatccc ggcgaccatc gacctggccg gcgccgagat cgagttggtg agcatggtgg cgcgggagaa ccggctgcgg accgcgctgg ccgatctgga caacctcgat ttcgactgcg tcttcatcga ctgcccaccg tcgctggggc tgctgaccat caacgcgctc gtcgccgccc cggaggtgat gatcccgatc cagtgcgagt actacgccct cgagggcgtg tcgcagctga tgcgcaacat cgagatggtg aaggcgcacc tcaacccgca gctcgaagtg agcaccgtgg tgctgaccat gtacgacggc cgcaccaagc tcgcggacca ggtggccgaa gaggtgcgcc gctacttcgg cagcaaggtg ctgcggacgg tgatcccgcg cagcgtcaag gtgtccgagg cgcccggcta cagcatgacc atcatcgact acgaccccgg gtcgcgcggg gcgatgagct atctcgatgc gagccgcgaa ctcgccgagc gtgattga
Details for MyavA.11416.c.B1.GE37412
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 39kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 70
Validated AA Sequence
MRILHTTYPP LRRPKHRRVF TVANQKGGVG KTTTAVNLAA ALALQGLKTL VIDLDPQGNA STALGITDRQ SGTPSSYEVL LGEVSVHDAL RQSPHNERLF CIPATIDLAG AEIELVSMVA RENRLRTALA DLDNLDFDCV FIDCPPSLGL LTINALVAAP EVMIPIQCEY YALEGVSQLM RNIEMVKAHL NPQLEVSTVV LTMYDGRTKL ADQVAEEVRR YFGSKVLRTV IPRSVKVSEA PGYSMTIIDY DPGSRGAMSY LD
Validated NT Sequence
gcatcgagat agctcatcgc cccgcgcgac ccggggtcgt agtcgatgat ggtcatgctg tagccgggcg cctcggacac cttgacgctg cgcgggatca ccgtccgcag caccttgctg ccgaagtagc ggcgcacctc ttcggccacc tggtccgcga gcttggtgcg gccgtcgtac atggtcagca ccacggtgct cacttcgagc tgcgggttga ggtgcgcctt caccatctcg atgttgcgca tcagctgcga cacgccctcg agggcgtagt actcgcactg gatcgggatc atcacctccg gggcggcgac gagcgcgttg atggtcagca gccccagcga cggtgggcag tcgatgaaga cgcagtcgaa atcgaggttg tccagatcgg ccagcgcggt ccgcagccgg ttctcccgcg ccaccatgct caccaactcg atctcggcgc cggccaggtc gatggtcgcc gggatgcaga acagtcgctc gttgtgcggg ctctgccgca acgcgtcgtg caccgacacc tcgccgagca ggacctcgta ggacgacggc gtacccgact gccggtccgt gatcccgagc gcggtgctgg cgttgccctg cgggtcgagg tcgatgacga gggttttgag tccctgcagg gcgagcgcgg cggcgaggtt gacggcggtc gtcgtcttgc ccacgccgcc cttctggttc gcgacggtga agacgcggcg atgcttgggc cggcgtagcg gcggataggt ggtgtggagg atccgcatcg cgcgctcggc cgccgcgccg atcggggtgt cgaattcgtc cgctgtttca cgtgaaacat tggccggcgg cggggcgggc gcggcggcgg atgacggcgg cgccgtggcc gcggtcggcg ccggggatgc aaccgccgac gcccccggcg tgcccgacgg ggcccccggc ccaatgggtg ccgccggggt gtgcgtcgcg ctcgatgcgt ccggcgcggg cgtcgccgcg ccctcccgcg cctccgaaga agcgaccggc caatctcctg aa
Expected Protein Sequence
MAHHHHHHMT SSGDWPVASS EAREGAATPA PDASSATHTP AAPIGPGAPS GTPGASAVAS PAPTAATAPP SSAAAPAPPP ANVSRETADE FDTPIGAAAE RAMRILHTTY PPLRRPKHRR VFTVANQKGG VGKTTTAVNL AAALALQGLK TLVIDLDPQG NASTALGITD RQSGTPSSYE VLLGEVSVHD ALRQSPHNER LFCIPATIDL AGAEIELVSM VARENRLRTA LADLDNLDFD CVFIDCPPSL GLLTINALVA APEVMIPIQC EYYALEGVSQ LMRNIEMVKA HLNPQLEVST VVLTMYDGRT KLADQVAEEV RRYFGSKVLR TVIPRSVKVS EAPGYSMTII DYDPGSRGAM SYLDASRELA ERD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactt cttcaggaga ttggccggtc gcttcttcgg aggcgcggga gggcgcggcg acgcccgcgc cggacgcatc gagcgcgacg cacaccccgg cggcacccat tgggccgggg gccccgtcgg gcacgccggg ggcgtcggcg gttgcatccc cggcgccgac cgcggccacg gcgccgccgt catccgccgc cgcgcccgcc ccgccgccgg ccaatgtttc acgtgaaaca gcggacgaat tcgacacccc gatcggcgcg gcggccgagc gcgcgatgcg gatcctccac accacctatc cgccgctacg ccggcccaag catcgccgcg tcttcaccgt cgcgaaccag aagggcggcg tgggcaagac gacgaccgcc gtcaacctcg ccgccgcgct cgccctgcag ggactcaaaa ccctcgtcat cgacctcgac ccgcagggca acgccagcac cgcgctcggg atcacggacc ggcagtcggg tacgccgtcg tcctacgagg tcctgctcgg cgaggtgtcg gtgcacgacg cgttgcggca gagcccgcac aacgagcgac tgttctgcat cccggcgacc atcgacctgg ccggcgccga gatcgagttg gtgagcatgg tggcgcggga gaaccggctg cggaccgcgc tggccgatct ggacaacctc gatttcgact gcgtcttcat cgactgccca ccgtcgctgg ggctgctgac catcaacgcg ctcgtcgccg ccccggaggt gatgatcccg atccagtgcg agtactacgc cctcgagggc gtgtcgcagc tgatgcgcaa catcgagatg gtgaaggcgc acctcaaccc gcagctcgaa gtgagcaccg tggtgctgac catgtacgac ggccgcacca agctcgcgga ccaggtggcc gaagaggtgc gccgctactt cggcagcaag gtgctgcgga cggtgatccc gcgcagcgtc aaggtgtccg aggcgcccgg ctacagcatg accatcatcg actacgaccc cgggtcgcgc ggggcgatga gctatctcga tgcgagccgc gaactcgccg agcgtgattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MyavA.11416.c.B1.PW37407
PURIFICATION DATe: 12/2/2013
CONCENTRATION: 13.2mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 70
Protocol Notes
notes unavailable
Validated AA Sequence
MRILHTTYPP LRRPKHRRVF TVANQKGGVG KTTTAVNLAA ALALQGLKTL VIDLDPQGNA STALGITDRQ SGTPSSYEVL LGEVSVHDAL RQSPHNERLF CIPATIDLAG AEIELVSMVA RENRLRTALA DLDNLDFDCV FIDCPPSLGL LTINALVAAP EVMIPIQCEY YALEGVSQLM RNIEMVKAHL NPQLEVSTVV LTMYDGRTKL ADQVAEEVRR YFGSKVLRTV IPRSVKVSEA PGYSMTIIDY DPGSRGAMSY LD
Validated NT Sequence
gcatcgagat agctcatcgc cccgcgcgac ccggggtcgt agtcgatgat ggtcatgctg tagccgggcg cctcggacac cttgacgctg cgcgggatca ccgtccgcag caccttgctg ccgaagtagc ggcgcacctc ttcggccacc tggtccgcga gcttggtgcg gccgtcgtac atggtcagca ccacggtgct cacttcgagc tgcgggttga ggtgcgcctt caccatctcg atgttgcgca tcagctgcga cacgccctcg agggcgtagt actcgcactg gatcgggatc atcacctccg gggcggcgac gagcgcgttg atggtcagca gccccagcga cggtgggcag tcgatgaaga cgcagtcgaa atcgaggttg tccagatcgg ccagcgcggt ccgcagccgg ttctcccgcg ccaccatgct caccaactcg atctcggcgc cggccaggtc gatggtcgcc gggatgcaga acagtcgctc gttgtgcggg ctctgccgca acgcgtcgtg caccgacacc tcgccgagca ggacctcgta ggacgacggc gtacccgact gccggtccgt gatcccgagc gcggtgctgg cgttgccctg cgggtcgagg tcgatgacga gggttttgag tccctgcagg gcgagcgcgg cggcgaggtt gacggcggtc gtcgtcttgc ccacgccgcc cttctggttc gcgacggtga agacgcggcg atgcttgggc cggcgtagcg gcggataggt ggtgtggagg atccgcatcg cgcgctcggc cgccgcgccg atcggggtgt cgaattcgtc cgctgtttca cgtgaaacat tggccggcgg cggggcgggc gcggcggcgg atgacggcgg cgccgtggcc gcggtcggcg ccggggatgc aaccgccgac gcccccggcg tgcccgacgg ggcccccggc ccaatgggtg ccgccggggt gtgcgtcgcg ctcgatgcgt ccggcgcggg cgtcgccgcg ccctcccgcg cctccgaaga agcgaccggc caatctcctg aa
Expressed Protein Sequence
MAHHHHHHMT SSGDWPVASS EAREGAATPA PDASSATHTP AAPIGPGAPS GTPGASAVAS PAPTAATAPP SSAAAPAPPP ANVSRETADE FDTPIGAAAE RAMRILHTTY PPLRRPKHRR VFTVANQKGG VGKTTTAVNL AAALALQGLK TLVIDLDPQG NASTALGITD RQSGTPSSYE VLLGEVSVHD ALRQSPHNER LFCIPATIDL AGAEIELVSM VARENRLRTA LADLDNLDFD CVFIDCPPSL GLLTINALVA APEVMIPIQC EYYALEGVSQ LMRNIEMVKA HLNPQLEVST VVLTMYDGRT KLADQVAEEV RRYFGSKVLR TVIPRSVKVS EAPGYSMTII DYDPGSRGAM SYLDASRELA ERD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTT CTTCAGGAGA TTGGCCGGTC GCTTCTTCGG AGGCGCGGGA GGGCGCGGCG ACGCCCGCGC CGGACGCATC GAGCGCGACG CACACCCCGG CGGCACCCAT TGGGCCGGGG GCCCCGTCGG GCACGCCGGG GGCGTCGGCG GTTGCATCCC CGGCGCCGAC CGCGGCCACG GCGCCGCCGT CATCCGCCGC CGCGCCCGCC CCGCCGCCGG CCAATGTTTC ACGTGAAACA GCGGACGAAT TCGACACCCC GATCGGCGCG GCGGCCGAGC GCGCGATGCG GATCCTCCAC ACCACCTATC CGCCGCTACG CCGGCCCAAG CATCGCCGCG TCTTCACCGT CGCGAACCAG AAGGGCGGCG TGGGCAAGAC GACGACCGCC GTCAACCTCG CCGCCGCGCT CGCCCTGCAG GGACTCAAAA CCCTCGTCAT CGACCTCGAC CCGCAGGGCA ACGCCAGCAC CGCGCTCGGG ATCACGGACC GGCAGTCGGG TACGCCGTCG TCCTACGAGG TCCTGCTCGG CGAGGTGTCG GTGCACGACG CGTTGCGGCA GAGCCCGCAC AACGAGCGAC TGTTCTGCAT CCCGGCGACC ATCGACCTGG CCGGCGCCGA GATCGAGTTG GTGAGCATGG TGGCGCGGGA GAACCGGCTG CGGACCGCGC TGGCCGATCT GGACAACCTC GATTTCGACT GCGTCTTCAT CGACTGCCCA CCGTCGCTGG GGCTGCTGAC CATCAACGCG CTCGTCGCCG CCCCGGAGGT GATGATCCCG ATCCAGTGCG AGTACTACGC CCTCGAGGGC GTGTCGCAGC TGATGCGCAA CATCGAGATG GTGAAGGCGC ACCTCAACCC GCAGCTCGAA GTGAGCACCG TGGTGCTGAC CATGTACGAC GGCCGCACCA AGCTCGCGGA CCAGGTGGCC GAAGAGGTGC GCCGCTACTT CGGCAGCAAG GTGCTGCGGA CGGTGATCCC GCGCAGCGTC AAGGTGTCCG AGGCGCCCGG CTACAGCATG ACCATCATCG ACTACGACCC CGGGTCGCGC GGGGCGATGA GCTATCTCGA TGCGAGCCGC GAACTCGCCG AGCGTGATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for MyavA.11416.c.B1.PW37408
PURIFICATION DATe: 12/2/2013
CONCENTRATION: 21.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 70
Protocol Notes
notes unavailable
Validated AA Sequence
MRILHTTYPP LRRPKHRRVF TVANQKGGVG KTTTAVNLAA ALALQGLKTL VIDLDPQGNA STALGITDRQ SGTPSSYEVL LGEVSVHDAL RQSPHNERLF CIPATIDLAG AEIELVSMVA RENRLRTALA DLDNLDFDCV FIDCPPSLGL LTINALVAAP EVMIPIQCEY YALEGVSQLM RNIEMVKAHL NPQLEVSTVV LTMYDGRTKL ADQVAEEVRR YFGSKVLRTV IPRSVKVSEA PGYSMTIIDY DPGSRGAMSY LD
Validated NT Sequence
gcatcgagat agctcatcgc cccgcgcgac ccggggtcgt agtcgatgat ggtcatgctg tagccgggcg cctcggacac cttgacgctg cgcgggatca ccgtccgcag caccttgctg ccgaagtagc ggcgcacctc ttcggccacc tggtccgcga gcttggtgcg gccgtcgtac atggtcagca ccacggtgct cacttcgagc tgcgggttga ggtgcgcctt caccatctcg atgttgcgca tcagctgcga cacgccctcg agggcgtagt actcgcactg gatcgggatc atcacctccg gggcggcgac gagcgcgttg atggtcagca gccccagcga cggtgggcag tcgatgaaga cgcagtcgaa atcgaggttg tccagatcgg ccagcgcggt ccgcagccgg ttctcccgcg ccaccatgct caccaactcg atctcggcgc cggccaggtc gatggtcgcc gggatgcaga acagtcgctc gttgtgcggg ctctgccgca acgcgtcgtg caccgacacc tcgccgagca ggacctcgta ggacgacggc gtacccgact gccggtccgt gatcccgagc gcggtgctgg cgttgccctg cgggtcgagg tcgatgacga gggttttgag tccctgcagg gcgagcgcgg cggcgaggtt gacggcggtc gtcgtcttgc ccacgccgcc cttctggttc gcgacggtga agacgcggcg atgcttgggc cggcgtagcg gcggataggt ggtgtggagg atccgcatcg cgcgctcggc cgccgcgccg atcggggtgt cgaattcgtc cgctgtttca cgtgaaacat tggccggcgg cggggcgggc gcggcggcgg atgacggcgg cgccgtggcc gcggtcggcg ccggggatgc aaccgccgac gcccccggcg tgcccgacgg ggcccccggc ccaatgggtg ccgccggggt gtgcgtcgcg ctcgatgcgt ccggcgcggg cgtcgccgcg ccctcccgcg cctccgaaga agcgaccggc caatctcctg aa
Expressed Protein Sequence
MAHHHHHHMT SSGDWPVASS EAREGAATPA PDASSATHTP AAPIGPGAPS GTPGASAVAS PAPTAATAPP SSAAAPAPPP ANVSRETADE FDTPIGAAAE RAMRILHTTY PPLRRPKHRR VFTVANQKGG VGKTTTAVNL AAALALQGLK TLVIDLDPQG NASTALGITD RQSGTPSSYE VLLGEVSVHD ALRQSPHNER LFCIPATIDL AGAEIELVSM VARENRLRTA LADLDNLDFD CVFIDCPPSL GLLTINALVA APEVMIPIQC EYYALEGVSQ LMRNIEMVKA HLNPQLEVST VVLTMYDGRT KLADQVAEEV RRYFGSKVLR TVIPRSVKVS EAPGYSMTII DYDPGSRGAM SYLDASRELA ERD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTT CTTCAGGAGA TTGGCCGGTC GCTTCTTCGG AGGCGCGGGA GGGCGCGGCG ACGCCCGCGC CGGACGCATC GAGCGCGACG CACACCCCGG CGGCACCCAT TGGGCCGGGG GCCCCGTCGG GCACGCCGGG GGCGTCGGCG GTTGCATCCC CGGCGCCGAC CGCGGCCACG GCGCCGCCGT CATCCGCCGC CGCGCCCGCC CCGCCGCCGG CCAATGTTTC ACGTGAAACA GCGGACGAAT TCGACACCCC GATCGGCGCG GCGGCCGAGC GCGCGATGCG GATCCTCCAC ACCACCTATC CGCCGCTACG CCGGCCCAAG CATCGCCGCG TCTTCACCGT CGCGAACCAG AAGGGCGGCG TGGGCAAGAC GACGACCGCC GTCAACCTCG CCGCCGCGCT CGCCCTGCAG GGACTCAAAA CCCTCGTCAT CGACCTCGAC CCGCAGGGCA ACGCCAGCAC CGCGCTCGGG ATCACGGACC GGCAGTCGGG TACGCCGTCG TCCTACGAGG TCCTGCTCGG CGAGGTGTCG GTGCACGACG CGTTGCGGCA GAGCCCGCAC AACGAGCGAC TGTTCTGCAT CCCGGCGACC ATCGACCTGG CCGGCGCCGA GATCGAGTTG GTGAGCATGG TGGCGCGGGA GAACCGGCTG CGGACCGCGC TGGCCGATCT GGACAACCTC GATTTCGACT GCGTCTTCAT CGACTGCCCA CCGTCGCTGG GGCTGCTGAC CATCAACGCG CTCGTCGCCG CCCCGGAGGT GATGATCCCG ATCCAGTGCG AGTACTACGC CCTCGAGGGC GTGTCGCAGC TGATGCGCAA CATCGAGATG GTGAAGGCGC ACCTCAACCC GCAGCTCGAA GTGAGCACCG TGGTGCTGAC CATGTACGAC GGCCGCACCA AGCTCGCGGA CCAGGTGGCC GAAGAGGTGC GCCGCTACTT CGGCAGCAAG GTGCTGCGGA CGGTGATCCC GCGCAGCGTC AAGGTGTCCG AGGCGCCCGG CTACAGCATG ACCATCATCG ACTACGACCC CGGGTCGCGC GGGGCGATGA GCTATCTCGA TGCGAGCCGC GAACTCGCCG AGCGTGATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT